MersV1, PubMed, Curation, bibRecord, 001276

Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak.

Identifieur interne : 001276 ( PubMed/Curation ); précédent : 001275; suivant : 001277

Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak.

Auteurs : Wan Beom Park [Corée du Sud] ; Nak-Jung Kwon [Corée du Sud] ; Pyoeng Gyun Choe [Corée du Sud] ; Su-Jin Choi [Corée du Sud] ; Hong Sang Oh [Corée du Sud] ; Sang Min Lee [Corée du Sud] ; Hyonyong Chong [Corée du Sud] ; Jong-Il Kim [Corée du Sud] ; Kyoung-Ho Song [Corée du Sud] ; Ji Hwan Bang [Corée du Sud] ; Eu Suk Kim [Corée du Sud] ; Hong-Bin Kim [Corée du Sud] ; Sang Won Park [Corée du Sud] ; Nam Joong Kim [Corée du Sud] ; Myoung-Don Oh [Corée du Sud]

Source :

Journal of Korean medical science [ 1598-6357 ] ; 2016.

RBID : pubmed:26839489

Descripteurs français

KwdFr :
- ARN viral (), ARN viral (analyse), ARN viral (métabolisme), Analyse de séquence d'ARN, Animaux, Cellules Vero, Coronavirus du syndrome respiratoire du Moyen-Orient (), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Coronavirus du syndrome respiratoire du Moyen-Orient (ultrastructure), Flambées de maladies, Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (virologie), Infections à coronavirus (épidémiologie), Microscopie électronique, Phylogénie, Réaction de polymérisation en chaîne, République de Corée (épidémiologie).
MESH :
- analyse : ARN viral.
- diagnostic : Infections à coronavirus.
- génétique : Coronavirus du syndrome respiratoire du Moyen-Orient.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- métabolisme : ARN viral.
- virologie : Infections à coronavirus.
- épidémiologie : Coronavirus du syndrome respiratoire du Moyen-Orient, Infections à coronavirus, République de Corée.
- ARN viral, Analyse de séquence d'ARN, Animaux, Cellules Vero, Coronavirus du syndrome respiratoire du Moyen-Orient, Flambées de maladies, Humains, Microscopie électronique, Phylogénie, Réaction de polymérisation en chaîne.

English descriptors

KwdEn :
- Animals, Chlorocebus aethiops, Coronavirus Infections (diagnosis), Coronavirus Infections (epidemiology), Coronavirus Infections (virology), Disease Outbreaks, Humans, Microscopy, Electron, Middle East Respiratory Syndrome Coronavirus (classification), Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Middle East Respiratory Syndrome Coronavirus (ultrastructure), Phylogeny, Polymerase Chain Reaction, RNA, Viral (analysis), RNA, Viral (chemistry), RNA, Viral (metabolism), Republic of Korea (epidemiology), Sequence Analysis, RNA, Vero Cells.
MESH :
- chemical , analysis : RNA, Viral.
- chemical , chemistry : RNA, Viral.
- geographic , epidemiology : Republic of Korea.
- classification : Middle East Respiratory Syndrome Coronavirus.
- diagnosis : Coronavirus Infections.
- epidemiology : Coronavirus Infections.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- chemical , metabolism : RNA, Viral.
- ultrastructure : Middle East Respiratory Syndrome Coronavirus.
- virology : Coronavirus Infections.
- Animals, Chlorocebus aethiops, Disease Outbreaks, Humans, Microscopy, Electron, Phylogeny, Polymerase Chain Reaction, Sequence Analysis, RNA, Vero Cells.

Abstract

During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV.

DOI: 10.3346/jkms.2016.31.2.315
PubMed: 26839489

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Le document en format XML

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<front><div type="abstract" xml:lang="en">During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV. </div>
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<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26839489</PMID>
<DateCompleted><Year>2016</Year>
<Month>10</Month>
<Day>28</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>12</Month>
<Day>10</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1598-6357</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>31</Volume>
<Issue>2</Issue>
<PubDate><Year>2016</Year>
<Month>Feb</Month>
</PubDate>
</JournalIssue>
<Title>Journal of Korean medical science</Title>
<ISOAbbreviation>J. Korean Med. Sci.</ISOAbbreviation>
</Journal>
<ArticleTitle>Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak.</ArticleTitle>
<Pagination><MedlinePgn>315-20</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.3346/jkms.2016.31.2.315</ELocationID>
<Abstract><AbstractText>During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV. </AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Park</LastName>
<ForeName>Wan Beom</ForeName>
<Initials>WB</Initials>
<Identifier Source="ORCID">0000-0003-0022-9625</Identifier>
<AffiliationInfo><Affiliation>Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.; Laboratory of Infection & Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kwon</LastName>
<ForeName>Nak-Jung</ForeName>
<Initials>NJ</Initials>
<Identifier Source="ORCID">0000-0003-0874-4146</Identifier>
<AffiliationInfo><Affiliation>Macrogen, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Choe</LastName>
<ForeName>Pyoeng Gyun</ForeName>
<Initials>PG</Initials>
<Identifier Source="ORCID">0000-0001-6794-7918</Identifier>
<AffiliationInfo><Affiliation>Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Choi</LastName>
<ForeName>Su-Jin</ForeName>
<Initials>SJ</Initials>
<Identifier Source="ORCID">0000-0001-8732-3950</Identifier>
<AffiliationInfo><Affiliation>Laboratory of Infection & Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Oh</LastName>
<ForeName>Hong Sang</ForeName>
<Initials>HS</Initials>
<Identifier Source="ORCID">0000-0002-4535-6305</Identifier>
<AffiliationInfo><Affiliation>Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lee</LastName>
<ForeName>Sang Min</ForeName>
<Initials>SM</Initials>
<Identifier Source="ORCID">0000-0002-1388-9318</Identifier>
<AffiliationInfo><Affiliation>Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chong</LastName>
<ForeName>Hyonyong</ForeName>
<Initials>H</Initials>
<Identifier Source="ORCID">0000-0001-6928-8805</Identifier>
<AffiliationInfo><Affiliation>Macrogen, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kim</LastName>
<ForeName>Jong-Il</ForeName>
<Initials>JI</Initials>
<Identifier Source="ORCID">0000-0002-7240-3744</Identifier>
<AffiliationInfo><Affiliation>Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Song</LastName>
<ForeName>Kyoung-Ho</ForeName>
<Initials>KH</Initials>
<Identifier Source="ORCID">0000-0002-4517-3840</Identifier>
<AffiliationInfo><Affiliation>Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Bang</LastName>
<ForeName>Ji Hwan</ForeName>
<Initials>JH</Initials>
<Identifier Source="ORCID">0000-0002-7628-1182</Identifier>
<AffiliationInfo><Affiliation>Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kim</LastName>
<ForeName>Eu Suk</ForeName>
<Initials>ES</Initials>
<Identifier Source="ORCID">0000-0001-7132-0157</Identifier>
<AffiliationInfo><Affiliation>Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kim</LastName>
<ForeName>Hong-Bin</ForeName>
<Initials>HB</Initials>
<Identifier Source="ORCID">0000-0001-6262-372X</Identifier>
<AffiliationInfo><Affiliation>Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Park</LastName>
<ForeName>Sang Won</ForeName>
<Initials>SW</Initials>
<Identifier Source="ORCID">0000-0002-0550-1897</Identifier>
<AffiliationInfo><Affiliation>Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kim</LastName>
<ForeName>Nam Joong</ForeName>
<Initials>NJ</Initials>
<Identifier Source="ORCID">0000-0001-6793-9467</Identifier>
<AffiliationInfo><Affiliation>Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.; Laboratory of Infection & Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Oh</LastName>
<ForeName>Myoung-Don</ForeName>
<Initials>MD</Initials>
<Identifier Source="ORCID">0000-0002-2344-7695</Identifier>
<AffiliationInfo><Affiliation>Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.; Laboratory of Infection & Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, Korea.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2016</Year>
<Month>01</Month>
<Day>14</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Korea (South)</Country>
<MedlineTA>J Korean Med Sci</MedlineTA>
<NlmUniqueID>8703518</NlmUniqueID>
<ISSNLinking>1011-8934</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName>
<QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName>
<QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004196" MajorTopicYN="N">Disease Outbreaks</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008854" MajorTopicYN="N">Microscopy, Electron</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D065207" MajorTopicYN="N">Middle East Respiratory Syndrome Coronavirus</DescriptorName>
<QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
<QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D056910" MajorTopicYN="N" Type="Geographic">Republic of Korea</DescriptorName>
<QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017423" MajorTopicYN="N">Sequence Analysis, RNA</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Korea</Keyword>
<Keyword MajorTopicYN="N">Microscopy, Electron</Keyword>
<Keyword MajorTopicYN="N">Middle East Respiratory Syndrome</Keyword>
<Keyword MajorTopicYN="N">Middle East Respiratory Syndrome Coronavirus</Keyword>
<Keyword MajorTopicYN="N">Phylogeny</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year>
<Month>12</Month>
<Day>29</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2016</Year>
<Month>01</Month>
<Day>11</Day>
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Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak.

Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak.

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