The Immobiline family: from "vacuum" to "plenum" chemistry.
Identifieur interne : 002961 ( PubMed/Corpus ); précédent : 002960; suivant : 002962The Immobiline family: from "vacuum" to "plenum" chemistry.
Auteurs : M. Chiari ; P G RighettiSource :
- Electrophoresis [ 0173-0835 ] ; 1992.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Acids, Acrylamides, Alkalies.
- methods : Electrophoresis.
- Hydrolysis, Isoelectric Focusing, Molecular Structure.
Abstract
We list here a total of 17 acrylamido acids and bases as potential buffers and titrants for isoelectric focusing separations in immobilized pH gradients. The chemistry of these compounds is reviewed and general guidelines are given for their proper use. In particular, it is shown that the most delicate compounds are the basic species, since they can undergo several degradation pathways, including: (i) spontaneous hydrolysis to acrylic acid and a diamine; (ii) spontaneous autopolymerization to oligomers and n-mers; (iii) oxidation to N-oxides during the persulfate polymerization step. A hydrophobicity scale has been constructed, by partitioning the deprotonated species in water/1-octanol phases. A scale of resistance to alkaline hydrolysis for the basic acrylamido buffers is also given, followed by general consideration on the structure/stability relationship of these chemicals.
DOI: 10.1002/elps.1150130140
PubMed: 1628597
Links to Exploration step
pubmed:1628597Le document en format XML
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Chiari</name><affiliation><nlm:affiliation>Chair of Biochemistry, University of Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Righetti, P G" sort="Righetti, P G" uniqKey="Righetti P" first="P G" last="Righetti">P G Righetti</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1992">1992</date><idno type="RBID">pubmed:1628597</idno><idno type="pmid">1628597</idno><idno type="doi">10.1002/elps.1150130140</idno><idno type="wicri:Area/PubMed/Corpus">002961</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002961</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The Immobiline family: from "vacuum" to "plenum" chemistry.</title><author><name sortKey="Chiari, M" sort="Chiari, M" uniqKey="Chiari M" first="M" last="Chiari">M. Chiari</name><affiliation><nlm:affiliation>Chair of Biochemistry, University of Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Righetti, P G" sort="Righetti, P G" uniqKey="Righetti P" first="P G" last="Righetti">P G Righetti</name></author></analytic><series><title level="j">Electrophoresis</title><idno type="ISSN">0173-0835</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acids (chemistry)</term><term>Acrylamides (chemistry)</term><term>Alkalies (chemistry)</term><term>Electrophoresis (methods)</term><term>Hydrolysis</term><term>Isoelectric Focusing</term><term>Molecular Structure</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Acids</term><term>Acrylamides</term><term>Alkalies</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Electrophoresis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Hydrolysis</term><term>Isoelectric Focusing</term><term>Molecular Structure</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We list here a total of 17 acrylamido acids and bases as potential buffers and titrants for isoelectric focusing separations in immobilized pH gradients. The chemistry of these compounds is reviewed and general guidelines are given for their proper use. In particular, it is shown that the most delicate compounds are the basic species, since they can undergo several degradation pathways, including: (i) spontaneous hydrolysis to acrylic acid and a diamine; (ii) spontaneous autopolymerization to oligomers and n-mers; (iii) oxidation to N-oxides during the persulfate polymerization step. A hydrophobicity scale has been constructed, by partitioning the deprotonated species in water/1-octanol phases. A scale of resistance to alkaline hydrolysis for the basic acrylamido buffers is also given, followed by general consideration on the structure/stability relationship of these chemicals.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">1628597</PMID><DateCompleted><Year>1992</Year><Month>08</Month><Day>20</Day></DateCompleted><DateRevised><Year>2019</Year><Month>10</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0173-0835</ISSN><JournalIssue CitedMedium="Print"><Volume>13</Volume><Issue>4</Issue><PubDate><Year>1992</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Electrophoresis</Title><ISOAbbreviation>Electrophoresis</ISOAbbreviation></Journal><ArticleTitle>The Immobiline family: from "vacuum" to "plenum" chemistry.</ArticleTitle><Pagination><MedlinePgn>187-91</MedlinePgn></Pagination><Abstract><AbstractText>We list here a total of 17 acrylamido acids and bases as potential buffers and titrants for isoelectric focusing separations in immobilized pH gradients. The chemistry of these compounds is reviewed and general guidelines are given for their proper use. In particular, it is shown that the most delicate compounds are the basic species, since they can undergo several degradation pathways, including: (i) spontaneous hydrolysis to acrylic acid and a diamine; (ii) spontaneous autopolymerization to oligomers and n-mers; (iii) oxidation to N-oxides during the persulfate polymerization step. A hydrophobicity scale has been constructed, by partitioning the deprotonated species in water/1-octanol phases. A scale of resistance to alkaline hydrolysis for the basic acrylamido buffers is also given, followed by general consideration on the structure/stability relationship of these chemicals.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chiari</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Chair of Biochemistry, University of Milano, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Righetti</LastName><ForeName>P G</ForeName><Initials>PG</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Electrophoresis</MedlineTA><NlmUniqueID>8204476</NlmUniqueID><ISSNLinking>0173-0835</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000143">Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000178">Acrylamides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000468">Alkalies</NameOfSubstance></Chemical><Chemical><RegistryNumber>88385-55-1</RegistryNumber><NameOfSubstance UI="C059413">Immobiline</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000143" MajorTopicYN="N">Acids</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000178" MajorTopicYN="N">Acrylamides</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000468" MajorTopicYN="N">Alkalies</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004586" MajorTopicYN="N">Electrophoresis</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007525" MajorTopicYN="Y">Isoelectric Focusing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015394" MajorTopicYN="N">Molecular Structure</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1992</Year><Month>4</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1992</Year><Month>4</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1992</Year><Month>4</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">1628597</ArticleId><ArticleId IdType="doi">10.1002/elps.1150130140</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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