Infectious Middle East Respiratory Syndrome Coronavirus Excretion and Serotype Variability Based on Live Virus Isolates from Patients in Saudi Arabia.
Identifieur interne : 001557 ( PubMed/Curation ); précédent : 001556; suivant : 001558Infectious Middle East Respiratory Syndrome Coronavirus Excretion and Serotype Variability Based on Live Virus Isolates from Patients in Saudi Arabia.
Auteurs : Doreen Muth [Allemagne] ; Victor M. Corman [Allemagne] ; Benjamin Meyer [Allemagne] ; Abdullah Assiri ; Malak Al-Masri ; Mohamed Farah ; Katja Steinhagen [Allemagne] ; Erik Lattwein [Allemagne] ; Jaffar A. Al-Tawfiq [États-Unis] ; Ali Albarrak ; Marcel A. Müller [Allemagne] ; Christian Drosten [Allemagne] ; Ziad A. Memish [Allemagne]Source :
- Journal of clinical microbiology [ 1098-660X ] ; 2015.
Descripteurs français
- KwdFr :
- Adulte, Adulte d'âge moyen, Animaux, Arabie saoudite (épidémiologie), Cellules Caco-2, Cellules Vero, Coronavirus du syndrome respiratoire du Moyen-Orient (), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Culture virale, Femelle, Humains, Infections à coronavirus (virologie), Infections à coronavirus (épidémiologie), Jeune adulte, Mâle, Sujet âgé, Sujet âgé de 80 ans ou plus, Sérogroupe.
- MESH :
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- virologie : Infections à coronavirus.
- épidémiologie : Arabie saoudite, Infections à coronavirus.
- Adulte, Adulte d'âge moyen, Animaux, Cellules Caco-2, Cellules Vero, Coronavirus du syndrome respiratoire du Moyen-Orient, Culture virale, Femelle, Humains, Jeune adulte, Mâle, Sujet âgé, Sujet âgé de 80 ans ou plus, Sérogroupe.
- Wicri :
- geographic : Arabie saoudite.
English descriptors
- KwdEn :
- Adult, Aged, Aged, 80 and over, Animals, Caco-2 Cells, Chlorocebus aethiops, Coronavirus Infections (epidemiology), Coronavirus Infections (virology), Female, Humans, Male, Middle Aged, Middle East Respiratory Syndrome Coronavirus (classification), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Saudi Arabia (epidemiology), Serogroup, Vero Cells, Virus Cultivation, Young Adult.
- MESH :
- geographic , epidemiology : Saudi Arabia.
- classification : Middle East Respiratory Syndrome Coronavirus.
- epidemiology : Coronavirus Infections.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- virology : Coronavirus Infections.
- Adult, Aged, Aged, 80 and over, Animals, Caco-2 Cells, Chlorocebus aethiops, Female, Humans, Male, Middle Aged, Serogroup, Vero Cells, Virus Cultivation, Young Adult.
Abstract
The newly emerged Middle East respiratory syndrome coronavirus (MERS-CoV) has infected at least 1,082 people, including 439 fatalities. So far, no empirical virus isolation study has been done to elucidate infectious virus secretion or serotype variability. Here, we used 51 respiratory samples from 32 patients with confirmed MERS-CoV infection for virus isolation in Vero B4 and Caco-2 cells. We found Caco-2 cells to significantly enhance isolation success over routinely used Vero cells. Isolation success correlated with viral RNA concentration and time after diagnosis as well as with the amount of IgA antibodies secreted in respiratory samples used for isolation. Results from plaque reduction neutralization assays using a representative range of serum samples and virus isolates suggested that all circulating human MERS-CoV strains represent one single serotype. The choice of prototype strain is not likely to influence the success of candidate MERS-CoV vaccines. However, vaccine formulations should be evaluated for their potential to induce IgA.
DOI: 10.1128/JCM.01368-15
PubMed: 26157150
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Le document en format XML
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<front><div type="abstract" xml:lang="en">The newly emerged Middle East respiratory syndrome coronavirus (MERS-CoV) has infected at least 1,082 people, including 439 fatalities. So far, no empirical virus isolation study has been done to elucidate infectious virus secretion or serotype variability. Here, we used 51 respiratory samples from 32 patients with confirmed MERS-CoV infection for virus isolation in Vero B4 and Caco-2 cells. We found Caco-2 cells to significantly enhance isolation success over routinely used Vero cells. Isolation success correlated with viral RNA concentration and time after diagnosis as well as with the amount of IgA antibodies secreted in respiratory samples used for isolation. Results from plaque reduction neutralization assays using a representative range of serum samples and virus isolates suggested that all circulating human MERS-CoV strains represent one single serotype. The choice of prototype strain is not likely to influence the success of candidate MERS-CoV vaccines. However, vaccine formulations should be evaluated for their potential to induce IgA. </div>
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<Abstract><AbstractText>The newly emerged Middle East respiratory syndrome coronavirus (MERS-CoV) has infected at least 1,082 people, including 439 fatalities. So far, no empirical virus isolation study has been done to elucidate infectious virus secretion or serotype variability. Here, we used 51 respiratory samples from 32 patients with confirmed MERS-CoV infection for virus isolation in Vero B4 and Caco-2 cells. We found Caco-2 cells to significantly enhance isolation success over routinely used Vero cells. Isolation success correlated with viral RNA concentration and time after diagnosis as well as with the amount of IgA antibodies secreted in respiratory samples used for isolation. Results from plaque reduction neutralization assays using a representative range of serum samples and virus isolates suggested that all circulating human MERS-CoV strains represent one single serotype. The choice of prototype strain is not likely to influence the success of candidate MERS-CoV vaccines. However, vaccine formulations should be evaluated for their potential to induce IgA. </AbstractText>
<CopyrightInformation>Copyright © 2015, American Society for Microbiology. All Rights Reserved.</CopyrightInformation>
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<ForeName>Victor M</ForeName>
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<Initials>M</Initials>
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<Initials>M</Initials>
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<Initials>E</Initials>
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<ForeName>Jaffar A</ForeName>
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