Primer fabrication using polymerase mediated oligonucleotide synthesis.
Identifieur interne : 002001 ( PubMed/Curation ); précédent : 002000; suivant : 002002Primer fabrication using polymerase mediated oligonucleotide synthesis.
Auteurs : Murray J. Cairns [Australie] ; Torsten Thomas ; Carolina E. Beltran ; Daniel TillettSource :
- BMC genomics [ 1471-2164 ] ; 2009.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : DNA Primers.
- chemical , metabolism : DNA-Directed DNA Polymerase.
- methods : Polymerase Chain Reaction, Sequence Analysis, DNA.
- Base Sequence, Gene Library, Molecular Sequence Data.
Abstract
Custom solid phase oligonucleotide synthesis is an important foundation supporting nearly every aspect of current genomics. In spite of the demand for oligonucleotide primers, their synthesis remains relatively expensive, time consuming and in many circumstances a wasteful process. In this methodology, described as polymerase mediated oligonucleotide synthesis (PMOS), a DNA polymerase is used to increase the hybridization affinity of one oligonucleotide by using another as a template for DNA synthesis. This self-assembly process provides an opportunity to instantly generate a very large number of useful gene-specific primers from a small library of simple precursors. PMOS can be used to generate primers directly in the end-users laboratory within the context of any DNA polymerase chemistry such as in PCR or sequencing reactions
DOI: 10.1186/1471-2164-10-344
PubMed: 19643029
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Beltran</name></author><author><name sortKey="Tillett, Daniel" sort="Tillett, Daniel" uniqKey="Tillett D" first="Daniel" last="Tillett">Daniel Tillett</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19643029</idno><idno type="pmid">19643029</idno><idno type="doi">10.1186/1471-2164-10-344</idno><idno type="wicri:Area/PubMed/Corpus">002001</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002001</idno><idno type="wicri:Area/PubMed/Curation">002001</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002001</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Primer fabrication using polymerase mediated oligonucleotide synthesis.</title><author><name sortKey="Cairns, Murray J" sort="Cairns, Murray J" uniqKey="Cairns M" first="Murray J" last="Cairns">Murray J. Cairns</name><affiliation wicri:level="1"><nlm:affiliation>Schizophrenia Research Institute, Sydney, NSW 2006, Australia. murray.cairns@newcastle.edu.au</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Schizophrenia Research Institute, Sydney, NSW 2006</wicri:regionArea></affiliation></author><author><name sortKey="Thomas, Torsten" sort="Thomas, Torsten" uniqKey="Thomas T" first="Torsten" last="Thomas">Torsten Thomas</name></author><author><name sortKey="Beltran, Carolina E" sort="Beltran, Carolina E" uniqKey="Beltran C" first="Carolina E" last="Beltran">Carolina E. Beltran</name></author><author><name sortKey="Tillett, Daniel" sort="Tillett, Daniel" uniqKey="Tillett D" first="Daniel" last="Tillett">Daniel Tillett</name></author></analytic><series><title level="j">BMC genomics</title><idno type="eISSN">1471-2164</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>DNA Primers (chemistry)</term><term>DNA-Directed DNA Polymerase (metabolism)</term><term>Gene Library</term><term>Molecular Sequence Data</term><term>Polymerase Chain Reaction (methods)</term><term>Sequence Analysis, DNA (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN ()</term><term>Analyse de séquence d'ADN ()</term><term>Banque de gènes</term><term>DNA-directed DNA polymerase (métabolisme)</term><term>Données de séquences moléculaires</term><term>Réaction de polymérisation en chaîne ()</term><term>Séquence nucléotidique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA Primers</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA-Directed DNA Polymerase</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>DNA-directed DNA polymerase</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Gene Library</term><term>Molecular Sequence Data</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term><term>Analyse de séquence d'ADN</term><term>Banque de gènes</term><term>Données de séquences moléculaires</term><term>Réaction de polymérisation en chaîne</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Custom solid phase oligonucleotide synthesis is an important foundation supporting nearly every aspect of current genomics. In spite of the demand for oligonucleotide primers, their synthesis remains relatively expensive, time consuming and in many circumstances a wasteful process. In this methodology, described as polymerase mediated oligonucleotide synthesis (PMOS), a DNA polymerase is used to increase the hybridization affinity of one oligonucleotide by using another as a template for DNA synthesis. This self-assembly process provides an opportunity to instantly generate a very large number of useful gene-specific primers from a small library of simple precursors. PMOS can be used to generate primers directly in the end-users laboratory within the context of any DNA polymerase chemistry such as in PCR or sequencing reactions</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19643029</PMID><DateCompleted><Year>2009</Year><Month>09</Month><Day>30</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2164</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><PubDate><Year>2009</Year><Month>Jul</Month><Day>31</Day></PubDate></JournalIssue><Title>BMC genomics</Title><ISOAbbreviation>BMC Genomics</ISOAbbreviation></Journal><ArticleTitle>Primer fabrication using polymerase mediated oligonucleotide synthesis.</ArticleTitle><Pagination><MedlinePgn>344</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/1471-2164-10-344</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Custom solid phase oligonucleotide synthesis is an important foundation supporting nearly every aspect of current genomics. In spite of the demand for oligonucleotide primers, their synthesis remains relatively expensive, time consuming and in many circumstances a wasteful process. In this methodology, described as polymerase mediated oligonucleotide synthesis (PMOS), a DNA polymerase is used to increase the hybridization affinity of one oligonucleotide by using another as a template for DNA synthesis. This self-assembly process provides an opportunity to instantly generate a very large number of useful gene-specific primers from a small library of simple precursors. PMOS can be used to generate primers directly in the end-users laboratory within the context of any DNA polymerase chemistry such as in PCR or sequencing reactions</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">To demonstrate the utility of PMOS, a universal 768-member oligonucleotide library (UniSeq) was designed, fabricated and its performance optimized and evaluated in a range of PCR and DNA sequencing reactions. This methodology used to derive specific 11-mers, performed well in each of these activities and produced the desired amplification or sequencing analysis with results comparable to primers made by time consuming and expensive custom synthesis.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">On the basis of these experiments, we believe this novel system would be broadly applicable and could in many circumstances replace the need for conventional oligonucleotide synthesis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cairns</LastName><ForeName>Murray J</ForeName><Initials>MJ</Initials><AffiliationInfo><Affiliation>Schizophrenia Research Institute, Sydney, NSW 2006, Australia. murray.cairns@newcastle.edu.au</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Thomas</LastName><ForeName>Torsten</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Beltran</LastName><ForeName>Carolina E</ForeName><Initials>CE</Initials></Author><Author ValidYN="Y"><LastName>Tillett</LastName><ForeName>Daniel</ForeName><Initials>D</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>07</Month><Day>31</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>BMC Genomics</MedlineTA><NlmUniqueID>100965258</NlmUniqueID><ISSNLinking>1471-2164</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.7.7</RegistryNumber><NameOfSubstance UI="D004259">DNA-Directed DNA Polymerase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004259" MajorTopicYN="N">DNA-Directed DNA Polymerase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015723" MajorTopicYN="Y">Gene Library</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName><QualifierName UI="Q000379" 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