MersV1, PubMed, Corpus, bibRecord, 001690

In situ imidazole activation of ribonucleotides for abiotic RNA oligomerization reactions.

Identifieur interne : 001690 ( PubMed/Corpus ); précédent : 001689; suivant : 001691

In situ imidazole activation of ribonucleotides for abiotic RNA oligomerization reactions.

Auteurs : Bradley T. Burcar ; Mohsin Jawed ; Hari Shah ; Linda B. Mcgown

Source :

Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life [ 1573-0875 ] ; 2015.

RBID : pubmed:25716919

English descriptors

KwdEn :
- Bentonite (chemistry), Catalysis, Evolution, Chemical, Imidazoles (chemistry), RNA (chemistry), Ribonucleotides (chemistry).
MESH :
- chemical , chemistry : Bentonite, Imidazoles, RNA, Ribonucleotides.
- Catalysis, Evolution, Chemical.

Abstract

The hypothesis that RNA played a significant role in the origin of life requires effective and efficient abiotic pathways to produce RNA oligomers. The most successful abiotic oligomerization reactions to date have utilized high-energy, modified, or pre-activated ribonucleotides to generate strands of RNA up to 50-mers in length. In spite of their success, these modifications and pre-activation reactions significantly alter the ribonucleotides in ways that are highly unlikely to have occurred on a prebiotic Earth. This research seeks to address this problem by exploring an aqueous based method for activating the canonical ribonucleotides in situ using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and imidazole. The reactions were run with and without a montmorillonite clay catalyst and compared to reactions that used ribonucleotides that were pre-activated with imidazole. The effects of pH and ribonucleotide concentration were also investigated. The results demonstrate the ability of in situ activation of ribonucleotides to generate linear RNA oligomers in solution, providing an alternative route to produce RNA for use in prebiotic Earth scenarios.

DOI: 10.1007/s11084-015-9412-y
PubMed: 25716919

Links to Exploration step

pubmed:25716919

Le document en format XML

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<author><name sortKey="Burcar, Bradley T" sort="Burcar, Bradley T" uniqKey="Burcar B" first="Bradley T" last="Burcar">Bradley T. Burcar</name>
<affiliation><nlm:affiliation>New York Center for Astrobiology and the Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, 110 8th Street Troy, New York, 12180, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Jawed, Mohsin" sort="Jawed, Mohsin" uniqKey="Jawed M" first="Mohsin" last="Jawed">Mohsin Jawed</name>
</author>
<author><name sortKey="Shah, Hari" sort="Shah, Hari" uniqKey="Shah H" first="Hari" last="Shah">Hari Shah</name>
</author>
<author><name sortKey="Mcgown, Linda B" sort="Mcgown, Linda B" uniqKey="Mcgown L" first="Linda B" last="Mcgown">Linda B. Mcgown</name>
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<author><name sortKey="Jawed, Mohsin" sort="Jawed, Mohsin" uniqKey="Jawed M" first="Mohsin" last="Jawed">Mohsin Jawed</name>
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<author><name sortKey="Shah, Hari" sort="Shah, Hari" uniqKey="Shah H" first="Hari" last="Shah">Hari Shah</name>
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<author><name sortKey="Mcgown, Linda B" sort="Mcgown, Linda B" uniqKey="Mcgown L" first="Linda B" last="Mcgown">Linda B. Mcgown</name>
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<term>RNA (chemistry)</term>
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<front><div type="abstract" xml:lang="en">The hypothesis that RNA played a significant role in the origin of life requires effective and efficient abiotic pathways to produce RNA oligomers. The most successful abiotic oligomerization reactions to date have utilized high-energy, modified, or pre-activated ribonucleotides to generate strands of RNA up to 50-mers in length. In spite of their success, these modifications and pre-activation reactions significantly alter the ribonucleotides in ways that are highly unlikely to have occurred on a prebiotic Earth. This research seeks to address this problem by exploring an aqueous based method for activating the canonical ribonucleotides in situ using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and imidazole. The reactions were run with and without a montmorillonite clay catalyst and compared to reactions that used ribonucleotides that were pre-activated with imidazole. The effects of pH and ribonucleotide concentration were also investigated. The results demonstrate the ability of in situ activation of ribonucleotides to generate linear RNA oligomers in solution, providing an alternative route to produce RNA for use in prebiotic Earth scenarios. </div>
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In situ imidazole activation of ribonucleotides for abiotic RNA oligomerization reactions.

In situ imidazole activation of ribonucleotides for abiotic RNA oligomerization reactions.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

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