Synergistic effects of multiple treatments, and both DNA and RNA direct bindings on, green tea catechins.
Identifieur interne : 002189 ( PubMed/Corpus ); précédent : 002188; suivant : 002190Synergistic effects of multiple treatments, and both DNA and RNA direct bindings on, green tea catechins.
Auteurs : Takashi Kuzuhara ; Akitoshi Tanabe ; Yoshihisa Sei ; Kentaro Yamaguchi ; Masami Suganuma ; Hirota FujikiSource :
- Molecular carcinogenesis [ 0899-1987 ] ; 2007.
English descriptors
- KwdEn :
- Apoptosis (drug effects), Camellia sinensis, Catechin (metabolism), Catechin (pharmacology), Cyclin-Dependent Kinase Inhibitor p21 (genetics), DNA, Neoplasm (metabolism), Drug Synergism, Gene Expression Regulation, Neoplastic, Humans, Lung Neoplasms (drug therapy), Lung Neoplasms (genetics), RNA, Neoplasm (metabolism), Tea, Transcription Factor CHOP (genetics), Tumor Cells, Cultured (drug effects), Tumor Cells, Cultured (metabolism).
- MESH :
- chemical , genetics : Cyclin-Dependent Kinase Inhibitor p21, Transcription Factor CHOP.
- chemical , metabolism : Catechin, DNA, Neoplasm, RNA, Neoplasm.
- drug effects : Apoptosis, Tumor Cells, Cultured.
- drug therapy : Lung Neoplasms.
- genetics : Lung Neoplasms.
- metabolism : Tumor Cells, Cultured.
- chemical , pharmacology : Catechin.
- Camellia sinensis, Drug Synergism, Gene Expression Regulation, Neoplastic, Humans, Tea.
Abstract
This article reviews two main topics: (1) the synergistic effects of multiple treatments with green tea catechin and (2) the direct binding of (-)-epigallocatechin gallate (EGCG) to both DNA and RNA molecules. Japanese drink green tea throughout the day, so we studied whether multiple treatments of cells with EGCG would enhance the expression of apoptosis-related genes, such as growth arrest and DNA damage-inducible gene (GADD153) and cyclin-dependent kinase inhibitor gene (p21(waf1)): The results suggest that the synergistic enhancement of both GADD153 and p21(waf1) gene expressions by multiple treatments plays a significant role in human cancer prevention with green tea beverage. Our previous observation-that nucleic acids extracted from catechin-treated cells are colored-allowed us to speculate that catechins directly interact with nucleic acids. Surface plasmon resonance assay (Biacore) indicated that four catechins, EGCG, (-)-epicatechin gallate (ECG), (+)-gallocatechin gallate (GCG), and (+)-catechin gallate (CG), bound to DNA oligomers. Cold spray ionization mass spectrometry (CSI-MS) analysis showed that one to three EGCG molecules bound to single-stranded 18 mers of DNA and RNA. Moreover, one or two molecules of EGCG bound to double-stranded AG:CT oligomers of various nucleotide lengths. Double-stranded DNA (dsDNA) oligomers were detected only as EGCG-bound forms at high temperature, whereas at low temperature both the free and bound forms were detected, suggesting that EGCG protects double-stranded DNA oligomers from double-stranded melting into single-stranded DNA. We assume that catechins accumulate in both double-stranded DNA and RNA molecules through multiple administrations of green tea beverage in in vivo, and that the accumulated green tea catechins play a significant role for human cancer prevention.
DOI: 10.1002/mc.20332
PubMed: 17440927
Links to Exploration step
pubmed:17440927Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Synergistic effects of multiple treatments, and both DNA and RNA direct bindings on, green tea catechins.</title><author><name sortKey="Kuzuhara, Takashi" sort="Kuzuhara, Takashi" uniqKey="Kuzuhara T" first="Takashi" last="Kuzuhara">Takashi Kuzuhara</name><affiliation><nlm:affiliation>Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Tanabe, Akitoshi" sort="Tanabe, Akitoshi" uniqKey="Tanabe A" first="Akitoshi" last="Tanabe">Akitoshi Tanabe</name></author><author><name sortKey="Sei, Yoshihisa" sort="Sei, Yoshihisa" uniqKey="Sei Y" first="Yoshihisa" last="Sei">Yoshihisa Sei</name></author><author><name sortKey="Yamaguchi, Kentaro" sort="Yamaguchi, Kentaro" uniqKey="Yamaguchi K" first="Kentaro" last="Yamaguchi">Kentaro Yamaguchi</name></author><author><name sortKey="Suganuma, Masami" sort="Suganuma, Masami" uniqKey="Suganuma M" first="Masami" last="Suganuma">Masami Suganuma</name></author><author><name sortKey="Fujiki, Hirota" sort="Fujiki, Hirota" uniqKey="Fujiki H" first="Hirota" last="Fujiki">Hirota Fujiki</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17440927</idno><idno type="pmid">17440927</idno><idno type="doi">10.1002/mc.20332</idno><idno type="wicri:Area/PubMed/Corpus">002189</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002189</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Synergistic effects of multiple treatments, and both DNA and RNA direct bindings on, green tea catechins.</title><author><name sortKey="Kuzuhara, Takashi" sort="Kuzuhara, Takashi" uniqKey="Kuzuhara T" first="Takashi" last="Kuzuhara">Takashi Kuzuhara</name><affiliation><nlm:affiliation>Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Tanabe, Akitoshi" sort="Tanabe, Akitoshi" uniqKey="Tanabe A" first="Akitoshi" last="Tanabe">Akitoshi Tanabe</name></author><author><name sortKey="Sei, Yoshihisa" sort="Sei, Yoshihisa" uniqKey="Sei Y" first="Yoshihisa" last="Sei">Yoshihisa Sei</name></author><author><name sortKey="Yamaguchi, Kentaro" sort="Yamaguchi, Kentaro" uniqKey="Yamaguchi K" first="Kentaro" last="Yamaguchi">Kentaro Yamaguchi</name></author><author><name sortKey="Suganuma, Masami" sort="Suganuma, Masami" uniqKey="Suganuma M" first="Masami" last="Suganuma">Masami Suganuma</name></author><author><name sortKey="Fujiki, Hirota" sort="Fujiki, Hirota" uniqKey="Fujiki H" first="Hirota" last="Fujiki">Hirota Fujiki</name></author></analytic><series><title level="j">Molecular carcinogenesis</title><idno type="ISSN">0899-1987</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Apoptosis (drug effects)</term><term>Camellia sinensis</term><term>Catechin (metabolism)</term><term>Catechin (pharmacology)</term><term>Cyclin-Dependent Kinase Inhibitor p21 (genetics)</term><term>DNA, Neoplasm (metabolism)</term><term>Drug Synergism</term><term>Gene Expression Regulation, Neoplastic</term><term>Humans</term><term>Lung Neoplasms (drug therapy)</term><term>Lung Neoplasms (genetics)</term><term>RNA, Neoplasm (metabolism)</term><term>Tea</term><term>Transcription Factor CHOP (genetics)</term><term>Tumor Cells, Cultured (drug effects)</term><term>Tumor Cells, Cultured (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cyclin-Dependent Kinase Inhibitor p21</term><term>Transcription Factor CHOP</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Catechin</term><term>DNA, Neoplasm</term><term>RNA, Neoplasm</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Apoptosis</term><term>Tumor Cells, Cultured</term></keywords><keywords scheme="MESH" qualifier="drug therapy" xml:lang="en"><term>Lung Neoplasms</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Lung Neoplasms</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Tumor Cells, Cultured</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Catechin</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Camellia sinensis</term><term>Drug Synergism</term><term>Gene Expression Regulation, Neoplastic</term><term>Humans</term><term>Tea</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This article reviews two main topics: (1) the synergistic effects of multiple treatments with green tea catechin and (2) the direct binding of (-)-epigallocatechin gallate (EGCG) to both DNA and RNA molecules. Japanese drink green tea throughout the day, so we studied whether multiple treatments of cells with EGCG would enhance the expression of apoptosis-related genes, such as growth arrest and DNA damage-inducible gene (GADD153) and cyclin-dependent kinase inhibitor gene (p21(waf1)): The results suggest that the synergistic enhancement of both GADD153 and p21(waf1) gene expressions by multiple treatments plays a significant role in human cancer prevention with green tea beverage. Our previous observation-that nucleic acids extracted from catechin-treated cells are colored-allowed us to speculate that catechins directly interact with nucleic acids. Surface plasmon resonance assay (Biacore) indicated that four catechins, EGCG, (-)-epicatechin gallate (ECG), (+)-gallocatechin gallate (GCG), and (+)-catechin gallate (CG), bound to DNA oligomers. Cold spray ionization mass spectrometry (CSI-MS) analysis showed that one to three EGCG molecules bound to single-stranded 18 mers of DNA and RNA. Moreover, one or two molecules of EGCG bound to double-stranded AG:CT oligomers of various nucleotide lengths. Double-stranded DNA (dsDNA) oligomers were detected only as EGCG-bound forms at high temperature, whereas at low temperature both the free and bound forms were detected, suggesting that EGCG protects double-stranded DNA oligomers from double-stranded melting into single-stranded DNA. We assume that catechins accumulate in both double-stranded DNA and RNA molecules through multiple administrations of green tea beverage in in vivo, and that the accumulated green tea catechins play a significant role for human cancer prevention.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17440927</PMID><DateCompleted><Year>2007</Year><Month>09</Month><Day>18</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0899-1987</ISSN><JournalIssue CitedMedium="Print"><Volume>46</Volume><Issue>8</Issue><PubDate><Year>2007</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Molecular carcinogenesis</Title><ISOAbbreviation>Mol. Carcinog.</ISOAbbreviation></Journal><ArticleTitle>Synergistic effects of multiple treatments, and both DNA and RNA direct bindings on, green tea catechins.</ArticleTitle><Pagination><MedlinePgn>640-5</MedlinePgn></Pagination><Abstract><AbstractText>This article reviews two main topics: (1) the synergistic effects of multiple treatments with green tea catechin and (2) the direct binding of (-)-epigallocatechin gallate (EGCG) to both DNA and RNA molecules. Japanese drink green tea throughout the day, so we studied whether multiple treatments of cells with EGCG would enhance the expression of apoptosis-related genes, such as growth arrest and DNA damage-inducible gene (GADD153) and cyclin-dependent kinase inhibitor gene (p21(waf1)): The results suggest that the synergistic enhancement of both GADD153 and p21(waf1) gene expressions by multiple treatments plays a significant role in human cancer prevention with green tea beverage. Our previous observation-that nucleic acids extracted from catechin-treated cells are colored-allowed us to speculate that catechins directly interact with nucleic acids. Surface plasmon resonance assay (Biacore) indicated that four catechins, EGCG, (-)-epicatechin gallate (ECG), (+)-gallocatechin gallate (GCG), and (+)-catechin gallate (CG), bound to DNA oligomers. Cold spray ionization mass spectrometry (CSI-MS) analysis showed that one to three EGCG molecules bound to single-stranded 18 mers of DNA and RNA. Moreover, one or two molecules of EGCG bound to double-stranded AG:CT oligomers of various nucleotide lengths. Double-stranded DNA (dsDNA) oligomers were detected only as EGCG-bound forms at high temperature, whereas at low temperature both the free and bound forms were detected, suggesting that EGCG protects double-stranded DNA oligomers from double-stranded melting into single-stranded DNA. We assume that catechins accumulate in both double-stranded DNA and RNA molecules through multiple administrations of green tea beverage in in vivo, and that the accumulated green tea catechins play a significant role for human cancer prevention.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kuzuhara</LastName><ForeName>Takashi</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tanabe</LastName><ForeName>Akitoshi</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Sei</LastName><ForeName>Yoshihisa</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Yamaguchi</LastName><ForeName>Kentaro</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Suganuma</LastName><ForeName>Masami</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Fujiki</LastName><ForeName>Hirota</ForeName><Initials>H</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Carcinog</MedlineTA><NlmUniqueID>8811105</NlmUniqueID><ISSNLinking>0899-1987</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C493956">CDKN1A protein, human</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D050759">Cyclin-Dependent Kinase Inhibitor p21</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C497249">DDIT3 protein, human</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004273">DNA, Neoplasm</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012334">RNA, Neoplasm</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013662">Tea</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-12-7</RegistryNumber><NameOfSubstance UI="D051742">Transcription Factor CHOP</NameOfSubstance></Chemical><Chemical><RegistryNumber>8R1V1STN48</RegistryNumber><NameOfSubstance UI="D002392">Catechin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017209" MajorTopicYN="N">Apoptosis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D028241" MajorTopicYN="Y">Camellia sinensis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002392" MajorTopicYN="N">Catechin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D050759" MajorTopicYN="N">Cyclin-Dependent Kinase Inhibitor p21</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004273" MajorTopicYN="N">DNA, Neoplasm</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004357" MajorTopicYN="N">Drug Synergism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015972" MajorTopicYN="N">Gene Expression Regulation, Neoplastic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008175" MajorTopicYN="N">Lung Neoplasms</DescriptorName><QualifierName UI="Q000188" MajorTopicYN="N">drug therapy</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012334" MajorTopicYN="N">RNA, Neoplasm</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013662" MajorTopicYN="N">Tea</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051742" MajorTopicYN="N">Transcription Factor CHOP</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014407" MajorTopicYN="N">Tumor Cells, Cultured</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>4</Month><Day>19</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>9</Month><Day>19</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>4</Month><Day>19</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17440927</ArticleId><ArticleId IdType="doi">10.1002/mc.20332</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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