A multiplex liquid-chip assay based on Luminex xMAP technology for simultaneous detection of six common respiratory viruses.
Identifieur interne : 000A56 ( PubMed/Curation ); précédent : 000A55; suivant : 000A57A multiplex liquid-chip assay based on Luminex xMAP technology for simultaneous detection of six common respiratory viruses.
Auteurs : Yong Yan [République populaire de Chine] ; Jian-Yong Luo [République populaire de Chine] ; Yin Chen [République populaire de Chine] ; Heng-Hui Wang [République populaire de Chine] ; Guo-Ying Zhu [République populaire de Chine] ; Pei-Yan He [République populaire de Chine] ; Jin-Lei Guo [République populaire de Chine] ; Yong-Liang Lei [République populaire de Chine] ; Zhong-Wen Chen [République populaire de Chine]Source :
- Oncotarget [ 1949-2553 ] ; 2017.
Abstract
We utilized one-step multiplex reverse transcription-PCR (RT-PCR) and Luminex xMAP technology to develop a respiratory multiplex liquid-chip assay (rMLA) for simultaneous detection of 6 common respiratory viruses, including influenza virus type A (FluA) and type B (FluB), para-influenza virus type 3 (PIV-3), respiratory syncytial virus (RSV), human metapneumovirus (MPV) and a threatening virus to China, Middle East Respiratory Syndrome coronavirus (MERS-CoV). Performance of rMLA was evaluated by comparing with real-time RT-PCR. Detection data from clinical specimens showed that the rMLA had diagnostic sensitivities of 97.10% for FluA, 94.59% for FluB, 98.68% for PIV-3, 94.87% for RSV and 95.92% for MPV (No Data for MERS-CoV due to the lack of positive specimens). Data of analytical sensitivities showed that the detection limits of the rMLA assay were 5-25 viral RNA copies per μl for FluA, FluB, PIV-3 and MERS-CoV, approximate to the real-time RT-PCR assay; while the values were 8 and 22copies/μl for MPV and RSV, lower than the real-time RT-PCR(78 and 114 copies/μl respectively). The results indicated that the rMLA is a sensitive, specific detection tool and comparable to real-time RT-PCR, especially suitable for high-throughput detection of respiratory specimens.
DOI: 10.18632/oncotarget.18533
PubMed: 29228581
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China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Municipal Center for Disease Control and Prevention, Jiaxing 314050</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:29228581</idno><idno type="pmid">29228581</idno><idno type="doi">10.18632/oncotarget.18533</idno><idno type="wicri:Area/PubMed/Corpus">000A56</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000A56</idno><idno type="wicri:Area/PubMed/Curation">000A56</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000A56</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A multiplex liquid-chip assay based on Luminex xMAP technology for simultaneous detection of six common respiratory viruses.</title><author><name sortKey="Yan, Yong" sort="Yan, Yong" uniqKey="Yan Y" first="Yong" last="Yan">Yong Yan</name><affiliation wicri:level="1"><nlm:affiliation>Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Municipal Center for Disease Control and Prevention, Jiaxing 314050, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Municipal Center for Disease Control and Prevention, Jiaxing 314050</wicri:regionArea></affiliation></author><author><name sortKey="Luo, Jian Yong" sort="Luo, Jian Yong" uniqKey="Luo J" first="Jian-Yong" last="Luo">Jian-Yong Luo</name><affiliation wicri:level="1"><nlm:affiliation>Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Municipal Center for Disease Control and Prevention, Jiaxing 314050, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Municipal Center for Disease Control and Prevention, Jiaxing 314050</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Yin" sort="Chen, Yin" uniqKey="Chen Y" first="Yin" last="Chen">Yin Chen</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Heng Hui" sort="Wang, Heng Hui" uniqKey="Wang H" first="Heng-Hui" last="Wang">Heng-Hui Wang</name><affiliation wicri:level="1"><nlm:affiliation>Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Municipal Center for Disease Control and Prevention, Jiaxing 314050, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Municipal Center for Disease Control and Prevention, Jiaxing 314050</wicri:regionArea></affiliation></author><author><name sortKey="Zhu, Guo Ying" sort="Zhu, Guo Ying" uniqKey="Zhu G" first="Guo-Ying" last="Zhu">Guo-Ying Zhu</name><affiliation wicri:level="1"><nlm:affiliation>Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Municipal Center for Disease Control and Prevention, Jiaxing 314050, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Municipal Center for Disease Control and Prevention, Jiaxing 314050</wicri:regionArea></affiliation></author><author><name sortKey="He, Pei Yan" sort="He, Pei Yan" uniqKey="He P" first="Pei-Yan" last="He">Pei-Yan He</name><affiliation wicri:level="1"><nlm:affiliation>Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Municipal Center for Disease Control and Prevention, Jiaxing 314050, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Municipal Center for Disease Control and Prevention, Jiaxing 314050</wicri:regionArea></affiliation></author><author><name sortKey="Guo, Jin Lei" sort="Guo, Jin Lei" uniqKey="Guo J" first="Jin-Lei" last="Guo">Jin-Lei Guo</name><affiliation wicri:level="1"><nlm:affiliation>Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Municipal Center for Disease Control and Prevention, Jiaxing 314050, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Municipal Center for Disease Control and Prevention, Jiaxing 314050</wicri:regionArea></affiliation></author><author><name sortKey="Lei, Yong Liang" sort="Lei, Yong Liang" uniqKey="Lei Y" first="Yong-Liang" last="Lei">Yong-Liang Lei</name><affiliation wicri:level="1"><nlm:affiliation>Lishui Municipal Center for Disease Control and Prevention, Lishui 323000, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Lishui Municipal Center for Disease Control and Prevention, Lishui 323000</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Zhong Wen" sort="Chen, Zhong Wen" uniqKey="Chen Z" first="Zhong-Wen" last="Chen">Zhong-Wen Chen</name><affiliation wicri:level="1"><nlm:affiliation>Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Municipal Center for Disease Control and Prevention, Jiaxing 314050, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Municipal Center for Disease Control and Prevention, Jiaxing 314050</wicri:regionArea></affiliation></author></analytic><series><title level="j">Oncotarget</title><idno type="eISSN">1949-2553</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We utilized one-step multiplex reverse transcription-PCR (RT-PCR) and Luminex xMAP technology to develop a respiratory multiplex liquid-chip assay (rMLA) for simultaneous detection of 6 common respiratory viruses, including influenza virus type A (FluA) and type B (FluB), para-influenza virus type 3 (PIV-3), respiratory syncytial virus (RSV), human metapneumovirus (MPV) and a threatening virus to China, Middle East Respiratory Syndrome coronavirus (MERS-CoV). Performance of rMLA was evaluated by comparing with real-time RT-PCR. Detection data from clinical specimens showed that the rMLA had diagnostic sensitivities of 97.10% for FluA, 94.59% for FluB, 98.68% for PIV-3, 94.87% for RSV and 95.92% for MPV (No Data for MERS-CoV due to the lack of positive specimens). Data of analytical sensitivities showed that the detection limits of the rMLA assay were 5-25 viral RNA copies per μl for FluA, FluB, PIV-3 and MERS-CoV, approximate to the real-time RT-PCR assay; while the values were 8 and 22copies/μl for MPV and RSV, lower than the real-time RT-PCR(78 and 114 copies/μl respectively). The results indicated that the rMLA is a sensitive, specific detection tool and comparable to real-time RT-PCR, especially suitable for high-throughput detection of respiratory specimens.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">29228581</PMID><DateRevised><Year>2019</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1949-2553</ISSN><JournalIssue CitedMedium="Internet"><Volume>8</Volume><Issue>57</Issue><PubDate><Year>2017</Year><Month>Nov</Month><Day>14</Day></PubDate></JournalIssue><Title>Oncotarget</Title><ISOAbbreviation>Oncotarget</ISOAbbreviation></Journal><ArticleTitle>A multiplex liquid-chip assay based on Luminex xMAP technology for simultaneous detection of six common respiratory viruses.</ArticleTitle><Pagination><MedlinePgn>96913-96923</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.18632/oncotarget.18533</ELocationID><Abstract><AbstractText>We utilized one-step multiplex reverse transcription-PCR (RT-PCR) and Luminex xMAP technology to develop a respiratory multiplex liquid-chip assay (rMLA) for simultaneous detection of 6 common respiratory viruses, including influenza virus type A (FluA) and type B (FluB), para-influenza virus type 3 (PIV-3), respiratory syncytial virus (RSV), human metapneumovirus (MPV) and a threatening virus to China, Middle East Respiratory Syndrome coronavirus (MERS-CoV). Performance of rMLA was evaluated by comparing with real-time RT-PCR. Detection data from clinical specimens showed that the rMLA had diagnostic sensitivities of 97.10% for FluA, 94.59% for FluB, 98.68% for PIV-3, 94.87% for RSV and 95.92% for MPV (No Data for MERS-CoV due to the lack of positive specimens). Data of analytical sensitivities showed that the detection limits of the rMLA assay were 5-25 viral RNA copies per μl for FluA, FluB, PIV-3 and MERS-CoV, approximate to the real-time RT-PCR assay; while the values were 8 and 22copies/μl for MPV and RSV, lower than the real-time RT-PCR(78 and 114 copies/μl respectively). The results indicated that the rMLA is a sensitive, specific detection tool and comparable to real-time RT-PCR, especially suitable for high-throughput detection of respiratory specimens.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yan</LastName><ForeName>Yong</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Municipal Center for Disease Control and Prevention, Jiaxing 314050, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Luo</LastName><ForeName>Jian-Yong</ForeName><Initials>JY</Initials><AffiliationInfo><Affiliation>Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Municipal Center for Disease Control and Prevention, Jiaxing 314050, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Yin</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Institute of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Heng-Hui</ForeName><Initials>HH</Initials><AffiliationInfo><Affiliation>Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Municipal Center for Disease Control and Prevention, Jiaxing 314050, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Guo-Ying</ForeName><Initials>GY</Initials><AffiliationInfo><Affiliation>Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Municipal Center for Disease Control and Prevention, Jiaxing 314050, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Pei-Yan</ForeName><Initials>PY</Initials><AffiliationInfo><Affiliation>Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Municipal Center for Disease Control and Prevention, Jiaxing 314050, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Jin-Lei</ForeName><Initials>JL</Initials><AffiliationInfo><Affiliation>Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Municipal Center for Disease Control and Prevention, Jiaxing 314050, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lei</LastName><ForeName>Yong-Liang</ForeName><Initials>YL</Initials><AffiliationInfo><Affiliation>Lishui Municipal Center for Disease Control and Prevention, Lishui 323000, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Zhong-Wen</ForeName><Initials>ZW</Initials><AffiliationInfo><Affiliation>Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Municipal Center for Disease Control and Prevention, Jiaxing 314050, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>06</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Oncotarget</MedlineTA><NlmUniqueID>101532965</NlmUniqueID><ISSNLinking>1949-2553</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Luminex</Keyword><Keyword MajorTopicYN="N">multiplex detection</Keyword><Keyword MajorTopicYN="N">real-time RT-PCR</Keyword><Keyword MajorTopicYN="N">respiratory virus</Keyword><Keyword MajorTopicYN="N">xMAP</Keyword></KeywordList><CoiStatement>CONFLICTS OF INTEREST All authors declare that they have no conflicts of interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>12</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>06</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>12</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>12</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>12</Month><Day>13</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29228581</ArticleId><ArticleId IdType="doi">10.18632/oncotarget.18533</ArticleId><ArticleId IdType="pii">18533</ArticleId><ArticleId IdType="pmc">PMC5722533</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Transpl Infect Dis. 2009 Aug;11(4):298-303</Citation><ArticleIdList><ArticleId IdType="pubmed">19453994</ArticleId></ArticleIdList></Reference><Reference><Citation>Euro Surveill. 2015 Jun 25;20(25):1-6</Citation><ArticleIdList><ArticleId 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