The Human Sodium Iodide Symporter as a Reporter Gene for Studying Middle East Respiratory Syndrome Coronavirus Pathogenesis.
Identifieur interne : 000709 ( PubMed/Curation ); précédent : 000708; suivant : 000710The Human Sodium Iodide Symporter as a Reporter Gene for Studying Middle East Respiratory Syndrome Coronavirus Pathogenesis.
Auteurs : Svetlana Chefer [États-Unis] ; Jurgen Seidel [États-Unis] ; Adam S. Cockrell [États-Unis] ; Boyd Yount [États-Unis] ; Jeffrey Solomon [États-Unis] ; Katie R. Hagen [États-Unis] ; David X. Liu [États-Unis] ; Louis M. Huzella [États-Unis] ; Mia R. Kumar [États-Unis] ; Elena Postnikova [États-Unis] ; J Kyle Bohannon [États-Unis] ; Matthew G. Lackemeyer [États-Unis] ; Kurt Cooper [États-Unis] ; Ariel Endlich-Frazier [États-Unis] ; Heema Sharma [États-Unis] ; David Thomasson [États-Unis] ; Christopher Bartos [États-Unis] ; Philip J. Sayre [États-Unis] ; Amy Sims [États-Unis] ; Julie Dyall [États-Unis] ; Michael R. Holbrook [États-Unis] ; Peter B. Jahrling [États-Unis] ; Ralph S. Baric [États-Unis] ; Reed F. Johnson [Indonésie]Source :
- mSphere [ 2379-5042 ] ; 2018.
Descripteurs français
- KwdFr :
- Animaux, Cellules Vero, Coronavirus du syndrome respiratoire du Moyen-Orient (croissance et développement), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Gènes rapporteurs, Infections à coronavirus (anatomopathologie), Instabilité du génome, Modèles animaux de maladie humaine, Mutagenèse par insertion, Souris transgéniques, Symporteurs (génétique), Symporteurs (métabolisme), Tomographie par émission monophotonique couplée à la tomodensitométrie ().
- MESH :
- anatomopathologie : Infections à coronavirus.
- croissance et développement : Coronavirus du syndrome respiratoire du Moyen-Orient.
- génétique : Coronavirus du syndrome respiratoire du Moyen-Orient, Symporteurs.
- métabolisme : Symporteurs.
- Animaux, Cellules Vero, Gènes rapporteurs, Instabilité du génome, Modèles animaux de maladie humaine, Mutagenèse par insertion, Souris transgéniques, Tomographie par émission monophotonique couplée à la tomodensitométrie.
English descriptors
- KwdEn :
- Animals, Chlorocebus aethiops, Coronavirus Infections (pathology), Disease Models, Animal, Genes, Reporter, Genomic Instability, Mice, Transgenic, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (growth & development), Mutagenesis, Insertional, Single Photon Emission Computed Tomography Computed Tomography (methods), Symporters (genetics), Symporters (metabolism), Vero Cells.
- MESH :
- chemical , genetics : Symporters.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- growth & development : Middle East Respiratory Syndrome Coronavirus.
- chemical , metabolism : Symporters.
- methods : Single Photon Emission Computed Tomography Computed Tomography.
- pathology : Coronavirus Infections.
- Animals, Chlorocebus aethiops, Disease Models, Animal, Genes, Reporter, Genomic Instability, Mice, Transgenic, Mutagenesis, Insertional, Vero Cells.
Abstract
Single photon emission computed tomography (SPECT) is frequently used in oncology and cardiology to evaluate disease progression and/or treatment efficacy. Such technology allows for real-time evaluation of disease progression and when applied to studying infectious diseases may provide insight into pathogenesis. Insertion of a SPECT-compatible reporter gene into a virus may provide insight into mechanisms of pathogenesis and viral tropism. The human sodium iodide symporter (hNIS), a SPECT and positron emission tomography reporter gene, was inserted into Middle East respiratory syndrome coronavirus (MERS-CoV), a recently emerged virus that can cause severe respiratory disease and death in afflicted humans to obtain a quantifiable and sensitive marker for viral replication to further MERS-CoV animal model development. The recombinant virus was evaluated for fitness, stability, and reporter gene functionality. The recombinant and parental viruses demonstrated equal fitness in terms of peak titer and replication kinetics, were stable for up to six in vitro passages, and were functional. Further in vivo evaluation indicated variable stability, but resolution limits hampered in vivo functional evaluation. These data support the further development of hNIS for monitoring infection in animal models of viral disease.IMPORTANCE Advanced medical imaging such as single photon emission computed tomography with computed tomography (SPECT/CT) enhances fields such as oncology and cardiology. Application of SPECT/CT, magnetic resonance imaging, and positron emission tomography to infectious disease may enhance pathogenesis studies and provide alternate biomarkers of disease progression. The experiments described in this article focus on insertion of a SPECT/CT-compatible reporter gene into MERS-CoV to demonstrate that a functional SPECT/CT reporter gene can be inserted into a virus.
DOI: 10.1128/mSphere.00540-18
PubMed: 30541777
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Holbrook</name><affiliation wicri:level="1"><nlm:affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea></affiliation></author><author><name sortKey="Jahrling, Peter B" sort="Jahrling, Peter B" uniqKey="Jahrling P" first="Peter B" last="Jahrling">Peter B. Jahrling</name><affiliation wicri:level="1"><nlm:affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea></affiliation></author><author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S" last="Baric">Ralph S. Baric</name><affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea></affiliation></author><author><name sortKey="Johnson, Reed F" sort="Johnson, Reed F" uniqKey="Johnson R" first="Reed F" last="Johnson">Reed F. Johnson</name><affiliation wicri:level="1"><nlm:affiliation>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA johnsonreed@niaid.nih.gov.</nlm:affiliation><country wicri:rule="url">Indonésie</country><wicri:regionArea>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:30541777</idno><idno type="pmid">30541777</idno><idno type="doi">10.1128/mSphere.00540-18</idno><idno type="wicri:Area/PubMed/Corpus">000709</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000709</idno><idno type="wicri:Area/PubMed/Curation">000709</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000709</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The Human Sodium Iodide Symporter as a Reporter Gene for Studying Middle East Respiratory Syndrome Coronavirus Pathogenesis.</title><author><name sortKey="Chefer, Svetlana" sort="Chefer, Svetlana" uniqKey="Chefer S" first="Svetlana" last="Chefer">Svetlana Chefer</name><affiliation wicri:level="1"><nlm:affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea></affiliation></author><author><name sortKey="Seidel, Jurgen" sort="Seidel, Jurgen" uniqKey="Seidel J" first="Jurgen" last="Seidel">Jurgen Seidel</name><affiliation wicri:level="1"><nlm:affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea></affiliation></author><author><name sortKey="Cockrell, Adam S" sort="Cockrell, Adam S" uniqKey="Cockrell A" first="Adam S" last="Cockrell">Adam S. Cockrell</name><affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea></affiliation></author><author><name sortKey="Yount, Boyd" sort="Yount, Boyd" uniqKey="Yount B" first="Boyd" last="Yount">Boyd Yount</name><affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea></affiliation></author><author><name sortKey="Solomon, Jeffrey" sort="Solomon, Jeffrey" uniqKey="Solomon J" first="Jeffrey" last="Solomon">Jeffrey Solomon</name><affiliation wicri:level="1"><nlm:affiliation>Clinical Research Directorate/Clinical Monitoring Research Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Clinical Research Directorate/Clinical Monitoring Research Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland</wicri:regionArea></affiliation></author><author><name sortKey="Hagen, Katie R" sort="Hagen, Katie R" uniqKey="Hagen K" first="Katie R" last="Hagen">Katie R. Hagen</name><affiliation wicri:level="1"><nlm:affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea></affiliation></author><author><name sortKey="Liu, David X" sort="Liu, David X" uniqKey="Liu D" first="David X" last="Liu">David X. Liu</name><affiliation wicri:level="1"><nlm:affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea></affiliation></author><author><name sortKey="Huzella, Louis M" sort="Huzella, Louis M" uniqKey="Huzella L" first="Louis M" last="Huzella">Louis M. Huzella</name><affiliation wicri:level="1"><nlm:affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea></affiliation></author><author><name sortKey="Kumar, Mia R" sort="Kumar, Mia R" uniqKey="Kumar M" first="Mia R" last="Kumar">Mia R. Kumar</name><affiliation wicri:level="1"><nlm:affiliation>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea></affiliation></author><author><name sortKey="Postnikova, Elena" sort="Postnikova, Elena" uniqKey="Postnikova E" first="Elena" last="Postnikova">Elena Postnikova</name><affiliation wicri:level="1"><nlm:affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea></affiliation></author><author><name sortKey="Bohannon, J Kyle" sort="Bohannon, J Kyle" uniqKey="Bohannon J" first="J Kyle" last="Bohannon">J Kyle Bohannon</name><affiliation wicri:level="1"><nlm:affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea></affiliation></author><author><name sortKey="Lackemeyer, Matthew G" sort="Lackemeyer, Matthew G" uniqKey="Lackemeyer M" first="Matthew G" last="Lackemeyer">Matthew G. Lackemeyer</name><affiliation wicri:level="1"><nlm:affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea></affiliation></author><author><name sortKey="Cooper, Kurt" sort="Cooper, Kurt" uniqKey="Cooper K" first="Kurt" last="Cooper">Kurt Cooper</name><affiliation wicri:level="1"><nlm:affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea></affiliation></author><author><name sortKey="Endlich Frazier, Ariel" sort="Endlich Frazier, Ariel" uniqKey="Endlich Frazier A" first="Ariel" last="Endlich-Frazier">Ariel Endlich-Frazier</name><affiliation wicri:level="1"><nlm:affiliation>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea></affiliation></author><author><name sortKey="Sharma, Heema" sort="Sharma, Heema" uniqKey="Sharma H" first="Heema" last="Sharma">Heema Sharma</name><affiliation wicri:level="1"><nlm:affiliation>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea></affiliation></author><author><name sortKey="Thomasson, David" sort="Thomasson, David" uniqKey="Thomasson D" first="David" last="Thomasson">David Thomasson</name><affiliation wicri:level="1"><nlm:affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea></affiliation></author><author><name sortKey="Bartos, Christopher" sort="Bartos, Christopher" uniqKey="Bartos C" first="Christopher" last="Bartos">Christopher Bartos</name><affiliation wicri:level="1"><nlm:affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea></affiliation></author><author><name sortKey="Sayre, Philip J" sort="Sayre, Philip J" uniqKey="Sayre P" first="Philip J" last="Sayre">Philip J. Sayre</name><affiliation wicri:level="1"><nlm:affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea></affiliation></author><author><name sortKey="Sims, Amy" sort="Sims, Amy" uniqKey="Sims A" first="Amy" last="Sims">Amy Sims</name><affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea></affiliation></author><author><name sortKey="Dyall, Julie" sort="Dyall, Julie" uniqKey="Dyall J" first="Julie" last="Dyall">Julie Dyall</name><affiliation wicri:level="1"><nlm:affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea></affiliation></author><author><name sortKey="Holbrook, Michael R" sort="Holbrook, Michael R" uniqKey="Holbrook M" first="Michael R" last="Holbrook">Michael R. Holbrook</name><affiliation wicri:level="1"><nlm:affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea></affiliation></author><author><name sortKey="Jahrling, Peter B" sort="Jahrling, Peter B" uniqKey="Jahrling P" first="Peter B" last="Jahrling">Peter B. Jahrling</name><affiliation wicri:level="1"><nlm:affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea></affiliation></author><author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S" last="Baric">Ralph S. Baric</name><affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea></affiliation></author><author><name sortKey="Johnson, Reed F" sort="Johnson, Reed F" uniqKey="Johnson R" first="Reed F" last="Johnson">Reed F. Johnson</name><affiliation wicri:level="1"><nlm:affiliation>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA johnsonreed@niaid.nih.gov.</nlm:affiliation><country wicri:rule="url">Indonésie</country><wicri:regionArea>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland</wicri:regionArea></affiliation></author></analytic><series><title level="j">mSphere</title><idno type="eISSN">2379-5042</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Coronavirus Infections (pathology)</term><term>Disease Models, Animal</term><term>Genes, Reporter</term><term>Genomic Instability</term><term>Mice, Transgenic</term><term>Middle East Respiratory Syndrome Coronavirus (genetics)</term><term>Middle East Respiratory Syndrome Coronavirus (growth & development)</term><term>Mutagenesis, Insertional</term><term>Single Photon Emission Computed Tomography Computed Tomography (methods)</term><term>Symporters (genetics)</term><term>Symporters (metabolism)</term><term>Vero Cells</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (croissance et développement)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term><term>Gènes rapporteurs</term><term>Infections à coronavirus (anatomopathologie)</term><term>Instabilité du génome</term><term>Modèles animaux de maladie humaine</term><term>Mutagenèse par insertion</term><term>Souris transgéniques</term><term>Symporteurs (génétique)</term><term>Symporteurs (métabolisme)</term><term>Tomographie par émission monophotonique couplée à la tomodensitométrie ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Symporters</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Symporteurs</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Symporters</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Single Photon Emission Computed Tomography Computed Tomography</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Symporteurs</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Disease Models, Animal</term><term>Genes, Reporter</term><term>Genomic Instability</term><term>Mice, Transgenic</term><term>Mutagenesis, Insertional</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Gènes rapporteurs</term><term>Instabilité du génome</term><term>Modèles animaux de maladie humaine</term><term>Mutagenèse par insertion</term><term>Souris transgéniques</term><term>Tomographie par émission monophotonique couplée à la tomodensitométrie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Single photon emission computed tomography (SPECT) is frequently used in oncology and cardiology to evaluate disease progression and/or treatment efficacy. Such technology allows for real-time evaluation of disease progression and when applied to studying infectious diseases may provide insight into pathogenesis. Insertion of a SPECT-compatible reporter gene into a virus may provide insight into mechanisms of pathogenesis and viral tropism. The human sodium iodide symporter (hNIS), a SPECT and positron emission tomography reporter gene, was inserted into Middle East respiratory syndrome coronavirus (MERS-CoV), a recently emerged virus that can cause severe respiratory disease and death in afflicted humans to obtain a quantifiable and sensitive marker for viral replication to further MERS-CoV animal model development. The recombinant virus was evaluated for fitness, stability, and reporter gene functionality. The recombinant and parental viruses demonstrated equal fitness in terms of peak titer and replication kinetics, were stable for up to six <i>in vitro</i> passages, and were functional. Further <i>in vivo</i> evaluation indicated variable stability, but resolution limits hampered <i>in vivo</i> functional evaluation. These data support the further development of hNIS for monitoring infection in animal models of viral disease.<b>IMPORTANCE</b> Advanced medical imaging such as single photon emission computed tomography with computed tomography (SPECT/CT) enhances fields such as oncology and cardiology. Application of SPECT/CT, magnetic resonance imaging, and positron emission tomography to infectious disease may enhance pathogenesis studies and provide alternate biomarkers of disease progression. The experiments described in this article focus on insertion of a SPECT/CT-compatible reporter gene into MERS-CoV to demonstrate that a functional SPECT/CT reporter gene can be inserted into a virus.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30541777</PMID><DateCompleted><Year>2019</Year><Month>02</Month><Day>01</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>17</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2379-5042</ISSN><JournalIssue CitedMedium="Internet"><Volume>3</Volume><Issue>6</Issue><PubDate><Year>2018</Year><Month>12</Month><Day>12</Day></PubDate></JournalIssue><Title>mSphere</Title><ISOAbbreviation>mSphere</ISOAbbreviation></Journal><ArticleTitle>The Human Sodium Iodide Symporter as a Reporter Gene for Studying Middle East Respiratory Syndrome Coronavirus Pathogenesis.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">e00540-18</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1128/mSphere.00540-18</ELocationID><Abstract><AbstractText>Single photon emission computed tomography (SPECT) is frequently used in oncology and cardiology to evaluate disease progression and/or treatment efficacy. Such technology allows for real-time evaluation of disease progression and when applied to studying infectious diseases may provide insight into pathogenesis. Insertion of a SPECT-compatible reporter gene into a virus may provide insight into mechanisms of pathogenesis and viral tropism. The human sodium iodide symporter (hNIS), a SPECT and positron emission tomography reporter gene, was inserted into Middle East respiratory syndrome coronavirus (MERS-CoV), a recently emerged virus that can cause severe respiratory disease and death in afflicted humans to obtain a quantifiable and sensitive marker for viral replication to further MERS-CoV animal model development. The recombinant virus was evaluated for fitness, stability, and reporter gene functionality. The recombinant and parental viruses demonstrated equal fitness in terms of peak titer and replication kinetics, were stable for up to six <i>in vitro</i> passages, and were functional. Further <i>in vivo</i> evaluation indicated variable stability, but resolution limits hampered <i>in vivo</i> functional evaluation. These data support the further development of hNIS for monitoring infection in animal models of viral disease.<b>IMPORTANCE</b> Advanced medical imaging such as single photon emission computed tomography with computed tomography (SPECT/CT) enhances fields such as oncology and cardiology. Application of SPECT/CT, magnetic resonance imaging, and positron emission tomography to infectious disease may enhance pathogenesis studies and provide alternate biomarkers of disease progression. The experiments described in this article focus on insertion of a SPECT/CT-compatible reporter gene into MERS-CoV to demonstrate that a functional SPECT/CT reporter gene can be inserted into a virus.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chefer</LastName><ForeName>Svetlana</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Seidel</LastName><ForeName>Jurgen</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cockrell</LastName><ForeName>Adam S</ForeName><Initials>AS</Initials><AffiliationInfo><Affiliation>Department of Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yount</LastName><ForeName>Boyd</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Department of Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Solomon</LastName><ForeName>Jeffrey</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Clinical Research Directorate/Clinical Monitoring Research Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hagen</LastName><ForeName>Katie R</ForeName><Initials>KR</Initials><AffiliationInfo><Affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>David X</ForeName><Initials>DX</Initials><AffiliationInfo><Affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huzella</LastName><ForeName>Louis M</ForeName><Initials>LM</Initials><AffiliationInfo><Affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kumar</LastName><ForeName>Mia R</ForeName><Initials>MR</Initials><AffiliationInfo><Affiliation>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Postnikova</LastName><ForeName>Elena</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bohannon</LastName><ForeName>J Kyle</ForeName><Initials>JK</Initials><AffiliationInfo><Affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lackemeyer</LastName><ForeName>Matthew G</ForeName><Initials>MG</Initials><AffiliationInfo><Affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cooper</LastName><ForeName>Kurt</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Endlich-Frazier</LastName><ForeName>Ariel</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sharma</LastName><ForeName>Heema</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Thomasson</LastName><ForeName>David</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bartos</LastName><ForeName>Christopher</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sayre</LastName><ForeName>Philip J</ForeName><Initials>PJ</Initials><AffiliationInfo><Affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sims</LastName><ForeName>Amy</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dyall</LastName><ForeName>Julie</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Holbrook</LastName><ForeName>Michael R</ForeName><Initials>MR</Initials><AffiliationInfo><Affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jahrling</LastName><ForeName>Peter B</ForeName><Initials>PB</Initials><AffiliationInfo><Affiliation>Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Baric</LastName><ForeName>Ralph S</ForeName><Initials>RS</Initials><AffiliationInfo><Affiliation>Department of Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Johnson</LastName><ForeName>Reed F</ForeName><Initials>RF</Initials><AffiliationInfo><Affiliation>Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA johnsonreed@niaid.nih.gov.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>HHSN272200700016I</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R01 AI110700</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R01 AI132178</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType><PublicationType UI="D052060">Research Support, N.I.H., Intramural</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>12</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>mSphere</MedlineTA><NlmUniqueID>101674533</NlmUniqueID><ISSNLinking>2379-5042</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D027981">Symporters</NameOfSubstance></Chemical><Chemical><RegistryNumber>4XE5NDT4K1</RegistryNumber><NameOfSubstance UI="C070626">sodium-iodide symporter</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName><QualifierName UI="Q000473" MajorTopicYN="Y">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004195" MajorTopicYN="N">Disease Models, Animal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017930" MajorTopicYN="Y">Genes, Reporter</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D042822" MajorTopicYN="N">Genomic Instability</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008822" MajorTopicYN="N">Mice, Transgenic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D065207" MajorTopicYN="N">Middle East Respiratory Syndrome Coronavirus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016254" MajorTopicYN="N">Mutagenesis, Insertional</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000072098" MajorTopicYN="N">Single Photon Emission Computed Tomography Computed 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PubStatus="pubmed"><Year>2018</Year><Month>12</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>2</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30541777</ArticleId><ArticleId IdType="pii">3/6/e00540-18</ArticleId><ArticleId IdType="doi">10.1128/mSphere.00540-18</ArticleId><ArticleId IdType="pmc">PMC6291621</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Antiviral Res. 2013 Sep;99(3):207-13</Citation><ArticleIdList><ArticleId IdType="pubmed">23751367</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Curr Gene Ther. 2012 Feb 1;12(1):33-47</Citation><ArticleIdList><ArticleId IdType="pubmed">22263922</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Front Microbiol. 2012 Jan 30;3:14</Citation><ArticleIdList><ArticleId IdType="pubmed">22363320</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2015 Jul 14;112(28):8738-43</Citation><ArticleIdList><ArticleId IdType="pubmed">26124093</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Nucl Med. 2004 Mar;45(3):445-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15001685</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Microbiol. 2016 Nov 28;2:16226</Citation><ArticleIdList><ArticleId IdType="pubmed">27892925</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>mBio. 2017 Aug 22;8(4):</Citation><ArticleIdList><ArticleId IdType="pubmed">28830941</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):16157-62</Citation><ArticleIdList><ArticleId IdType="pubmed">24043791</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Vis Exp. 2014 May 15;(87):</Citation><ArticleIdList><ArticleId IdType="pubmed">24894622</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2013 Aug 1;369(5):407-16</Citation><ArticleIdList><ArticleId IdType="pubmed">23782161</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virology. 2016 Mar;490:49-58</Citation><ArticleIdList><ArticleId IdType="pubmed">26828465</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2015 Feb;89(4):2192-200</Citation><ArticleIdList><ArticleId IdType="pubmed">25473055</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2013 Oct 8;110(41):16598-603</Citation><ArticleIdList><ArticleId IdType="pubmed">24062443</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Sci Rep. 2017 Aug 17;7(1):8580</Citation><ArticleIdList><ArticleId IdType="pubmed">28819261</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Mol Cell Endocrinol. 2003 Dec 31;213(1):59-70</Citation><ArticleIdList><ArticleId IdType="pubmed">15062574</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2015 Apr;89(7):3659-70</Citation><ArticleIdList><ArticleId IdType="pubmed">25589660</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Indian J Anaesth. 2016 Feb;60(2):85-8</Citation><ArticleIdList><ArticleId IdType="pubmed">27013745</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virology. 2015 Nov;485:422-30</Citation><ArticleIdList><ArticleId IdType="pubmed">26342468</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Transl Med. 2011 Mar 31;9:36</Citation><ArticleIdList><ArticleId IdType="pubmed">21453532</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Infect Dis. 2016 May 15;213(10):1557-61</Citation><ArticleIdList><ArticleId IdType="pubmed">26941283</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol Methods. 2015 Oct;223:13-8</Citation><ArticleIdList><ArticleId IdType="pubmed">26190637</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Lancet. 2013 Jun 29;381(9885):2265-72</Citation><ArticleIdList><ArticleId IdType="pubmed">23727167</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Theranostics. 2012;2(4):392-402</Citation><ArticleIdList><ArticleId IdType="pubmed">22539935</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virus Res. 2007 Sep;128(1-2):88-98</Citation><ArticleIdList><ArticleId IdType="pubmed">17524511</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2014 Feb;88(4):2035-46</Citation><ArticleIdList><ArticleId IdType="pubmed">24307590</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virol J. 2013 Mar 25;10:95</Citation><ArticleIdList><ArticleId IdType="pubmed">23521919</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Infect Dis. 2014 Jan 15;209(2):236-42</Citation><ArticleIdList><ArticleId IdType="pubmed">24218506</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Nucl Med. 2008 Jun;49 Suppl 2:164S-79S</Citation><ArticleIdList><ArticleId IdType="pubmed">18523072</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2014 Apr 1;111(13):4970-5</Citation><ArticleIdList><ArticleId IdType="pubmed">24599590</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>EJNMMI Res. 2016 Dec;6(1):34</Citation><ArticleIdList><ArticleId IdType="pubmed">27103614</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2015 Jun;89(11):6131-5</Citation><ArticleIdList><ArticleId IdType="pubmed">25810539</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Nucl Med. 2004 Mar;45(3):500-7</Citation><ArticleIdList><ArticleId IdType="pubmed">15001694</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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