Characterization of a plasma membrane-resident albumin-binding protein associated with the proliferation of estrogen-target, serum-sensitive cells.
Identifieur interne : 002441 ( PubMed/Corpus ); précédent : 002440; suivant : 002442Characterization of a plasma membrane-resident albumin-binding protein associated with the proliferation of estrogen-target, serum-sensitive cells.
Auteurs : Charles E. Powell ; Ana M. Soto ; Cheryl L. Michaelson ; Fantahun Diba ; Françoise Mounier ; Pierre J. Verroust ; Carlos SonnenscheinSource :
- Steroids [ 0039-128X ] ; 2003.
English descriptors
- KwdEn :
- Blotting, Western, Cell Division (drug effects), Cell Division (physiology), Cell Line, Tumor, Estrogens (pharmacology), Humans, Immunohistochemistry, Membrane Proteins (analysis), Membrane Proteins (metabolism), Membrane Proteins (physiology), Peptide Fragments (pharmacology), Protein Binding, Protein Interaction Mapping, Receptors, Estrogen (analysis), Serum Albumin (metabolism), Serum Albumin (pharmacology).
- MESH :
- chemical , analysis : Membrane Proteins, Receptors, Estrogen.
- chemical , metabolism : Membrane Proteins, Serum Albumin.
- chemical , pharmacology : Estrogens, Peptide Fragments, Serum Albumin.
- drug effects : Cell Division.
- physiology : Cell Division, Membrane Proteins.
- Blotting, Western, Cell Line, Tumor, Humans, Immunohistochemistry, Protein Binding, Protein Interaction Mapping.
Abstract
Estrogens control the proliferation of their target cells through a receptor-mediated pathway. Recently presented evidence suggests that estradiol cancels the proliferative inhibition exerted by human albumin (HA) and recombinant human albumin (rHA) on estrogen-target serum-sensitive cells (indirect-negative hypothesis). We postulate that this mechanism requires the presence of a plasma membrane estrogen receptor (mER) and a plasma membrane albumin-binding protein (mABP). Direct evidence confirming the presence of mERalpha in MCF7 cells has recently been presented. Herein, we now show that Western blot analysis of purified T47D membrane proteins with the C542 ERalpha specific monoclonal antibody also revealed specific, multiple M(r) mERs (67, 110, and 130k M(r)). In addition, Western blot analysis with an ABP antiserum revealed a potential 60k M(r) ABP in both MCF7 and T47D plasma membrane extracts. No such evidence was observed in similar extracts from ER-negative, serum-insensitive MDA-MB231 cells. Ligand blot analysis of similar plasma membrane extracts with bovine serum albumin confirmed the presence of a 60k M(r) ABP in MCF7 and T47D cells; again, no such evidence was observed in comparable extracts from MDA-MB231 cells. Fluorescence and confocal microscopy of MCF7 cells fixed in 2.0% paraformaldehyde/0.1% glutaraldehyde identified specific membrane ABP antigenic sites by immunocytochemistry. Serum-insensitive MDA-MB231 cells fixed and labeled similarly did not exhibit this mABP. These results suggest that the proposed mABP is expressed only in serum-sensitive estrogen-target cells and is not expressed in cells insensitive to the proliferative inhibition of HA and rHA. Also, the present data suggest that the proposed mABP may be the recognition mechanism by which both HA and rHA inhibit MCF7 and T47D cell proliferation.
DOI: 10.1016/s0039-128x(03)00047-3
PubMed: 12906933
Links to Exploration step
pubmed:12906933Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization of a plasma membrane-resident albumin-binding protein associated with the proliferation of estrogen-target, serum-sensitive cells.</title><author><name sortKey="Powell, Charles E" sort="Powell, Charles E" uniqKey="Powell C" first="Charles E" last="Powell">Charles E. Powell</name><affiliation><nlm:affiliation>Department of Anatomy and Cellular Biology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Soto, Ana M" sort="Soto, Ana M" uniqKey="Soto A" first="Ana M" last="Soto">Ana M. Soto</name></author><author><name sortKey="Michaelson, Cheryl L" sort="Michaelson, Cheryl L" uniqKey="Michaelson C" first="Cheryl L" last="Michaelson">Cheryl L. Michaelson</name></author><author><name sortKey="Diba, Fantahun" sort="Diba, Fantahun" uniqKey="Diba F" first="Fantahun" last="Diba">Fantahun Diba</name></author><author><name sortKey="Mounier, Francoise" sort="Mounier, Francoise" uniqKey="Mounier F" first="Françoise" last="Mounier">Françoise Mounier</name></author><author><name sortKey="Verroust, Pierre J" sort="Verroust, Pierre J" uniqKey="Verroust P" first="Pierre J" last="Verroust">Pierre J. Verroust</name></author><author><name sortKey="Sonnenschein, Carlos" sort="Sonnenschein, Carlos" uniqKey="Sonnenschein C" first="Carlos" last="Sonnenschein">Carlos Sonnenschein</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:12906933</idno><idno type="pmid">12906933</idno><idno type="doi">10.1016/s0039-128x(03)00047-3</idno><idno type="wicri:Area/PubMed/Corpus">002441</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002441</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Characterization of a plasma membrane-resident albumin-binding protein associated with the proliferation of estrogen-target, serum-sensitive cells.</title><author><name sortKey="Powell, Charles E" sort="Powell, Charles E" uniqKey="Powell C" first="Charles E" last="Powell">Charles E. Powell</name><affiliation><nlm:affiliation>Department of Anatomy and Cellular Biology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Soto, Ana M" sort="Soto, Ana M" uniqKey="Soto A" first="Ana M" last="Soto">Ana M. Soto</name></author><author><name sortKey="Michaelson, Cheryl L" sort="Michaelson, Cheryl L" uniqKey="Michaelson C" first="Cheryl L" last="Michaelson">Cheryl L. Michaelson</name></author><author><name sortKey="Diba, Fantahun" sort="Diba, Fantahun" uniqKey="Diba F" first="Fantahun" last="Diba">Fantahun Diba</name></author><author><name sortKey="Mounier, Francoise" sort="Mounier, Francoise" uniqKey="Mounier F" first="Françoise" last="Mounier">Françoise Mounier</name></author><author><name sortKey="Verroust, Pierre J" sort="Verroust, Pierre J" uniqKey="Verroust P" first="Pierre J" last="Verroust">Pierre J. Verroust</name></author><author><name sortKey="Sonnenschein, Carlos" sort="Sonnenschein, Carlos" uniqKey="Sonnenschein C" first="Carlos" last="Sonnenschein">Carlos Sonnenschein</name></author></analytic><series><title level="j">Steroids</title><idno type="ISSN">0039-128X</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Blotting, Western</term><term>Cell Division (drug effects)</term><term>Cell Division (physiology)</term><term>Cell Line, Tumor</term><term>Estrogens (pharmacology)</term><term>Humans</term><term>Immunohistochemistry</term><term>Membrane Proteins (analysis)</term><term>Membrane Proteins (metabolism)</term><term>Membrane Proteins (physiology)</term><term>Peptide Fragments (pharmacology)</term><term>Protein Binding</term><term>Protein Interaction Mapping</term><term>Receptors, Estrogen (analysis)</term><term>Serum Albumin (metabolism)</term><term>Serum Albumin (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Membrane Proteins</term><term>Receptors, Estrogen</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Proteins</term><term>Serum Albumin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Estrogens</term><term>Peptide Fragments</term><term>Serum Albumin</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Division</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Division</term><term>Membrane Proteins</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Western</term><term>Cell Line, Tumor</term><term>Humans</term><term>Immunohistochemistry</term><term>Protein Binding</term><term>Protein Interaction Mapping</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Estrogens control the proliferation of their target cells through a receptor-mediated pathway. Recently presented evidence suggests that estradiol cancels the proliferative inhibition exerted by human albumin (HA) and recombinant human albumin (rHA) on estrogen-target serum-sensitive cells (indirect-negative hypothesis). We postulate that this mechanism requires the presence of a plasma membrane estrogen receptor (mER) and a plasma membrane albumin-binding protein (mABP). Direct evidence confirming the presence of mERalpha in MCF7 cells has recently been presented. Herein, we now show that Western blot analysis of purified T47D membrane proteins with the C542 ERalpha specific monoclonal antibody also revealed specific, multiple M(r) mERs (67, 110, and 130k M(r)). In addition, Western blot analysis with an ABP antiserum revealed a potential 60k M(r) ABP in both MCF7 and T47D plasma membrane extracts. No such evidence was observed in similar extracts from ER-negative, serum-insensitive MDA-MB231 cells. Ligand blot analysis of similar plasma membrane extracts with bovine serum albumin confirmed the presence of a 60k M(r) ABP in MCF7 and T47D cells; again, no such evidence was observed in comparable extracts from MDA-MB231 cells. Fluorescence and confocal microscopy of MCF7 cells fixed in 2.0% paraformaldehyde/0.1% glutaraldehyde identified specific membrane ABP antigenic sites by immunocytochemistry. Serum-insensitive MDA-MB231 cells fixed and labeled similarly did not exhibit this mABP. These results suggest that the proposed mABP is expressed only in serum-sensitive estrogen-target cells and is not expressed in cells insensitive to the proliferative inhibition of HA and rHA. Also, the present data suggest that the proposed mABP may be the recognition mechanism by which both HA and rHA inhibit MCF7 and T47D cell proliferation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12906933</PMID><DateCompleted><Year>2004</Year><Month>05</Month><Day>19</Day></DateCompleted><DateRevised><Year>2019</Year><Month>08</Month><Day>23</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0039-128X</ISSN><JournalIssue CitedMedium="Print"><Volume>68</Volume><Issue>6</Issue><PubDate><Year>2003</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Steroids</Title><ISOAbbreviation>Steroids</ISOAbbreviation></Journal><ArticleTitle>Characterization of a plasma membrane-resident albumin-binding protein associated with the proliferation of estrogen-target, serum-sensitive cells.</ArticleTitle><Pagination><MedlinePgn>487-96</MedlinePgn></Pagination><Abstract><AbstractText>Estrogens control the proliferation of their target cells through a receptor-mediated pathway. Recently presented evidence suggests that estradiol cancels the proliferative inhibition exerted by human albumin (HA) and recombinant human albumin (rHA) on estrogen-target serum-sensitive cells (indirect-negative hypothesis). We postulate that this mechanism requires the presence of a plasma membrane estrogen receptor (mER) and a plasma membrane albumin-binding protein (mABP). Direct evidence confirming the presence of mERalpha in MCF7 cells has recently been presented. Herein, we now show that Western blot analysis of purified T47D membrane proteins with the C542 ERalpha specific monoclonal antibody also revealed specific, multiple M(r) mERs (67, 110, and 130k M(r)). In addition, Western blot analysis with an ABP antiserum revealed a potential 60k M(r) ABP in both MCF7 and T47D plasma membrane extracts. No such evidence was observed in similar extracts from ER-negative, serum-insensitive MDA-MB231 cells. Ligand blot analysis of similar plasma membrane extracts with bovine serum albumin confirmed the presence of a 60k M(r) ABP in MCF7 and T47D cells; again, no such evidence was observed in comparable extracts from MDA-MB231 cells. Fluorescence and confocal microscopy of MCF7 cells fixed in 2.0% paraformaldehyde/0.1% glutaraldehyde identified specific membrane ABP antigenic sites by immunocytochemistry. Serum-insensitive MDA-MB231 cells fixed and labeled similarly did not exhibit this mABP. These results suggest that the proposed mABP is expressed only in serum-sensitive estrogen-target cells and is not expressed in cells insensitive to the proliferative inhibition of HA and rHA. Also, the present data suggest that the proposed mABP may be the recognition mechanism by which both HA and rHA inhibit MCF7 and T47D cell proliferation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Powell</LastName><ForeName>Charles E</ForeName><Initials>CE</Initials><AffiliationInfo><Affiliation>Department of Anatomy and Cellular Biology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Soto</LastName><ForeName>Ana M</ForeName><Initials>AM</Initials></Author><Author ValidYN="Y"><LastName>Michaelson</LastName><ForeName>Cheryl L</ForeName><Initials>CL</Initials></Author><Author ValidYN="Y"><LastName>Diba</LastName><ForeName>Fantahun</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Mounier</LastName><ForeName>Françoise</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Verroust</LastName><ForeName>Pierre J</ForeName><Initials>PJ</Initials></Author><Author ValidYN="Y"><LastName>Sonnenschein</LastName><ForeName>Carlos</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>CA13410</GrantID><Acronym>CA</Acronym><Agency>NCI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>CA55574</GrantID><Acronym>CA</Acronym><Agency>NCI NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Steroids</MedlineTA><NlmUniqueID>0404536</NlmUniqueID><ISSNLinking>0039-128X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance 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