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Performance and clinical validation of the RealStar MERS-CoV Kit for detection of Middle East respiratory syndrome coronavirus RNA.

Identifieur interne : 001A09 ( PubMed/Curation ); précédent : 001A08; suivant : 001A10

Performance and clinical validation of the RealStar MERS-CoV Kit for detection of Middle East respiratory syndrome coronavirus RNA.

Auteurs : Victor Max Corman [Allemagne] ; Stephan Ölschl Ger [Allemagne] ; Clemens-Martin Wendtner [Allemagne] ; Jan Felix Drexler [Allemagne] ; Markus Hess [Allemagne] ; Christian Drosten [Allemagne]

Source :

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology [ 1873-5967 ] ; 2014.

RBID : pubmed:24726679

Descripteurs français

English descriptors

KwdEn :
- Coronavirus Infections (diagnosis), Cross Reactions, Humans, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Molecular Diagnostic Techniques (methods), Molecular Diagnostic Techniques (standards), RNA, Viral (genetics), RNA, Viral (isolation & purification), Real-Time Polymerase Chain Reaction (methods), Real-Time Polymerase Chain Reaction (standards), Reference Standards, Sensitivity and Specificity.
MESH :
- chemical , genetics : RNA, Viral.
- diagnosis : Coronavirus Infections.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus, RNA, Viral.
- methods : Molecular Diagnostic Techniques, Real-Time Polymerase Chain Reaction.
- standards : Molecular Diagnostic Techniques, Real-Time Polymerase Chain Reaction.
- Cross Reactions, Humans, Reference Standards, Sensitivity and Specificity.

Abstract

A highly pathogenic human coronavirus causing respiratory disease emerged in the Middle East region in 2012. In-house molecular diagnostic methods for this virus termed Middle East respiratory syndrome coronavirus (MERS-CoV) allowed sensitive MERS-CoV RNA detection in patient samples. Fast diagnosis is important to manage human cases and trace possible contacts.

DOI: 10.1016/j.jcv.2014.03.012
PubMed: 24726679

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<front><div type="abstract" xml:lang="en">A highly pathogenic human coronavirus causing respiratory disease emerged in the Middle East region in 2012. In-house molecular diagnostic methods for this virus termed Middle East respiratory syndrome coronavirus (MERS-CoV) allowed sensitive MERS-CoV RNA detection in patient samples. Fast diagnosis is important to manage human cases and trace possible contacts.</div>
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<Title>Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology</Title>
<ISOAbbreviation>J. Clin. Virol.</ISOAbbreviation>
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<ArticleTitle>Performance and clinical validation of the RealStar MERS-CoV Kit for detection of Middle East respiratory syndrome coronavirus RNA.</ArticleTitle>
<Pagination><MedlinePgn>168-71</MedlinePgn>
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<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">A highly pathogenic human coronavirus causing respiratory disease emerged in the Middle East region in 2012. In-house molecular diagnostic methods for this virus termed Middle East respiratory syndrome coronavirus (MERS-CoV) allowed sensitive MERS-CoV RNA detection in patient samples. Fast diagnosis is important to manage human cases and trace possible contacts.</AbstractText>
<AbstractText Label="OBJECTIVES" NlmCategory="OBJECTIVE">The aim of this study was to improve the availability of existing nucleic acid amplification-based diagnostic methods for MERS-CoV infections by providing a real-time RT-PCR kit, including an internal control and two target regions recommended by the World Health Organization (WHO). And to validate this kit (RealStar MERS-CoV RT-PCR kit 1.0, Altona Diagnostics GmbH, Hamburg, Germany) using clinical samples of one MERS-CoV case from Munich and respiratory samples of patients with other respiratory diseases.</AbstractText>
<AbstractText Label="STUDY DESIGN" NlmCategory="METHODS">An internal amplification control was included into the RT-PCR assays targeting the genomic region upstream of the Envelope gene (upE) and within open reading frame (ORF) 1A. Based on these assays, a ready-to-use real-time RT-PCR kit featuring both the upE and ORF1A assays was developed, validated and compared to the established in-house versions.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">The performance of both RT-PCR assays included in the kit is comparable to the in-house assays. They show high analytical sensitivity (upE: 5.3 copies/reaction; ORF1A: 9.3 copies/reaction), no cross-reactivity with other respiratory pathogens and detected MERS-CoV RNA in patient samples in almost the same manner as the in-house versions.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The kit is a valuable tool for assisting in the rapid diagnosis, patient management and epidemiology of suspected MERS-CoV cases.</AbstractText>
<CopyrightInformation>Copyright © 2014 Elsevier B.V. All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Corman</LastName>
<ForeName>Victor Max</ForeName>
<Initials>VM</Initials>
<AffiliationInfo><Affiliation>Institute of Virology, University of Bonn Medical Centre, 53127 Bonn, Germany. Electronic address: corman@virology-bonn.de.</Affiliation>
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<Author ValidYN="Y"><LastName>Ölschläger</LastName>
<ForeName>Stephan</ForeName>
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<AffiliationInfo><Affiliation>Altona Diagnostics GmbH, Mörkenstrasse 12, 22767 Hamburg, Germany.</Affiliation>
</AffiliationInfo>
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<AffiliationInfo><Affiliation>Klinikum Schwabing, Munich, Germany.</Affiliation>
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<Author ValidYN="Y"><LastName>Drosten</LastName>
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<AffiliationInfo><Affiliation>Institute of Virology, University of Bonn Medical Centre, 53127 Bonn, Germany.</Affiliation>
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EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Curation

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001A09 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd -nk 001A09 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    PubMed
   |étape=   Curation
   |type=    RBID
   |clé=     pubmed:24726679
   |texte=   Performance and clinical validation of the RealStar MERS-CoV Kit for detection of Middle East respiratory syndrome coronavirus RNA.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Curation/RBID.i   -Sk "pubmed:24726679" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd   \
       | NlmPubMed2Wicri -a MersV1

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration MERS

Performance and clinical validation of the RealStar MERS-CoV Kit for detection of Middle East respiratory syndrome coronavirus RNA.

Performance and clinical validation of the RealStar MERS-CoV Kit for detection of Middle East respiratory syndrome coronavirus RNA.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

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Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki