A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus.
Identifieur interne : 001449 ( PubMed/Curation ); précédent : 001448; suivant : 001450A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus.
Auteurs : Yixin Chen [République populaire de Chine] ; Kwok-Hung Chan [République populaire de Chine] ; Yahong Kang [République populaire de Chine] ; Honglin Chen [République populaire de Chine] ; Hayes K H. Luk [République populaire de Chine] ; Rosana W S. Poon [République populaire de Chine] ; Jasper F W. Chan [République populaire de Chine] ; Kwok-Yung Yuen [République populaire de Chine] ; Ningshao Xia [République populaire de Chine] ; Susanna K P. Lau [République populaire de Chine] ; Patrick C Y. Woo [République populaire de Chine]Source :
- Emerging microbes & infections [ 2222-1751 ] ; 2015.
Descripteurs français
- KwdFr :
- Animaux, Anticorps monoclonaux (biosynthèse), Anticorps monoclonaux (immunologie), Antigènes viraux (analyse), Chameaux, Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Femelle, Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (immunologie), Protéines nucléocapside (analyse), Protéines nucléocapside (génétique), Protéines nucléocapside (métabolisme), Réservoirs d'agents pathogènes, Sensibilité et spécificité, Souris, Souris de lignée BALB C, Test ELISA (), Test ELISA (normes), Zoonoses (diagnostic), Zoonoses (immunologie).
- MESH :
- analyse : Antigènes viraux, Protéines nucléocapside.
- biosynthèse : Anticorps monoclonaux.
- diagnostic : Infections à coronavirus, Zoonoses.
- génétique : Protéines nucléocapside.
- immunologie : Anticorps monoclonaux, Coronavirus du syndrome respiratoire du Moyen-Orient, Infections à coronavirus, Zoonoses.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- métabolisme : Protéines nucléocapside.
- normes : Test ELISA.
- Animaux, Chameaux, Femelle, Humains, Réservoirs d'agents pathogènes, Sensibilité et spécificité, Souris, Souris de lignée BALB C, Test ELISA.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (biosynthesis), Antibodies, Monoclonal (immunology), Antigens, Viral (analysis), Camelus, Coronavirus Infections (diagnosis), Coronavirus Infections (immunology), Disease Reservoirs, Enzyme-Linked Immunosorbent Assay (methods), Enzyme-Linked Immunosorbent Assay (standards), Female, Humans, Mice, Mice, Inbred BALB C, Middle East Respiratory Syndrome Coronavirus (immunology), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Nucleocapsid Proteins (analysis), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (metabolism), Sensitivity and Specificity, Zoonoses (diagnosis), Zoonoses (immunology).
- MESH :
- chemical , analysis : Antigens, Viral, Nucleocapsid Proteins.
- chemical , biosynthesis : Antibodies, Monoclonal.
- chemical , genetics : Nucleocapsid Proteins.
- chemical , immunology : Antibodies, Monoclonal.
- diagnosis : Coronavirus Infections, Zoonoses.
- immunology : Coronavirus Infections, Middle East Respiratory Syndrome Coronavirus, Zoonoses.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- chemical , metabolism : Nucleocapsid Proteins.
- methods : Enzyme-Linked Immunosorbent Assay.
- standards : Enzyme-Linked Immunosorbent Assay.
- Animals, Camelus, Disease Reservoirs, Female, Humans, Mice, Mice, Inbred BALB C, Sensitivity and Specificity.
Abstract
Since its emergence in 2012, more than 900 laboratory-confirmed cases of Middle East respiratory syndrome (MERS) have been reported with a fatality rate of more than 30%. However, no antigen detection assay for commercial use is available for diagnosis. In this study, the full-length nucleocapsid protein (NP) gene of MERS coronavirus (MERS-CoV) was cloned and expressed in Escherichia coli. A MERS-CoV NP capture enzyme-linked immunosorbent assay (ELISA) using two MERS-CoV-NP-specific monoclonal antibodies (MAbs) generated was developed. The ELISA was evaluated using 129 nasopharyngeal aspirates (NPAs) positive for various respiratory viruses and simulated positive NPAs by adding serial dilutions of MERS-CoV. Using a cutoff OD of 0.19, all 129 NPAs positive for respiratory viruses showed very low OD, with a specificity of 100%. For the two simulated MERS-CoV-positive NPAs with serial dilutions of live MERS-CoV, all samples with ≥10 50% tissue culture infective dose (TCID50)/0.1 mL showed positive results. For the 10 additional NPAs with 20 and 200 TCID50/0.1 mL of live MERS-CoV added, all were positive. A highly sensitive and specific MAbs-based antigen capture ELISA has been developed for MERS. This sensitive and specific antigen capture ELISA should be useful for detection of MERS-CoV in human and dromedaries and in field studies.
DOI: 10.1038/emi.2015.26
PubMed: 26421268
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001449
Links to Exploration step
pubmed:26421268Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus.</title><author><name sortKey="Chen, Yixin" sort="Chen, Yixin" uniqKey="Chen Y" first="Yixin" last="Chen">Yixin Chen</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University , Xiamen 361102, Fujian Province, China ; National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University , Xiamen 361102, Fujian Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University , Xiamen 361102, Fujian Province, China ; National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University , Xiamen 361102, Fujian Province</wicri:regionArea></affiliation></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong , Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Kang, Yahong" sort="Kang, Yahong" uniqKey="Kang Y" first="Yahong" last="Kang">Yahong Kang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University , Xiamen 361102, Fujian Province, China ; National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University , Xiamen 361102, Fujian Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University , Xiamen 361102, Fujian Province, China ; National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University , Xiamen 361102, Fujian Province</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Honglin" sort="Chen, Honglin" uniqKey="Chen H" first="Honglin" last="Chen">Honglin Chen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Luk, Hayes K H" sort="Luk, Hayes K H" uniqKey="Luk H" first="Hayes K H" last="Luk">Hayes K H. Luk</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong , Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Poon, Rosana W S" sort="Poon, Rosana W S" uniqKey="Poon R" first="Rosana W S" last="Poon">Rosana W S. Poon</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong , Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Chan, Jasper F W" sort="Chan, Jasper F W" uniqKey="Chan J" first="Jasper F W" last="Chan">Jasper F W. Chan</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Xia, Ningshao" sort="Xia, Ningshao" uniqKey="Xia N" first="Ningshao" last="Xia">Ningshao Xia</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University , Xiamen 361102, Fujian Province, China ; National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University , Xiamen 361102, Fujian Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University , Xiamen 361102, Fujian Province, China ; National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University , Xiamen 361102, Fujian Province</wicri:regionArea></affiliation></author><author><name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K P" last="Lau">Susanna K P. Lau</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Woo, Patrick C Y" sort="Woo, Patrick C Y" uniqKey="Woo P" first="Patrick C Y" last="Woo">Patrick C Y. Woo</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26421268</idno><idno type="pmid">26421268</idno><idno type="doi">10.1038/emi.2015.26</idno><idno type="wicri:Area/PubMed/Corpus">001449</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001449</idno><idno type="wicri:Area/PubMed/Curation">001449</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001449</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus.</title><author><name sortKey="Chen, Yixin" sort="Chen, Yixin" uniqKey="Chen Y" first="Yixin" last="Chen">Yixin Chen</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University , Xiamen 361102, Fujian Province, China ; National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University , Xiamen 361102, Fujian Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University , Xiamen 361102, Fujian Province, China ; National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University , Xiamen 361102, Fujian Province</wicri:regionArea></affiliation></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong , Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Kang, Yahong" sort="Kang, Yahong" uniqKey="Kang Y" first="Yahong" last="Kang">Yahong Kang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University , Xiamen 361102, Fujian Province, China ; National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University , Xiamen 361102, Fujian Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University , Xiamen 361102, Fujian Province, China ; National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University , Xiamen 361102, Fujian Province</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Honglin" sort="Chen, Honglin" uniqKey="Chen H" first="Honglin" last="Chen">Honglin Chen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Luk, Hayes K H" sort="Luk, Hayes K H" uniqKey="Luk H" first="Hayes K H" last="Luk">Hayes K H. Luk</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong , Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Poon, Rosana W S" sort="Poon, Rosana W S" uniqKey="Poon R" first="Rosana W S" last="Poon">Rosana W S. Poon</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong , Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Chan, Jasper F W" sort="Chan, Jasper F W" uniqKey="Chan J" first="Jasper F W" last="Chan">Jasper F W. Chan</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Xia, Ningshao" sort="Xia, Ningshao" uniqKey="Xia N" first="Ningshao" last="Xia">Ningshao Xia</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University , Xiamen 361102, Fujian Province, China ; National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University , Xiamen 361102, Fujian Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University , Xiamen 361102, Fujian Province, China ; National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University , Xiamen 361102, Fujian Province</wicri:regionArea></affiliation></author><author><name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K P" last="Lau">Susanna K P. Lau</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Woo, Patrick C Y" sort="Woo, Patrick C Y" uniqKey="Woo P" first="Patrick C Y" last="Woo">Patrick C Y. Woo</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong</wicri:regionArea></affiliation></author></analytic><series><title level="j">Emerging microbes & infections</title><idno type="ISSN">2222-1751</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (biosynthesis)</term><term>Antibodies, Monoclonal (immunology)</term><term>Antigens, Viral (analysis)</term><term>Camelus</term><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (immunology)</term><term>Disease Reservoirs</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Enzyme-Linked Immunosorbent Assay (standards)</term><term>Female</term><term>Humans</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Middle East Respiratory Syndrome Coronavirus (immunology)</term><term>Middle East Respiratory Syndrome Coronavirus (isolation & purification)</term><term>Nucleocapsid Proteins (analysis)</term><term>Nucleocapsid Proteins (genetics)</term><term>Nucleocapsid Proteins (metabolism)</term><term>Sensitivity and Specificity</term><term>Zoonoses (diagnosis)</term><term>Zoonoses (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps monoclonaux (biosynthèse)</term><term>Anticorps monoclonaux (immunologie)</term><term>Antigènes viraux (analyse)</term><term>Chameaux</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term><term>Femelle</term><term>Humains</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (immunologie)</term><term>Protéines nucléocapside (analyse)</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (métabolisme)</term><term>Réservoirs d'agents pathogènes</term><term>Sensibilité et spécificité</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Test ELISA ()</term><term>Test ELISA (normes)</term><term>Zoonoses (diagnostic)</term><term>Zoonoses (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Viral</term><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Antigènes viraux</term><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anticorps monoclonaux</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term><term>Zoonoses</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term><term>Zoonoses</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps monoclonaux</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Infections à coronavirus</term><term>Zoonoses</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronavirus Infections</term><term>Middle East Respiratory Syndrome Coronavirus</term><term>Zoonoses</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" qualifier="normes" xml:lang="fr"><term>Test ELISA</term></keywords><keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Camelus</term><term>Disease Reservoirs</term><term>Female</term><term>Humans</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Chameaux</term><term>Femelle</term><term>Humains</term><term>Réservoirs d'agents pathogènes</term><term>Sensibilité et spécificité</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Since its emergence in 2012, more than 900 laboratory-confirmed cases of Middle East respiratory syndrome (MERS) have been reported with a fatality rate of more than 30%. However, no antigen detection assay for commercial use is available for diagnosis. In this study, the full-length nucleocapsid protein (NP) gene of MERS coronavirus (MERS-CoV) was cloned and expressed in Escherichia coli. A MERS-CoV NP capture enzyme-linked immunosorbent assay (ELISA) using two MERS-CoV-NP-specific monoclonal antibodies (MAbs) generated was developed. The ELISA was evaluated using 129 nasopharyngeal aspirates (NPAs) positive for various respiratory viruses and simulated positive NPAs by adding serial dilutions of MERS-CoV. Using a cutoff OD of 0.19, all 129 NPAs positive for respiratory viruses showed very low OD, with a specificity of 100%. For the two simulated MERS-CoV-positive NPAs with serial dilutions of live MERS-CoV, all samples with ≥10 50% tissue culture infective dose (TCID50)/0.1 mL showed positive results. For the 10 additional NPAs with 20 and 200 TCID50/0.1 mL of live MERS-CoV added, all were positive. A highly sensitive and specific MAbs-based antigen capture ELISA has been developed for MERS. This sensitive and specific antigen capture ELISA should be useful for detection of MERS-CoV in human and dromedaries and in field studies. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26421268</PMID><DateCompleted><Year>2016</Year><Month>05</Month><Day>23</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">2222-1751</ISSN><JournalIssue CitedMedium="Print"><Volume>4</Volume><Issue>4</Issue><PubDate><Year>2015</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Emerging microbes & infections</Title><ISOAbbreviation>Emerg Microbes Infect</ISOAbbreviation></Journal><ArticleTitle>A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus.</ArticleTitle><Pagination><MedlinePgn>e26</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/emi.2015.26</ELocationID><Abstract><AbstractText>Since its emergence in 2012, more than 900 laboratory-confirmed cases of Middle East respiratory syndrome (MERS) have been reported with a fatality rate of more than 30%. However, no antigen detection assay for commercial use is available for diagnosis. In this study, the full-length nucleocapsid protein (NP) gene of MERS coronavirus (MERS-CoV) was cloned and expressed in Escherichia coli. A MERS-CoV NP capture enzyme-linked immunosorbent assay (ELISA) using two MERS-CoV-NP-specific monoclonal antibodies (MAbs) generated was developed. The ELISA was evaluated using 129 nasopharyngeal aspirates (NPAs) positive for various respiratory viruses and simulated positive NPAs by adding serial dilutions of MERS-CoV. Using a cutoff OD of 0.19, all 129 NPAs positive for respiratory viruses showed very low OD, with a specificity of 100%. For the two simulated MERS-CoV-positive NPAs with serial dilutions of live MERS-CoV, all samples with ≥10 50% tissue culture infective dose (TCID50)/0.1 mL showed positive results. For the 10 additional NPAs with 20 and 200 TCID50/0.1 mL of live MERS-CoV added, all were positive. A highly sensitive and specific MAbs-based antigen capture ELISA has been developed for MERS. This sensitive and specific antigen capture ELISA should be useful for detection of MERS-CoV in human and dromedaries and in field studies. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Yixin</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University , Xiamen 361102, Fujian Province, China ; National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University , Xiamen 361102, Fujian Province, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chan</LastName><ForeName>Kwok-Hung</ForeName><Initials>KH</Initials><AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kang</LastName><ForeName>Yahong</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University , Xiamen 361102, Fujian Province, China ; National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University , Xiamen 361102, Fujian Province, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Honglin</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Luk</LastName><ForeName>Hayes K H</ForeName><Initials>HK</Initials><AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Poon</LastName><ForeName>Rosana W S</ForeName><Initials>RW</Initials><AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chan</LastName><ForeName>Jasper F W</ForeName><Initials>JF</Initials><AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yuen</LastName><ForeName>Kwok-Yung</ForeName><Initials>KY</Initials><AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xia</LastName><ForeName>Ningshao</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University , Xiamen 361102, Fujian Province, China ; National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University , Xiamen 361102, Fujian Province, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lau</LastName><ForeName>Susanna K P</ForeName><Initials>SK</Initials><AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Woo</LastName><ForeName>Patrick C Y</ForeName><Initials>PC</Initials><AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>04</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Emerg Microbes Infect</MedlineTA><NlmUniqueID>101594885</NlmUniqueID><ISSNLinking>2222-1751</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000956">Antigens, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000911" MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000956" MajorTopicYN="N">Antigens, Viral</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002162" MajorTopicYN="N">Camelus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004197" MajorTopicYN="N">Disease Reservoirs</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName><QualifierName UI="Q000592" MajorTopicYN="Y">standards</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D065207" MajorTopicYN="N">Middle East Respiratory Syndrome Coronavirus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019590" MajorTopicYN="N">Nucleocapsid Proteins</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015047" MajorTopicYN="N">Zoonoses</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">ELISA</Keyword><Keyword MajorTopicYN="N">MERS coronavirus</Keyword><Keyword MajorTopicYN="N">antigen</Keyword><Keyword MajorTopicYN="N">detection</Keyword></KeywordList><GeneralNote Owner="NLM">Original DateCompleted: 20150930</GeneralNote></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>02</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>03</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>03</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>10</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>10</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>10</Month><Day>1</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26421268</ArticleId><ArticleId IdType="doi">10.1038/emi.2015.26</ArticleId><ArticleId IdType="pmc">PMC4575394</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Emerg Infect Dis. 2014 Apr;20(4):560-72</Citation><ArticleIdList><ArticleId IdType="pubmed">24655427</ArticleId></ArticleIdList></Reference><Reference><Citation>J Med Virol. 2014 Jun;86(6):995-1002</Citation><ArticleIdList><ArticleId IdType="pubmed">24136709</ArticleId></ArticleIdList></Reference><Reference><Citation>MBio. 2014;5(3):e01146-14</Citation><ArticleIdList><ArticleId IdType="pubmed">24781747</ArticleId></ArticleIdList></Reference><Reference><Citation>Euro Surveill. 2014;19(16):20781</Citation><ArticleIdList><ArticleId IdType="pubmed">24786259</ArticleId></ArticleIdList></Reference><Reference><Citation>Emerg Infect Dis. 2014 Jun;20(6):1049-53</Citation><ArticleIdList><ArticleId IdType="pubmed">24856660</ArticleId></ArticleIdList></Reference><Reference><Citation>Emerg Infect Dis. 2014 Jul;20(7):1231-4</Citation><ArticleIdList><ArticleId IdType="pubmed">24964193</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2014 Jun 26;370(26):2499-505</Citation><ArticleIdList><ArticleId IdType="pubmed">24896817</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 2014 Sep;464-465:11-20</Citation><ArticleIdList><ArticleId IdType="pubmed">25043584</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2014 Oct;88(19):11297-303</Citation><ArticleIdList><ArticleId IdType="pubmed">25031349</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Host Microbe. 2014 Sep 10;16(3):328-37</Citation><ArticleIdList><ArticleId IdType="pubmed">25211075</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Infect Dis. 2014 Dec 1;59(11):1511-8</Citation><ArticleIdList><ArticleId IdType="pubmed">25100864</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Microbiol Rev. 2015 Apr;28(2):465-522</Citation><ArticleIdList><ArticleId IdType="pubmed">25810418</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2004 Jul;42(7):2884-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15243033</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 1997 Jun;35(6):1579-81</Citation><ArticleIdList><ArticleId IdType="pubmed">9163486</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2005 Jan;79(2):884-95</Citation><ArticleIdList><ArticleId IdType="pubmed">15613317</ArticleId></ArticleIdList></Reference><Reference><Citation>Emerg Infect Dis. 2005 Jul;11(7):1108-11</Citation><ArticleIdList><ArticleId IdType="pubmed">16022791</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2005 Sep;43(9):4515-21</Citation><ArticleIdList><ArticleId IdType="pubmed">16145100</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2006 Jun;44(6):2063-71</Citation><ArticleIdList><ArticleId IdType="pubmed">16757599</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2007 Nov;45(11):3655-64</Citation><ArticleIdList><ArticleId IdType="pubmed">17804649</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Microbiol Infect. 2011 Oct;17(10):1574-80</Citation><ArticleIdList><ArticleId IdType="pubmed">21054661</ArticleId></ArticleIdList></Reference><Reference><Citation>Euro Surveill. 2012;17(39). pii: 20285</Citation><ArticleIdList><ArticleId IdType="pubmed">23041020</ArticleId></ArticleIdList></Reference><Reference><Citation>Euro Surveill. 2012;17(40):20290</Citation><ArticleIdList><ArticleId IdType="pubmed">23078800</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2012 Nov 8;367(19):1814-20</Citation><ArticleIdList><ArticleId IdType="pubmed">23075143</ArticleId></ArticleIdList></Reference><Reference><Citation>MBio. 2012;3(6). pii: e00473-12. doi: 10.1128/mBio.00473-12</Citation><ArticleIdList><ArticleId IdType="pubmed">23170002</ArticleId></ArticleIdList></Reference><Reference><Citation>J Infect Dis. 2013 Jun 1;207(11):1743-52</Citation><ArticleIdList><ArticleId IdType="pubmed">23532101</ArticleId></ArticleIdList></Reference><Reference><Citation>J Infect. 2013 Aug;67(2):130-40</Citation><ArticleIdList><ArticleId IdType="pubmed">23583636</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2013 Jul;87(14):7790-2</Citation><ArticleIdList><ArticleId IdType="pubmed">23678167</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2013 Jun 29;381(9885):2265-72</Citation><ArticleIdList><ArticleId IdType="pubmed">23727167</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2013 Aug;87(15):8638-50</Citation><ArticleIdList><ArticleId IdType="pubmed">23720729</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet Infect Dis. 2013 Sep;13(9):745-51</Citation><ArticleIdList><ArticleId IdType="pubmed">23782859</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet Infect Dis. 2013 Oct;13(10):859-66</Citation><ArticleIdList><ArticleId IdType="pubmed">23933067</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 2013 Dec;94(Pt 12):2679-90</Citation><ArticleIdList><ArticleId IdType="pubmed">24077366</ArticleId></ArticleIdList></Reference><Reference><Citation>Euro Surveill. 2013;18(50):20662</Citation><ArticleIdList><ArticleId IdType="pubmed">24342516</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2014 Jan;52(1):67-75</Citation><ArticleIdList><ArticleId IdType="pubmed">24153118</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet Infect Dis. 2014 Feb;14(2):140-5</Citation><ArticleIdList><ArticleId IdType="pubmed">24355866</ArticleId></ArticleIdList></Reference><Reference><Citation>MBio. 2014;5(2):e00884-14</Citation><ArticleIdList><ArticleId IdType="pubmed">24570370</ArticleId></ArticleIdList></Reference><Reference><Citation>Emerg Infect Dis. 2014 Apr;20(4):552-9</Citation><ArticleIdList><ArticleId IdType="pubmed">24655412</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001449 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd -nk 001449 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= PubMed
|étape= Curation
|type= RBID
|clé= pubmed:26421268
|texte= A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Curation/RBID.i -Sk "pubmed:26421268" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |