Identification of aptamers against the DNA template for in vitro transcription of the HIV-1 TAR element.
Identifieur interne : 002713 ( PubMed/Corpus ); précédent : 002712; suivant : 002714Identification of aptamers against the DNA template for in vitro transcription of the HIV-1 TAR element.
Auteurs : C. Boiziau ; E. Dausse ; R. Mishra ; F. Ducongé ; J J ToulméSource :
- Antisense & nucleic acid drug development [ 1087-2906 ] ; 1997.
English descriptors
- KwdEn :
- Base Composition, Base Sequence, Consensus Sequence, DNA, Viral (chemistry), DNA, Viral (metabolism), HIV Long Terminal Repeat, HIV-1 (genetics), HIV-1 (metabolism), Humans, Molecular Sequence Data, Oligonucleotides, Antisense (pharmacology), RNA, Viral (chemistry), RNA, Viral (metabolism), Sequence Alignment, Templates, Genetic, Transcription, Genetic (drug effects).
- MESH :
- chemical , chemistry : DNA, Viral, RNA, Viral.
- chemical , metabolism : DNA, Viral, RNA, Viral.
- drug effects : Transcription, Genetic.
- genetics : HIV-1.
- metabolism : HIV-1.
- chemical , pharmacology : Oligonucleotides, Antisense.
- Base Composition, Base Sequence, Consensus Sequence, HIV Long Terminal Repeat, Humans, Molecular Sequence Data, Sequence Alignment, Templates, Genetic.
Abstract
We have extracted from a random population of about 10(9) oligodeoxynucleotides a series of 21-mers that are able to bind to a folded DNA 76-mer used as a template for in vitro transcription of the TAR element of the retrovirus HIV-1, by the T7 RNA polymerase. Five aptastrucs, that is, aptamers able to bind to the structure, out of 15 analyzed sequences, share the consensus motif 5'-PyGGG(TG)PyC, complementary in part to a weak double-stranded region of the target. (The parentheses indicate that either T or G is missing in one of these aptastrucs.) A dissociation constant of about 3 microM was evaluated by electrophoretic mobility shift assay for the winner sequence. Interactions between the aptastruc and the target sequences involve more than Watson-Crick base pairing of the consensus octamer. The binding is chemistry dependent. Phosphorothioate oligodeoxyribonucleotides and 2'-O-methyl oligoribonucleotides derived from the selected aptastrucs exhibit a weak if any affinity for the target.
DOI: 10.1089/oli.1.1997.7.369
PubMed: 9303189
Links to Exploration step
pubmed:9303189Le document en format XML
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Ducongé</name></author><author><name sortKey="Toulme, J J" sort="Toulme, J J" uniqKey="Toulme J" first="J J" last="Toulmé">J J Toulmé</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1997">1997</date><idno type="RBID">pubmed:9303189</idno><idno type="pmid">9303189</idno><idno type="doi">10.1089/oli.1.1997.7.369</idno><idno type="wicri:Area/PubMed/Corpus">002713</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002713</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification of aptamers against the DNA template for in vitro transcription of the HIV-1 TAR element.</title><author><name sortKey="Boiziau, C" sort="Boiziau, C" uniqKey="Boiziau C" first="C" last="Boiziau">C. Boiziau</name><affiliation><nlm:affiliation>INSERM U 386, IFR Pathologies Infectieuses, Université Victor Segalen, Bordeaux, France.</nlm:affiliation></affiliation></author><author><name sortKey="Dausse, E" sort="Dausse, E" uniqKey="Dausse E" first="E" last="Dausse">E. Dausse</name></author><author><name sortKey="Mishra, R" sort="Mishra, R" uniqKey="Mishra R" first="R" last="Mishra">R. Mishra</name></author><author><name sortKey="Duconge, F" sort="Duconge, F" uniqKey="Duconge F" first="F" last="Ducongé">F. Ducongé</name></author><author><name sortKey="Toulme, J J" sort="Toulme, J J" uniqKey="Toulme J" first="J J" last="Toulmé">J J Toulmé</name></author></analytic><series><title level="j">Antisense & nucleic acid drug development</title><idno type="ISSN">1087-2906</idno><imprint><date when="1997" type="published">1997</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Composition</term><term>Base Sequence</term><term>Consensus Sequence</term><term>DNA, Viral (chemistry)</term><term>DNA, Viral (metabolism)</term><term>HIV Long Terminal Repeat</term><term>HIV-1 (genetics)</term><term>HIV-1 (metabolism)</term><term>Humans</term><term>Molecular Sequence Data</term><term>Oligonucleotides, Antisense (pharmacology)</term><term>RNA, Viral (chemistry)</term><term>RNA, Viral (metabolism)</term><term>Sequence Alignment</term><term>Templates, Genetic</term><term>Transcription, Genetic (drug effects)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Viral</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA, Viral</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Transcription, Genetic</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>HIV-1</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>HIV-1</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Oligonucleotides, Antisense</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Composition</term><term>Base Sequence</term><term>Consensus Sequence</term><term>HIV Long Terminal Repeat</term><term>Humans</term><term>Molecular Sequence Data</term><term>Sequence Alignment</term><term>Templates, Genetic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have extracted from a random population of about 10(9) oligodeoxynucleotides a series of 21-mers that are able to bind to a folded DNA 76-mer used as a template for in vitro transcription of the TAR element of the retrovirus HIV-1, by the T7 RNA polymerase. Five aptastrucs, that is, aptamers able to bind to the structure, out of 15 analyzed sequences, share the consensus motif 5'-PyGGG(TG)PyC, complementary in part to a weak double-stranded region of the target. (The parentheses indicate that either T or G is missing in one of these aptastrucs.) A dissociation constant of about 3 microM was evaluated by electrophoretic mobility shift assay for the winner sequence. Interactions between the aptastruc and the target sequences involve more than Watson-Crick base pairing of the consensus octamer. The binding is chemistry dependent. Phosphorothioate oligodeoxyribonucleotides and 2'-O-methyl oligoribonucleotides derived from the selected aptastrucs exhibit a weak if any affinity for the target.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">9303189</PMID><DateCompleted><Year>1998</Year><Month>02</Month><Day>05</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1087-2906</ISSN><JournalIssue CitedMedium="Print"><Volume>7</Volume><Issue>4</Issue><PubDate><Year>1997</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Antisense & nucleic acid drug development</Title><ISOAbbreviation>Antisense Nucleic Acid Drug Dev.</ISOAbbreviation></Journal><ArticleTitle>Identification of aptamers against the DNA template for in vitro transcription of the HIV-1 TAR element.</ArticleTitle><Pagination><MedlinePgn>369-80</MedlinePgn></Pagination><Abstract><AbstractText>We have extracted from a random population of about 10(9) oligodeoxynucleotides a series of 21-mers that are able to bind to a folded DNA 76-mer used as a template for in vitro transcription of the TAR element of the retrovirus HIV-1, by the T7 RNA polymerase. Five aptastrucs, that is, aptamers able to bind to the structure, out of 15 analyzed sequences, share the consensus motif 5'-PyGGG(TG)PyC, complementary in part to a weak double-stranded region of the target. (The parentheses indicate that either T or G is missing in one of these aptastrucs.) A dissociation constant of about 3 microM was evaluated by electrophoretic mobility shift assay for the winner sequence. Interactions between the aptastruc and the target sequences involve more than Watson-Crick base pairing of the consensus octamer. The binding is chemistry dependent. Phosphorothioate oligodeoxyribonucleotides and 2'-O-methyl oligoribonucleotides derived from the selected aptastrucs exhibit a weak if any affinity for the target.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Boiziau</LastName><ForeName>C</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>INSERM U 386, IFR Pathologies Infectieuses, Université Victor Segalen, Bordeaux, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dausse</LastName><ForeName>E</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Mishra</LastName><ForeName>R</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Ducongé</LastName><ForeName>F</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Toulmé</LastName><ForeName>J J</ForeName><Initials>JJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Antisense Nucleic Acid Drug Dev</MedlineTA><NlmUniqueID>9606142</NlmUniqueID><ISSNLinking>1087-2906</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004279">DNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016376">Oligonucleotides, Antisense</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CitationSubset>X</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001482" MajorTopicYN="N">Base Composition</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016384" MajorTopicYN="N">Consensus Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004279" MajorTopicYN="N">DNA, Viral</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016325" MajorTopicYN="Y">HIV Long Terminal Repeat</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015497" MajorTopicYN="N">HIV-1</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016376" MajorTopicYN="N">Oligonucleotides, Antisense</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013698" MajorTopicYN="N">Templates, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1997</Year><Month>8</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1997</Year><Month>9</Month><Day>26</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1997</Year><Month>8</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">9303189</ArticleId><ArticleId IdType="doi">10.1089/oli.1.1997.7.369</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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