Amino acid sequence of B-cell epitope of N-terminal region of ESAT-6 Mycobacterium leprae role as specific antigen for diagnosis of leprosy.
Identifieur interne : 001F24 ( PubMed/Corpus ); précédent : 001F23; suivant : 001F25Amino acid sequence of B-cell epitope of N-terminal region of ESAT-6 Mycobacterium leprae role as specific antigen for diagnosis of leprosy.
Auteurs : Fauziah Nuraini Kurdi ; Indro Handojo ; Indropo Agoesni ; Yoes Priatna DahlanSource :
- The Southeast Asian journal of tropical medicine and public health [ 0125-1562 ] ; 2010.
English descriptors
- KwdEn :
- Adolescent, Adult, Amino Acid Sequence, Antigens, Bacterial (chemistry), Antigens, Bacterial (immunology), Bacterial Proteins (chemistry), Bacterial Proteins (immunology), Epitopes, B-Lymphocyte (chemistry), Epitopes, B-Lymphocyte (immunology), Female, Humans, Leprosy, Tuberculoid (diagnosis), Leprosy, Tuberculoid (immunology), Male, Middle Aged, Mycobacterium leprae (chemistry), Mycobacterium leprae (immunology).
- MESH :
- chemical , chemistry : Antigens, Bacterial, Bacterial Proteins, Epitopes, B-Lymphocyte.
- chemical , immunology : Antigens, Bacterial, Bacterial Proteins, Epitopes, B-Lymphocyte.
- chemistry : Mycobacterium leprae.
- diagnosis : Leprosy, Tuberculoid.
- immunology : Leprosy, Tuberculoid, Mycobacterium leprae.
- Adolescent, Adult, Amino Acid Sequence, Female, Humans, Male, Middle Aged.
Abstract
The objective of this study was to find a specific B-cell epitope of N-terminal region of antigen L-ESAT-6 from leprosy patients, healthy individuals and healthy nurses working for more than 10 years in the leprosy ward of Dr. A. Rivai Abdullah Leprosy Hospital, Palembang, Indonesia. Fifty subjects were enrolled in this study, comprising 10 subjects with LL type leprosy, 10 subjects with BB type leprosy, 10 subjects with TT type leprosy, 10 healthy nurses from leprosy ward and 10 healthy individuals as control group. The amino acid sequence of residues 11-36 of the N-terminal region of L-ESAT-6 were divided into a series of 18 peptides each consisting of 9-mer peptides with an overlap of 8-mers and an offset of one amino acid. The series of 18 peptides were synthesized in the form of biotinylated peptides and used to screen sera of 50 subjects using an indirect ELISA method. Our study identified at the N-terminal of L-ESAT-6, LEQCQES, VNELQG and IDALLE as epitope marker for LL and BB type of leprosy, epitope marker for TT type of leprosy and for (protective epitope marker) healthy nurses working for more than 10 years in the leprosy ward, respectively. These antigens can be used in immunochromatographic test for the early diagnosis of leprosy.
PubMed: 21073037
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Amino acid sequence of B-cell epitope of N-terminal region of ESAT-6 Mycobacterium leprae role as specific antigen for diagnosis of leprosy.</title><author><name sortKey="Kurdi, Fauziah Nuraini" sort="Kurdi, Fauziah Nuraini" uniqKey="Kurdi F" first="Fauziah Nuraini" last="Kurdi">Fauziah Nuraini Kurdi</name><affiliation><nlm:affiliation>Physical and Health Study Program, Teacher Training and Education Faculty, Sriwijaya University, Palembang, Indonesia. fnkurdi@yahoo.com</nlm:affiliation></affiliation></author><author><name sortKey="Handojo, Indro" sort="Handojo, Indro" uniqKey="Handojo I" first="Indro" last="Handojo">Indro Handojo</name></author><author><name sortKey="Agoesni, Indropo" sort="Agoesni, Indropo" uniqKey="Agoesni I" first="Indropo" last="Agoesni">Indropo Agoesni</name></author><author><name sortKey="Dahlan, Yoes Priatna" sort="Dahlan, Yoes Priatna" uniqKey="Dahlan Y" first="Yoes Priatna" last="Dahlan">Yoes Priatna Dahlan</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:21073037</idno><idno type="pmid">21073037</idno><idno type="wicri:Area/PubMed/Corpus">001F24</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001F24</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Amino acid sequence of B-cell epitope of N-terminal region of ESAT-6 Mycobacterium leprae role as specific antigen for diagnosis of leprosy.</title><author><name sortKey="Kurdi, Fauziah Nuraini" sort="Kurdi, Fauziah Nuraini" uniqKey="Kurdi F" first="Fauziah Nuraini" last="Kurdi">Fauziah Nuraini Kurdi</name><affiliation><nlm:affiliation>Physical and Health Study Program, Teacher Training and Education Faculty, Sriwijaya University, Palembang, Indonesia. fnkurdi@yahoo.com</nlm:affiliation></affiliation></author><author><name sortKey="Handojo, Indro" sort="Handojo, Indro" uniqKey="Handojo I" first="Indro" last="Handojo">Indro Handojo</name></author><author><name sortKey="Agoesni, Indropo" sort="Agoesni, Indropo" uniqKey="Agoesni I" first="Indropo" last="Agoesni">Indropo Agoesni</name></author><author><name sortKey="Dahlan, Yoes Priatna" sort="Dahlan, Yoes Priatna" uniqKey="Dahlan Y" first="Yoes Priatna" last="Dahlan">Yoes Priatna Dahlan</name></author></analytic><series><title level="j">The Southeast Asian journal of tropical medicine and public health</title><idno type="ISSN">0125-1562</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Amino Acid Sequence</term><term>Antigens, Bacterial (chemistry)</term><term>Antigens, Bacterial (immunology)</term><term>Bacterial Proteins (chemistry)</term><term>Bacterial Proteins (immunology)</term><term>Epitopes, B-Lymphocyte (chemistry)</term><term>Epitopes, B-Lymphocyte (immunology)</term><term>Female</term><term>Humans</term><term>Leprosy, Tuberculoid (diagnosis)</term><term>Leprosy, Tuberculoid (immunology)</term><term>Male</term><term>Middle Aged</term><term>Mycobacterium leprae (chemistry)</term><term>Mycobacterium leprae (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antigens, Bacterial</term><term>Bacterial Proteins</term><term>Epitopes, B-Lymphocyte</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Bacterial</term><term>Bacterial Proteins</term><term>Epitopes, B-Lymphocyte</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Mycobacterium leprae</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Leprosy, Tuberculoid</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Leprosy, Tuberculoid</term><term>Mycobacterium leprae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Amino Acid Sequence</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The objective of this study was to find a specific B-cell epitope of N-terminal region of antigen L-ESAT-6 from leprosy patients, healthy individuals and healthy nurses working for more than 10 years in the leprosy ward of Dr. A. Rivai Abdullah Leprosy Hospital, Palembang, Indonesia. Fifty subjects were enrolled in this study, comprising 10 subjects with LL type leprosy, 10 subjects with BB type leprosy, 10 subjects with TT type leprosy, 10 healthy nurses from leprosy ward and 10 healthy individuals as control group. The amino acid sequence of residues 11-36 of the N-terminal region of L-ESAT-6 were divided into a series of 18 peptides each consisting of 9-mer peptides with an overlap of 8-mers and an offset of one amino acid. The series of 18 peptides were synthesized in the form of biotinylated peptides and used to screen sera of 50 subjects using an indirect ELISA method. Our study identified at the N-terminal of L-ESAT-6, LEQCQES, VNELQG and IDALLE as epitope marker for LL and BB type of leprosy, epitope marker for TT type of leprosy and for (protective epitope marker) healthy nurses working for more than 10 years in the leprosy ward, respectively. These antigens can be used in immunochromatographic test for the early diagnosis of leprosy.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21073037</PMID><DateCompleted><Year>2010</Year><Month>12</Month><Day>23</Day></DateCompleted><DateRevised><Year>2010</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0125-1562</ISSN><JournalIssue CitedMedium="Print"><Volume>41</Volume><Issue>5</Issue><PubDate><Year>2010</Year><Month>Sep</Month></PubDate></JournalIssue><Title>The Southeast Asian journal of tropical medicine and public health</Title><ISOAbbreviation>Southeast Asian J. Trop. Med. Public Health</ISOAbbreviation></Journal><ArticleTitle>Amino acid sequence of B-cell epitope of N-terminal region of ESAT-6 Mycobacterium leprae role as specific antigen for diagnosis of leprosy.</ArticleTitle><Pagination><MedlinePgn>1158-63</MedlinePgn></Pagination><Abstract><AbstractText>The objective of this study was to find a specific B-cell epitope of N-terminal region of antigen L-ESAT-6 from leprosy patients, healthy individuals and healthy nurses working for more than 10 years in the leprosy ward of Dr. A. Rivai Abdullah Leprosy Hospital, Palembang, Indonesia. Fifty subjects were enrolled in this study, comprising 10 subjects with LL type leprosy, 10 subjects with BB type leprosy, 10 subjects with TT type leprosy, 10 healthy nurses from leprosy ward and 10 healthy individuals as control group. The amino acid sequence of residues 11-36 of the N-terminal region of L-ESAT-6 were divided into a series of 18 peptides each consisting of 9-mer peptides with an overlap of 8-mers and an offset of one amino acid. The series of 18 peptides were synthesized in the form of biotinylated peptides and used to screen sera of 50 subjects using an indirect ELISA method. Our study identified at the N-terminal of L-ESAT-6, LEQCQES, VNELQG and IDALLE as epitope marker for LL and BB type of leprosy, epitope marker for TT type of leprosy and for (protective epitope marker) healthy nurses working for more than 10 years in the leprosy ward, respectively. These antigens can be used in immunochromatographic test for the early diagnosis of leprosy.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kurdi</LastName><ForeName>Fauziah Nuraini</ForeName><Initials>FN</Initials><AffiliationInfo><Affiliation>Physical and Health Study Program, Teacher Training and Education Faculty, Sriwijaya University, Palembang, Indonesia. fnkurdi@yahoo.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Handojo</LastName><ForeName>Indro</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Agoesni</LastName><ForeName>Indropo</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Dahlan</LastName><ForeName>Yoes Priatna</ForeName><Initials>YP</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Thailand</Country><MedlineTA>Southeast Asian J Trop Med Public Health</MedlineTA><NlmUniqueID>0266303</NlmUniqueID><ISSNLinking>0125-1562</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000942">Antigens, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C525005">ESAT-6 antigen, Mycobacterium leprae</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018985">Epitopes, B-Lymphocyte</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000293" MajorTopicYN="N">Adolescent</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000942" MajorTopicYN="N">Antigens, Bacterial</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018985" MajorTopicYN="N">Epitopes, B-Lymphocyte</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015441" MajorTopicYN="N">Leprosy, Tuberculoid</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009166" MajorTopicYN="N">Mycobacterium leprae</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>11</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>11</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>12</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21073037</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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