MersV1, PubMed, Corpus, bibRecord, 002958

A novel blocker-PCR method for detection of rare mutant alleles in the presence of an excess amount of normal DNA.

Identifieur interne : 002958 ( PubMed/Corpus ); précédent : 002957; suivant : 002959

A novel blocker-PCR method for detection of rare mutant alleles in the presence of an excess amount of normal DNA.

Auteurs : T. Seyama ; T. Ito ; T. Hayashi ; T. Mizuno ; N. Nakamura ; M. Akiyama

Source :

Nucleic acids research [ 0305-1048 ] ; 1992.

RBID : pubmed:1598207

English descriptors

KwdEn :
- Alleles, Base Sequence, DNA, Single-Stranded (genetics), Genes, ras (genetics), Humans, Molecular Sequence Data, Mutation (genetics), Nucleic Acid Conformation, Oligodeoxyribonucleotides (genetics), Polymerase Chain Reaction (methods), Polymorphism, Genetic (genetics), Sensitivity and Specificity, Temperature.
MESH :
- chemical , genetics : DNA, Single-Stranded, Oligodeoxyribonucleotides.
- genetics : Genes, ras, Mutation, Polymorphism, Genetic.
- methods : Polymerase Chain Reaction.
- Alleles, Base Sequence, Humans, Molecular Sequence Data, Nucleic Acid Conformation, Sensitivity and Specificity, Temperature.

Abstract

A novel polymerase chain reaction method was developed to preferentially amplify a segment of DNA containing a base substitution mutation. This technique uses a pair of dideoxynucleotide-labeled oligonucleotides (18 mers) of normal sequences as blockers located between the two primers. By virtue of a subtle difference in the melting temperature between the blocker-normal DNA and blocker-mutant DNA hybrids, the method allows preferential amplification of the mutant DNA. We used the human N-ras gene as a model. Two different types of N-ras mutations could be effectively amplified when they were present with an excess amount of normal DNA at a ratio of 1:10(3). Furthermore, the sensitivity was increased 10-fold by using single strand conformation polymorphism analysis for the amplified products, and mutant DNA was detected in the presence of a 10(4) times excess amount of normal DNA.

DOI: 10.1093/nar/20.10.2493
PubMed: 1598207

Links to Exploration step

pubmed:1598207

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A novel blocker-PCR method for detection of rare mutant alleles in the presence of an excess amount of normal DNA.</title>
<author><name sortKey="Seyama, T" sort="Seyama, T" uniqKey="Seyama T" first="T" last="Seyama">T. Seyama</name>
<affiliation><nlm:affiliation>Department of Radiobiology, Radiation Effects Research Foundation, Hiroshima, Japan.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Ito, T" sort="Ito, T" uniqKey="Ito T" first="T" last="Ito">T. Ito</name>
</author>
<author><name sortKey="Hayashi, T" sort="Hayashi, T" uniqKey="Hayashi T" first="T" last="Hayashi">T. Hayashi</name>
</author>
<author><name sortKey="Mizuno, T" sort="Mizuno, T" uniqKey="Mizuno T" first="T" last="Mizuno">T. Mizuno</name>
</author>
<author><name sortKey="Nakamura, N" sort="Nakamura, N" uniqKey="Nakamura N" first="N" last="Nakamura">N. Nakamura</name>
</author>
<author><name sortKey="Akiyama, M" sort="Akiyama, M" uniqKey="Akiyama M" first="M" last="Akiyama">M. Akiyama</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="1992">1992</date>
<idno type="RBID">pubmed:1598207</idno>
<idno type="pmid">1598207</idno>
<idno type="doi">10.1093/nar/20.10.2493</idno>
<idno type="wicri:Area/PubMed/Corpus">002958</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002958</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">A novel blocker-PCR method for detection of rare mutant alleles in the presence of an excess amount of normal DNA.</title>
<author><name sortKey="Seyama, T" sort="Seyama, T" uniqKey="Seyama T" first="T" last="Seyama">T. Seyama</name>
<affiliation><nlm:affiliation>Department of Radiobiology, Radiation Effects Research Foundation, Hiroshima, Japan.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Ito, T" sort="Ito, T" uniqKey="Ito T" first="T" last="Ito">T. Ito</name>
</author>
<author><name sortKey="Hayashi, T" sort="Hayashi, T" uniqKey="Hayashi T" first="T" last="Hayashi">T. Hayashi</name>
</author>
<author><name sortKey="Mizuno, T" sort="Mizuno, T" uniqKey="Mizuno T" first="T" last="Mizuno">T. Mizuno</name>
</author>
<author><name sortKey="Nakamura, N" sort="Nakamura, N" uniqKey="Nakamura N" first="N" last="Nakamura">N. Nakamura</name>
</author>
<author><name sortKey="Akiyama, M" sort="Akiyama, M" uniqKey="Akiyama M" first="M" last="Akiyama">M. Akiyama</name>
</author>
</analytic>
<series><title level="j">Nucleic acids research</title>
<idno type="ISSN">0305-1048</idno>
<imprint><date when="1992" type="published">1992</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alleles</term>
<term>Base Sequence</term>
<term>DNA, Single-Stranded (genetics)</term>
<term>Genes, ras (genetics)</term>
<term>Humans</term>
<term>Molecular Sequence Data</term>
<term>Mutation (genetics)</term>
<term>Nucleic Acid Conformation</term>
<term>Oligodeoxyribonucleotides (genetics)</term>
<term>Polymerase Chain Reaction (methods)</term>
<term>Polymorphism, Genetic (genetics)</term>
<term>Sensitivity and Specificity</term>
<term>Temperature</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Single-Stranded</term>
<term>Oligodeoxyribonucleotides</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genes, ras</term>
<term>Mutation</term>
<term>Polymorphism, Genetic</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Alleles</term>
<term>Base Sequence</term>
<term>Humans</term>
<term>Molecular Sequence Data</term>
<term>Nucleic Acid Conformation</term>
<term>Sensitivity and Specificity</term>
<term>Temperature</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A novel polymerase chain reaction method was developed to preferentially amplify a segment of DNA containing a base substitution mutation. This technique uses a pair of dideoxynucleotide-labeled oligonucleotides (18 mers) of normal sequences as blockers located between the two primers. By virtue of a subtle difference in the melting temperature between the blocker-normal DNA and blocker-mutant DNA hybrids, the method allows preferential amplification of the mutant DNA. We used the human N-ras gene as a model. Two different types of N-ras mutations could be effectively amplified when they were present with an excess amount of normal DNA at a ratio of 1:10(3). Furthermore, the sensitivity was increased 10-fold by using single strand conformation polymorphism analysis for the amplified products, and mutant DNA was detected in the presence of a 10(4) times excess amount of normal DNA.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">1598207</PMID>
<DateCompleted><Year>1992</Year>
<Month>07</Month>
<Day>09</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>05</Month>
<Day>01</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0305-1048</ISSN>
<JournalIssue CitedMedium="Print"><Volume>20</Volume>
<Issue>10</Issue>
<PubDate><Year>1992</Year>
<Month>May</Month>
<Day>25</Day>
</PubDate>
</JournalIssue>
<Title>Nucleic acids research</Title>
<ISOAbbreviation>Nucleic Acids Res.</ISOAbbreviation>
</Journal>
<ArticleTitle>A novel blocker-PCR method for detection of rare mutant alleles in the presence of an excess amount of normal DNA.</ArticleTitle>
<Pagination><MedlinePgn>2493-6</MedlinePgn>
</Pagination>
<Abstract><AbstractText>A novel polymerase chain reaction method was developed to preferentially amplify a segment of DNA containing a base substitution mutation. This technique uses a pair of dideoxynucleotide-labeled oligonucleotides (18 mers) of normal sequences as blockers located between the two primers. By virtue of a subtle difference in the melting temperature between the blocker-normal DNA and blocker-mutant DNA hybrids, the method allows preferential amplification of the mutant DNA. We used the human N-ras gene as a model. Two different types of N-ras mutations could be effectively amplified when they were present with an excess amount of normal DNA at a ratio of 1:10(3). Furthermore, the sensitivity was increased 10-fold by using single strand conformation polymorphism analysis for the amplified products, and mutant DNA was detected in the presence of a 10(4) times excess amount of normal DNA.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Seyama</LastName>
<ForeName>T</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Department of Radiobiology, Radiation Effects Research Foundation, Hiroshima, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ito</LastName>
<ForeName>T</ForeName>
<Initials>T</Initials>
</Author>
<Author ValidYN="Y"><LastName>Hayashi</LastName>
<ForeName>T</ForeName>
<Initials>T</Initials>
</Author>
<Author ValidYN="Y"><LastName>Mizuno</LastName>
<ForeName>T</ForeName>
<Initials>T</Initials>
</Author>
<Author ValidYN="Y"><LastName>Nakamura</LastName>
<ForeName>N</ForeName>
<Initials>N</Initials>
</Author>
<Author ValidYN="Y"><LastName>Akiyama</LastName>
<ForeName>M</ForeName>
<Initials>M</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Nucleic Acids Res</MedlineTA>
<NlmUniqueID>0411011</NlmUniqueID>
<ISSNLinking>0305-1048</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D004277">DNA, Single-Stranded</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D009838">Oligodeoxyribonucleotides</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<GeneSymbolList><GeneSymbol>ras</GeneSymbol>
</GeneSymbolList>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000483" MajorTopicYN="N">Alleles</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004277" MajorTopicYN="N">DNA, Single-Stranded</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011905" MajorTopicYN="N">Genes, ras</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009690" MajorTopicYN="N">Nucleic Acid Conformation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009838" MajorTopicYN="N">Oligodeoxyribonucleotides</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011110" MajorTopicYN="N">Polymorphism, Genetic</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1992</Year>
<Month>5</Month>
<Day>25</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>1992</Year>
<Month>5</Month>
<Day>25</Day>
<Hour>0</Hour>
<Minute>1</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>1992</Year>
<Month>5</Month>
<Day>25</Day>
<Hour>0</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">1598207</ArticleId>
<ArticleId IdType="pmc">PMC312383</ArticleId>
<ArticleId IdType="doi">10.1093/nar/20.10.2493</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Nature. 1989 Dec 7;342(6250):705-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2531845</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1989 Oct 25;17(20):8093-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2573037</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 1989 Apr 28;160(2):441-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2655589</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1989 Dec 15;246(4936):1406-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2574499</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 1983 Sep;34(2):581-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6616621</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cancer Res. 1989 Sep 1;49(17):4682-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2547513</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1990 Jun 1;248(4959):1101-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2188364</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1990 Feb 25;18(4):999-1005</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2179874</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Enzymol. 1983;100:96-116</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6312266</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1989 Apr;86(8):2766-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2565038</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Corpus

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002958 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 002958 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    PubMed
   |étape=   Corpus
   |type=    RBID
   |clé=     pubmed:1598207
   |texte=   A novel blocker-PCR method for detection of rare mutant alleles in the presence of an excess amount of normal DNA.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i   -Sk "pubmed:1598207" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd   \
       | NlmPubMed2Wicri -a MersV1

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration MERS

A novel blocker-PCR method for detection of rare mutant alleles in the presence of an excess amount of normal DNA.

A novel blocker-PCR method for detection of rare mutant alleles in the presence of an excess amount of normal DNA.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki