Recombinant adenoviral vaccine encoding the spike 1 subunit of the Middle East Respiratory Syndrome Coronavirus elicits strong humoral and cellular immune responses in mice.
Identifieur interne : 000325 ( PubMed/Curation ); précédent : 000324; suivant : 000326Recombinant adenoviral vaccine encoding the spike 1 subunit of the Middle East Respiratory Syndrome Coronavirus elicits strong humoral and cellular immune responses in mice.
Auteurs : Mustafa Ababneh [Jordanie] ; Mu'Men Alrwashdeh [Jordanie] ; Mohammad Khalifeh [Jordanie]Source :
- Veterinary world [ 0972-8988 ] ; 2019.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) has rapidly spread throughout the Middle East since its discovery in 2012. The virus poses a significant global public health threat with potentially devastating effects. In this study, a recombinant adenoviral-based vaccine encoding the spike 1 (S1) subunit of the MERS-CoV genome was constructed, and its humoral, and cellular immune responses were evaluated in mice.
DOI: 10.14202/vetworld.2019.1554-1562
PubMed: 31849416
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elicits strong humoral and cellular immune responses in mice.</title>
<author><name sortKey="Ababneh, Mustafa" sort="Ababneh, Mustafa" uniqKey="Ababneh M" first="Mustafa" last="Ababneh">Mustafa Ababneh</name>
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elicits strong humoral and cellular immune responses in mice.</title>
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<front><div type="abstract" xml:lang="en"><i>Middle East respiratory syndrome coronavirus</i>
(MERS-CoV) has rapidly spread throughout the Middle East since its discovery in 2012. The virus poses a significant global public health threat with potentially devastating effects. In this study, a recombinant adenoviral-based vaccine encoding the spike 1 (S1) subunit of the MERS-CoV genome was constructed, and its humoral, and cellular immune responses were evaluated in mice.</div>
</front>
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<DateRevised><Year>2019</Year>
<Month>12</Month>
<Day>20</Day>
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<Issue>10</Issue>
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<Month>Oct</Month>
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<Title>Veterinary world</Title>
<ISOAbbreviation>Vet World</ISOAbbreviation>
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<ArticleTitle>Recombinant adenoviral vaccine encoding the spike 1 subunit of the <i>Middle East Respiratory Syndrome Coronavirus</i>
elicits strong humoral and cellular immune responses in mice.</ArticleTitle>
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<Abstract><AbstractText Label="Background and Aim" NlmCategory="UNASSIGNED"><i>Middle East respiratory syndrome coronavirus</i>
(MERS-CoV) has rapidly spread throughout the Middle East since its discovery in 2012. The virus poses a significant global public health threat with potentially devastating effects. In this study, a recombinant adenoviral-based vaccine encoding the spike 1 (S1) subunit of the MERS-CoV genome was constructed, and its humoral, and cellular immune responses were evaluated in mice.</AbstractText>
<AbstractText Label="Materials and Methods" NlmCategory="UNASSIGNED">Mice were immunized initially by intramuscular injection and boosted 3 weeks later by intranasal application. Expression of the S1 protein in the lungs and kidneys was detected using conventional polymerase chain reaction (PCR) and immunohistochemistry (IHC) targeting specific regions within the S1 subunit at weeks 3, 4, 5, and 6 after the first vaccination. Antigen-specific humoral and cellular immune responses were evaluated in serum and in cell culture following <i>in vitro</i>
stimulation with a specific 9-mer epitope within the S1 protein (CYSSLILDY).</AbstractText>
<AbstractText Label="Results" NlmCategory="UNASSIGNED">S1 protein expression was only detected by IHC in the kidneys of the Ad-MERS-S1 group at week 6 from first immunization, and in both lungs and kidneys of Ad-MERS-S1 group by conventional PCR at weeks 3 and 5 post-prime. The vaccine elicited a specific S1-immunoglobulin G antibody response, which was detected in the sera of the vaccinated mice at weeks 4 and 6 from the onset of the first immunization. There was a significant increase in the amount of Th1-related cytokines (interferon-γ and interleukin [IL] 12), and a significant decrease in the Th2-related cytokine IL-4 in splenocyte cell culture of the vaccinated group compared with the control groups.</AbstractText>
<AbstractText Label="Conclusion" NlmCategory="UNASSIGNED">The results of this study suggest that this recombinant adenovirus vaccine encoding the S1 subunit of MERS-CoV elicits potentially protective antigen-specific humoral and cellular immune responses in mice. This study demonstrates a promising vaccine for the control and/or prevention of MERS-CoV infection in humans.</AbstractText>
<CopyrightInformation>Copyright: © Ababneh, et al.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ababneh</LastName>
<ForeName>Mustafa</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Department of Basic Medical Veterinary Sciences, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan.</Affiliation>
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<Author ValidYN="Y"><LastName>Alrwashdeh</LastName>
<ForeName>Mu'men</ForeName>
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<AffiliationInfo><Affiliation>Department of Basic Medical Veterinary Sciences, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan.</Affiliation>
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<AffiliationInfo><Affiliation>Department of Basic Medical Veterinary Sciences, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan.</Affiliation>
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<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Middle East respiratory syndrome</Keyword>
<Keyword MajorTopicYN="N">coronavirus</Keyword>
<Keyword MajorTopicYN="N">recombinant vaccine</Keyword>
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</ArticleIdList>
<ReferenceList><Reference><Citation>Vaccine. 2014 Oct 14;32(45):5975-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25192975</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virus Res. 2005 Sep;112(1-2):24-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16022898</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2005 May;79(10):6516-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15858035</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Infect Dis. 2019 Oct 8;220(10):1558-1567</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30911758</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Infect Immun. 2004 Apr;72(4):1974-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15039317</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Vaccine. 2014 Apr 11;32(18):2100-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24560617</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS One. 2013 Dec 04;8(12):e81587</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24324708</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Paediatr Respir Rev. 2015 Jun;16(3):197-202</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26002405</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biologicals. 2018 Jul;54:58-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29759890</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Pak Med Assoc. 2013 Nov;63(11):1329-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24392512</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Lancet Infect Dis. 2014 Feb;14(2):140-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24355866</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Protein Cell. 2013 Dec;4(12):951-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24318862</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2013 Nov;87(21):11950-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23986586</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Can Urol Assoc J. 2014 Jul;8(7-8):268-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25210551</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Gen Virol. 2013 Dec;94(Pt 12):2679-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24077366</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Bioinformation. 2014 Aug 30;10(8):533-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25258490</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Emerg Infect Dis. 2014 Apr;20(4):552-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24655412</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Vaccine. 2017 Jun 27;35(30):3780-3788</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28579232</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>N Engl J Med. 2012 Nov 8;367(19):1814-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23075143</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Vaccine. 2006 Jan 16;24(3):355-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16153755</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Vaccine. 2018 Jun 7;36(24):3468-3476</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29739720</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Acta Naturae. 2019 Jan-Mar;11(1):38-47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">31024747</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Immunology. 2015 Aug;145(4):476-84</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25762305</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>MBio. 2012 Nov 20;3(6):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23170002</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Curr Opin Virol. 2014 Apr;5:58-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24584035</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nature. 2013 Mar 14;495(7440):251-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23486063</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2015 Aug;89(16):8651-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26018172</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2013 Jun;87(12):6604-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23552422</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Altern Med Rev. 2003 Aug;8(3):223-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12946237</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>MBio. 2014 Feb 25;5(2):e00884-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24570370</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS One. 2014 Feb 14;9(2):e88716</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24551142</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Euro Surveill. 2013 Dec 12;18(50):20662</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24342516</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Formos Med Assoc. 2014 Mar;113(3):143-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24342026</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>NPJ Vaccines. 2017 Oct 16;2:28</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29263883</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Cell Res. 2013 Aug;23(8):986-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23835475</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Histopathology. 1997 Nov;31(5):400-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9416479</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Methods. 2001 Dec;25(4):402-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11846609</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Trends Microbiol. 2014 Oct;22(10):573-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25178651</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Gen Virol. 2006 Mar;87(Pt 3):641-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16476986</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virol J. 2013 Dec 23;10:359</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24364985</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Crit Rev Clin Lab Sci. 2003 Jun;40(3):209-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12892317</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Gen Virol. 2008 Sep;89(Pt 9):2136-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18753223</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Mol Ther. 2004 Oct;10(4):616-29</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15451446</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>MBio. 2013 Apr 30;4(3):e00165-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23631916</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Emerg Microbes Infect. 2019;8(1):760-772</Citation>
<ArticleIdList><ArticleId IdType="pubmed">31130102</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>MBio. 2014 Jul 22;5(4):e01450-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25053787</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Euro Surveill. 2013 Dec 12;18(50):20659</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24342517</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>
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