Genetic and alkaloid analysis of Papaver species and their F1 hybrid by RAPD, HPLC and ELISA.
Identifieur interne : 002697 ( PubMed/Corpus ); précédent : 002696; suivant : 002698Genetic and alkaloid analysis of Papaver species and their F1 hybrid by RAPD, HPLC and ELISA.
Auteurs : Y. Shoyama ; F. Kawachi ; H. Tanaka ; R. Nakai ; T. Shibata ; K. NishiSource :
- Forensic science international [ 0379-0738 ] ; 1998.
English descriptors
- KwdEn :
- Alkaloids (analysis), Biomarkers (analysis), Chromatography, High Pressure Liquid, Chromosomes (genetics), DNA Primers (chemistry), DNA, Plant (analysis), Enzyme-Linked Immunosorbent Assay, Narcotics (chemistry), Papaver (chemistry), Papaver (genetics), Plants, Medicinal, Random Amplified Polymorphic DNA Technique, Species Specificity, Thebaine (chemistry).
- MESH :
- chemical , analysis : Alkaloids, Biomarkers, DNA, Plant.
- chemical , chemistry : DNA Primers, Narcotics, Thebaine.
- chemistry : Papaver.
- genetics : Chromosomes, Papaver.
- Chromatography, High Pressure Liquid, Enzyme-Linked Immunosorbent Assay, Plants, Medicinal, Random Amplified Polymorphic DNA Technique, Species Specificity.
Abstract
Total DNA was extracted from the leaves of a F1 hybrid and its parents, Papaver bracteatum Lindle and P. pseudo-orientale Medw. Analysis of random-amplified polymorphic DNA (RAPD) using ten arbitrary oligonucleotide 10-mers, showed that F1 hybrid was confirmed to be genetically intermediate of both parental plants compared with the genetic distance between F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between a F1 hybrid, P. bracteatum and P. pseudo-orientale clearly showed that part of the bands of both parents were induced into a F1 hybrid. The content of thebaine was determined by HPLC and ELISA used anti-thebaine monoclonal antibody.
DOI: 10.1016/s0379-0738(97)00196-5
PubMed: 9530831
Links to Exploration step
pubmed:9530831Le document en format XML
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Nishi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1998">1998</date><idno type="RBID">pubmed:9530831</idno><idno type="pmid">9530831</idno><idno type="doi">10.1016/s0379-0738(97)00196-5</idno><idno type="wicri:Area/PubMed/Corpus">002697</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002697</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Genetic and alkaloid analysis of Papaver species and their F1 hybrid by RAPD, HPLC and ELISA.</title><author><name sortKey="Shoyama, Y" sort="Shoyama, Y" uniqKey="Shoyama Y" first="Y" last="Shoyama">Y. Shoyama</name><affiliation><nlm:affiliation>Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Kawachi, F" sort="Kawachi, F" uniqKey="Kawachi F" first="F" last="Kawachi">F. Kawachi</name></author><author><name sortKey="Tanaka, H" sort="Tanaka, H" uniqKey="Tanaka H" first="H" last="Tanaka">H. Tanaka</name></author><author><name sortKey="Nakai, R" sort="Nakai, R" uniqKey="Nakai R" first="R" last="Nakai">R. Nakai</name></author><author><name sortKey="Shibata, T" sort="Shibata, T" uniqKey="Shibata T" first="T" last="Shibata">T. Shibata</name></author><author><name sortKey="Nishi, K" sort="Nishi, K" uniqKey="Nishi K" first="K" last="Nishi">K. Nishi</name></author></analytic><series><title level="j">Forensic science international</title><idno type="ISSN">0379-0738</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alkaloids (analysis)</term><term>Biomarkers (analysis)</term><term>Chromatography, High Pressure Liquid</term><term>Chromosomes (genetics)</term><term>DNA Primers (chemistry)</term><term>DNA, Plant (analysis)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Narcotics (chemistry)</term><term>Papaver (chemistry)</term><term>Papaver (genetics)</term><term>Plants, Medicinal</term><term>Random Amplified Polymorphic DNA Technique</term><term>Species Specificity</term><term>Thebaine (chemistry)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Alkaloids</term><term>Biomarkers</term><term>DNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA Primers</term><term>Narcotics</term><term>Thebaine</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Papaver</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Chromosomes</term><term>Papaver</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Plants, Medicinal</term><term>Random Amplified Polymorphic DNA Technique</term><term>Species Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Total DNA was extracted from the leaves of a F1 hybrid and its parents, Papaver bracteatum Lindle and P. pseudo-orientale Medw. Analysis of random-amplified polymorphic DNA (RAPD) using ten arbitrary oligonucleotide 10-mers, showed that F1 hybrid was confirmed to be genetically intermediate of both parental plants compared with the genetic distance between F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between a F1 hybrid, P. bracteatum and P. pseudo-orientale clearly showed that part of the bands of both parents were induced into a F1 hybrid. The content of thebaine was determined by HPLC and ELISA used anti-thebaine monoclonal antibody.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">9530831</PMID><DateCompleted><Year>1998</Year><Month>04</Month><Day>24</Day></DateCompleted><DateRevised><Year>2019</Year><Month>08</Month><Day>31</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0379-0738</ISSN><JournalIssue CitedMedium="Print"><Volume>91</Volume><Issue>3</Issue><PubDate><Year>1998</Year><Month>Feb</Month><Day>16</Day></PubDate></JournalIssue><Title>Forensic science international</Title><ISOAbbreviation>Forensic Sci. Int.</ISOAbbreviation></Journal><ArticleTitle>Genetic and alkaloid analysis of Papaver species and their F1 hybrid by RAPD, HPLC and ELISA.</ArticleTitle><Pagination><MedlinePgn>207-17</MedlinePgn></Pagination><Abstract><AbstractText>Total DNA was extracted from the leaves of a F1 hybrid and its parents, Papaver bracteatum Lindle and P. pseudo-orientale Medw. Analysis of random-amplified polymorphic DNA (RAPD) using ten arbitrary oligonucleotide 10-mers, showed that F1 hybrid was confirmed to be genetically intermediate of both parental plants compared with the genetic distance between F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between a F1 hybrid, P. bracteatum and P. pseudo-orientale clearly showed that part of the bands of both parents were induced into a F1 hybrid. The content of thebaine was determined by HPLC and ELISA used anti-thebaine monoclonal antibody.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shoyama</LastName><ForeName>Y</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kawachi</LastName><ForeName>F</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Tanaka</LastName><ForeName>H</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Nakai</LastName><ForeName>R</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Shibata</LastName><ForeName>T</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Nishi</LastName><ForeName>K</ForeName><Initials>K</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Ireland</Country><MedlineTA>Forensic Sci Int</MedlineTA><NlmUniqueID>7902034</NlmUniqueID><ISSNLinking>0379-0738</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000470">Alkaloids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015415">Biomarkers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018744">DNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009294">Narcotics</NameOfSubstance></Chemical><Chemical><RegistryNumber>2P9MKG8GX7</RegistryNumber><NameOfSubstance UI="D013797">Thebaine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000470" MajorTopicYN="N">Alkaloids</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015415" MajorTopicYN="N">Biomarkers</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002851" MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002875" MajorTopicYN="N">Chromosomes</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018744" MajorTopicYN="N">DNA, Plant</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009294" MajorTopicYN="N">Narcotics</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010207" MajorTopicYN="N">Papaver</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010946" MajorTopicYN="Y">Plants, Medicinal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019105" MajorTopicYN="N">Random Amplified Polymorphic DNA Technique</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013045" MajorTopicYN="N">Species Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013797" MajorTopicYN="N">Thebaine</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1998</Year><Month>4</Month><Day>8</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1998</Year><Month>4</Month><Day>8</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1998</Year><Month>4</Month><Day>8</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">9530831</ArticleId><ArticleId IdType="pii">S0379-0738(97)00196-5</ArticleId><ArticleId IdType="doi">10.1016/s0379-0738(97)00196-5</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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