Selective binding of nucleotide probes by eosinophilic cationic protein during in situ hybridisation.
Identifieur interne : 002477 ( PubMed/Corpus ); précédent : 002476; suivant : 002478Selective binding of nucleotide probes by eosinophilic cationic protein during in situ hybridisation.
Auteurs : Richard D. Ellis ; Paul Ashwood ; Jonathan J. Powell ; Paul D. Taylor ; Richard Poulsom ; Richard P H. Thompson ; Neville A. PunchardSource :
- The Histochemical journal [ 0018-2214 ]
English descriptors
- KwdEn :
- Antisense Elements (Genetics), Autoradiography, Blood Proteins (metabolism), Chromatography, High Pressure Liquid, Colon (metabolism), Colon (pathology), Colonic Polyps (metabolism), Colonic Polyps (pathology), Coloring Agents, Crohn Disease (metabolism), Crohn Disease (pathology), DNA Probes, Dithiothreitol, Eosinophil Granule Proteins, Eosinophils (metabolism), Exons, Humans, In Situ Hybridization, NF-kappa B (drug effects), NF-kappa B (metabolism), Nucleotides (metabolism), Paraffin Embedding, RNA, Messenger (biosynthesis), Ribonucleases, Sulfhydryl Reagents, Tumor Necrosis Factor-alpha (metabolism).
- MESH :
- chemical , biosynthesis : RNA, Messenger.
- chemical , drug effects : NF-kappa B.
- chemical , metabolism : Blood Proteins, NF-kappa B, Nucleotides, Tumor Necrosis Factor-alpha.
- chemical : Antisense Elements (Genetics), Coloring Agents, DNA Probes, Dithiothreitol, Eosinophil Granule Proteins, Ribonucleases, Sulfhydryl Reagents.
- metabolism : Colon, Colonic Polyps, Crohn Disease, Eosinophils.
- pathology : Colon, Colonic Polyps, Crohn Disease.
- Autoradiography, Chromatography, High Pressure Liquid, Exons, Humans, In Situ Hybridization, Paraffin Embedding.
Abstract
During in situ hybridisation on frozen and paraffin-embedded sections of bowel for IkappaB alpha, oligodeoxyribonucleotide probes were found to bind more avidly to eosinophils than target mRNA. This binding could not be obviated using strategies previously employed to block either binding of long DNA probes (200-mers) to eosinophils in bone marrow smears, or of riboprobes to eosinophils in sections of bowel, without removing specific hybridisation of probes. That this binding could arise through interaction of anionic oligodeoxyribonucleotides with eosinophil cationic protein, which has an unusually high pI, and is abundant in cytoplasmic granules of eosinophils, was demonstrated in vitro using real-time biomolecular interaction analysis with a BiacoreX instrument. Finally, a relationship between probe hydrophobicity, measured by reverse phase ion-pair high performance liquid chromatography, and in situ binding of individual probes to eosinophils was demonstrated. Effective tissue penetration by hydrophobic probes and subsequent strong probe-eosinophilic cationic protein interactions therefore may confound the interpretation of in situ hybridisation performed with oligonucleotide probes in eosinophil-containing tissues, such as bowel and nasal polyps.
DOI: 10.1023/a:1020942531028
PubMed: 12495221
Links to Exploration step
pubmed:12495221Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Selective binding of nucleotide probes by eosinophilic cationic protein during in situ hybridisation.</title>
<author><name sortKey="Ellis, Richard D" sort="Ellis, Richard D" uniqKey="Ellis R" first="Richard D" last="Ellis">Richard D. Ellis</name>
<affiliation><nlm:affiliation>Gastrointestinal Laboratory, The Rayne Institute, St. Thomas' Hospital, London, SE1 7EH, UK.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Ashwood, Paul" sort="Ashwood, Paul" uniqKey="Ashwood P" first="Paul" last="Ashwood">Paul Ashwood</name>
</author>
<author><name sortKey="Powell, Jonathan J" sort="Powell, Jonathan J" uniqKey="Powell J" first="Jonathan J" last="Powell">Jonathan J. Powell</name>
</author>
<author><name sortKey="Taylor, Paul D" sort="Taylor, Paul D" uniqKey="Taylor P" first="Paul D" last="Taylor">Paul D. Taylor</name>
</author>
<author><name sortKey="Poulsom, Richard" sort="Poulsom, Richard" uniqKey="Poulsom R" first="Richard" last="Poulsom">Richard Poulsom</name>
</author>
<author><name sortKey="Thompson, Richard P H" sort="Thompson, Richard P H" uniqKey="Thompson R" first="Richard P H" last="Thompson">Richard P H. Thompson</name>
</author>
<author><name sortKey="Punchard, Neville A" sort="Punchard, Neville A" uniqKey="Punchard N" first="Neville A" last="Punchard">Neville A. Punchard</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="????"><PubDate><MedlineDate>2002 Mar-Apr</MedlineDate>
</PubDate>
</date>
<idno type="RBID">pubmed:12495221</idno>
<idno type="pmid">12495221</idno>
<idno type="doi">10.1023/a:1020942531028</idno>
<idno type="wicri:Area/PubMed/Corpus">002477</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002477</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Selective binding of nucleotide probes by eosinophilic cationic protein during in situ hybridisation.</title>
<author><name sortKey="Ellis, Richard D" sort="Ellis, Richard D" uniqKey="Ellis R" first="Richard D" last="Ellis">Richard D. Ellis</name>
<affiliation><nlm:affiliation>Gastrointestinal Laboratory, The Rayne Institute, St. Thomas' Hospital, London, SE1 7EH, UK.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Ashwood, Paul" sort="Ashwood, Paul" uniqKey="Ashwood P" first="Paul" last="Ashwood">Paul Ashwood</name>
</author>
<author><name sortKey="Powell, Jonathan J" sort="Powell, Jonathan J" uniqKey="Powell J" first="Jonathan J" last="Powell">Jonathan J. Powell</name>
</author>
<author><name sortKey="Taylor, Paul D" sort="Taylor, Paul D" uniqKey="Taylor P" first="Paul D" last="Taylor">Paul D. Taylor</name>
</author>
<author><name sortKey="Poulsom, Richard" sort="Poulsom, Richard" uniqKey="Poulsom R" first="Richard" last="Poulsom">Richard Poulsom</name>
</author>
<author><name sortKey="Thompson, Richard P H" sort="Thompson, Richard P H" uniqKey="Thompson R" first="Richard P H" last="Thompson">Richard P H. Thompson</name>
</author>
<author><name sortKey="Punchard, Neville A" sort="Punchard, Neville A" uniqKey="Punchard N" first="Neville A" last="Punchard">Neville A. Punchard</name>
</author>
</analytic>
<series><title level="j">The Histochemical journal</title>
<idno type="ISSN">0018-2214</idno>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antisense Elements (Genetics)</term>
<term>Autoradiography</term>
<term>Blood Proteins (metabolism)</term>
<term>Chromatography, High Pressure Liquid</term>
<term>Colon (metabolism)</term>
<term>Colon (pathology)</term>
<term>Colonic Polyps (metabolism)</term>
<term>Colonic Polyps (pathology)</term>
<term>Coloring Agents</term>
<term>Crohn Disease (metabolism)</term>
<term>Crohn Disease (pathology)</term>
<term>DNA Probes</term>
<term>Dithiothreitol</term>
<term>Eosinophil Granule Proteins</term>
<term>Eosinophils (metabolism)</term>
<term>Exons</term>
<term>Humans</term>
<term>In Situ Hybridization</term>
<term>NF-kappa B (drug effects)</term>
<term>NF-kappa B (metabolism)</term>
<term>Nucleotides (metabolism)</term>
<term>Paraffin Embedding</term>
<term>RNA, Messenger (biosynthesis)</term>
<term>Ribonucleases</term>
<term>Sulfhydryl Reagents</term>
<term>Tumor Necrosis Factor-alpha (metabolism)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>RNA, Messenger</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="drug effects" xml:lang="en"><term>NF-kappa B</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Blood Proteins</term>
<term>NF-kappa B</term>
<term>Nucleotides</term>
<term>Tumor Necrosis Factor-alpha</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antisense Elements (Genetics)</term>
<term>Coloring Agents</term>
<term>DNA Probes</term>
<term>Dithiothreitol</term>
<term>Eosinophil Granule Proteins</term>
<term>Ribonucleases</term>
<term>Sulfhydryl Reagents</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Colon</term>
<term>Colonic Polyps</term>
<term>Crohn Disease</term>
<term>Eosinophils</term>
</keywords>
<keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Colon</term>
<term>Colonic Polyps</term>
<term>Crohn Disease</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Autoradiography</term>
<term>Chromatography, High Pressure Liquid</term>
<term>Exons</term>
<term>Humans</term>
<term>In Situ Hybridization</term>
<term>Paraffin Embedding</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">During in situ hybridisation on frozen and paraffin-embedded sections of bowel for IkappaB alpha, oligodeoxyribonucleotide probes were found to bind more avidly to eosinophils than target mRNA. This binding could not be obviated using strategies previously employed to block either binding of long DNA probes (200-mers) to eosinophils in bone marrow smears, or of riboprobes to eosinophils in sections of bowel, without removing specific hybridisation of probes. That this binding could arise through interaction of anionic oligodeoxyribonucleotides with eosinophil cationic protein, which has an unusually high pI, and is abundant in cytoplasmic granules of eosinophils, was demonstrated in vitro using real-time biomolecular interaction analysis with a BiacoreX instrument. Finally, a relationship between probe hydrophobicity, measured by reverse phase ion-pair high performance liquid chromatography, and in situ binding of individual probes to eosinophils was demonstrated. Effective tissue penetration by hydrophobic probes and subsequent strong probe-eosinophilic cationic protein interactions therefore may confound the interpretation of in situ hybridisation performed with oligonucleotide probes in eosinophil-containing tissues, such as bowel and nasal polyps.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12495221</PMID>
<DateCompleted><Year>2003</Year>
<Month>05</Month>
<Day>21</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>02</Month>
<Day>25</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0018-2214</ISSN>
<JournalIssue CitedMedium="Print"><Volume>34</Volume>
<Issue>3-4</Issue>
<PubDate><MedlineDate>2002 Mar-Apr</MedlineDate>
</PubDate>
</JournalIssue>
<Title>The Histochemical journal</Title>
<ISOAbbreviation>Histochem. J.</ISOAbbreviation>
</Journal>
<ArticleTitle>Selective binding of nucleotide probes by eosinophilic cationic protein during in situ hybridisation.</ArticleTitle>
<Pagination><MedlinePgn>153-60</MedlinePgn>
</Pagination>
<Abstract><AbstractText>During in situ hybridisation on frozen and paraffin-embedded sections of bowel for IkappaB alpha, oligodeoxyribonucleotide probes were found to bind more avidly to eosinophils than target mRNA. This binding could not be obviated using strategies previously employed to block either binding of long DNA probes (200-mers) to eosinophils in bone marrow smears, or of riboprobes to eosinophils in sections of bowel, without removing specific hybridisation of probes. That this binding could arise through interaction of anionic oligodeoxyribonucleotides with eosinophil cationic protein, which has an unusually high pI, and is abundant in cytoplasmic granules of eosinophils, was demonstrated in vitro using real-time biomolecular interaction analysis with a BiacoreX instrument. Finally, a relationship between probe hydrophobicity, measured by reverse phase ion-pair high performance liquid chromatography, and in situ binding of individual probes to eosinophils was demonstrated. Effective tissue penetration by hydrophobic probes and subsequent strong probe-eosinophilic cationic protein interactions therefore may confound the interpretation of in situ hybridisation performed with oligonucleotide probes in eosinophil-containing tissues, such as bowel and nasal polyps.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ellis</LastName>
<ForeName>Richard D</ForeName>
<Initials>RD</Initials>
<AffiliationInfo><Affiliation>Gastrointestinal Laboratory, The Rayne Institute, St. Thomas' Hospital, London, SE1 7EH, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ashwood</LastName>
<ForeName>Paul</ForeName>
<Initials>P</Initials>
</Author>
<Author ValidYN="Y"><LastName>Powell</LastName>
<ForeName>Jonathan J</ForeName>
<Initials>JJ</Initials>
</Author>
<Author ValidYN="Y"><LastName>Taylor</LastName>
<ForeName>Paul D</ForeName>
<Initials>PD</Initials>
</Author>
<Author ValidYN="Y"><LastName>Poulsom</LastName>
<ForeName>Richard</ForeName>
<Initials>R</Initials>
</Author>
<Author ValidYN="Y"><LastName>Thompson</LastName>
<ForeName>Richard P H</ForeName>
<Initials>RP</Initials>
</Author>
<Author ValidYN="Y"><LastName>Punchard</LastName>
<ForeName>Neville A</ForeName>
<Initials>NA</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>Netherlands</Country>
<MedlineTA>Histochem J</MedlineTA>
<NlmUniqueID>0163161</NlmUniqueID>
<ISSNLinking>0018-2214</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D016375">Antisense Elements (Genetics)</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D001798">Blood Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D004396">Coloring Agents</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D015342">DNA Probes</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D047091">Eosinophil Granule Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D016328">NF-kappa B</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D009711">Nucleotides</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D013439">Sulfhydryl Reagents</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014409">Tumor Necrosis Factor-alpha</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.1.-</RegistryNumber>
<NameOfSubstance UI="D012260">Ribonucleases</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>T8ID5YZU6Y</RegistryNumber>
<NameOfSubstance UI="D004229">Dithiothreitol</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D016375" MajorTopicYN="N">Antisense Elements (Genetics)</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001345" MajorTopicYN="N">Autoradiography</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001798" MajorTopicYN="N">Blood Proteins</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002851" MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003106" MajorTopicYN="N">Colon</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003111" MajorTopicYN="N">Colonic Polyps</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004396" MajorTopicYN="N">Coloring Agents</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003424" MajorTopicYN="N">Crohn Disease</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015342" MajorTopicYN="Y">DNA Probes</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004229" MajorTopicYN="N">Dithiothreitol</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D047091" MajorTopicYN="N">Eosinophil Granule Proteins</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004804" MajorTopicYN="N">Eosinophils</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005091" MajorTopicYN="N">Exons</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017403" MajorTopicYN="N">In Situ Hybridization</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016328" MajorTopicYN="N">NF-kappa B</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009711" MajorTopicYN="N">Nucleotides</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016612" MajorTopicYN="N">Paraffin Embedding</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName>
<QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012260" MajorTopicYN="Y">Ribonucleases</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013439" MajorTopicYN="N">Sulfhydryl Reagents</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014409" MajorTopicYN="N">Tumor Necrosis Factor-alpha</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2002</Year>
<Month>12</Month>
<Day>24</Day>
<Hour>4</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2003</Year>
<Month>5</Month>
<Day>22</Day>
<Hour>5</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2002</Year>
<Month>12</Month>
<Day>24</Day>
<Hour>4</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">12495221</ArticleId>
<ArticleId IdType="doi">10.1023/a:1020942531028</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Gut. 1992 Sep;33(9):1214-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1427374</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Invest. 1992 May;89(5):1662-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1569205</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 1991 Jun 28;65(7):1281-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1829648</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Invest. 1991 May;87(5):1541-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2022726</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Inflamm Bowel Dis. 1996 Spring;2(1):16-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23282452</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Pathol. 1989 Aug;158(4):279-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2475601</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol Methods. 1989 Feb;23(2):105-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2723016</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biomedicine. 1979 Feb;31(1):10-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">289422</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Infect Dis. 1989 Mar;159(3):467-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2915167</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur J Haematol. 1988 May;40(5):415-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3132400</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Gut. 1995 Aug;37(2):242-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7557575</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Am J Physiol. 1995 Jul;269(1 Pt 1):G126-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7631790</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Gut. 1995 Jul;37(1):132-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7672663</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Gut. 1995 Jul;37(1):52-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7672681</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Graefes Arch Clin Exp Ophthalmol. 1994 Nov;232(11):646-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7843589</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Histochem Cytochem. 1994 Sep;42(9):1277-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8064135</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Anal Biochem. 1993 Aug 1;212(2):351-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8214576</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Histochem Cytochem. 1993 Oct;41(10):1479-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8245407</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Histochem Cytochem. 1993 Dec;41(12):1735-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8245420</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 1996 Jun 13;381(6583):616-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8637599</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Gut. 1996 Apr;38(4):549-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8707086</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 1997 Feb 21;272(8):5275-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9030600</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Lab Anal. 1997;11(6):374-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9406060</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur J Gastroenterol Hepatol. 1998 Aug;10(8):641-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9744691</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Gut. 1998 Dec;43(6):856-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9824616</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Inflamm Res. 1998 Nov;47(11):440-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9865503</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002477 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 002477 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= PubMed |étape= Corpus |type= RBID |clé= pubmed:12495221 |texte= Selective binding of nucleotide probes by eosinophilic cationic protein during in situ hybridisation. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:12495221" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |