Polyribosomes from Peas: II. Polyribosome Metabolism during Normal and Hormone-induced Growth.
Identifieur interne : 002B20 ( PubMed/Corpus ); précédent : 002B19; suivant : 002B21Polyribosomes from Peas: II. Polyribosome Metabolism during Normal and Hormone-induced Growth.
Auteurs : E. Davies ; B A LarkinsSource :
- Plant physiology [ 0032-0889 ] ; 1973.
Abstract
Polyribosomes as large as 10-mers (strands of messenger RNA bearing 10 ribosomes) were isolated from etiolated pea (Pisum sativum L. var. Alaska) stem tissue during all stages of development when methods were used which essentially eliminated ribonuclease activity during extraction. Actively growing tissue, harvested from the apical 10 mm, yielded many large polyribosomes and a low (<20%) proportion of monosomes. Similar tissue, allowed to age by applying lanolin to decapitated apices, showed a progressive decrease in number of larger polyribosomes and an increase in the proportion of monosomes. Hormone treatments, which prolonged growth and delayed aging, delayed the loss in large polyribosomes and the increase in proportion of monosomes. Growth-stimulating hormones, added to previously aged tissue, stimulated the production of many large polyribosomes in pre-existing cells.It is suggested that (a) large polyribosomes occur in all regions of the pea stem, (b) changes in polyribosome distribution appear to precede changes in growth rate, (c) loss of larger polyribosomes is closely related to a decrease in mRNA templates followed more gradually by loss of ribosomes, (d) hormone-stimulated continuation of growth is accomplished through maintenance of available mRNA.Methods are described, involving detailed analysis of polysome distribution, which, although they cannot be used to measure changes in initiation of ribosomes on to mRNA, do permit measurement of the amount of polysomal-associated mRNA present in tissues at different stages of growth. These analyses lead to the further suggestion that hormone stimulation of growth of previously nongrowing tissue is accomplished primarily through an increase in available mRNA prior to synthesis of ribosomes.
DOI: 10.1104/pp.52.4.339
PubMed: 16658559
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Davies</name><affiliation><nlm:affiliation>Department of Botany, University of Nebraska, Lincoln, Nebraska, 68508.</nlm:affiliation></affiliation></author><author><name sortKey="Larkins, B A" sort="Larkins, B A" uniqKey="Larkins B" first="B A" last="Larkins">B A Larkins</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1973">1973</date><idno type="RBID">pubmed:16658559</idno><idno type="pmid">16658559</idno><idno type="doi">10.1104/pp.52.4.339</idno><idno type="wicri:Area/PubMed/Corpus">002B20</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002B20</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Polyribosomes from Peas: II. Polyribosome Metabolism during Normal and Hormone-induced Growth.</title><author><name sortKey="Davies, E" sort="Davies, E" uniqKey="Davies E" first="E" last="Davies">E. Davies</name><affiliation><nlm:affiliation>Department of Botany, University of Nebraska, Lincoln, Nebraska, 68508.</nlm:affiliation></affiliation></author><author><name sortKey="Larkins, B A" sort="Larkins, B A" uniqKey="Larkins B" first="B A" last="Larkins">B A Larkins</name></author></analytic><series><title level="j">Plant physiology</title><idno type="ISSN">0032-0889</idno><imprint><date when="1973" type="published">1973</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Polyribosomes as large as 10-mers (strands of messenger RNA bearing 10 ribosomes) were isolated from etiolated pea (Pisum sativum L. var. Alaska) stem tissue during all stages of development when methods were used which essentially eliminated ribonuclease activity during extraction. Actively growing tissue, harvested from the apical 10 mm, yielded many large polyribosomes and a low (<20%) proportion of monosomes. Similar tissue, allowed to age by applying lanolin to decapitated apices, showed a progressive decrease in number of larger polyribosomes and an increase in the proportion of monosomes. Hormone treatments, which prolonged growth and delayed aging, delayed the loss in large polyribosomes and the increase in proportion of monosomes. Growth-stimulating hormones, added to previously aged tissue, stimulated the production of many large polyribosomes in pre-existing cells.It is suggested that (a) large polyribosomes occur in all regions of the pea stem, (b) changes in polyribosome distribution appear to precede changes in growth rate, (c) loss of larger polyribosomes is closely related to a decrease in mRNA templates followed more gradually by loss of ribosomes, (d) hormone-stimulated continuation of growth is accomplished through maintenance of available mRNA.Methods are described, involving detailed analysis of polysome distribution, which, although they cannot be used to measure changes in initiation of ribosomes on to mRNA, do permit measurement of the amount of polysomal-associated mRNA present in tissues at different stages of growth. These analyses lead to the further suggestion that hormone stimulation of growth of previously nongrowing tissue is accomplished primarily through an increase in available mRNA prior to synthesis of ribosomes.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">16658559</PMID><DateCompleted><Year>2010</Year><Month>06</Month><Day>29</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>14</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0032-0889</ISSN><JournalIssue CitedMedium="Print"><Volume>52</Volume><Issue>4</Issue><PubDate><Year>1973</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol.</ISOAbbreviation></Journal><ArticleTitle>Polyribosomes from Peas: II. Polyribosome Metabolism during Normal and Hormone-induced Growth.</ArticleTitle><Pagination><MedlinePgn>339-45</MedlinePgn></Pagination><Abstract><AbstractText>Polyribosomes as large as 10-mers (strands of messenger RNA bearing 10 ribosomes) were isolated from etiolated pea (Pisum sativum L. var. Alaska) stem tissue during all stages of development when methods were used which essentially eliminated ribonuclease activity during extraction. Actively growing tissue, harvested from the apical 10 mm, yielded many large polyribosomes and a low (<20%) proportion of monosomes. Similar tissue, allowed to age by applying lanolin to decapitated apices, showed a progressive decrease in number of larger polyribosomes and an increase in the proportion of monosomes. Hormone treatments, which prolonged growth and delayed aging, delayed the loss in large polyribosomes and the increase in proportion of monosomes. Growth-stimulating hormones, added to previously aged tissue, stimulated the production of many large polyribosomes in pre-existing cells.It is suggested that (a) large polyribosomes occur in all regions of the pea stem, (b) changes in polyribosome distribution appear to precede changes in growth rate, (c) loss of larger polyribosomes is closely related to a decrease in mRNA templates followed more gradually by loss of ribosomes, (d) hormone-stimulated continuation of growth is accomplished through maintenance of available mRNA.Methods are described, involving detailed analysis of polysome distribution, which, although they cannot be used to measure changes in initiation of ribosomes on to mRNA, do permit measurement of the amount of polysomal-associated mRNA present in tissues at different stages of growth. These analyses lead to the further suggestion that hormone stimulation of growth of previously nongrowing tissue is accomplished primarily through an increase in available mRNA prior to synthesis of ribosomes.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Davies</LastName><ForeName>E</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department of Botany, University of Nebraska, Lincoln, Nebraska, 68508.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Larkins</LastName><ForeName>B A</ForeName><Initials>BA</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Physiol</MedlineTA><NlmUniqueID>0401224</NlmUniqueID><ISSNLinking>0032-0889</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1973</Year><Month>10</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1973</Year><Month>10</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1973</Year><Month>10</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16658559</ArticleId><ArticleId IdType="pmc">PMC366499</ArticleId><ArticleId IdType="doi">10.1104/pp.52.4.339</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Cell Sci. 1971 May;8(3):649-58</Citation><ArticleIdList><ArticleId IdType="pubmed">4103449</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 1972 Nov 9;281(4):571-96</Citation><ArticleIdList><ArticleId IdType="pubmed">4631639</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Biochem. 1972 Apr;46(2):365-73</Citation><ArticleIdList><ArticleId IdType="pubmed">5024612</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1964 Jan 18;201:264-70</Citation><ArticleIdList><ArticleId IdType="pubmed">14110449</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1967 Aug;42(8):1114-22</Citation><ArticleIdList><ArticleId IdType="pubmed">16656623</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1972 Mar;49(3):371-5</Citation><ArticleIdList><ArticleId IdType="pubmed">16657963</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1972 Nov;50(5):581-4</Citation><ArticleIdList><ArticleId IdType="pubmed">16658221</ArticleId></ArticleIdList></Reference><Reference><Citation>Exp Cell Res. 1972 Jun;72(2):431-5</Citation><ArticleIdList><ArticleId IdType="pubmed">5037928</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 1971 Oct;247(3):471-85</Citation><ArticleIdList><ArticleId IdType="pubmed">5125631</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Biochem. 1971 Sep;43(1):71-9</Citation><ArticleIdList><ArticleId IdType="pubmed">5130413</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 1971 Oct;247(3):468-70</Citation><ArticleIdList><ArticleId IdType="pubmed">5315987</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem J. 1967 Jan;102(1):251-7</Citation><ArticleIdList><ArticleId IdType="pubmed">5339944</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Biochem Biophys. 1970 Apr;137(2):442-52</Citation><ArticleIdList><ArticleId IdType="pubmed">5439306</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 1970 Nov 12;224(1):155-64</Citation><ArticleIdList><ArticleId IdType="pubmed">5490250</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Biochem Biophys. 1968 Dec;128(3):595-600</Citation><ArticleIdList><ArticleId IdType="pubmed">5704297</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Biochem Biophys. 1969 Feb;129(2):581-7</Citation><ArticleIdList><ArticleId IdType="pubmed">5772969</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 1967 Jun 14;26(2):211-25</Citation><ArticleIdList><ArticleId IdType="pubmed">6034773</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1963 Feb 2;197:430-5</Citation><ArticleIdList><ArticleId IdType="pubmed">14000153</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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