Isolation and quantitation of adiponectin higher order complexes.
Identifieur interne : 001A67 ( PubMed/Curation ); précédent : 001A66; suivant : 001A68Isolation and quantitation of adiponectin higher order complexes.
Auteurs : Joseph M. Rutkowski [États-Unis] ; Philipp E. Scherer [États-Unis]Source :
- Methods in enzymology [ 1557-7988 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
- anatomopathologie : Adipocytes, Tissu adipeux.
- isolement et purification : Adiponectine.
- métabolisme : Adipocytes, Glucose, Tissu adipeux.
- sang : Adipokines, Adiponectine.
- Humains, Masse moléculaire.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Adipokines, Adiponectin.
- chemical , isolation & purification : Adiponectin.
- metabolism : Adipocytes, Adipose Tissue, Glucose.
- pathology : Adipocytes, Adipose Tissue.
- Humans, Molecular Weight.
Abstract
Adiponectin is a circulating bioactive hormone secreted by adipocytes as oligomers ranging in size from 90 kDa trimers and 180 kDa hexamers to larger high molecular weight oligomers that may reach 18- or 36-mers in size. While total circulating adiponectin levels correlate well with metabolic health, it is the relative distribution of adiponectin complexes that is most clinically relevant to glucose sensitivity and inflammation. High molecular weight adiponectin best mirrors insulin sensitivity, while trimeric adiponectin dominates with insulin resistance and adipose tissue inflammation. Experimental animal and in vitro models have also linked the relative fraction of high molecular weight adiponectin to its positive effects. Quantitating adiponectin size distribution thus provides a window into metabolic health and can serve as a surrogate marker for adipose tissue fitness. Here, we present a detailed protocol for isolating and quantitating adiponectin complexes in serum or plasma that has been extensively utilized for both human clinical samples and numerous animal models under various experimental conditions. Examples are presented of different adiponectin distributions and tips are provided for optimization using available equipment. Comparison of this rigorous approach to other available methods is also discussed. In total, this summary is a blueprint for the expanded quantitation and study of adiponectin complexes.
DOI: 10.1016/B978-0-12-411619-1.00013-6
PubMed: 24480350
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<term>Adiponectin (blood)</term>
<term>Adiponectin (isolation & purification)</term>
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<term>Adipocytes (métabolisme)</term>
<term>Adipokines (sang)</term>
<term>Adiponectine (isolement et purification)</term>
<term>Adiponectine (sang)</term>
<term>Glucose (métabolisme)</term>
<term>Humains</term>
<term>Masse moléculaire</term>
<term>Tissu adipeux (anatomopathologie)</term>
<term>Tissu adipeux (métabolisme)</term>
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<front><div type="abstract" xml:lang="en">Adiponectin is a circulating bioactive hormone secreted by adipocytes as oligomers ranging in size from 90 kDa trimers and 180 kDa hexamers to larger high molecular weight oligomers that may reach 18- or 36-mers in size. While total circulating adiponectin levels correlate well with metabolic health, it is the relative distribution of adiponectin complexes that is most clinically relevant to glucose sensitivity and inflammation. High molecular weight adiponectin best mirrors insulin sensitivity, while trimeric adiponectin dominates with insulin resistance and adipose tissue inflammation. Experimental animal and in vitro models have also linked the relative fraction of high molecular weight adiponectin to its positive effects. Quantitating adiponectin size distribution thus provides a window into metabolic health and can serve as a surrogate marker for adipose tissue fitness. Here, we present a detailed protocol for isolating and quantitating adiponectin complexes in serum or plasma that has been extensively utilized for both human clinical samples and numerous animal models under various experimental conditions. Examples are presented of different adiponectin distributions and tips are provided for optimization using available equipment. Comparison of this rigorous approach to other available methods is also discussed. In total, this summary is a blueprint for the expanded quantitation and study of adiponectin complexes.</div>
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<ReferenceList><Reference><Citation>J Biol Chem. 2003 Mar 14;278(11):9073-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12496257</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Endocr J. 2013;60(1):119-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23006813</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2004 Mar 26;279(13):12152-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14699128</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 1995 Nov 10;270(45):26746-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7592907</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Diabetologia. 2005 Jun;48(6):1084-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15902402</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Diabetes. 2005 Sep;54(9):2712-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16123361</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Diabetes. 2006 Jan;55(1):249-59</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16380500</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Chim Acta. 2006 Oct;372(1-2):47-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16697359</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Circ J. 2008 Jan;72(1):23-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18159094</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem J. 2008 Feb 1;409(3):623-33</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18177270</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Chem. 2007 Dec;53(12):2144-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17951289</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Endocrinology. 2008 May;149(5):2270-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18202126</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Perinat Med. 2009;37(4):349-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19348608</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Pharmacol Ther. 2009 Dec;86(6):592-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19915603</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Atheroscler Thromb. 2011;18(7):616-27</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21512277</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Diab Vasc Dis Res. 2011 Jul;8(3):190-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21555334</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Pediatr Nephrol. 2011 Nov;26(11):2009-17</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21553321</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Carcinogenesis. 2012 Jun;33(6):1211-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22431719</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Diabetologia. 2012 Sep;55(9):2319-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22688349</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Circ Res. 2004 Mar 5;94(4):e27-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14752031</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>
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