Minimal T-cell-stimulatory sequences and spectrum of HLA restriction of immunodominant CD4+ T-cell epitopes within hepatitis C virus NS3 and NS4 proteins.
Identifieur interne : 002311 ( PubMed/Corpus ); précédent : 002310; suivant : 002312Minimal T-cell-stimulatory sequences and spectrum of HLA restriction of immunodominant CD4+ T-cell epitopes within hepatitis C virus NS3 and NS4 proteins.
Auteurs : J T Gerlach ; A. Ulsenheimer ; N H Grüner ; M-C Jung ; W. Schraut ; C-A Schirren ; M. Heeg ; S. Scholz ; K. Witter ; R. Zahn ; A. Vogler ; R. Zachoval ; G R Pape ; H M DiepolderSource :
- Journal of virology [ 0022-538X ] ; 2005.
English descriptors
- KwdEn :
- Adolescent, Adult, Alleles, Amino Acid Sequence, Antigen Presentation, CD4-Positive T-Lymphocytes (immunology), Female, HLA Antigens (genetics), HLA Antigens (physiology), Hepacivirus (immunology), Hepatitis C (immunology), Humans, Immunodominant Epitopes, Leukocytes, Mononuclear (immunology), Lymphocyte Activation, Male, Middle Aged, Molecular Sequence Data, Viral Nonstructural Proteins (immunology).
- MESH :
- chemical , genetics : HLA Antigens.
- immunology : CD4-Positive T-Lymphocytes, Hepacivirus, Hepatitis C, Leukocytes, Mononuclear, Viral Nonstructural Proteins.
- chemical , physiology : HLA Antigens.
- Adolescent, Adult, Alleles, Amino Acid Sequence, Antigen Presentation, Female, Humans, Immunodominant Epitopes, Lymphocyte Activation, Male, Middle Aged, Molecular Sequence Data.
Abstract
The hepatitis C virus (HCV)-specific CD4+ T-cell response against nonstructural proteins is strongly associated with successful viral clearance during acute hepatitis C. To further develop these observations into peptide-based vaccines and clinical immunomonitoring tools like HLA class II tetramers, a detailed characterization of immunodominant CD4+ T-cell epitopes is required. We studied peripheral blood mononuclear cells from 20 patients with acute hepatitis C using 83 overlapping 20-mer peptides covering the NS3 helicase and NS4. Eight peptides were recognized by > or = 40% of patients, and specific CD4+ T-cell clones were obtained for seven of these and three additional, subdominant epitopes. Mapping of minimal stimulatory sequences defined epitopes of 8 to 13 amino acids in length, but optimal T-cell stimulation was observed with 10- to 15-mers. While some epitopes were presented by different HLA molecules, others were presented by only a single HLA class II molecule, which has implications for patient selection in clinical trials of peptide-based immunotherapies. In conclusion, using two different approaches we identified and characterized a set of CD4+ T-cell epitopes in the HCV NS3-NS4 region which are immunodominant in patients achieving transient or persistent viral control. This information allows the construction of a valuable panel of HCV-specific HLA class II tetramers for further study of CD4+ T-cell responses in chronic hepatitis C. The finding of immunodominant epitopes with very constrained HLA restriction has implications for patient selection in clinical trials of peptide-based immunotherapies.
DOI: 10.1128/JVI.79.19.12425-12433.2005
PubMed: 16160170
Links to Exploration step
pubmed:16160170Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Minimal T-cell-stimulatory sequences and spectrum of HLA restriction of immunodominant CD4+ T-cell epitopes within hepatitis C virus NS3 and NS4 proteins.</title>
<author><name sortKey="Gerlach, J T" sort="Gerlach, J T" uniqKey="Gerlach J" first="J T" last="Gerlach">J T Gerlach</name>
<affiliation><nlm:affiliation>Department of Gastroenterology and Hepatology, University Hospital Zurich, Rämistrasse 100, 8091 Zurich, Switzerland. Tilman.Gerlach@usz.ch</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Ulsenheimer, A" sort="Ulsenheimer, A" uniqKey="Ulsenheimer A" first="A" last="Ulsenheimer">A. Ulsenheimer</name>
</author>
<author><name sortKey="Gruner, N H" sort="Gruner, N H" uniqKey="Gruner N" first="N H" last="Grüner">N H Grüner</name>
</author>
<author><name sortKey="Jung, M C" sort="Jung, M C" uniqKey="Jung M" first="M-C" last="Jung">M-C Jung</name>
</author>
<author><name sortKey="Schraut, W" sort="Schraut, W" uniqKey="Schraut W" first="W" last="Schraut">W. Schraut</name>
</author>
<author><name sortKey="Schirren, C A" sort="Schirren, C A" uniqKey="Schirren C" first="C-A" last="Schirren">C-A Schirren</name>
</author>
<author><name sortKey="Heeg, M" sort="Heeg, M" uniqKey="Heeg M" first="M" last="Heeg">M. Heeg</name>
</author>
<author><name sortKey="Scholz, S" sort="Scholz, S" uniqKey="Scholz S" first="S" last="Scholz">S. Scholz</name>
</author>
<author><name sortKey="Witter, K" sort="Witter, K" uniqKey="Witter K" first="K" last="Witter">K. Witter</name>
</author>
<author><name sortKey="Zahn, R" sort="Zahn, R" uniqKey="Zahn R" first="R" last="Zahn">R. Zahn</name>
</author>
<author><name sortKey="Vogler, A" sort="Vogler, A" uniqKey="Vogler A" first="A" last="Vogler">A. Vogler</name>
</author>
<author><name sortKey="Zachoval, R" sort="Zachoval, R" uniqKey="Zachoval R" first="R" last="Zachoval">R. Zachoval</name>
</author>
<author><name sortKey="Pape, G R" sort="Pape, G R" uniqKey="Pape G" first="G R" last="Pape">G R Pape</name>
</author>
<author><name sortKey="Diepolder, H M" sort="Diepolder, H M" uniqKey="Diepolder H" first="H M" last="Diepolder">H M Diepolder</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2005">2005</date>
<idno type="RBID">pubmed:16160170</idno>
<idno type="pmid">16160170</idno>
<idno type="doi">10.1128/JVI.79.19.12425-12433.2005</idno>
<idno type="wicri:Area/PubMed/Corpus">002311</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002311</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Minimal T-cell-stimulatory sequences and spectrum of HLA restriction of immunodominant CD4+ T-cell epitopes within hepatitis C virus NS3 and NS4 proteins.</title>
<author><name sortKey="Gerlach, J T" sort="Gerlach, J T" uniqKey="Gerlach J" first="J T" last="Gerlach">J T Gerlach</name>
<affiliation><nlm:affiliation>Department of Gastroenterology and Hepatology, University Hospital Zurich, Rämistrasse 100, 8091 Zurich, Switzerland. Tilman.Gerlach@usz.ch</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Ulsenheimer, A" sort="Ulsenheimer, A" uniqKey="Ulsenheimer A" first="A" last="Ulsenheimer">A. Ulsenheimer</name>
</author>
<author><name sortKey="Gruner, N H" sort="Gruner, N H" uniqKey="Gruner N" first="N H" last="Grüner">N H Grüner</name>
</author>
<author><name sortKey="Jung, M C" sort="Jung, M C" uniqKey="Jung M" first="M-C" last="Jung">M-C Jung</name>
</author>
<author><name sortKey="Schraut, W" sort="Schraut, W" uniqKey="Schraut W" first="W" last="Schraut">W. Schraut</name>
</author>
<author><name sortKey="Schirren, C A" sort="Schirren, C A" uniqKey="Schirren C" first="C-A" last="Schirren">C-A Schirren</name>
</author>
<author><name sortKey="Heeg, M" sort="Heeg, M" uniqKey="Heeg M" first="M" last="Heeg">M. Heeg</name>
</author>
<author><name sortKey="Scholz, S" sort="Scholz, S" uniqKey="Scholz S" first="S" last="Scholz">S. Scholz</name>
</author>
<author><name sortKey="Witter, K" sort="Witter, K" uniqKey="Witter K" first="K" last="Witter">K. Witter</name>
</author>
<author><name sortKey="Zahn, R" sort="Zahn, R" uniqKey="Zahn R" first="R" last="Zahn">R. Zahn</name>
</author>
<author><name sortKey="Vogler, A" sort="Vogler, A" uniqKey="Vogler A" first="A" last="Vogler">A. Vogler</name>
</author>
<author><name sortKey="Zachoval, R" sort="Zachoval, R" uniqKey="Zachoval R" first="R" last="Zachoval">R. Zachoval</name>
</author>
<author><name sortKey="Pape, G R" sort="Pape, G R" uniqKey="Pape G" first="G R" last="Pape">G R Pape</name>
</author>
<author><name sortKey="Diepolder, H M" sort="Diepolder, H M" uniqKey="Diepolder H" first="H M" last="Diepolder">H M Diepolder</name>
</author>
</analytic>
<series><title level="j">Journal of virology</title>
<idno type="ISSN">0022-538X</idno>
<imprint><date when="2005" type="published">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term>
<term>Adult</term>
<term>Alleles</term>
<term>Amino Acid Sequence</term>
<term>Antigen Presentation</term>
<term>CD4-Positive T-Lymphocytes (immunology)</term>
<term>Female</term>
<term>HLA Antigens (genetics)</term>
<term>HLA Antigens (physiology)</term>
<term>Hepacivirus (immunology)</term>
<term>Hepatitis C (immunology)</term>
<term>Humans</term>
<term>Immunodominant Epitopes</term>
<term>Leukocytes, Mononuclear (immunology)</term>
<term>Lymphocyte Activation</term>
<term>Male</term>
<term>Middle Aged</term>
<term>Molecular Sequence Data</term>
<term>Viral Nonstructural Proteins (immunology)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>HLA Antigens</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>CD4-Positive T-Lymphocytes</term>
<term>Hepacivirus</term>
<term>Hepatitis C</term>
<term>Leukocytes, Mononuclear</term>
<term>Viral Nonstructural Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>HLA Antigens</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Adolescent</term>
<term>Adult</term>
<term>Alleles</term>
<term>Amino Acid Sequence</term>
<term>Antigen Presentation</term>
<term>Female</term>
<term>Humans</term>
<term>Immunodominant Epitopes</term>
<term>Lymphocyte Activation</term>
<term>Male</term>
<term>Middle Aged</term>
<term>Molecular Sequence Data</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The hepatitis C virus (HCV)-specific CD4+ T-cell response against nonstructural proteins is strongly associated with successful viral clearance during acute hepatitis C. To further develop these observations into peptide-based vaccines and clinical immunomonitoring tools like HLA class II tetramers, a detailed characterization of immunodominant CD4+ T-cell epitopes is required. We studied peripheral blood mononuclear cells from 20 patients with acute hepatitis C using 83 overlapping 20-mer peptides covering the NS3 helicase and NS4. Eight peptides were recognized by > or = 40% of patients, and specific CD4+ T-cell clones were obtained for seven of these and three additional, subdominant epitopes. Mapping of minimal stimulatory sequences defined epitopes of 8 to 13 amino acids in length, but optimal T-cell stimulation was observed with 10- to 15-mers. While some epitopes were presented by different HLA molecules, others were presented by only a single HLA class II molecule, which has implications for patient selection in clinical trials of peptide-based immunotherapies. In conclusion, using two different approaches we identified and characterized a set of CD4+ T-cell epitopes in the HCV NS3-NS4 region which are immunodominant in patients achieving transient or persistent viral control. This information allows the construction of a valuable panel of HCV-specific HLA class II tetramers for further study of CD4+ T-cell responses in chronic hepatitis C. The finding of immunodominant epitopes with very constrained HLA restriction has implications for patient selection in clinical trials of peptide-based immunotherapies.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16160170</PMID>
<DateCompleted><Year>2005</Year>
<Month>10</Month>
<Day>21</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-538X</ISSN>
<JournalIssue CitedMedium="Print"><Volume>79</Volume>
<Issue>19</Issue>
<PubDate><Year>2005</Year>
<Month>Oct</Month>
</PubDate>
</JournalIssue>
<Title>Journal of virology</Title>
<ISOAbbreviation>J. Virol.</ISOAbbreviation>
</Journal>
<ArticleTitle>Minimal T-cell-stimulatory sequences and spectrum of HLA restriction of immunodominant CD4+ T-cell epitopes within hepatitis C virus NS3 and NS4 proteins.</ArticleTitle>
<Pagination><MedlinePgn>12425-33</MedlinePgn>
</Pagination>
<Abstract><AbstractText>The hepatitis C virus (HCV)-specific CD4+ T-cell response against nonstructural proteins is strongly associated with successful viral clearance during acute hepatitis C. To further develop these observations into peptide-based vaccines and clinical immunomonitoring tools like HLA class II tetramers, a detailed characterization of immunodominant CD4+ T-cell epitopes is required. We studied peripheral blood mononuclear cells from 20 patients with acute hepatitis C using 83 overlapping 20-mer peptides covering the NS3 helicase and NS4. Eight peptides were recognized by > or = 40% of patients, and specific CD4+ T-cell clones were obtained for seven of these and three additional, subdominant epitopes. Mapping of minimal stimulatory sequences defined epitopes of 8 to 13 amino acids in length, but optimal T-cell stimulation was observed with 10- to 15-mers. While some epitopes were presented by different HLA molecules, others were presented by only a single HLA class II molecule, which has implications for patient selection in clinical trials of peptide-based immunotherapies. In conclusion, using two different approaches we identified and characterized a set of CD4+ T-cell epitopes in the HCV NS3-NS4 region which are immunodominant in patients achieving transient or persistent viral control. This information allows the construction of a valuable panel of HCV-specific HLA class II tetramers for further study of CD4+ T-cell responses in chronic hepatitis C. The finding of immunodominant epitopes with very constrained HLA restriction has implications for patient selection in clinical trials of peptide-based immunotherapies.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gerlach</LastName>
<ForeName>J T</ForeName>
<Initials>JT</Initials>
<AffiliationInfo><Affiliation>Department of Gastroenterology and Hepatology, University Hospital Zurich, Rämistrasse 100, 8091 Zurich, Switzerland. Tilman.Gerlach@usz.ch</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ulsenheimer</LastName>
<ForeName>A</ForeName>
<Initials>A</Initials>
</Author>
<Author ValidYN="Y"><LastName>Grüner</LastName>
<ForeName>N H</ForeName>
<Initials>NH</Initials>
</Author>
<Author ValidYN="Y"><LastName>Jung</LastName>
<ForeName>M-C</ForeName>
<Initials>MC</Initials>
</Author>
<Author ValidYN="Y"><LastName>Schraut</LastName>
<ForeName>W</ForeName>
<Initials>W</Initials>
</Author>
<Author ValidYN="Y"><LastName>Schirren</LastName>
<ForeName>C-A</ForeName>
<Initials>CA</Initials>
</Author>
<Author ValidYN="Y"><LastName>Heeg</LastName>
<ForeName>M</ForeName>
<Initials>M</Initials>
</Author>
<Author ValidYN="Y"><LastName>Scholz</LastName>
<ForeName>S</ForeName>
<Initials>S</Initials>
</Author>
<Author ValidYN="Y"><LastName>Witter</LastName>
<ForeName>K</ForeName>
<Initials>K</Initials>
</Author>
<Author ValidYN="Y"><LastName>Zahn</LastName>
<ForeName>R</ForeName>
<Initials>R</Initials>
</Author>
<Author ValidYN="Y"><LastName>Vogler</LastName>
<ForeName>A</ForeName>
<Initials>A</Initials>
</Author>
<Author ValidYN="Y"><LastName>Zachoval</LastName>
<ForeName>R</ForeName>
<Initials>R</Initials>
</Author>
<Author ValidYN="Y"><LastName>Pape</LastName>
<ForeName>G R</ForeName>
<Initials>GR</Initials>
</Author>
<Author ValidYN="Y"><LastName>Diepolder</LastName>
<ForeName>H M</ForeName>
<Initials>HM</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>J Virol</MedlineTA>
<NlmUniqueID>0113724</NlmUniqueID>
<ISSNLinking>0022-538X</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D006680">HLA Antigens</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D016056">Immunodominant Epitopes</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C084422">NS3 protein, hepatitis C virus</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C093542">NS4 protein, hepatitis C virus</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D017361">Viral Nonstructural Proteins</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000293" MajorTopicYN="N">Adolescent</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000483" MajorTopicYN="N">Alleles</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017951" MajorTopicYN="N">Antigen Presentation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015496" MajorTopicYN="N">CD4-Positive T-Lymphocytes</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006680" MajorTopicYN="N">HLA Antigens</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016174" MajorTopicYN="N">Hepacivirus</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006526" MajorTopicYN="N">Hepatitis C</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016056" MajorTopicYN="Y">Immunodominant Epitopes</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007963" MajorTopicYN="N">Leukocytes, Mononuclear</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008213" MajorTopicYN="N">Lymphocyte Activation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017361" MajorTopicYN="N">Viral Nonstructural Proteins</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year>
<Month>9</Month>
<Day>15</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2005</Year>
<Month>10</Month>
<Day>22</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2005</Year>
<Month>9</Month>
<Day>15</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">16160170</ArticleId>
<ArticleId IdType="pii">79/19/12425</ArticleId>
<ArticleId IdType="doi">10.1128/JVI.79.19.12425-12433.2005</ArticleId>
<ArticleId IdType="pmc">PMC1211510</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>J Hepatol. 1999;31 Suppl 1:88-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10622567</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Gastroenterology. 1999 Oct;117(4):933-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10500077</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Semin Liver Dis. 2000;20(1):103-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10895435</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur J Immunol. 2001 May;31(5):1438-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11465100</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Med. 2001 Nov 19;194(10):1395-406</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11714747</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Hepatology. 2002 May;35(5):1225-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11981773</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2002 Dec;76(24):12584-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12438584</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Hepatology. 2003 Mar;37(3):577-89</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12601356</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Immunogenetics. 2004 Mar;55(12):797-810</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14963618</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Viral Hepat. 2004 Jul;11(4):324-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15230855</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Hepatology. 2004 Jul;40(1):87-97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15239090</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Hepatology. 1995 Mar;21(3):632-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7875660</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 1995 Oct 14;346(8981):1006-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7475549</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Invest. 1996 Aug 1;98(3):706-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8698862</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Hepatology. 1997 Feb;25(2):449-58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9021963</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 1997 Aug;71(8):6011-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9223492</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int Immunol. 1999 Apr;11(4):577-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10323211</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Hepatology. 1999 Oct;30(4):1088-98</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10498664</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Med. 2000 May 1;191(9):1499-512</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10790425</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002311 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 002311 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= PubMed |étape= Corpus |type= RBID |clé= pubmed:16160170 |texte= Minimal T-cell-stimulatory sequences and spectrum of HLA restriction of immunodominant CD4+ T-cell epitopes within hepatitis C virus NS3 and NS4 proteins. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:16160170" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |