Effect of single benzo[a]pyrene diol epoxide-deoxyguanosine adducts on the action of DNA polymerases in vitro.
Identifieur interne : 002679 ( PubMed/Corpus ); précédent : 002678; suivant : 002680Effect of single benzo[a]pyrene diol epoxide-deoxyguanosine adducts on the action of DNA polymerases in vitro.
Auteurs : L J Lipinski ; H L Ross ; B. Zajc ; J M Sayer ; D M Jerina ; A. DippleSource :
- International journal of oncology [ 1019-6439 ] ; 1998.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide, DNA Adducts, DNA Polymerase I, Deoxyguanosine, Oligonucleotides.
- DNA Replication, Mutation, Templates, Genetic.
Abstract
Neither Sequenase 2.0 nor Klenow fragment were able to extend 12-mer primers using the eight templates (16-mers) derived by placing each of the four isomeric benzo[a]pyrene diol epoxide-deoxyguanosine adducts at the 13th nucleotide from the 3'-end of two different sequence contexts. Using an 11-mer primer to get a running start did not overcome the adduct induced block of primer extension except for the Klenow fragment and one of the two sequence contexts, indicating primer extension is dependent on both the polymerase and sequence context. In this case, purine nucleoside triphosphates (dATP>dGTP) were incorporated opposite each of the four adducts.
DOI: 10.3892/ijo.13.2.269
PubMed: 9664121
Links to Exploration step
pubmed:9664121Le document en format XML
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<author><name sortKey="Lipinski, L J" sort="Lipinski, L J" uniqKey="Lipinski L" first="L J" last="Lipinski">L J Lipinski</name>
<affiliation><nlm:affiliation>Laboratory of Molecular Genetics, National Institute on Aging, NIH, Baltimore, MD 21224, USA.</nlm:affiliation>
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<author><name sortKey="Ross, H L" sort="Ross, H L" uniqKey="Ross H" first="H L" last="Ross">H L Ross</name>
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<author><name sortKey="Zajc, B" sort="Zajc, B" uniqKey="Zajc B" first="B" last="Zajc">B. Zajc</name>
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<author><name sortKey="Sayer, J M" sort="Sayer, J M" uniqKey="Sayer J" first="J M" last="Sayer">J M Sayer</name>
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<author><name sortKey="Jerina, D M" sort="Jerina, D M" uniqKey="Jerina D" first="D M" last="Jerina">D M Jerina</name>
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<author><name sortKey="Dipple, A" sort="Dipple, A" uniqKey="Dipple A" first="A" last="Dipple">A. Dipple</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Effect of single benzo[a]pyrene diol epoxide-deoxyguanosine adducts on the action of DNA polymerases in vitro.</title>
<author><name sortKey="Lipinski, L J" sort="Lipinski, L J" uniqKey="Lipinski L" first="L J" last="Lipinski">L J Lipinski</name>
<affiliation><nlm:affiliation>Laboratory of Molecular Genetics, National Institute on Aging, NIH, Baltimore, MD 21224, USA.</nlm:affiliation>
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<author><name sortKey="Ross, H L" sort="Ross, H L" uniqKey="Ross H" first="H L" last="Ross">H L Ross</name>
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<author><name sortKey="Zajc, B" sort="Zajc, B" uniqKey="Zajc B" first="B" last="Zajc">B. Zajc</name>
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<author><name sortKey="Sayer, J M" sort="Sayer, J M" uniqKey="Sayer J" first="J M" last="Sayer">J M Sayer</name>
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<author><name sortKey="Jerina, D M" sort="Jerina, D M" uniqKey="Jerina D" first="D M" last="Jerina">D M Jerina</name>
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<author><name sortKey="Dipple, A" sort="Dipple, A" uniqKey="Dipple A" first="A" last="Dipple">A. Dipple</name>
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<series><title level="j">International journal of oncology</title>
<idno type="ISSN">1019-6439</idno>
<imprint><date when="1998" type="published">1998</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide (metabolism)</term>
<term>DNA Adducts (metabolism)</term>
<term>DNA Polymerase I (metabolism)</term>
<term>DNA Replication</term>
<term>Deoxyguanosine (metabolism)</term>
<term>Mutation</term>
<term>Oligonucleotides (metabolism)</term>
<term>Templates, Genetic</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide</term>
<term>DNA Adducts</term>
<term>DNA Polymerase I</term>
<term>Deoxyguanosine</term>
<term>Oligonucleotides</term>
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<keywords scheme="MESH" xml:lang="en"><term>DNA Replication</term>
<term>Mutation</term>
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<front><div type="abstract" xml:lang="en">Neither Sequenase 2.0 nor Klenow fragment were able to extend 12-mer primers using the eight templates (16-mers) derived by placing each of the four isomeric benzo[a]pyrene diol epoxide-deoxyguanosine adducts at the 13th nucleotide from the 3'-end of two different sequence contexts. Using an 11-mer primer to get a running start did not overcome the adduct induced block of primer extension except for the Klenow fragment and one of the two sequence contexts, indicating primer extension is dependent on both the polymerase and sequence context. In this case, purine nucleoside triphosphates (dATP>dGTP) were incorporated opposite each of the four adducts.</div>
</front>
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<Issue>2</Issue>
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<Month>Aug</Month>
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<Title>International journal of oncology</Title>
<ISOAbbreviation>Int. J. Oncol.</ISOAbbreviation>
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<ArticleTitle>Effect of single benzo[a]pyrene diol epoxide-deoxyguanosine adducts on the action of DNA polymerases in vitro.</ArticleTitle>
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<Abstract><AbstractText>Neither Sequenase 2.0 nor Klenow fragment were able to extend 12-mer primers using the eight templates (16-mers) derived by placing each of the four isomeric benzo[a]pyrene diol epoxide-deoxyguanosine adducts at the 13th nucleotide from the 3'-end of two different sequence contexts. Using an 11-mer primer to get a running start did not overcome the adduct induced block of primer extension except for the Klenow fragment and one of the two sequence contexts, indicating primer extension is dependent on both the polymerase and sequence context. In this case, purine nucleoside triphosphates (dATP>dGTP) were incorporated opposite each of the four adducts.</AbstractText>
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<MeshHeading><DescriptorName UI="D004261" MajorTopicYN="N">DNA Replication</DescriptorName>
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<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
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<MeshHeading><DescriptorName UI="D013698" MajorTopicYN="N">Templates, Genetic</DescriptorName>
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