Sensitive and Specific Detection of Low-Level Antibody Responses in Mild Middle East Respiratory Syndrome Coronavirus Infections.
Identifieur interne : 000452 ( PubMed/Curation ); précédent : 000451; suivant : 000453Sensitive and Specific Detection of Low-Level Antibody Responses in Mild Middle East Respiratory Syndrome Coronavirus Infections.
Auteurs : Nisreen M A. Okba ; V Stalin Raj ; Ivy Widjaja ; Corine H. Geurtsvankessel ; Erwin De Bruin ; Felicity D. Chandler ; Wan Beom Park ; Nam-Joong Kim ; Elmoubasher A B A. Farag ; Mohammed Al-Hajri ; Berend-Jan Bosch ; Myoung-Don Oh ; Marion P G. Koopmans ; Chantal B E M. Reusken ; Bart L. HaagmansSource :
- Emerging infectious diseases [ 1080-6059 ] ; 2019.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) infections in humans can cause asymptomatic to fatal lower respiratory lung disease. Despite posing a probable risk for virus transmission, asymptomatic to mild infections can go unnoticed; a lack of seroconversion among some PCR-confirmed cases has been reported. We found that a MERS-CoV spike S1 protein-based ELISA, routinely used in surveillance studies, showed low sensitivity in detecting infections among PCR-confirmed patients with mild clinical symptoms and cross-reactivity of human coronavirus OC43-positive serum samples. Using in-house S1 ELISA and protein microarray, we demonstrate that most PCR-confirmed MERS-CoV case-patients with mild infections seroconverted; nonetheless, some of these samples did not have detectable levels of virus-neutralizing antibodies. The use of a sensitive and specific serologic S1-based assay can be instrumental in the accurate estimation of MERS-CoV prevalence.
DOI: 10.3201/eid2510.190051
PubMed: 31423970
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Okba</name></author><author><name sortKey="Raj, V Stalin" sort="Raj, V Stalin" uniqKey="Raj V" first="V Stalin" last="Raj">V Stalin Raj</name></author><author><name sortKey="Widjaja, Ivy" sort="Widjaja, Ivy" uniqKey="Widjaja I" first="Ivy" last="Widjaja">Ivy Widjaja</name></author><author><name sortKey="Geurtsvankessel, Corine H" sort="Geurtsvankessel, Corine H" uniqKey="Geurtsvankessel C" first="Corine H" last="Geurtsvankessel">Corine H. Geurtsvankessel</name></author><author><name sortKey="De Bruin, Erwin" sort="De Bruin, Erwin" uniqKey="De Bruin E" first="Erwin" last="De Bruin">Erwin De Bruin</name></author><author><name sortKey="Chandler, Felicity D" sort="Chandler, Felicity D" uniqKey="Chandler F" first="Felicity D" last="Chandler">Felicity D. Chandler</name></author><author><name sortKey="Park, Wan Beom" sort="Park, Wan Beom" uniqKey="Park W" first="Wan Beom" last="Park">Wan Beom Park</name></author><author><name sortKey="Kim, Nam Joong" sort="Kim, Nam Joong" uniqKey="Kim N" first="Nam-Joong" last="Kim">Nam-Joong Kim</name></author><author><name sortKey="Farag, Elmoubasher A B A" sort="Farag, Elmoubasher A B A" uniqKey="Farag E" first="Elmoubasher A B A" last="Farag">Elmoubasher A B A. Farag</name></author><author><name sortKey="Al Hajri, Mohammed" sort="Al Hajri, Mohammed" uniqKey="Al Hajri M" first="Mohammed" last="Al-Hajri">Mohammed Al-Hajri</name></author><author><name sortKey="Bosch, Berend Jan" sort="Bosch, Berend Jan" uniqKey="Bosch B" first="Berend-Jan" last="Bosch">Berend-Jan Bosch</name></author><author><name sortKey="Oh, Myoung Don" sort="Oh, Myoung Don" uniqKey="Oh M" first="Myoung-Don" last="Oh">Myoung-Don Oh</name></author><author><name sortKey="Koopmans, Marion P G" sort="Koopmans, Marion P G" uniqKey="Koopmans M" first="Marion P G" last="Koopmans">Marion P G. Koopmans</name></author><author><name sortKey="Reusken, Chantal B E M" sort="Reusken, Chantal B E M" uniqKey="Reusken C" first="Chantal B E M" last="Reusken">Chantal B E M. Reusken</name></author><author><name sortKey="Haagmans, Bart L" sort="Haagmans, Bart L" uniqKey="Haagmans B" first="Bart L" last="Haagmans">Bart L. Haagmans</name></author></analytic><series><title level="j">Emerging infectious diseases</title><idno type="eISSN">1080-6059</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Middle East respiratory syndrome coronavirus (MERS-CoV) infections in humans can cause asymptomatic to fatal lower respiratory lung disease. Despite posing a probable risk for virus transmission, asymptomatic to mild infections can go unnoticed; a lack of seroconversion among some PCR-confirmed cases has been reported. We found that a MERS-CoV spike S1 protein-based ELISA, routinely used in surveillance studies, showed low sensitivity in detecting infections among PCR-confirmed patients with mild clinical symptoms and cross-reactivity of human coronavirus OC43-positive serum samples. Using in-house S1 ELISA and protein microarray, we demonstrate that most PCR-confirmed MERS-CoV case-patients with mild infections seroconverted; nonetheless, some of these samples did not have detectable levels of virus-neutralizing antibodies. The use of a sensitive and specific serologic S1-based assay can be instrumental in the accurate estimation of MERS-CoV prevalence.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">31423970</PMID><DateRevised><Year>2020</Year><Month>03</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1080-6059</ISSN><JournalIssue CitedMedium="Internet"><Volume>25</Volume><Issue>10</Issue><PubDate><Year>2019</Year><Month>10</Month></PubDate></JournalIssue><Title>Emerging infectious diseases</Title><ISOAbbreviation>Emerging Infect. Dis.</ISOAbbreviation></Journal><ArticleTitle>Sensitive and Specific Detection of Low-Level Antibody Responses in Mild Middle East Respiratory Syndrome Coronavirus Infections.</ArticleTitle><Pagination><MedlinePgn>1868-1877</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3201/eid2510.190051</ELocationID><Abstract><AbstractText>Middle East respiratory syndrome coronavirus (MERS-CoV) infections in humans can cause asymptomatic to fatal lower respiratory lung disease. Despite posing a probable risk for virus transmission, asymptomatic to mild infections can go unnoticed; a lack of seroconversion among some PCR-confirmed cases has been reported. We found that a MERS-CoV spike S1 protein-based ELISA, routinely used in surveillance studies, showed low sensitivity in detecting infections among PCR-confirmed patients with mild clinical symptoms and cross-reactivity of human coronavirus OC43-positive serum samples. Using in-house S1 ELISA and protein microarray, we demonstrate that most PCR-confirmed MERS-CoV case-patients with mild infections seroconverted; nonetheless, some of these samples did not have detectable levels of virus-neutralizing antibodies. The use of a sensitive and specific serologic S1-based assay can be instrumental in the accurate estimation of MERS-CoV prevalence.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Okba</LastName><ForeName>Nisreen M A</ForeName><Initials>NMA</Initials></Author><Author ValidYN="Y"><LastName>Raj</LastName><ForeName>V Stalin</ForeName><Initials>VS</Initials></Author><Author ValidYN="Y"><LastName>Widjaja</LastName><ForeName>Ivy</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>GeurtsvanKessel</LastName><ForeName>Corine H</ForeName><Initials>CH</Initials></Author><Author ValidYN="Y"><LastName>de Bruin</LastName><ForeName>Erwin</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Chandler</LastName><ForeName>Felicity D</ForeName><Initials>FD</Initials></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Wan Beom</ForeName><Initials>WB</Initials></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Nam-Joong</ForeName><Initials>NJ</Initials></Author><Author ValidYN="Y"><LastName>Farag</LastName><ForeName>Elmoubasher A B A</ForeName><Initials>EABA</Initials></Author><Author ValidYN="Y"><LastName>Al-Hajri</LastName><ForeName>Mohammed</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Bosch</LastName><ForeName>Berend-Jan</ForeName><Initials>BJ</Initials></Author><Author ValidYN="Y"><LastName>Oh</LastName><ForeName>Myoung-Don</ForeName><Initials>MD</Initials></Author><Author ValidYN="Y"><LastName>Koopmans</LastName><ForeName>Marion P G</ForeName><Initials>MPG</Initials></Author><Author ValidYN="Y"><LastName>Reusken</LastName><ForeName>Chantal B E M</ForeName><Initials>CBEM</Initials></Author><Author ValidYN="Y"><LastName>Haagmans</LastName><ForeName>Bart L</ForeName><Initials>BL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal 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