6S RNA in Rhodobacter sphaeroides: 6S RNA and pRNA transcript levels peak in late exponential phase and gene deletion causes a high salt stress phenotype.
Identifieur interne : 000C30 ( PubMed/Curation ); précédent : 000C29; suivant : 000C316S RNA in Rhodobacter sphaeroides: 6S RNA and pRNA transcript levels peak in late exponential phase and gene deletion causes a high salt stress phenotype.
Auteurs : Daria Elkina [Russie] ; Lennart Weber [Allemagne] ; Marcus Lechner [Russie] ; Olga Burenina [Russie] ; Andrea Weisert [Allemagne] ; Elena Kubareva [Russie] ; Roland K. Hartmann [Russie] ; Gabriele Klug [Allemagne]Source :
- RNA biology [ 1555-8584 ] ; 2017.
Descripteurs français
- KwdFr :
- ARN bactérien (génétique), ARN bactérien (métabolisme), ARN messager (génétique), ARN messager (métabolisme), ARN non traduit (génétique), ARN non traduit (métabolisme), Adhésines bactériennes (génétique), Adhésines bactériennes (métabolisme), Chlorure de sodium (pharmacologie), DNA-directed RNA polymerases (génétique), DNA-directed RNA polymerases (métabolisme), Délétion de gène, Phénotype, Rhodobacter sphaeroides (), Rhodobacter sphaeroides (croissance et développement), Rhodobacter sphaeroides (génétique), Rhodobacter sphaeroides (métabolisme), Régulation de l'expression des gènes bactériens, Stress physiologique, Transcription génétique.
- MESH :
- croissance et développement : Rhodobacter sphaeroides.
- génétique : ARN bactérien, ARN messager, ARN non traduit, Adhésines bactériennes, DNA-directed RNA polymerases, Rhodobacter sphaeroides.
- métabolisme : ARN bactérien, ARN messager, ARN non traduit, Adhésines bactériennes, DNA-directed RNA polymerases, Rhodobacter sphaeroides.
- pharmacologie : Chlorure de sodium.
- Délétion de gène, Phénotype, Rhodobacter sphaeroides, Régulation de l'expression des gènes bactériens, Stress physiologique, Transcription génétique.
English descriptors
- KwdEn :
- Adhesins, Bacterial (genetics), Adhesins, Bacterial (metabolism), DNA-Directed RNA Polymerases (genetics), DNA-Directed RNA Polymerases (metabolism), Gene Deletion, Gene Expression Regulation, Bacterial, Phenotype, RNA, Bacterial (genetics), RNA, Bacterial (metabolism), RNA, Messenger (genetics), RNA, Messenger (metabolism), RNA, Untranslated (genetics), RNA, Untranslated (metabolism), Rhodobacter sphaeroides (drug effects), Rhodobacter sphaeroides (genetics), Rhodobacter sphaeroides (growth & development), Rhodobacter sphaeroides (metabolism), Sodium Chloride (pharmacology), Stress, Physiological, Transcription, Genetic.
- MESH :
- chemical , genetics : Adhesins, Bacterial, DNA-Directed RNA Polymerases, RNA, Bacterial, RNA, Messenger, RNA, Untranslated.
- chemical , metabolism : Adhesins, Bacterial, DNA-Directed RNA Polymerases, RNA, Bacterial, RNA, Messenger, RNA, Untranslated.
- drug effects : Rhodobacter sphaeroides.
- genetics : Rhodobacter sphaeroides.
- growth & development : Rhodobacter sphaeroides.
- metabolism : Rhodobacter sphaeroides.
- chemical , pharmacology : Sodium Chloride.
- Gene Deletion, Gene Expression Regulation, Bacterial, Phenotype, Stress, Physiological, Transcription, Genetic.
Abstract
The function of 6S RNA, a global regulator of transcription, was studied in the photosynthetic α-proteobacterium Rhodobacter sphaeroides. The cellular levels of R. sphaeroides 6S RNA peak toward the transition to stationary phase and strongly decrease during extended stationary phase. The synthesis of so-called product RNA transcripts (mainly 12-16-mers) on 6S RNA as template by RNA polymerase was found to be highest in late exponential phase. Product RNA ≥ 13-mers are expected to trigger the dissociation of 6S RNA:RNA polymerase complexes. A 6S RNA deletion in R. sphaeroides had no impact on growth under various metabolic and oxidative stress conditions (with the possible exception of tert-butyl hydroperoxide stress). However, the 6S RNA knockout resulted in a robust growth defect under high salt stress (0.25 M NaCl). Remarkably, the sspA gene encoding the putative salt stress-induced membrane protein SspA and located immediately downstream of the 6S RNA (ssrS) gene on the antisense strand was expressed at elevated levels in the ΔssrS strain when grown in the presence of 250 mM NaCl.
DOI: 10.1080/15476286.2017.1342933
PubMed: 28692405
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<series><title level="j">RNA biology</title>
<idno type="eISSN">1555-8584</idno>
<imprint><date when="2017" type="published">2017</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adhesins, Bacterial (genetics)</term>
<term>Adhesins, Bacterial (metabolism)</term>
<term>DNA-Directed RNA Polymerases (genetics)</term>
<term>DNA-Directed RNA Polymerases (metabolism)</term>
<term>Gene Deletion</term>
<term>Gene Expression Regulation, Bacterial</term>
<term>Phenotype</term>
<term>RNA, Bacterial (genetics)</term>
<term>RNA, Bacterial (metabolism)</term>
<term>RNA, Messenger (genetics)</term>
<term>RNA, Messenger (metabolism)</term>
<term>RNA, Untranslated (genetics)</term>
<term>RNA, Untranslated (metabolism)</term>
<term>Rhodobacter sphaeroides (drug effects)</term>
<term>Rhodobacter sphaeroides (genetics)</term>
<term>Rhodobacter sphaeroides (growth & development)</term>
<term>Rhodobacter sphaeroides (metabolism)</term>
<term>Sodium Chloride (pharmacology)</term>
<term>Stress, Physiological</term>
<term>Transcription, Genetic</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN bactérien (génétique)</term>
<term>ARN bactérien (métabolisme)</term>
<term>ARN messager (génétique)</term>
<term>ARN messager (métabolisme)</term>
<term>ARN non traduit (génétique)</term>
<term>ARN non traduit (métabolisme)</term>
<term>Adhésines bactériennes (génétique)</term>
<term>Adhésines bactériennes (métabolisme)</term>
<term>Chlorure de sodium (pharmacologie)</term>
<term>DNA-directed RNA polymerases (génétique)</term>
<term>DNA-directed RNA polymerases (métabolisme)</term>
<term>Délétion de gène</term>
<term>Phénotype</term>
<term>Rhodobacter sphaeroides ()</term>
<term>Rhodobacter sphaeroides (croissance et développement)</term>
<term>Rhodobacter sphaeroides (génétique)</term>
<term>Rhodobacter sphaeroides (métabolisme)</term>
<term>Régulation de l'expression des gènes bactériens</term>
<term>Stress physiologique</term>
<term>Transcription génétique</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Adhesins, Bacterial</term>
<term>DNA-Directed RNA Polymerases</term>
<term>RNA, Bacterial</term>
<term>RNA, Messenger</term>
<term>RNA, Untranslated</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Adhesins, Bacterial</term>
<term>DNA-Directed RNA Polymerases</term>
<term>RNA, Bacterial</term>
<term>RNA, Messenger</term>
<term>RNA, Untranslated</term>
</keywords>
<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Rhodobacter sphaeroides</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Rhodobacter sphaeroides</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Rhodobacter sphaeroides</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Rhodobacter sphaeroides</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN bactérien</term>
<term>ARN messager</term>
<term>ARN non traduit</term>
<term>Adhésines bactériennes</term>
<term>DNA-directed RNA polymerases</term>
<term>Rhodobacter sphaeroides</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Rhodobacter sphaeroides</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN bactérien</term>
<term>ARN messager</term>
<term>ARN non traduit</term>
<term>Adhésines bactériennes</term>
<term>DNA-directed RNA polymerases</term>
<term>Rhodobacter sphaeroides</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Chlorure de sodium</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Sodium Chloride</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Deletion</term>
<term>Gene Expression Regulation, Bacterial</term>
<term>Phenotype</term>
<term>Stress, Physiological</term>
<term>Transcription, Genetic</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Délétion de gène</term>
<term>Phénotype</term>
<term>Rhodobacter sphaeroides</term>
<term>Régulation de l'expression des gènes bactériens</term>
<term>Stress physiologique</term>
<term>Transcription génétique</term>
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<front><div type="abstract" xml:lang="en">The function of 6S RNA, a global regulator of transcription, was studied in the photosynthetic α-proteobacterium Rhodobacter sphaeroides. The cellular levels of R. sphaeroides 6S RNA peak toward the transition to stationary phase and strongly decrease during extended stationary phase. The synthesis of so-called product RNA transcripts (mainly 12-16-mers) on 6S RNA as template by RNA polymerase was found to be highest in late exponential phase. Product RNA ≥ 13-mers are expected to trigger the dissociation of 6S RNA:RNA polymerase complexes. A 6S RNA deletion in R. sphaeroides had no impact on growth under various metabolic and oxidative stress conditions (with the possible exception of tert-butyl hydroperoxide stress). However, the 6S RNA knockout resulted in a robust growth defect under high salt stress (0.25 M NaCl). Remarkably, the sspA gene encoding the putative salt stress-induced membrane protein SspA and located immediately downstream of the 6S RNA (ssrS) gene on the antisense strand was expressed at elevated levels in the ΔssrS strain when grown in the presence of 250 mM NaCl.</div>
</front>
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<DateCompleted><Year>2018</Year>
<Month>07</Month>
<Day>19</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
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<Issue>11</Issue>
<PubDate><Year>2017</Year>
<Month>11</Month>
<Day>02</Day>
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<Title>RNA biology</Title>
<ISOAbbreviation>RNA Biol</ISOAbbreviation>
</Journal>
<ArticleTitle>6S RNA in Rhodobacter sphaeroides: 6S RNA and pRNA transcript levels peak in late exponential phase and gene deletion causes a high salt stress phenotype.</ArticleTitle>
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</Pagination>
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<Abstract><AbstractText>The function of 6S RNA, a global regulator of transcription, was studied in the photosynthetic α-proteobacterium Rhodobacter sphaeroides. The cellular levels of R. sphaeroides 6S RNA peak toward the transition to stationary phase and strongly decrease during extended stationary phase. The synthesis of so-called product RNA transcripts (mainly 12-16-mers) on 6S RNA as template by RNA polymerase was found to be highest in late exponential phase. Product RNA ≥ 13-mers are expected to trigger the dissociation of 6S RNA:RNA polymerase complexes. A 6S RNA deletion in R. sphaeroides had no impact on growth under various metabolic and oxidative stress conditions (with the possible exception of tert-butyl hydroperoxide stress). However, the 6S RNA knockout resulted in a robust growth defect under high salt stress (0.25 M NaCl). Remarkably, the sspA gene encoding the putative salt stress-induced membrane protein SspA and located immediately downstream of the 6S RNA (ssrS) gene on the antisense strand was expressed at elevated levels in the ΔssrS strain when grown in the presence of 250 mM NaCl.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Elkina</LastName>
<ForeName>Daria</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>a Chemistry Department and A.N. Belozersky Institute of Physico-Chemical Biology , M.V. Lomonosov Moscow State University , Leninskie Gory 1, Moscow , Russia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Weber</LastName>
<ForeName>Lennart</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>b Institut für Mikrobiologie und Molekularbiologie, Justus-Liebig-University-Gießen, Heinrich-Buff-Ring 26-32 , Gießen , Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lechner</LastName>
<ForeName>Marcus</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>c Institut für Pharmazeutische Chemie, Philipps-Universität Marburg, Marbacher Weg 6 , Marburg , Germany ; Skolkovo Institute for Science and Technology , Skoltech, Moscow.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Burenina</LastName>
<ForeName>Olga</ForeName>
<Initials>O</Initials>
<AffiliationInfo><Affiliation>a Chemistry Department and A.N. Belozersky Institute of Physico-Chemical Biology , M.V. Lomonosov Moscow State University , Leninskie Gory 1, Moscow , Russia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Weisert</LastName>
<ForeName>Andrea</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>b Institut für Mikrobiologie und Molekularbiologie, Justus-Liebig-University-Gießen, Heinrich-Buff-Ring 26-32 , Gießen , Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kubareva</LastName>
<ForeName>Elena</ForeName>
<Initials>E</Initials>
<AffiliationInfo><Affiliation>a Chemistry Department and A.N. Belozersky Institute of Physico-Chemical Biology , M.V. Lomonosov Moscow State University , Leninskie Gory 1, Moscow , Russia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hartmann</LastName>
<ForeName>Roland K</ForeName>
<Initials>RK</Initials>
<AffiliationInfo><Affiliation>c Institut für Pharmazeutische Chemie, Philipps-Universität Marburg, Marbacher Weg 6 , Marburg , Germany ; Skolkovo Institute for Science and Technology , Skoltech, Moscow.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Klug</LastName>
<ForeName>Gabriele</ForeName>
<Initials>G</Initials>
<AffiliationInfo><Affiliation>b Institut für Mikrobiologie und Molekularbiologie, Justus-Liebig-University-Gießen, Heinrich-Buff-Ring 26-32 , Gießen , Germany.</Affiliation>
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<Language>eng</Language>
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<Month>09</Month>
<Day>13</Day>
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<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
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<MeshHeading><DescriptorName UI="D012242" MajorTopicYN="N">Rhodobacter sphaeroides</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
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<Keyword MajorTopicYN="Y">6S RNA</Keyword>
<Keyword MajorTopicYN="Y">Northern blot analysis</Keyword>
<Keyword MajorTopicYN="Y">RNA-Seq</Keyword>
<Keyword MajorTopicYN="Y">high salt stress</Keyword>
<Keyword MajorTopicYN="Y">pRNA synthesis</Keyword>
<Keyword MajorTopicYN="Y">ssrS deletion</Keyword>
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