A truncated receptor-binding domain of MERS-CoV spike protein potently inhibits MERS-CoV infection and induces strong neutralizing antibody responses: implication for developing therapeutics and vaccines.
Identifieur interne : 001B17 ( PubMed/Curation ); précédent : 001B16; suivant : 001B18A truncated receptor-binding domain of MERS-CoV spike protein potently inhibits MERS-CoV infection and induces strong neutralizing antibody responses: implication for developing therapeutics and vaccines.
Auteurs : Lanying Du [États-Unis] ; Zhihua Kou ; Cuiqing Ma ; Xinrong Tao ; Lili Wang ; Guangyu Zhao ; Yaoqing Chen ; Fei Yu ; Chien-Te K. Tseng ; Yusen Zhou ; Shibo JiangSource :
- PloS one [ 1932-6203 ] ; 2013.
Descripteurs français
- KwdFr :
- Animaux, Anticorps neutralisants (immunologie), Coronavirus (physiologie), Cristallographie aux rayons X, Femelle, Glycoprotéine de spicule des coronavirus (métabolisme), Humains, Immunoglobuline G (métabolisme), Infections de l'appareil respiratoire (immunologie), Infections de l'appareil respiratoire (traitement médicamenteux), Infections de l'appareil respiratoire (virologie), Production d'anticorps (immunologie), Protéines recombinantes (immunologie), Récepteur Fc (métabolisme), Souris, Souris de lignée BALB C, Spécificité des anticorps (immunologie), Structure tertiaire des protéines, Sérums immuns (immunologie), Vaccination, Vaccins (immunologie), Vaccins antiviraux (immunologie), Vaccins antiviraux (usage thérapeutique).
- MESH :
- immunologie : Anticorps neutralisants, Infections de l'appareil respiratoire, Production d'anticorps, Protéines recombinantes, Spécificité des anticorps, Sérums immuns, Vaccins, Vaccins antiviraux.
- métabolisme : Glycoprotéine de spicule des coronavirus, Immunoglobuline G, Récepteur Fc.
- physiologie : Coronavirus.
- traitement médicamenteux : Infections de l'appareil respiratoire.
- usage thérapeutique : Vaccins antiviraux.
- virologie : Infections de l'appareil respiratoire.
- Animaux, Cristallographie aux rayons X, Femelle, Humains, Souris, Souris de lignée BALB C, Structure tertiaire des protéines, Vaccination.
English descriptors
- KwdEn :
- Animals, Antibodies, Neutralizing (immunology), Antibody Formation (immunology), Antibody Specificity (immunology), Coronavirus (physiology), Crystallography, X-Ray, Female, Humans, Immune Sera (immunology), Immunoglobulin G (metabolism), Mice, Mice, Inbred BALB C, Protein Structure, Tertiary, Receptors, Fc (metabolism), Recombinant Proteins (immunology), Respiratory Tract Infections (drug therapy), Respiratory Tract Infections (immunology), Respiratory Tract Infections (virology), Spike Glycoprotein, Coronavirus (metabolism), Vaccination, Vaccines (immunology), Viral Vaccines (immunology), Viral Vaccines (therapeutic use).
- MESH :
- chemical , immunology : Antibodies, Neutralizing, Immune Sera, Recombinant Proteins, Vaccines, Viral Vaccines.
- drug therapy : Respiratory Tract Infections.
- immunology : Antibody Formation, Antibody Specificity, Respiratory Tract Infections.
- chemical , metabolism : Immunoglobulin G, Receptors, Fc, Spike Glycoprotein, Coronavirus.
- physiology : Coronavirus.
- chemical , therapeutic use : Viral Vaccines.
- virology : Respiratory Tract Infections.
- Animals, Crystallography, X-Ray, Female, Humans, Mice, Mice, Inbred BALB C, Protein Structure, Tertiary, Vaccination.
Abstract
An emerging respiratory infectious disease with high mortality, Middle East respiratory syndrome (MERS), is caused by a novel coronavirus (MERS-CoV). It was first reported in 2012 in Saudi Arabia and has now spread to eight countries. Development of effective therapeutics and vaccines is crucial to save lives and halt the spread of MERS-CoV. Here, we show that a recombinant protein containing a 212-amino acid fragment (residues 377-588) in the truncated receptor-binding domain (RBD: residues 367-606) of MERS-CoV spike (S) protein fused with human IgG Fc fragment (S377-588-Fc) is highly expressed in the culture supernatant of transfected 293T cells. The purified S377-588-Fc protein efficiently binds to dipeptidyl peptidase 4 (DPP4), the receptor of MERS-CoV, and potently inhibited MERS-CoV infection, suggesting its potential to be further developed as a therapeutic modality for treating MERS-CoV infection and saving the patients' lives. The recombinant S377-588-Fc is able to induce in the vaccinated mice strong MERS-CoV S-specific antibodies, which blocks the binding of RBD to DPP4 receptor and effectively neutralizes MERS-CoV infection. These findings indicate that this truncated RBD protein shows promise for further development as an effective and safe vaccine for the prevention of MERS-CoV infection.
DOI: 10.1371/journal.pone.0081587
PubMed: 24324708
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001B17
Links to Exploration step
pubmed:24324708Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A truncated receptor-binding domain of MERS-CoV spike protein potently inhibits MERS-CoV infection and induces strong neutralizing antibody responses: implication for developing therapeutics and vaccines.</title>
<author><name sortKey="Du, Lanying" sort="Du, Lanying" uniqKey="Du L" first="Lanying" last="Du">Lanying Du</name>
<affiliation wicri:level="1"><nlm:affiliation>Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kou, Zhihua" sort="Kou, Zhihua" uniqKey="Kou Z" first="Zhihua" last="Kou">Zhihua Kou</name>
</author>
<author><name sortKey="Ma, Cuiqing" sort="Ma, Cuiqing" uniqKey="Ma C" first="Cuiqing" last="Ma">Cuiqing Ma</name>
</author>
<author><name sortKey="Tao, Xinrong" sort="Tao, Xinrong" uniqKey="Tao X" first="Xinrong" last="Tao">Xinrong Tao</name>
</author>
<author><name sortKey="Wang, Lili" sort="Wang, Lili" uniqKey="Wang L" first="Lili" last="Wang">Lili Wang</name>
</author>
<author><name sortKey="Zhao, Guangyu" sort="Zhao, Guangyu" uniqKey="Zhao G" first="Guangyu" last="Zhao">Guangyu Zhao</name>
</author>
<author><name sortKey="Chen, Yaoqing" sort="Chen, Yaoqing" uniqKey="Chen Y" first="Yaoqing" last="Chen">Yaoqing Chen</name>
</author>
<author><name sortKey="Yu, Fei" sort="Yu, Fei" uniqKey="Yu F" first="Fei" last="Yu">Fei Yu</name>
</author>
<author><name sortKey="Tseng, Chien Te K" sort="Tseng, Chien Te K" uniqKey="Tseng C" first="Chien-Te K" last="Tseng">Chien-Te K. Tseng</name>
</author>
<author><name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name>
</author>
<author><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2013">2013</date>
<idno type="RBID">pubmed:24324708</idno>
<idno type="pmid">24324708</idno>
<idno type="doi">10.1371/journal.pone.0081587</idno>
<idno type="wicri:Area/PubMed/Corpus">001B17</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001B17</idno>
<idno type="wicri:Area/PubMed/Curation">001B17</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001B17</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">A truncated receptor-binding domain of MERS-CoV spike protein potently inhibits MERS-CoV infection and induces strong neutralizing antibody responses: implication for developing therapeutics and vaccines.</title>
<author><name sortKey="Du, Lanying" sort="Du, Lanying" uniqKey="Du L" first="Lanying" last="Du">Lanying Du</name>
<affiliation wicri:level="1"><nlm:affiliation>Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kou, Zhihua" sort="Kou, Zhihua" uniqKey="Kou Z" first="Zhihua" last="Kou">Zhihua Kou</name>
</author>
<author><name sortKey="Ma, Cuiqing" sort="Ma, Cuiqing" uniqKey="Ma C" first="Cuiqing" last="Ma">Cuiqing Ma</name>
</author>
<author><name sortKey="Tao, Xinrong" sort="Tao, Xinrong" uniqKey="Tao X" first="Xinrong" last="Tao">Xinrong Tao</name>
</author>
<author><name sortKey="Wang, Lili" sort="Wang, Lili" uniqKey="Wang L" first="Lili" last="Wang">Lili Wang</name>
</author>
<author><name sortKey="Zhao, Guangyu" sort="Zhao, Guangyu" uniqKey="Zhao G" first="Guangyu" last="Zhao">Guangyu Zhao</name>
</author>
<author><name sortKey="Chen, Yaoqing" sort="Chen, Yaoqing" uniqKey="Chen Y" first="Yaoqing" last="Chen">Yaoqing Chen</name>
</author>
<author><name sortKey="Yu, Fei" sort="Yu, Fei" uniqKey="Yu F" first="Fei" last="Yu">Fei Yu</name>
</author>
<author><name sortKey="Tseng, Chien Te K" sort="Tseng, Chien Te K" uniqKey="Tseng C" first="Chien-Te K" last="Tseng">Chien-Te K. Tseng</name>
</author>
<author><name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name>
</author>
<author><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name>
</author>
</analytic>
<series><title level="j">PloS one</title>
<idno type="eISSN">1932-6203</idno>
<imprint><date when="2013" type="published">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antibodies, Neutralizing (immunology)</term>
<term>Antibody Formation (immunology)</term>
<term>Antibody Specificity (immunology)</term>
<term>Coronavirus (physiology)</term>
<term>Crystallography, X-Ray</term>
<term>Female</term>
<term>Humans</term>
<term>Immune Sera (immunology)</term>
<term>Immunoglobulin G (metabolism)</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Protein Structure, Tertiary</term>
<term>Receptors, Fc (metabolism)</term>
<term>Recombinant Proteins (immunology)</term>
<term>Respiratory Tract Infections (drug therapy)</term>
<term>Respiratory Tract Infections (immunology)</term>
<term>Respiratory Tract Infections (virology)</term>
<term>Spike Glycoprotein, Coronavirus (metabolism)</term>
<term>Vaccination</term>
<term>Vaccines (immunology)</term>
<term>Viral Vaccines (immunology)</term>
<term>Viral Vaccines (therapeutic use)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Anticorps neutralisants (immunologie)</term>
<term>Coronavirus (physiologie)</term>
<term>Cristallographie aux rayons X</term>
<term>Femelle</term>
<term>Glycoprotéine de spicule des coronavirus (métabolisme)</term>
<term>Humains</term>
<term>Immunoglobuline G (métabolisme)</term>
<term>Infections de l'appareil respiratoire (immunologie)</term>
<term>Infections de l'appareil respiratoire (traitement médicamenteux)</term>
<term>Infections de l'appareil respiratoire (virologie)</term>
<term>Production d'anticorps (immunologie)</term>
<term>Protéines recombinantes (immunologie)</term>
<term>Récepteur Fc (métabolisme)</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Spécificité des anticorps (immunologie)</term>
<term>Structure tertiaire des protéines</term>
<term>Sérums immuns (immunologie)</term>
<term>Vaccination</term>
<term>Vaccins (immunologie)</term>
<term>Vaccins antiviraux (immunologie)</term>
<term>Vaccins antiviraux (usage thérapeutique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Neutralizing</term>
<term>Immune Sera</term>
<term>Recombinant Proteins</term>
<term>Vaccines</term>
<term>Viral Vaccines</term>
</keywords>
<keywords scheme="MESH" qualifier="drug therapy" xml:lang="en"><term>Respiratory Tract Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps neutralisants</term>
<term>Infections de l'appareil respiratoire</term>
<term>Production d'anticorps</term>
<term>Protéines recombinantes</term>
<term>Spécificité des anticorps</term>
<term>Sérums immuns</term>
<term>Vaccins</term>
<term>Vaccins antiviraux</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Antibody Formation</term>
<term>Antibody Specificity</term>
<term>Respiratory Tract Infections</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Immunoglobulin G</term>
<term>Receptors, Fc</term>
<term>Spike Glycoprotein, Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéine de spicule des coronavirus</term>
<term>Immunoglobuline G</term>
<term>Récepteur Fc</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Coronavirus</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="therapeutic use" xml:lang="en"><term>Viral Vaccines</term>
</keywords>
<keywords scheme="MESH" qualifier="traitement médicamenteux" xml:lang="fr"><term>Infections de l'appareil respiratoire</term>
</keywords>
<keywords scheme="MESH" qualifier="usage thérapeutique" xml:lang="fr"><term>Vaccins antiviraux</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections de l'appareil respiratoire</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Respiratory Tract Infections</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Crystallography, X-Ray</term>
<term>Female</term>
<term>Humans</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Protein Structure, Tertiary</term>
<term>Vaccination</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cristallographie aux rayons X</term>
<term>Femelle</term>
<term>Humains</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Structure tertiaire des protéines</term>
<term>Vaccination</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">An emerging respiratory infectious disease with high mortality, Middle East respiratory syndrome (MERS), is caused by a novel coronavirus (MERS-CoV). It was first reported in 2012 in Saudi Arabia and has now spread to eight countries. Development of effective therapeutics and vaccines is crucial to save lives and halt the spread of MERS-CoV. Here, we show that a recombinant protein containing a 212-amino acid fragment (residues 377-588) in the truncated receptor-binding domain (RBD: residues 367-606) of MERS-CoV spike (S) protein fused with human IgG Fc fragment (S377-588-Fc) is highly expressed in the culture supernatant of transfected 293T cells. The purified S377-588-Fc protein efficiently binds to dipeptidyl peptidase 4 (DPP4), the receptor of MERS-CoV, and potently inhibited MERS-CoV infection, suggesting its potential to be further developed as a therapeutic modality for treating MERS-CoV infection and saving the patients' lives. The recombinant S377-588-Fc is able to induce in the vaccinated mice strong MERS-CoV S-specific antibodies, which blocks the binding of RBD to DPP4 receptor and effectively neutralizes MERS-CoV infection. These findings indicate that this truncated RBD protein shows promise for further development as an effective and safe vaccine for the prevention of MERS-CoV infection. </div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24324708</PMID>
<DateCompleted><Year>2014</Year>
<Month>09</Month>
<Day>11</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>8</Volume>
<Issue>12</Issue>
<PubDate><Year>2013</Year>
</PubDate>
</JournalIssue>
<Title>PloS one</Title>
<ISOAbbreviation>PLoS ONE</ISOAbbreviation>
</Journal>
<ArticleTitle>A truncated receptor-binding domain of MERS-CoV spike protein potently inhibits MERS-CoV infection and induces strong neutralizing antibody responses: implication for developing therapeutics and vaccines.</ArticleTitle>
<Pagination><MedlinePgn>e81587</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0081587</ELocationID>
<Abstract><AbstractText>An emerging respiratory infectious disease with high mortality, Middle East respiratory syndrome (MERS), is caused by a novel coronavirus (MERS-CoV). It was first reported in 2012 in Saudi Arabia and has now spread to eight countries. Development of effective therapeutics and vaccines is crucial to save lives and halt the spread of MERS-CoV. Here, we show that a recombinant protein containing a 212-amino acid fragment (residues 377-588) in the truncated receptor-binding domain (RBD: residues 367-606) of MERS-CoV spike (S) protein fused with human IgG Fc fragment (S377-588-Fc) is highly expressed in the culture supernatant of transfected 293T cells. The purified S377-588-Fc protein efficiently binds to dipeptidyl peptidase 4 (DPP4), the receptor of MERS-CoV, and potently inhibited MERS-CoV infection, suggesting its potential to be further developed as a therapeutic modality for treating MERS-CoV infection and saving the patients' lives. The recombinant S377-588-Fc is able to induce in the vaccinated mice strong MERS-CoV S-specific antibodies, which blocks the binding of RBD to DPP4 receptor and effectively neutralizes MERS-CoV infection. These findings indicate that this truncated RBD protein shows promise for further development as an effective and safe vaccine for the prevention of MERS-CoV infection. </AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Du</LastName>
<ForeName>Lanying</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York, United States of America.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kou</LastName>
<ForeName>Zhihua</ForeName>
<Initials>Z</Initials>
</Author>
<Author ValidYN="Y"><LastName>Ma</LastName>
<ForeName>Cuiqing</ForeName>
<Initials>C</Initials>
</Author>
<Author ValidYN="Y"><LastName>Tao</LastName>
<ForeName>Xinrong</ForeName>
<Initials>X</Initials>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Lili</ForeName>
<Initials>L</Initials>
</Author>
<Author ValidYN="Y"><LastName>Zhao</LastName>
<ForeName>Guangyu</ForeName>
<Initials>G</Initials>
</Author>
<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Yaoqing</ForeName>
<Initials>Y</Initials>
</Author>
<Author ValidYN="Y"><LastName>Yu</LastName>
<ForeName>Fei</ForeName>
<Initials>F</Initials>
</Author>
<Author ValidYN="Y"><LastName>Tseng</LastName>
<ForeName>Chien-Te K</ForeName>
<Initials>CT</Initials>
</Author>
<Author ValidYN="Y"><LastName>Zhou</LastName>
<ForeName>Yusen</ForeName>
<Initials>Y</Initials>
</Author>
<Author ValidYN="Y"><LastName>Jiang</LastName>
<ForeName>Shibo</ForeName>
<Initials>S</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2013</Year>
<Month>12</Month>
<Day>04</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>PLoS One</MedlineTA>
<NlmUniqueID>101285081</NlmUniqueID>
<ISSNLinking>1932-6203</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D057134">Antibodies, Neutralizing</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D007106">Immune Sera</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D007074">Immunoglobulin G</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011961">Receptors, Fc</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014612">Vaccines</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014765">Viral Vaccines</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D057134" MajorTopicYN="N">Antibodies, Neutralizing</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000917" MajorTopicYN="N">Antibody Formation</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000918" MajorTopicYN="N">Antibody Specificity</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017934" MajorTopicYN="N">Coronavirus</DescriptorName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018360" MajorTopicYN="N">Crystallography, X-Ray</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007106" MajorTopicYN="N">Immune Sera</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007074" MajorTopicYN="N">Immunoglobulin G</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017434" MajorTopicYN="N">Protein Structure, Tertiary</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011961" MajorTopicYN="N">Receptors, Fc</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012141" MajorTopicYN="N">Respiratory Tract Infections</DescriptorName>
<QualifierName UI="Q000188" MajorTopicYN="N">drug therapy</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D064370" MajorTopicYN="N">Spike Glycoprotein, Coronavirus</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014611" MajorTopicYN="N">Vaccination</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014612" MajorTopicYN="N">Vaccines</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014765" MajorTopicYN="N">Viral Vaccines</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
<QualifierName UI="Q000627" MajorTopicYN="N">therapeutic use</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year>
<Month>09</Month>
<Day>28</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2013</Year>
<Month>10</Month>
<Day>22</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2013</Year>
<Month>12</Month>
<Day>11</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2013</Year>
<Month>12</Month>
<Day>11</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2014</Year>
<Month>9</Month>
<Day>12</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">24324708</ArticleId>
<ArticleId IdType="doi">10.1371/journal.pone.0081587</ArticleId>
<ArticleId IdType="pii">PONE-D-13-39799</ArticleId>
<ArticleId IdType="pmc">PMC3852489</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1977-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12671062</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 Aug;87(15):8638-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23720729</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2003 Jun 20;300(5627):1961-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12766206</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2003 Nov 27;426(6965):450-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14647384</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2004 Jan 30;279(5):3197-201</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14670965</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2004 Nov 12;324(2):773-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15474494</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 1993 Sep 9;365(6442):113</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8371754</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1994 Oct 11;91(21):9770-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7937889</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2005 Sep 16;309(5742):1864-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16166518</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Immunol. 2006 May 15;176(10):6085-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16670317</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 2009 Feb 5;27(6):857-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19084043</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Microbiol. 2009 Mar;7(3):226-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19198616</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2009 Oct 10;393(1):144-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19683779</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Microbiol Biotechnol. 2010 Jun;87(2):401-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20422181</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2011 Mar 15;108(11):4388-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21368166</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2012 Nov 8;367(19):1814-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23075143</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Med. 2013 Aug;19(8):952</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23921729</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2013 Aug 8;500(7461):227-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23831647</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 Sep;87(17):9939-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23824801</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 Sep;87(17):9953-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23824802</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 2013 Aug 24;382(9893):694-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23831141</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 Oct;87(19):10777-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23903833</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioengineered. 2013 Sep-Oct;4(5):305-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23644447</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Infect. 2012 Dec;65(6):477-89</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23072791</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cancer Control. 2013 Jan;20(1):22-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23302904</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2013;8(1):e53568</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23320093</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2013 Mar 14;495(7440):251-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23486063</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Euro Surveill. 2013;18(11):20427</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23517868</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Infect. 2013 May;66(5):464-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23266463</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2013 Apr 18;368(16):1560-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23550601</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 May;87(10):5502-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23468491</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Infect Dis. 2013 Jun 1;207(11):1743-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23532101</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2013;8(7):e69127</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23844250</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microbes Infect. 2013 Jul-Aug;15(8-9):625-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23791956</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 Aug;87(16):9379-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23785207</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Res. 2013 Aug;23(8):986-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23835475</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2013 Aug 1;369(5):407-16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23782161</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Euro Surveill. 2013;18(24). pii: 20502</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23787161</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Euro Surveill. 2013;18(24). pii: 20503</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23787162</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2013 Jun 27;368(26):2487-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23718156</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2003 May 29;348(22):2186-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12773645</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001B17 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd -nk 001B17 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= PubMed |étape= Curation |type= RBID |clé= pubmed:24324708 |texte= A truncated receptor-binding domain of MERS-CoV spike protein potently inhibits MERS-CoV infection and induces strong neutralizing antibody responses: implication for developing therapeutics and vaccines. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Curation/RBID.i -Sk "pubmed:24324708" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |