MERS-CoV diagnosis: An update.
Identifieur interne : 001153 ( PubMed/Curation ); précédent : 001152; suivant : 001154MERS-CoV diagnosis: An update.
Auteurs : Sameera Al Johani [Arabie saoudite] ; Ali H. Hajeer [Arabie saoudite]Source :
- Journal of infection and public health [ 1876-035X ]
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN (), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Humains, Infections à coronavirus (diagnostic), Réaction de polymérisation en chaine en temps réel (), Techniques de diagnostic moléculaire (), Tests sérologiques ().
- MESH :
- diagnostic : Infections à coronavirus.
- génétique : Coronavirus du syndrome respiratoire du Moyen-Orient.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- Analyse de séquence d'ADN, Humains, Réaction de polymérisation en chaine en temps réel, Techniques de diagnostic moléculaire, Tests sérologiques.
English descriptors
- KwdEn :
- Coronavirus Infections (diagnosis), Humans, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Molecular Diagnostic Techniques (methods), Real-Time Polymerase Chain Reaction (methods), Sequence Analysis, DNA (methods), Serologic Tests (methods).
- MESH :
- diagnosis : Coronavirus Infections.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- methods : Molecular Diagnostic Techniques, Real-Time Polymerase Chain Reaction, Sequence Analysis, DNA, Serologic Tests.
- Humans.
Abstract
Diagnosis of MERS-Cov still a major concern in most of daignostic laboratories. To date the Real-time Polymerase Chain reaction (RT-PCR) is the mainstay for diagnosis of MERS-CoV. RT-PCR has limitations, including a long turnaround time and lack of common measurements and correlations with Viral Load (VL). It is recommended to screen for MERS-CoV using RT-PCR of the upstream of envelope gene (upE) followed by confirmation of the presence of one of the following genes; open reading frame 1A, 1B genes or nucleocapsid (N) gene. Scientists are looking to implement viral sequencing on all negative samples by RT-PCR and they beleive that can be exposed to another level of testing using sequencing of the RNA-dependent RNA polymerase (RdRp) gene or N gene and in this case a positive result is diagnostic. It is also very important to maintain a contineous and random sequencing for MERS-Cov samples to be able to pick early viral mutations. Serological assays still not widely or routinely performed, and a lot of studies looking to implement such method in routine patient's testings.
DOI: 10.1016/j.jiph.2016.04.005
PubMed: 27106390
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<term>Humains</term>
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<front><div type="abstract" xml:lang="en">Diagnosis of MERS-Cov still a major concern in most of daignostic laboratories. To date the Real-time Polymerase Chain reaction (RT-PCR) is the mainstay for diagnosis of MERS-CoV. RT-PCR has limitations, including a long turnaround time and lack of common measurements and correlations with Viral Load (VL). It is recommended to screen for MERS-CoV using RT-PCR of the upstream of envelope gene (upE) followed by confirmation of the presence of one of the following genes; open reading frame 1A, 1B genes or nucleocapsid (N) gene. Scientists are looking to implement viral sequencing on all negative samples by RT-PCR and they beleive that can be exposed to another level of testing using sequencing of the RNA-dependent RNA polymerase (RdRp) gene or N gene and in this case a positive result is diagnostic. It is also very important to maintain a contineous and random sequencing for MERS-Cov samples to be able to pick early viral mutations. Serological assays still not widely or routinely performed, and a lot of studies looking to implement such method in routine patient's testings. </div>
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