Self-Assembling Supramolecular Nanostructures Constructed from de Novo Extender Protein Nanobuilding Blocks.
Identifieur interne : 000919 ( PubMed/Curation ); précédent : 000918; suivant : 000920Self-Assembling Supramolecular Nanostructures Constructed from de Novo Extender Protein Nanobuilding Blocks.
Auteurs : Naoya Kobayashi [Japon] ; Kouichi Inano ; Kenji Sasahara ; Takaaki Sato [Japon] ; Keisuke Miyazawa [Japon] ; Takeshi Fukuma [Japon] ; Michael H. Hecht [États-Unis] ; Chihong Song [Japon] ; Kazuyoshi Murata [Japon] ; Ryoichi Arai [Japon]Source :
- ACS synthetic biology [ 2161-5063 ] ; 2018.
Descripteurs français
- KwdFr :
- Chromatographie sur gel, Diffraction des rayons X, Diffusion aux petits angles, Dénaturation des protéines, Escherichia coli (génétique), Ingénierie des protéines (), Microscopie à force atomique, Microscopie électronique à transmission, Nanostructures (), Protéines recombinantes (), Protéines recombinantes (génétique), Protéines recombinantes (métabolisme), Repliement des protéines.
- MESH :
- génétique : Escherichia coli, Protéines recombinantes.
- métabolisme : Protéines recombinantes.
- Chromatographie sur gel, Diffraction des rayons X, Diffusion aux petits angles, Dénaturation des protéines, Ingénierie des protéines, Microscopie à force atomique, Microscopie électronique à transmission, Nanostructures, Protéines recombinantes, Repliement des protéines.
English descriptors
- KwdEn :
- Chromatography, Gel, Escherichia coli (genetics), Microscopy, Atomic Force, Microscopy, Electron, Transmission, Nanostructures (chemistry), Protein Denaturation, Protein Engineering (methods), Protein Refolding, Recombinant Proteins (chemistry), Recombinant Proteins (genetics), Recombinant Proteins (metabolism), Scattering, Small Angle, X-Ray Diffraction.
- MESH :
- chemical , chemistry : Recombinant Proteins.
- chemistry : Nanostructures.
- genetics : Escherichia coli, Recombinant Proteins.
- chemical , metabolism : Recombinant Proteins.
- methods : Protein Engineering.
- Chromatography, Gel, Microscopy, Atomic Force, Microscopy, Electron, Transmission, Protein Denaturation, Protein Refolding, Scattering, Small Angle, X-Ray Diffraction.
Abstract
The design of novel proteins that self-assemble into supramolecular complexes is important for development in nanobiotechnology and synthetic biology. Recently, we designed and created a protein nanobuilding block (PN-Block), WA20-foldon, by fusing an intermolecularly folded dimeric de novo WA20 protein and a trimeric foldon domain of T4 phage fibritin (Kobayashi et al., J. Am. Chem. Soc. 2015, 137, 11285). WA20-foldon formed several types of self-assembling nanoarchitectures in multiples of 6-mers, including a barrel-like hexamer and a tetrahedron-like dodecamer. In this study, to construct chain-like polymeric nanostructures, we designed de novo extender protein nanobuilding blocks (ePN-Blocks) by tandemly fusing two de novo binary-patterned WA20 proteins with various linkers. The ePN-Blocks with long helical linkers or flexible linkers were expressed in soluble fractions of Escherichia coli, and the purified ePN-Blocks were analyzed by native PAGE, size exclusion chromatography-multiangle light scattering (SEC-MALS), small-angle X-ray scattering (SAXS), and transmission electron microscopy. These results suggest formation of various structural homo-oligomers. Subsequently, we reconstructed hetero-oligomeric complexes from extender and stopper PN-Blocks by denaturation and refolding. The present SEC-MALS and SAXS analyses show that extender and stopper PN-Block (esPN-Block) heterocomplexes formed different types of extended chain-like conformations depending on their linker types. Moreover, atomic force microscopy imaging in liquid suggests that the esPN-Block heterocomplexes with metal ions further self-assembled into supramolecular nanostructures on mica surfaces. Taken together, the present data demonstrate that the design and construction of self-assembling PN-Blocks using de novo proteins is a useful strategy for building polymeric nanoarchitectures of supramolecular protein complexes.
DOI: 10.1021/acssynbio.8b00007
PubMed: 29690759
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<front><div type="abstract" xml:lang="en">The design of novel proteins that self-assemble into supramolecular complexes is important for development in nanobiotechnology and synthetic biology. Recently, we designed and created a protein nanobuilding block (PN-Block), WA20-foldon, by fusing an intermolecularly folded dimeric de novo WA20 protein and a trimeric foldon domain of T4 phage fibritin (Kobayashi et al., J. Am. Chem. Soc. 2015, 137, 11285). WA20-foldon formed several types of self-assembling nanoarchitectures in multiples of 6-mers, including a barrel-like hexamer and a tetrahedron-like dodecamer. In this study, to construct chain-like polymeric nanostructures, we designed de novo extender protein nanobuilding blocks (ePN-Blocks) by tandemly fusing two de novo binary-patterned WA20 proteins with various linkers. The ePN-Blocks with long helical linkers or flexible linkers were expressed in soluble fractions of Escherichia coli, and the purified ePN-Blocks were analyzed by native PAGE, size exclusion chromatography-multiangle light scattering (SEC-MALS), small-angle X-ray scattering (SAXS), and transmission electron microscopy. These results suggest formation of various structural homo-oligomers. Subsequently, we reconstructed hetero-oligomeric complexes from extender and stopper PN-Blocks by denaturation and refolding. The present SEC-MALS and SAXS analyses show that extender and stopper PN-Block (esPN-Block) heterocomplexes formed different types of extended chain-like conformations depending on their linker types. Moreover, atomic force microscopy imaging in liquid suggests that the esPN-Block heterocomplexes with metal ions further self-assembled into supramolecular nanostructures on mica surfaces. Taken together, the present data demonstrate that the design and construction of self-assembling PN-Blocks using de novo proteins is a useful strategy for building polymeric nanoarchitectures of supramolecular protein complexes.</div>
</front>
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<Abstract><AbstractText>The design of novel proteins that self-assemble into supramolecular complexes is important for development in nanobiotechnology and synthetic biology. Recently, we designed and created a protein nanobuilding block (PN-Block), WA20-foldon, by fusing an intermolecularly folded dimeric de novo WA20 protein and a trimeric foldon domain of T4 phage fibritin (Kobayashi et al., J. Am. Chem. Soc. 2015, 137, 11285). WA20-foldon formed several types of self-assembling nanoarchitectures in multiples of 6-mers, including a barrel-like hexamer and a tetrahedron-like dodecamer. In this study, to construct chain-like polymeric nanostructures, we designed de novo extender protein nanobuilding blocks (ePN-Blocks) by tandemly fusing two de novo binary-patterned WA20 proteins with various linkers. The ePN-Blocks with long helical linkers or flexible linkers were expressed in soluble fractions of Escherichia coli, and the purified ePN-Blocks were analyzed by native PAGE, size exclusion chromatography-multiangle light scattering (SEC-MALS), small-angle X-ray scattering (SAXS), and transmission electron microscopy. These results suggest formation of various structural homo-oligomers. Subsequently, we reconstructed hetero-oligomeric complexes from extender and stopper PN-Blocks by denaturation and refolding. The present SEC-MALS and SAXS analyses show that extender and stopper PN-Block (esPN-Block) heterocomplexes formed different types of extended chain-like conformations depending on their linker types. Moreover, atomic force microscopy imaging in liquid suggests that the esPN-Block heterocomplexes with metal ions further self-assembled into supramolecular nanostructures on mica surfaces. Taken together, the present data demonstrate that the design and construction of self-assembling PN-Blocks using de novo proteins is a useful strategy for building polymeric nanoarchitectures of supramolecular protein complexes.</AbstractText>
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<ForeName>Naoya</ForeName>
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<AffiliationInfo><Affiliation>Institute for Biomedical Sciences , Interdisciplinary Cluster for Cutting Edge Research, Shinshu University , Matsumoto , Nagano 390-8621 , Japan.</Affiliation>
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<AffiliationInfo><Affiliation>Department of Supramolecular Complexes , Research Center for Fungal and Microbial Dynamism, Shinshu University , Minamiminowa , Nagano 399-4598 , Japan.</Affiliation>
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<MeshHeading><DescriptorName UI="D018625" MajorTopicYN="N">Microscopy, Atomic Force</DescriptorName>
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<MeshHeading><DescriptorName UI="D046529" MajorTopicYN="N">Microscopy, Electron, Transmission</DescriptorName>
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<MeshHeading><DescriptorName UI="D011489" MajorTopicYN="N">Protein Denaturation</DescriptorName>
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<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">de novo protein</Keyword>
<Keyword MajorTopicYN="Y">nanostructure</Keyword>
<Keyword MajorTopicYN="Y">protein engineering</Keyword>
<Keyword MajorTopicYN="Y">protein nanobuilding block</Keyword>
<Keyword MajorTopicYN="Y">protein-based supramolecular polymers</Keyword>
<Keyword MajorTopicYN="Y">self-assembly</Keyword>
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<ArticleIdList><ArticleId IdType="pubmed">29690759</ArticleId>
<ArticleId IdType="doi">10.1021/acssynbio.8b00007</ArticleId>
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