Minimal T-cell-stimulatory sequences and spectrum of HLA restriction of immunodominant CD4+ T-cell epitopes within hepatitis C virus NS3 and NS4 proteins.
Identifieur interne : 002311 ( PubMed/Corpus ); précédent : 002310; suivant : 002312Minimal T-cell-stimulatory sequences and spectrum of HLA restriction of immunodominant CD4+ T-cell epitopes within hepatitis C virus NS3 and NS4 proteins.
Auteurs : J T Gerlach ; A. Ulsenheimer ; N H Grüner ; M-C Jung ; W. Schraut ; C-A Schirren ; M. Heeg ; S. Scholz ; K. Witter ; R. Zahn ; A. Vogler ; R. Zachoval ; G R Pape ; H M DiepolderSource :
- Journal of virology [ 0022-538X ] ; 2005.
English descriptors
- KwdEn :
- Adolescent, Adult, Alleles, Amino Acid Sequence, Antigen Presentation, CD4-Positive T-Lymphocytes (immunology), Female, HLA Antigens (genetics), HLA Antigens (physiology), Hepacivirus (immunology), Hepatitis C (immunology), Humans, Immunodominant Epitopes, Leukocytes, Mononuclear (immunology), Lymphocyte Activation, Male, Middle Aged, Molecular Sequence Data, Viral Nonstructural Proteins (immunology).
- MESH :
- chemical , genetics : HLA Antigens.
- immunology : CD4-Positive T-Lymphocytes, Hepacivirus, Hepatitis C, Leukocytes, Mononuclear, Viral Nonstructural Proteins.
- chemical , physiology : HLA Antigens.
- Adolescent, Adult, Alleles, Amino Acid Sequence, Antigen Presentation, Female, Humans, Immunodominant Epitopes, Lymphocyte Activation, Male, Middle Aged, Molecular Sequence Data.
Abstract
The hepatitis C virus (HCV)-specific CD4+ T-cell response against nonstructural proteins is strongly associated with successful viral clearance during acute hepatitis C. To further develop these observations into peptide-based vaccines and clinical immunomonitoring tools like HLA class II tetramers, a detailed characterization of immunodominant CD4+ T-cell epitopes is required. We studied peripheral blood mononuclear cells from 20 patients with acute hepatitis C using 83 overlapping 20-mer peptides covering the NS3 helicase and NS4. Eight peptides were recognized by > or = 40% of patients, and specific CD4+ T-cell clones were obtained for seven of these and three additional, subdominant epitopes. Mapping of minimal stimulatory sequences defined epitopes of 8 to 13 amino acids in length, but optimal T-cell stimulation was observed with 10- to 15-mers. While some epitopes were presented by different HLA molecules, others were presented by only a single HLA class II molecule, which has implications for patient selection in clinical trials of peptide-based immunotherapies. In conclusion, using two different approaches we identified and characterized a set of CD4+ T-cell epitopes in the HCV NS3-NS4 region which are immunodominant in patients achieving transient or persistent viral control. This information allows the construction of a valuable panel of HCV-specific HLA class II tetramers for further study of CD4+ T-cell responses in chronic hepatitis C. The finding of immunodominant epitopes with very constrained HLA restriction has implications for patient selection in clinical trials of peptide-based immunotherapies.
DOI: 10.1128/JVI.79.19.12425-12433.2005
PubMed: 16160170
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Ulsenheimer</name></author><author><name sortKey="Gruner, N H" sort="Gruner, N H" uniqKey="Gruner N" first="N H" last="Grüner">N H Grüner</name></author><author><name sortKey="Jung, M C" sort="Jung, M C" uniqKey="Jung M" first="M-C" last="Jung">M-C Jung</name></author><author><name sortKey="Schraut, W" sort="Schraut, W" uniqKey="Schraut W" first="W" last="Schraut">W. Schraut</name></author><author><name sortKey="Schirren, C A" sort="Schirren, C A" uniqKey="Schirren C" first="C-A" last="Schirren">C-A Schirren</name></author><author><name sortKey="Heeg, M" sort="Heeg, M" uniqKey="Heeg M" first="M" last="Heeg">M. Heeg</name></author><author><name sortKey="Scholz, S" sort="Scholz, S" uniqKey="Scholz S" first="S" last="Scholz">S. Scholz</name></author><author><name sortKey="Witter, K" sort="Witter, K" uniqKey="Witter K" first="K" last="Witter">K. Witter</name></author><author><name sortKey="Zahn, R" sort="Zahn, R" uniqKey="Zahn R" first="R" last="Zahn">R. 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Tilman.Gerlach@usz.ch</nlm:affiliation></affiliation></author><author><name sortKey="Ulsenheimer, A" sort="Ulsenheimer, A" uniqKey="Ulsenheimer A" first="A" last="Ulsenheimer">A. Ulsenheimer</name></author><author><name sortKey="Gruner, N H" sort="Gruner, N H" uniqKey="Gruner N" first="N H" last="Grüner">N H Grüner</name></author><author><name sortKey="Jung, M C" sort="Jung, M C" uniqKey="Jung M" first="M-C" last="Jung">M-C Jung</name></author><author><name sortKey="Schraut, W" sort="Schraut, W" uniqKey="Schraut W" first="W" last="Schraut">W. Schraut</name></author><author><name sortKey="Schirren, C A" sort="Schirren, C A" uniqKey="Schirren C" first="C-A" last="Schirren">C-A Schirren</name></author><author><name sortKey="Heeg, M" sort="Heeg, M" uniqKey="Heeg M" first="M" last="Heeg">M. Heeg</name></author><author><name sortKey="Scholz, S" sort="Scholz, S" uniqKey="Scholz S" first="S" last="Scholz">S. Scholz</name></author><author><name sortKey="Witter, K" sort="Witter, K" uniqKey="Witter K" first="K" last="Witter">K. Witter</name></author><author><name sortKey="Zahn, R" sort="Zahn, R" uniqKey="Zahn R" first="R" last="Zahn">R. Zahn</name></author><author><name sortKey="Vogler, A" sort="Vogler, A" uniqKey="Vogler A" first="A" last="Vogler">A. Vogler</name></author><author><name sortKey="Zachoval, R" sort="Zachoval, R" uniqKey="Zachoval R" first="R" last="Zachoval">R. Zachoval</name></author><author><name sortKey="Pape, G R" sort="Pape, G R" uniqKey="Pape G" first="G R" last="Pape">G R Pape</name></author><author><name sortKey="Diepolder, H M" sort="Diepolder, H M" uniqKey="Diepolder H" first="H M" last="Diepolder">H M Diepolder</name></author></analytic><series><title level="j">Journal of virology</title><idno type="ISSN">0022-538X</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Alleles</term><term>Amino Acid Sequence</term><term>Antigen Presentation</term><term>CD4-Positive T-Lymphocytes (immunology)</term><term>Female</term><term>HLA Antigens (genetics)</term><term>HLA Antigens (physiology)</term><term>Hepacivirus (immunology)</term><term>Hepatitis C (immunology)</term><term>Humans</term><term>Immunodominant Epitopes</term><term>Leukocytes, Mononuclear (immunology)</term><term>Lymphocyte Activation</term><term>Male</term><term>Middle Aged</term><term>Molecular Sequence Data</term><term>Viral Nonstructural Proteins (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>HLA Antigens</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>CD4-Positive T-Lymphocytes</term><term>Hepacivirus</term><term>Hepatitis C</term><term>Leukocytes, Mononuclear</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>HLA Antigens</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Alleles</term><term>Amino Acid Sequence</term><term>Antigen Presentation</term><term>Female</term><term>Humans</term><term>Immunodominant Epitopes</term><term>Lymphocyte Activation</term><term>Male</term><term>Middle Aged</term><term>Molecular Sequence Data</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The hepatitis C virus (HCV)-specific CD4+ T-cell response against nonstructural proteins is strongly associated with successful viral clearance during acute hepatitis C. To further develop these observations into peptide-based vaccines and clinical immunomonitoring tools like HLA class II tetramers, a detailed characterization of immunodominant CD4+ T-cell epitopes is required. We studied peripheral blood mononuclear cells from 20 patients with acute hepatitis C using 83 overlapping 20-mer peptides covering the NS3 helicase and NS4. Eight peptides were recognized by > or = 40% of patients, and specific CD4+ T-cell clones were obtained for seven of these and three additional, subdominant epitopes. Mapping of minimal stimulatory sequences defined epitopes of 8 to 13 amino acids in length, but optimal T-cell stimulation was observed with 10- to 15-mers. While some epitopes were presented by different HLA molecules, others were presented by only a single HLA class II molecule, which has implications for patient selection in clinical trials of peptide-based immunotherapies. In conclusion, using two different approaches we identified and characterized a set of CD4+ T-cell epitopes in the HCV NS3-NS4 region which are immunodominant in patients achieving transient or persistent viral control. This information allows the construction of a valuable panel of HCV-specific HLA class II tetramers for further study of CD4+ T-cell responses in chronic hepatitis C. The finding of immunodominant epitopes with very constrained HLA restriction has implications for patient selection in clinical trials of peptide-based immunotherapies.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16160170</PMID><DateCompleted><Year>2005</Year><Month>10</Month><Day>21</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-538X</ISSN><JournalIssue CitedMedium="Print"><Volume>79</Volume><Issue>19</Issue><PubDate><Year>2005</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Journal of virology</Title><ISOAbbreviation>J. Virol.</ISOAbbreviation></Journal><ArticleTitle>Minimal T-cell-stimulatory sequences and spectrum of HLA restriction of immunodominant CD4+ T-cell epitopes within hepatitis C virus NS3 and NS4 proteins.</ArticleTitle><Pagination><MedlinePgn>12425-33</MedlinePgn></Pagination><Abstract><AbstractText>The hepatitis C virus (HCV)-specific CD4+ T-cell response against nonstructural proteins is strongly associated with successful viral clearance during acute hepatitis C. To further develop these observations into peptide-based vaccines and clinical immunomonitoring tools like HLA class II tetramers, a detailed characterization of immunodominant CD4+ T-cell epitopes is required. We studied peripheral blood mononuclear cells from 20 patients with acute hepatitis C using 83 overlapping 20-mer peptides covering the NS3 helicase and NS4. Eight peptides were recognized by > or = 40% of patients, and specific CD4+ T-cell clones were obtained for seven of these and three additional, subdominant epitopes. Mapping of minimal stimulatory sequences defined epitopes of 8 to 13 amino acids in length, but optimal T-cell stimulation was observed with 10- to 15-mers. While some epitopes were presented by different HLA molecules, others were presented by only a single HLA class II molecule, which has implications for patient selection in clinical trials of peptide-based immunotherapies. In conclusion, using two different approaches we identified and characterized a set of CD4+ T-cell epitopes in the HCV NS3-NS4 region which are immunodominant in patients achieving transient or persistent viral control. This information allows the construction of a valuable panel of HCV-specific HLA class II tetramers for further study of CD4+ T-cell responses in chronic hepatitis C. The finding of immunodominant epitopes with very constrained HLA restriction has implications for patient selection in clinical trials of peptide-based immunotherapies.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gerlach</LastName><ForeName>J T</ForeName><Initials>JT</Initials><AffiliationInfo><Affiliation>Department of Gastroenterology and Hepatology, University Hospital Zurich, Rämistrasse 100, 8091 Zurich, Switzerland. Tilman.Gerlach@usz.ch</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ulsenheimer</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Grüner</LastName><ForeName>N H</ForeName><Initials>NH</Initials></Author><Author ValidYN="Y"><LastName>Jung</LastName><ForeName>M-C</ForeName><Initials>MC</Initials></Author><Author ValidYN="Y"><LastName>Schraut</LastName><ForeName>W</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Schirren</LastName><ForeName>C-A</ForeName><Initials>CA</Initials></Author><Author ValidYN="Y"><LastName>Heeg</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Scholz</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Witter</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Zahn</LastName><ForeName>R</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Vogler</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Zachoval</LastName><ForeName>R</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Pape</LastName><ForeName>G R</ForeName><Initials>GR</Initials></Author><Author ValidYN="Y"><LastName>Diepolder</LastName><ForeName>H M</ForeName><Initials>HM</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Virol</MedlineTA><NlmUniqueID>0113724</NlmUniqueID><ISSNLinking>0022-538X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006680">HLA Antigens</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016056">Immunodominant Epitopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C084422">NS3 protein, hepatitis C virus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C093542">NS4 protein, hepatitis C virus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017361">Viral Nonstructural Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000293" MajorTopicYN="N">Adolescent</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000483" MajorTopicYN="N">Alleles</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017951" MajorTopicYN="N">Antigen Presentation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015496" MajorTopicYN="N">CD4-Positive T-Lymphocytes</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006680" MajorTopicYN="N">HLA Antigens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016174" MajorTopicYN="N">Hepacivirus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006526" MajorTopicYN="N">Hepatitis C</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016056" MajorTopicYN="Y">Immunodominant Epitopes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007963" MajorTopicYN="N">Leukocytes, Mononuclear</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008213" MajorTopicYN="N">Lymphocyte Activation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017361" MajorTopicYN="N">Viral Nonstructural Proteins</DescriptorName><QualifierName UI="Q000276" 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IdType="pubmed">10622567</ArticleId></ArticleIdList></Reference><Reference><Citation>Gastroenterology. 1999 Oct;117(4):933-41</Citation><ArticleIdList><ArticleId IdType="pubmed">10500077</ArticleId></ArticleIdList></Reference><Reference><Citation>Semin Liver Dis. 2000;20(1):103-26</Citation><ArticleIdList><ArticleId IdType="pubmed">10895435</ArticleId></ArticleIdList></Reference><Reference><Citation>Eur J Immunol. 2001 May;31(5):1438-46</Citation><ArticleIdList><ArticleId IdType="pubmed">11465100</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Med. 2001 Nov 19;194(10):1395-406</Citation><ArticleIdList><ArticleId IdType="pubmed">11714747</ArticleId></ArticleIdList></Reference><Reference><Citation>Hepatology. 2002 May;35(5):1225-36</Citation><ArticleIdList><ArticleId IdType="pubmed">11981773</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2002 Dec;76(24):12584-95</Citation><ArticleIdList><ArticleId IdType="pubmed">12438584</ArticleId></ArticleIdList></Reference><Reference><Citation>Hepatology. 2003 Mar;37(3):577-89</Citation><ArticleIdList><ArticleId IdType="pubmed">12601356</ArticleId></ArticleIdList></Reference><Reference><Citation>Immunogenetics. 2004 Mar;55(12):797-810</Citation><ArticleIdList><ArticleId IdType="pubmed">14963618</ArticleId></ArticleIdList></Reference><Reference><Citation>J Viral Hepat. 2004 Jul;11(4):324-31</Citation><ArticleIdList><ArticleId IdType="pubmed">15230855</ArticleId></ArticleIdList></Reference><Reference><Citation>Hepatology. 2004 Jul;40(1):87-97</Citation><ArticleIdList><ArticleId IdType="pubmed">15239090</ArticleId></ArticleIdList></Reference><Reference><Citation>Hepatology. 1995 Mar;21(3):632-8</Citation><ArticleIdList><ArticleId IdType="pubmed">7875660</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 1995 Oct 14;346(8981):1006-7</Citation><ArticleIdList><ArticleId IdType="pubmed">7475549</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Invest. 1996 Aug 1;98(3):706-14</Citation><ArticleIdList><ArticleId IdType="pubmed">8698862</ArticleId></ArticleIdList></Reference><Reference><Citation>Hepatology. 1997 Feb;25(2):449-58</Citation><ArticleIdList><ArticleId IdType="pubmed">9021963</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1997 Aug;71(8):6011-9</Citation><ArticleIdList><ArticleId IdType="pubmed">9223492</ArticleId></ArticleIdList></Reference><Reference><Citation>Int Immunol. 1999 Apr;11(4):577-83</Citation><ArticleIdList><ArticleId IdType="pubmed">10323211</ArticleId></ArticleIdList></Reference><Reference><Citation>Hepatology. 1999 Oct;30(4):1088-98</Citation><ArticleIdList><ArticleId IdType="pubmed">10498664</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Med. 2000 May 1;191(9):1499-512</Citation><ArticleIdList><ArticleId 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