Development of Dual TaqMan Based One-Step rRT-PCR Assay Panel for Rapid and Accurate Diagnostic Test of MERS-CoV: A Novel Human Coronavirus, Ahead of Hajj Pilgrimage.
Identifieur interne : 000D83 ( PubMed/Curation ); précédent : 000D82; suivant : 000D84Development of Dual TaqMan Based One-Step rRT-PCR Assay Panel for Rapid and Accurate Diagnostic Test of MERS-CoV: A Novel Human Coronavirus, Ahead of Hajj Pilgrimage.
Auteurs : Mohammad Sadegh Hashemzadeh ; Rahimeh Rasouli ; Bentolhoda Zahraei ; Morteza Izadi ; Mahdi Tat ; Seyed Hassan Saadat ; Mohammad Najarasl ; Behzad Khansari Nejad ; Ruhollah DorostkarSource :
- Iranian Red Crescent medical journal [ 2074-1804 ] ; 2016.
Abstract
Coronaviruses (CoVs) are large ribonucleic acid (RNA) viruses causing primarily respiratory disease in humans. A novel human coronavirus, subsequently named middle east respiratory syndrome coronavirus (MERS-CoV), was first reported in Saudi Arabia in September of 2012. With increasing numbers of infections and deaths from MERS-CoV, development of a rapid and reliable kit was crucial to prevent further spread of MERS-CoV.
DOI: 10.5812/ircmj.23874
PubMed: 28191331
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Mohammad Sadegh Hashemzadeh<affiliation><nlm:affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation><affiliation><nlm:affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation><affiliation><nlm:affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation><affiliation><nlm:affiliation>Health Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation><affiliation><nlm:affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation><affiliation><nlm:affiliation>Health Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation><affiliation><nlm:affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation><affiliation><nlm:affiliation>Department of Microbiology and Immunology, Arak University of Medical Sciences, Arak, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation><affiliation><nlm:affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation>Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development of Dual TaqMan Based One-Step rRT-PCR Assay Panel for Rapid and Accurate Diagnostic Test of MERS-CoV: A Novel Human Coronavirus, Ahead of Hajj Pilgrimage.</title><author><name sortKey="Hashemzadeh, Mohammad Sadegh" sort="Hashemzadeh, Mohammad Sadegh" uniqKey="Hashemzadeh M" first="Mohammad Sadegh" last="Hashemzadeh">Mohammad Sadegh Hashemzadeh</name><affiliation><nlm:affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Rasouli, Rahimeh" sort="Rasouli, Rahimeh" uniqKey="Rasouli R" first="Rahimeh" last="Rasouli">Rahimeh Rasouli</name><affiliation><nlm:affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Zahraei, Bentolhoda" sort="Zahraei, Bentolhoda" uniqKey="Zahraei B" first="Bentolhoda" last="Zahraei">Bentolhoda Zahraei</name><affiliation><nlm:affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Izadi, Morteza" sort="Izadi, Morteza" uniqKey="Izadi M" first="Morteza" last="Izadi">Morteza Izadi</name><affiliation><nlm:affiliation>Health Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Tat, Mahdi" sort="Tat, Mahdi" uniqKey="Tat M" first="Mahdi" last="Tat">Mahdi Tat</name><affiliation><nlm:affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Saadat, Seyed Hassan" sort="Saadat, Seyed Hassan" uniqKey="Saadat S" first="Seyed Hassan" last="Saadat">Seyed Hassan Saadat</name><affiliation><nlm:affiliation>Health Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Najarasl, Mohammad" sort="Najarasl, Mohammad" uniqKey="Najarasl M" first="Mohammad" last="Najarasl">Mohammad Najarasl</name><affiliation><nlm:affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Khansari Nejad, Behzad" sort="Khansari Nejad, Behzad" uniqKey="Khansari Nejad B" first="Behzad" last="Khansari Nejad">Behzad Khansari Nejad</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, Arak University of Medical Sciences, Arak, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Dorostkar, Ruhollah" sort="Dorostkar, Ruhollah" uniqKey="Dorostkar R" first="Ruhollah" last="Dorostkar">Ruhollah Dorostkar</name><affiliation><nlm:affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:28191331</idno><idno type="pmid">28191331</idno><idno type="doi">10.5812/ircmj.23874</idno><idno type="wicri:Area/PubMed/Corpus">000D83</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000D83</idno><idno type="wicri:Area/PubMed/Curation">000D83</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000D83</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Development of Dual TaqMan Based One-Step rRT-PCR Assay Panel for Rapid and Accurate Diagnostic Test of MERS-CoV: A Novel Human Coronavirus, Ahead of Hajj Pilgrimage.</title><author><name sortKey="Hashemzadeh, Mohammad Sadegh" sort="Hashemzadeh, Mohammad Sadegh" uniqKey="Hashemzadeh M" first="Mohammad Sadegh" last="Hashemzadeh">Mohammad Sadegh Hashemzadeh</name><affiliation><nlm:affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Rasouli, Rahimeh" sort="Rasouli, Rahimeh" uniqKey="Rasouli R" first="Rahimeh" last="Rasouli">Rahimeh Rasouli</name><affiliation><nlm:affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Zahraei, Bentolhoda" sort="Zahraei, Bentolhoda" uniqKey="Zahraei B" first="Bentolhoda" last="Zahraei">Bentolhoda Zahraei</name><affiliation><nlm:affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Izadi, Morteza" sort="Izadi, Morteza" uniqKey="Izadi M" first="Morteza" last="Izadi">Morteza Izadi</name><affiliation><nlm:affiliation>Health Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Tat, Mahdi" sort="Tat, Mahdi" uniqKey="Tat M" first="Mahdi" last="Tat">Mahdi Tat</name><affiliation><nlm:affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Saadat, Seyed Hassan" sort="Saadat, Seyed Hassan" uniqKey="Saadat S" first="Seyed Hassan" last="Saadat">Seyed Hassan Saadat</name><affiliation><nlm:affiliation>Health Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Najarasl, Mohammad" sort="Najarasl, Mohammad" uniqKey="Najarasl M" first="Mohammad" last="Najarasl">Mohammad Najarasl</name><affiliation><nlm:affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Khansari Nejad, Behzad" sort="Khansari Nejad, Behzad" uniqKey="Khansari Nejad B" first="Behzad" last="Khansari Nejad">Behzad Khansari Nejad</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, Arak University of Medical Sciences, Arak, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author><author><name sortKey="Dorostkar, Ruhollah" sort="Dorostkar, Ruhollah" uniqKey="Dorostkar R" first="Ruhollah" last="Dorostkar">Ruhollah Dorostkar</name><affiliation><nlm:affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</nlm:affiliation><wicri:noCountry code="subField">IR Iran</wicri:noCountry></affiliation></author></analytic><series><title level="j">Iranian Red Crescent medical journal</title><idno type="ISSN">2074-1804</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Coronaviruses (CoVs) are large ribonucleic acid (RNA) viruses causing primarily respiratory disease in humans. A novel human coronavirus, subsequently named middle east respiratory syndrome coronavirus (MERS-CoV), was first reported in Saudi Arabia in September of 2012. With increasing numbers of infections and deaths from MERS-CoV, development of a rapid and reliable kit was crucial to prevent further spread of MERS-CoV.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">28191331</PMID><DateRevised><Year>2019</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">2074-1804</ISSN><JournalIssue CitedMedium="Print"><Volume>18</Volume><Issue>11</Issue><PubDate><Year>2016</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Iranian Red Crescent medical journal</Title><ISOAbbreviation>Iran Red Crescent Med J</ISOAbbreviation></Journal><ArticleTitle>Development of Dual TaqMan Based One-Step rRT-PCR Assay Panel for Rapid and Accurate Diagnostic Test of MERS-CoV: A Novel Human Coronavirus, Ahead of Hajj Pilgrimage.</ArticleTitle><Pagination><MedlinePgn>e23874</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.5812/ircmj.23874</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Coronaviruses (CoVs) are large ribonucleic acid (RNA) viruses causing primarily respiratory disease in humans. A novel human coronavirus, subsequently named middle east respiratory syndrome coronavirus (MERS-CoV), was first reported in Saudi Arabia in September of 2012. With increasing numbers of infections and deaths from MERS-CoV, development of a rapid and reliable kit was crucial to prevent further spread of MERS-CoV.</AbstractText><AbstractText Label="OBJECTIVES" NlmCategory="OBJECTIVE">In this study, we present two real-time reverse-transcription polymerase chain reaction (rRT-PCR) assays for in-house rapid and sensitive diagnostic testing of MERS-CoV, detecting the regions upstream of the envelope gene (upE) and open reading frame (ORF) 1b, respectively, for initial screening and final confirmation of MERS-CoV infection, as recommended by the world health organization (WHO).</AbstractText><AbstractText Label="MATERIALS AND METHODS" NlmCategory="METHODS">In this experimental study, acquiring patient samples was difficult; thus, according to WHO recommendations and standard protocols, we synthesized RNA sequences of upE and ORF1b genes as the template signatures and TaqMan based-diagnostic rRT-PCR assays were carried out using these synthetic genes for detection of MERS-CoV. In this research, we also inaugurated a cell-free system to transcribe these RNA sequences using the DNA templates synthesized.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The upE and ORF1b based one-step rRT-PCR assays were optimized by testing several times via different synthetic RNAs, and validation results were highly successful. The sensitivity obtained for upE was fewer than ten copies of RNA template per reaction and for ORF1b was 50 or fewer copies per reaction.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">This study showed that the developed rRT-PCR assays are rapid, reliable, reproducible, specific, sensitive, and simple tools for detection of MERS-CoV. Finally, a kit consisting of two assay signatures and controls was assembled, which can be distributed to public health laboratories in Iran to support international MERS-CoV surveillance and public health response.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hashemzadeh</LastName><ForeName>Mohammad Sadegh</ForeName><Initials>MS</Initials><AffiliationInfo><Affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rasouli</LastName><ForeName>Rahimeh</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zahraei</LastName><ForeName>Bentolhoda</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Izadi</LastName><ForeName>Morteza</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Health Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tat</LastName><ForeName>Mahdi</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Saadat</LastName><ForeName>Seyed Hassan</ForeName><Initials>SH</Initials><AffiliationInfo><Affiliation>Health Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Najarasl</LastName><ForeName>Mohammad</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Khansari Nejad</LastName><ForeName>Behzad</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, Arak University of Medical Sciences, Arak, IR Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dorostkar</LastName><ForeName>Ruhollah</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>06</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>Iran</Country><MedlineTA>Iran Red Crescent Med J</MedlineTA><NlmUniqueID>101319850</NlmUniqueID><ISSNLinking>2074-1804</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Diagnosis</Keyword><Keyword MajorTopicYN="N">Hajj Pilgrimage</Keyword><Keyword MajorTopicYN="N">MERS-CoV</Keyword><Keyword MajorTopicYN="N">ORF1b</Keyword><Keyword MajorTopicYN="N">Real-Time RT-PCR</Keyword><Keyword MajorTopicYN="N">upE</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>09</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>11</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>04</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>2</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>2</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>2</Month><Day>14</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28191331</ArticleId><ArticleId IdType="doi">10.5812/ircmj.23874</ArticleId><ArticleId IdType="pmc">PMC5292137</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Euro Surveill. 2012 Sep 27;17(39):null</Citation><ArticleIdList><ArticleId IdType="pubmed">23041020</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2012 Nov 8;367 (19):1814-20</Citation><ArticleIdList><ArticleId IdType="pubmed">23075143</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2013 Jun 29;381(9885):2265-72</Citation><ArticleIdList><ArticleId IdType="pubmed">23727167</ArticleId></ArticleIdList></Reference><Reference><Citation>Antiviral Res. 2004 Dec;64(3):145-60</Citation><ArticleIdList><ArticleId IdType="pubmed">15550268</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2013 Jun 27;368(26):2487-94</Citation><ArticleIdList><ArticleId IdType="pubmed">23718156</ArticleId></ArticleIdList></Reference><Reference><Citation>Euro Surveill. 2013 Feb 21;18(8):null</Citation><ArticleIdList><ArticleId IdType="pubmed">23449231</ArticleId></ArticleIdList></Reference><Reference><Citation>Euro Surveill. 2013 Jun 13;18(24):null</Citation><ArticleIdList><ArticleId IdType="pubmed">23787161</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2013 Nov 20;8(11):e80028</Citation><ArticleIdList><ArticleId IdType="pubmed">24278234</ArticleId></ArticleIdList></Reference><Reference><Citation>Saudi Med J. 2013 Oct;34(10):991-4</Citation><ArticleIdList><ArticleId IdType="pubmed">24145930</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2013 Jul;87(14):7790-2</Citation><ArticleIdList><ArticleId IdType="pubmed">23678167</ArticleId></ArticleIdList></Reference><Reference><Citation>Oman Med J. 2013 Jul;28(4):226-7</Citation><ArticleIdList><ArticleId IdType="pubmed">23904912</ArticleId></ArticleIdList></Reference><Reference><Citation>MMWR Morb Mortal Wkly Rep. 2012 Oct 12;61(40):820</Citation><ArticleIdList><ArticleId IdType="pubmed">23051613</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Curr. 2013 Dec 12;5:null</Citation><ArticleIdList><ArticleId IdType="pubmed">24459611</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet Infect Dis. 2013 Sep;13(9):752-61</Citation><ArticleIdList><ArticleId IdType="pubmed">23891402</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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