Bat origins of MERS-CoV supported by bat coronavirus HKU4 usage of human receptor CD26.
Identifieur interne : 001850 ( PubMed/Corpus ); précédent : 001849; suivant : 001851Bat origins of MERS-CoV supported by bat coronavirus HKU4 usage of human receptor CD26.
Auteurs : Qihui Wang ; Jianxun Qi ; Yuan Yuan ; Yifang Xuan ; Pengcheng Han ; Yuhua Wan ; Wei Ji ; Yan Li ; Ying Wu ; Jianwei Wang ; Aikichi Iwamoto ; Patrick C Y. Woo ; Kwok-Yung Yuen ; Jinghua Yan ; Guangwen Lu ; George F. GaoSource :
- Cell host & microbe [ 1934-6069 ] ; 2014.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Chiroptera (virology), Coronavirus (classification), Coronavirus (genetics), Coronavirus (isolation & purification), Coronavirus (metabolism), Coronavirus Infections (genetics), Coronavirus Infections (metabolism), Coronavirus Infections (virology), Dipeptidyl Peptidase 4 (genetics), Dipeptidyl Peptidase 4 (metabolism), Humans, Middle East Respiratory Syndrome Coronavirus (classification), Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (metabolism), Molecular Sequence Data, Phylogeny, Protein Binding, Receptors, Virus (chemistry), Receptors, Virus (genetics), Receptors, Virus (metabolism), Sequence Alignment, Spike Glycoprotein, Coronavirus (chemistry), Spike Glycoprotein, Coronavirus (genetics), Spike Glycoprotein, Coronavirus (metabolism).
- MESH :
- chemical , chemistry : Receptors, Virus, Spike Glycoprotein, Coronavirus.
- chemical , genetics : Dipeptidyl Peptidase 4, Receptors, Virus, Spike Glycoprotein, Coronavirus.
- classification : Coronavirus, Middle East Respiratory Syndrome Coronavirus.
- genetics : Coronavirus, Coronavirus Infections, Middle East Respiratory Syndrome Coronavirus.
- isolation & purification : Coronavirus.
- metabolism : Coronavirus, Coronavirus Infections, Dipeptidyl Peptidase 4, Middle East Respiratory Syndrome Coronavirus, Receptors, Virus, Spike Glycoprotein, Coronavirus.
- virology : Chiroptera, Coronavirus Infections.
- Amino Acid Sequence, Animals, Humans, Molecular Sequence Data, Phylogeny, Protein Binding, Sequence Alignment.
Abstract
The recently reported Middle East respiratory syndrome coronavirus (MERS-CoV) is phylogenetically closely related to the bat coronaviruses (BatCoVs) HKU4 and HKU5. However, the evolutionary pathway of MERS-CoV is still unclear. A receptor binding domain (RBD) in the MERS-CoV envelope-embedded spike protein specifically engages human CD26 (hCD26) to initiate viral entry. The high sequence identity in the viral spike protein prompted us to investigate if HKU4 and HKU5 can recognize hCD26 for cell entry. We found that HKU4-RBD, but not HKU5-RBD, binds to hCD26, and pseudotyped viruses embedding HKU4 spike can infect cells via hCD26 recognition. The structure of the HKU4-RBD/hCD26 complex revealed a hCD26-binding mode similar overall to that observed for MERS-RBD. HKU4-RBD, however, is less adapted to hCD26 than MERS-RBD, explaining its lower affinity for receptor binding. Our findings support a bat origin for MERS-CoV and indicate the need for surveillance of HKU4-related viruses in bats.
DOI: 10.1016/j.chom.2014.08.009
PubMed: 25211075
Links to Exploration step
pubmed:25211075Le document en format XML
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<term>Coronavirus (genetics)</term>
<term>Coronavirus (isolation & purification)</term>
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<front><div type="abstract" xml:lang="en">The recently reported Middle East respiratory syndrome coronavirus (MERS-CoV) is phylogenetically closely related to the bat coronaviruses (BatCoVs) HKU4 and HKU5. However, the evolutionary pathway of MERS-CoV is still unclear. A receptor binding domain (RBD) in the MERS-CoV envelope-embedded spike protein specifically engages human CD26 (hCD26) to initiate viral entry. The high sequence identity in the viral spike protein prompted us to investigate if HKU4 and HKU5 can recognize hCD26 for cell entry. We found that HKU4-RBD, but not HKU5-RBD, binds to hCD26, and pseudotyped viruses embedding HKU4 spike can infect cells via hCD26 recognition. The structure of the HKU4-RBD/hCD26 complex revealed a hCD26-binding mode similar overall to that observed for MERS-RBD. HKU4-RBD, however, is less adapted to hCD26 than MERS-RBD, explaining its lower affinity for receptor binding. Our findings support a bat origin for MERS-CoV and indicate the need for surveillance of HKU4-related viruses in bats. </div>
</front>
</TEI>
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<DateCompleted><Year>2015</Year>
<Month>06</Month>
<Day>16</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>04</Month>
<Day>01</Day>
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<Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1934-6069</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>16</Volume>
<Issue>3</Issue>
<PubDate><Year>2014</Year>
<Month>Sep</Month>
<Day>10</Day>
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<Title>Cell host & microbe</Title>
<ISOAbbreviation>Cell Host Microbe</ISOAbbreviation>
</Journal>
<ArticleTitle>Bat origins of MERS-CoV supported by bat coronavirus HKU4 usage of human receptor CD26.</ArticleTitle>
<Pagination><MedlinePgn>328-37</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1016/j.chom.2014.08.009</ELocationID>
<ELocationID EIdType="pii" ValidYN="Y">S1931-3128(14)00301-1</ELocationID>
<Abstract><AbstractText>The recently reported Middle East respiratory syndrome coronavirus (MERS-CoV) is phylogenetically closely related to the bat coronaviruses (BatCoVs) HKU4 and HKU5. However, the evolutionary pathway of MERS-CoV is still unclear. A receptor binding domain (RBD) in the MERS-CoV envelope-embedded spike protein specifically engages human CD26 (hCD26) to initiate viral entry. The high sequence identity in the viral spike protein prompted us to investigate if HKU4 and HKU5 can recognize hCD26 for cell entry. We found that HKU4-RBD, but not HKU5-RBD, binds to hCD26, and pseudotyped viruses embedding HKU4 spike can infect cells via hCD26 recognition. The structure of the HKU4-RBD/hCD26 complex revealed a hCD26-binding mode similar overall to that observed for MERS-RBD. HKU4-RBD, however, is less adapted to hCD26 than MERS-RBD, explaining its lower affinity for receptor binding. Our findings support a bat origin for MERS-CoV and indicate the need for surveillance of HKU4-related viruses in bats. </AbstractText>
<CopyrightInformation>Copyright © 2014 Elsevier Inc. All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Qihui</ForeName>
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</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Qi</LastName>
<ForeName>Jianxun</ForeName>
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</AffiliationInfo>
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<Initials>Y</Initials>
<AffiliationInfo><Affiliation>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; School of Life Sciences, University of Science and Technology of China, Hefei 230027, Anhui Province, China.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Xuan</LastName>
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<Initials>Y</Initials>
<AffiliationInfo><Affiliation>Research Network of Immunity and Health, Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Han</LastName>
<ForeName>Pengcheng</ForeName>
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</AffiliationInfo>
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</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ji</LastName>
<ForeName>Wei</ForeName>
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<Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>Yan</ForeName>
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<ForeName>Ying</ForeName>
<Initials>Y</Initials>
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</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Jianwei</ForeName>
<Initials>J</Initials>
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</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Iwamoto</LastName>
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<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year>
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<ArticleIdList><ArticleId IdType="pubmed">25211075</ArticleId>
<ArticleId IdType="pii">S1931-3128(14)00301-1</ArticleId>
<ArticleId IdType="doi">10.1016/j.chom.2014.08.009</ArticleId>
<ArticleId IdType="pmc">PMC7104937</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2009 Nov 24;106(47):19970-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19901337</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nature. 2013 Aug 8;500(7461):227-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23831647</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 2006 Jul 20;351(1):180-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16647731</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Clin Lab Med. 2009 Dec;29(4):715-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19892230</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2011 Jun 28;108(26):10696-701</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21670291</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Mol Biol Evol. 2013 Dec;30(12):2725-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24132122</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>BMC Infect Dis. 2012 Dec 20;12:365</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23256846</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2009 Jan;83(2):908-17</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18971277</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Pediatr Infect Dis J. 2013 Apr;32(4):325-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23337903</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Clin Infect Dis. 2005 Jun 15;40(12):1721-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15909257</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2013 Dec;87(24):13134-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24067982</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Lancet Infect Dis. 2013 Oct;13(10):859-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23933067</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2004 Oct;78(19):10628-35</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15367630</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1953-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12690092</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Scand J Immunol. 2001 Sep;54(3):249-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11555388</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nature. 1992 Jun 4;357(6377):417-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1350661</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Antiviral Res. 2013 Dec;100(3):605-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24121034</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Biol Chem. 2004 Jul 16;279(29):30514-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15123674</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Science. 2003 Oct 10;302(5643):276-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12958366</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2012 Apr;86(7):3995-4008</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22278237</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Lancet Infect Dis. 2014 Feb;14(2):140-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24355866</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>N Engl J Med. 2012 Nov 8;367(19):1814-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23075143</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nature. 2003 Nov 27;426(6965):450-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14647384</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nature. 2013 Nov 28;503(7477):535-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24172901</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Infect Dis. 2009 Mar 15;199(6):847-57</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19239338</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Bioinformatics. 1999 Apr;15(4):305-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10320398</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2005 May 31;102(22):7988-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15897467</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2014 Aug 26;111(34):12516-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25114257</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5063-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12690074</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nature. 2013 Mar 14;495(7440):251-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23486063</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2007 Feb;81(4):1574-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17121802</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Emerg Infect Dis. 2013 Nov;19(11):1819-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24206838</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Emerg Infect Dis. 2013 Oct;19(10):1697-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24050621</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Biol Chem. 2004 Nov 19;279(47):49414-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15345712</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Mar 23;101(12):4240-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15010527</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nat Struct Mol Biol. 2008 Jul;15(7):690-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18596815</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>mBio. 2012 Nov 20;3(6):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23170002</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2013 Aug;87(15):8638-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23720729</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Euro Surveill. 2012 Oct 04;17(40):20290</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23078800</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Cell Res. 2013 Aug;23(8):986-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23835475</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Science. 2005 Sep 16;309(5742):1864-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16166518</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Biol Chem. 2004 Oct 8;279(41):43330-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15213224</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Protein Cell. 2012 Nov;3(11):803-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23143870</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2008 Feb;82(4):1899-907</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18077725</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1985 Dec;56(3):912-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2999444</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Science. 2005 Oct 28;310(5748):676-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16195424</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS Pathog. 2012;8(8):e1002859</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22876187</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2013 May;87(10):5502-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23468491</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nature. 1992 Jun 4;357(6377):420-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1350662</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
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