Receptor Usage of a Novel Bat Lineage C Betacoronavirus Reveals Evolution of Middle East Respiratory Syndrome-Related Coronavirus Spike Proteins for Human Dipeptidyl Peptidase 4 Binding.
Identifieur interne : 000A18 ( PubMed/Curation ); précédent : 000A17; suivant : 000A19Receptor Usage of a Novel Bat Lineage C Betacoronavirus Reveals Evolution of Middle East Respiratory Syndrome-Related Coronavirus Spike Proteins for Human Dipeptidyl Peptidase 4 Binding.
Auteurs : Susanna K P. Lau [République populaire de Chine] ; Libiao Zhang [République populaire de Chine] ; Hayes K H. Luk [République populaire de Chine] ; Lifeng Xiong [République populaire de Chine] ; Xingwen Peng [République populaire de Chine] ; Kenneth S M. Li [République populaire de Chine] ; Xiangyang He [République populaire de Chine] ; Pyrear Su-Hui Zhao [République populaire de Chine] ; Rachel Y Y. Fan [République populaire de Chine] ; Antonio C P. Wong [République populaire de Chine] ; Syed Shakeel Ahmed [République populaire de Chine] ; Jian-Piao Cai [République populaire de Chine] ; Jasper F W. Chan [République populaire de Chine] ; Yinyan Sun [République populaire de Chine] ; Dongyan Jin [République populaire de Chine] ; Honglin Chen [République populaire de Chine] ; Terrence C K. Lau [République populaire de Chine] ; Raven K H. Kok [République populaire de Chine] ; Wenhui Li [République populaire de Chine] ; Kwok-Yung Yuen [République populaire de Chine] ; Patrick C Y. Woo [République populaire de Chine]Source :
- The Journal of infectious diseases [ 1537-6613 ] ; 2018.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN, Animaux, Cellules HEK293, Chiroptera, Dipeptidyl peptidase 4 (métabolisme), Glycoprotéine de spicule des coronavirus (génétique), Glycoprotéine de spicule des coronavirus (métabolisme), Humains, Liaison aux protéines, Phylogénie, Pénétration virale, Récepteurs viraux (métabolisme), Évolution moléculaire.
- MESH :
- génétique : Glycoprotéine de spicule des coronavirus.
- métabolisme : Dipeptidyl peptidase 4, Glycoprotéine de spicule des coronavirus, Récepteurs viraux.
- Analyse de séquence d'ADN, Animaux, Cellules HEK293, Chiroptera, Humains, Liaison aux protéines, Phylogénie, Pénétration virale, Évolution moléculaire.
English descriptors
- KwdEn :
- Animals, Betacoronavirus (classification), Betacoronavirus (genetics), Betacoronavirus (isolation & purification), Betacoronavirus (physiology), Chiroptera, Dipeptidyl Peptidase 4 (metabolism), Evolution, Molecular, HEK293 Cells, Humans, Phylogeny, Protein Binding, Receptors, Virus (metabolism), Sequence Analysis, DNA, Spike Glycoprotein, Coronavirus (genetics), Spike Glycoprotein, Coronavirus (metabolism), Virus Internalization.
- MESH :
- chemical , genetics : Spike Glycoprotein, Coronavirus.
- chemical , metabolism : Dipeptidyl Peptidase 4, Receptors, Virus, Spike Glycoprotein, Coronavirus.
- classification : Betacoronavirus.
- genetics : Betacoronavirus.
- isolation & purification : Betacoronavirus.
- physiology : Betacoronavirus.
- Animals, Chiroptera, Evolution, Molecular, HEK293 Cells, Humans, Phylogeny, Protein Binding, Sequence Analysis, DNA, Virus Internalization.
Abstract
Although bats are known to harbor Middle East Respiratory Syndrome coronavirus (MERS-CoV)-related viruses, the role of bats in the evolutionary origin and pathway remains obscure. We identified a novel MERS-CoV-related betacoronavirus, Hp-BatCoV HKU25, from Chinese pipistrelle bats. Although it is closely related to MERS-CoV in most genome regions, its spike protein occupies a phylogenetic position between that of Ty-BatCoV HKU4 and Pi-BatCoV HKU5. Because Ty-BatCoV HKU4 but not Pi-BatCoV HKU5 can use the MERS-CoV receptor human dipeptidyl peptidase 4 (hDPP4) for cell entry, we tested the ability of Hp-BatCoV HKU25 to bind and use hDPP4. The HKU25-receptor binding domain (RBD) can bind to hDPP4 protein and hDPP4-expressing cells, but it does so with lower efficiency than that of MERS-RBD. Pseudovirus assays showed that HKU25-spike can use hDPP4 for entry to hDPP4-expressing cells, although with lower efficiency than that of MERS-spike and HKU4-spike. Our findings support a bat origin of MERS-CoV and suggest that bat CoV spike proteins may have evolved in a stepwise manner for binding to hDPP4.
DOI: 10.1093/infdis/jiy018
PubMed: 29346682
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<author><name sortKey="Zhao, Pyrear Su Hui" sort="Zhao, Pyrear Su Hui" uniqKey="Zhao P" first="Pyrear Su-Hui" last="Zhao">Pyrear Su-Hui Zhao</name>
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<author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Receptor Usage of a Novel Bat Lineage C Betacoronavirus Reveals Evolution of Middle East Respiratory Syndrome-Related Coronavirus Spike Proteins for Human Dipeptidyl Peptidase 4 Binding.</title>
<author><name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K P" last="Lau">Susanna K P. Lau</name>
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<author><name sortKey="Zhang, Libiao" sort="Zhang, Libiao" uniqKey="Zhang L" first="Libiao" last="Zhang">Libiao Zhang</name>
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<country xml:lang="fr">République populaire de Chine</country>
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<author><name sortKey="Luk, Hayes K H" sort="Luk, Hayes K H" uniqKey="Luk H" first="Hayes K H" last="Luk">Hayes K H. Luk</name>
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<country xml:lang="fr">République populaire de Chine</country>
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<author><name sortKey="Xiong, Lifeng" sort="Xiong, Lifeng" uniqKey="Xiong L" first="Lifeng" last="Xiong">Lifeng Xiong</name>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology The University of Hong Kong</wicri:regionArea>
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<author><name sortKey="Peng, Xingwen" sort="Peng, Xingwen" uniqKey="Peng X" first="Xingwen" last="Peng">Xingwen Peng</name>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, and Guangdong Institute of Applied Biological Resources, Guangzhou, Guangdong Province</wicri:regionArea>
</affiliation>
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<author><name sortKey="Li, Kenneth S M" sort="Li, Kenneth S M" uniqKey="Li K" first="Kenneth S M" last="Li">Kenneth S M. Li</name>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology The University of Hong Kong</wicri:regionArea>
</affiliation>
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<author><name sortKey="He, Xiangyang" sort="He, Xiangyang" uniqKey="He X" first="Xiangyang" last="He">Xiangyang He</name>
<affiliation wicri:level="1"><nlm:affiliation>Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, and Guangdong Institute of Applied Biological Resources, Guangzhou, Guangdong Province, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, and Guangdong Institute of Applied Biological Resources, Guangzhou, Guangdong Province</wicri:regionArea>
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<author><name sortKey="Zhao, Pyrear Su Hui" sort="Zhao, Pyrear Su Hui" uniqKey="Zhao P" first="Pyrear Su-Hui" last="Zhao">Pyrear Su-Hui Zhao</name>
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<author><name sortKey="Fan, Rachel Y Y" sort="Fan, Rachel Y Y" uniqKey="Fan R" first="Rachel Y Y" last="Fan">Rachel Y Y. Fan</name>
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<author><name sortKey="Wong, Antonio C P" sort="Wong, Antonio C P" uniqKey="Wong A" first="Antonio C P" last="Wong">Antonio C P. Wong</name>
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<author><name sortKey="Ahmed, Syed Shakeel" sort="Ahmed, Syed Shakeel" uniqKey="Ahmed S" first="Syed Shakeel" last="Ahmed">Syed Shakeel Ahmed</name>
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<wicri:regionArea>Department of Microbiology The University of Hong Kong</wicri:regionArea>
</affiliation>
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<author><name sortKey="Cai, Jian Piao" sort="Cai, Jian Piao" uniqKey="Cai J" first="Jian-Piao" last="Cai">Jian-Piao Cai</name>
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<wicri:regionArea>Department of Microbiology The University of Hong Kong</wicri:regionArea>
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<author><name sortKey="Chan, Jasper F W" sort="Chan, Jasper F W" uniqKey="Chan J" first="Jasper F W" last="Chan">Jasper F W. Chan</name>
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<author><name sortKey="Sun, Yinyan" sort="Sun, Yinyan" uniqKey="Sun Y" first="Yinyan" last="Sun">Yinyan Sun</name>
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<author><name sortKey="Jin, Dongyan" sort="Jin, Dongyan" uniqKey="Jin D" first="Dongyan" last="Jin">Dongyan Jin</name>
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<author><name sortKey="Chen, Honglin" sort="Chen, Honglin" uniqKey="Chen H" first="Honglin" last="Chen">Honglin Chen</name>
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<author><name sortKey="Lau, Terrence C K" sort="Lau, Terrence C K" uniqKey="Lau T" first="Terrence C K" last="Lau">Terrence C K. Lau</name>
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<author><name sortKey="Kok, Raven K H" sort="Kok, Raven K H" uniqKey="Kok R" first="Raven K H" last="Kok">Raven K H. Kok</name>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases The University of Hong Kong</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Li, Wenhui" sort="Li, Wenhui" uniqKey="Li W" first="Wenhui" last="Li">Wenhui Li</name>
<affiliation wicri:level="1"><nlm:affiliation>National Institute of Biological Sciences, Zhongguancun Life Science Park, Changping, Beijing, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
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<term>Phylogeny</term>
<term>Protein Binding</term>
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<front><div type="abstract" xml:lang="en">Although bats are known to harbor Middle East Respiratory Syndrome coronavirus (MERS-CoV)-related viruses, the role of bats in the evolutionary origin and pathway remains obscure. We identified a novel MERS-CoV-related betacoronavirus, Hp-BatCoV HKU25, from Chinese pipistrelle bats. Although it is closely related to MERS-CoV in most genome regions, its spike protein occupies a phylogenetic position between that of Ty-BatCoV HKU4 and Pi-BatCoV HKU5. Because Ty-BatCoV HKU4 but not Pi-BatCoV HKU5 can use the MERS-CoV receptor human dipeptidyl peptidase 4 (hDPP4) for cell entry, we tested the ability of Hp-BatCoV HKU25 to bind and use hDPP4. The HKU25-receptor binding domain (RBD) can bind to hDPP4 protein and hDPP4-expressing cells, but it does so with lower efficiency than that of MERS-RBD. Pseudovirus assays showed that HKU25-spike can use hDPP4 for entry to hDPP4-expressing cells, although with lower efficiency than that of MERS-spike and HKU4-spike. Our findings support a bat origin of MERS-CoV and suggest that bat CoV spike proteins may have evolved in a stepwise manner for binding to hDPP4.</div>
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<DateCompleted><Year>2019</Year>
<Month>09</Month>
<Day>12</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>04</Month>
<Day>03</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1537-6613</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>218</Volume>
<Issue>2</Issue>
<PubDate><Year>2018</Year>
<Month>06</Month>
<Day>20</Day>
</PubDate>
</JournalIssue>
<Title>The Journal of infectious diseases</Title>
<ISOAbbreviation>J. Infect. Dis.</ISOAbbreviation>
</Journal>
<ArticleTitle>Receptor Usage of a Novel Bat Lineage C Betacoronavirus Reveals Evolution of Middle East Respiratory Syndrome-Related Coronavirus Spike Proteins for Human Dipeptidyl Peptidase 4 Binding.</ArticleTitle>
<Pagination><MedlinePgn>197-207</MedlinePgn>
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<ELocationID EIdType="doi" ValidYN="Y">10.1093/infdis/jiy018</ELocationID>
<Abstract><AbstractText>Although bats are known to harbor Middle East Respiratory Syndrome coronavirus (MERS-CoV)-related viruses, the role of bats in the evolutionary origin and pathway remains obscure. We identified a novel MERS-CoV-related betacoronavirus, Hp-BatCoV HKU25, from Chinese pipistrelle bats. Although it is closely related to MERS-CoV in most genome regions, its spike protein occupies a phylogenetic position between that of Ty-BatCoV HKU4 and Pi-BatCoV HKU5. Because Ty-BatCoV HKU4 but not Pi-BatCoV HKU5 can use the MERS-CoV receptor human dipeptidyl peptidase 4 (hDPP4) for cell entry, we tested the ability of Hp-BatCoV HKU25 to bind and use hDPP4. The HKU25-receptor binding domain (RBD) can bind to hDPP4 protein and hDPP4-expressing cells, but it does so with lower efficiency than that of MERS-RBD. Pseudovirus assays showed that HKU25-spike can use hDPP4 for entry to hDPP4-expressing cells, although with lower efficiency than that of MERS-spike and HKU4-spike. Our findings support a bat origin of MERS-CoV and suggest that bat CoV spike proteins may have evolved in a stepwise manner for binding to hDPP4.</AbstractText>
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<ForeName>Susanna K P</ForeName>
<Initials>SKP</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Emerging Infectious Diseases The University of Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Microbiology The University of Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Carol Yu Centre for Infection The University of Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, China.</Affiliation>
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<ForeName>Lifeng</ForeName>
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<ForeName>Xingwen</ForeName>
<Initials>X</Initials>
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<ForeName>Antonio C P</ForeName>
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<Author ValidYN="Y"><LastName>Ahmed</LastName>
<ForeName>Syed Shakeel</ForeName>
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<ForeName>Jian-Piao</ForeName>
<Initials>JP</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology The University of Hong Kong, China.</Affiliation>
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<ForeName>Jasper F W</ForeName>
<Initials>JFW</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Emerging Infectious Diseases The University of Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Microbiology The University of Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Carol Yu Centre for Infection The University of Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, China.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Sun</LastName>
<ForeName>Yinyan</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>National Institute of Biological Sciences, Zhongguancun Life Science Park, Changping, Beijing, China.</Affiliation>
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</Author>
<Author ValidYN="Y"><LastName>Jin</LastName>
<ForeName>Dongyan</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>School of Biomedical Sciences, The University of Hong Kong, China.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Honglin</ForeName>
<Initials>H</Initials>
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</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Microbiology The University of Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Carol Yu Centre for Infection The University of Hong Kong, China.</Affiliation>
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<AffiliationInfo><Affiliation>Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, China.</Affiliation>
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<Author ValidYN="Y"><LastName>Lau</LastName>
<ForeName>Terrence C K</ForeName>
<Initials>TCK</Initials>
<AffiliationInfo><Affiliation>Department of Biomedical Sciences, City University of Hong Kong, China.</Affiliation>
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<Author ValidYN="Y"><LastName>Kok</LastName>
<ForeName>Raven K H</ForeName>
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</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Microbiology The University of Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Carol Yu Centre for Infection The University of Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, China.</Affiliation>
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<Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>Wenhui</ForeName>
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<Author ValidYN="Y"><LastName>Yuen</LastName>
<ForeName>Kwok-Yung</ForeName>
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<AffiliationInfo><Affiliation>Department of Microbiology The University of Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Carol Yu Centre for Infection The University of Hong Kong, China.</Affiliation>
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</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Microbiology The University of Hong Kong, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Carol Yu Centre for Infection The University of Hong Kong, China.</Affiliation>
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<AffiliationInfo><Affiliation>Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, China.</Affiliation>
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