MersV1, PubMed, Corpus, bibRecord, 002A19

A novel protein binds a key origin sequence to block replication of an E. coli minichromosome.

Identifieur interne : 002A19 ( PubMed/Corpus ); précédent : 002A18; suivant : 002A20

A novel protein binds a key origin sequence to block replication of an E. coli minichromosome.

Auteurs : D S Hwang ; A. Kornberg

Source :

Cell [ 0092-8674 ] ; 1990.

RBID : pubmed:2208289

English descriptors

KwdEn :
- Base Sequence, Chromatography, Affinity, Chromatography, Ion Exchange, Chromosomes, Bacterial, DNA Replication, DNA, Bacterial (genetics), DNA-Binding Proteins (isolation & purification), DNA-Binding Proteins (metabolism), Deoxyribonuclease I, Escherichia coli (genetics), Escherichia coli (metabolism), Kinetics, Molecular Sequence Data, Molecular Weight, Oligonucleotide Probes, Plasmids, Restriction Mapping.
MESH :
- chemical , genetics : DNA, Bacterial.
- chemical , isolation & purification : DNA-Binding Proteins.
- chemical , metabolism : DNA-Binding Proteins.
- genetics : Escherichia coli.
- metabolism : Escherichia coli.
- Base Sequence, Chromatography, Affinity, Chromatography, Ion Exchange, Chromosomes, Bacterial, DNA Replication, Deoxyribonuclease I, Kinetics, Molecular Sequence Data, Molecular Weight, Oligonucleotide Probes, Plasmids, Restriction Mapping.

Abstract

A sequence of three tandem repeats of a 13-mer in the replication origin (oriC) of E. coli is the highly conserved site of opening of the duplex for initiation of DNA synthesis. A protein that binds this sequence has been discovered in E. coli and purified to homogeneity. This novel 33 kd polypeptide behaves as a dimer. Binding to the 13-mers is specific and limited to this region. At a ratio of 10-20 monomers per oriC plasmid, the binding blocks initiation by preventing the opening of the 13-mer region by dnaA protein. Once the 13-mers are opened by dnaA protein action, the 33 kd protein is without effect on the subsequent stages of replication. The specificity of binding and profound inhibitory effect suggest a regulatory role for this protein at an early stage of chromosome initiation.

DOI: 10.1016/0092-8674(90)90165-b
PubMed: 2208289

Links to Exploration step

pubmed:2208289

Le document en format XML

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<author><name sortKey="Hwang, D S" sort="Hwang, D S" uniqKey="Hwang D" first="D S" last="Hwang">D S Hwang</name>
<affiliation><nlm:affiliation>Department of Biochemistry, Stanford University School of Medicine, California 94305-5307.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Kornberg, A" sort="Kornberg, A" uniqKey="Kornberg A" first="A" last="Kornberg">A. Kornberg</name>
</author>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">A novel protein binds a key origin sequence to block replication of an E. coli minichromosome.</title>
<author><name sortKey="Hwang, D S" sort="Hwang, D S" uniqKey="Hwang D" first="D S" last="Hwang">D S Hwang</name>
<affiliation><nlm:affiliation>Department of Biochemistry, Stanford University School of Medicine, California 94305-5307.</nlm:affiliation>
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</author>
<author><name sortKey="Kornberg, A" sort="Kornberg, A" uniqKey="Kornberg A" first="A" last="Kornberg">A. Kornberg</name>
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<series><title level="j">Cell</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term>
<term>Chromatography, Affinity</term>
<term>Chromatography, Ion Exchange</term>
<term>Chromosomes, Bacterial</term>
<term>DNA Replication</term>
<term>DNA, Bacterial (genetics)</term>
<term>DNA-Binding Proteins (isolation & purification)</term>
<term>DNA-Binding Proteins (metabolism)</term>
<term>Deoxyribonuclease I</term>
<term>Escherichia coli (genetics)</term>
<term>Escherichia coli (metabolism)</term>
<term>Kinetics</term>
<term>Molecular Sequence Data</term>
<term>Molecular Weight</term>
<term>Oligonucleotide Probes</term>
<term>Plasmids</term>
<term>Restriction Mapping</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA-Binding Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA-Binding Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term>
<term>Chromatography, Affinity</term>
<term>Chromatography, Ion Exchange</term>
<term>Chromosomes, Bacterial</term>
<term>DNA Replication</term>
<term>Deoxyribonuclease I</term>
<term>Kinetics</term>
<term>Molecular Sequence Data</term>
<term>Molecular Weight</term>
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<front><div type="abstract" xml:lang="en">A sequence of three tandem repeats of a 13-mer in the replication origin (oriC) of E. coli is the highly conserved site of opening of the duplex for initiation of DNA synthesis. A protein that binds this sequence has been discovered in E. coli and purified to homogeneity. This novel 33 kd polypeptide behaves as a dimer. Binding to the 13-mers is specific and limited to this region. At a ratio of 10-20 monomers per oriC plasmid, the binding blocks initiation by preventing the opening of the 13-mer region by dnaA protein. Once the 13-mers are opened by dnaA protein action, the 33 kd protein is without effect on the subsequent stages of replication. The specificity of binding and profound inhibitory effect suggest a regulatory role for this protein at an early stage of chromosome initiation.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">2208289</PMID>
<DateCompleted><Year>1990</Year>
<Month>11</Month>
<Day>19</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>07</Month>
<Day>05</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0092-8674</ISSN>
<JournalIssue CitedMedium="Print"><Volume>63</Volume>
<Issue>2</Issue>
<PubDate><Year>1990</Year>
<Month>Oct</Month>
<Day>19</Day>
</PubDate>
</JournalIssue>
<Title>Cell</Title>
<ISOAbbreviation>Cell</ISOAbbreviation>
</Journal>
<ArticleTitle>A novel protein binds a key origin sequence to block replication of an E. coli minichromosome.</ArticleTitle>
<Pagination><MedlinePgn>325-31</MedlinePgn>
</Pagination>
<Abstract><AbstractText>A sequence of three tandem repeats of a 13-mer in the replication origin (oriC) of E. coli is the highly conserved site of opening of the duplex for initiation of DNA synthesis. A protein that binds this sequence has been discovered in E. coli and purified to homogeneity. This novel 33 kd polypeptide behaves as a dimer. Binding to the 13-mers is specific and limited to this region. At a ratio of 10-20 monomers per oriC plasmid, the binding blocks initiation by preventing the opening of the 13-mer region by dnaA protein. Once the 13-mers are opened by dnaA protein action, the 33 kd protein is without effect on the subsequent stages of replication. The specificity of binding and profound inhibitory effect suggest a regulatory role for this protein at an early stage of chromosome initiation.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hwang</LastName>
<ForeName>D S</ForeName>
<Initials>DS</Initials>
<AffiliationInfo><Affiliation>Department of Biochemistry, Stanford University School of Medicine, California 94305-5307.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kornberg</LastName>
<ForeName>A</ForeName>
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<Language>eng</Language>
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<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Cell</MedlineTA>
<NlmUniqueID>0413066</NlmUniqueID>
<ISSNLinking>0092-8674</ISSNLinking>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
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<Chemical><RegistryNumber>0</RegistryNumber>
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<Chemical><RegistryNumber>EC 3.1.21.1</RegistryNumber>
<NameOfSubstance UI="D003850">Deoxyribonuclease I</NameOfSubstance>
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<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName>
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<MeshHeading><DescriptorName UI="D002846" MajorTopicYN="N">Chromatography, Affinity</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002852" MajorTopicYN="N">Chromatography, Ion Exchange</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002876" MajorTopicYN="Y">Chromosomes, Bacterial</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004261" MajorTopicYN="Y">DNA Replication</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004269" MajorTopicYN="N">DNA, Bacterial</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004268" MajorTopicYN="N">DNA-Binding Proteins</DescriptorName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003850" MajorTopicYN="N">Deoxyribonuclease I</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008970" MajorTopicYN="N">Molecular Weight</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015345" MajorTopicYN="N">Oligonucleotide Probes</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010957" MajorTopicYN="Y">Plasmids</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015183" MajorTopicYN="N">Restriction Mapping</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1990</Year>
<Month>10</Month>
<Day>19</Day>
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A novel protein binds a key origin sequence to block replication of an E. coli minichromosome.

A novel protein binds a key origin sequence to block replication of an E. coli minichromosome.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki