External Quality Assessment of MERS-CoV Molecular Diagnostics During the 2015 Korean Outbreak.
Identifieur interne : 001243 ( PubMed/Corpus ); précédent : 001242; suivant : 001244External Quality Assessment of MERS-CoV Molecular Diagnostics During the 2015 Korean Outbreak.
Auteurs : Moon Woo Seong ; Seung Jun Lee ; Sung Im Cho ; Kyungphil Ko ; Mi Na Kim ; Heungsub Sung ; Jae Seok Kim ; Ji Soo Ahn ; Byung Su Yu ; Taek Soo Kim ; Eui Chong Kim ; Sung Sup ParkSource :
- Annals of laboratory medicine [ 2234-3814 ] ; 2016.
English descriptors
- KwdEn :
- Coronavirus Infections (diagnosis), Coronavirus Infections (epidemiology), Coronavirus Infections (virology), Disease Outbreaks, Humans, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Molecular Diagnostic Techniques (standards), Quality Assurance, Health Care, RNA, Viral (analysis), Real-Time Polymerase Chain Reaction, Republic of Korea (epidemiology), Surveys and Questionnaires.
- MESH :
- chemical , analysis : RNA, Viral.
- diagnosis : Coronavirus Infections.
- epidemiology : Coronavirus Infections, Republic of Korea.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- standards : Molecular Diagnostic Techniques.
- virology : Coronavirus Infections.
- Disease Outbreaks, Humans, Quality Assurance, Health Care, Real-Time Polymerase Chain Reaction, Surveys and Questionnaires.
Abstract
The largest outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) infection outside Middle East Asia in 2015 has necessitated the rapid expansion of laboratories that conduct MERS-CoV molecular testing in Korea, together with external quality assessment (EQA) to evaluate the assays used.
DOI: 10.3343/alm.2016.36.3.230
PubMed: 26915611
Links to Exploration step
pubmed:26915611Le document en format XML
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<term>Disease Outbreaks</term>
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<term>Middle East Respiratory Syndrome Coronavirus (genetics)</term>
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<front><div type="abstract" xml:lang="en">The largest outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) infection outside Middle East Asia in 2015 has necessitated the rapid expansion of laboratories that conduct MERS-CoV molecular testing in Korea, together with external quality assessment (EQA) to evaluate the assays used.</div>
</front>
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<Month>01</Month>
<Day>09</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
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<JournalIssue CitedMedium="Internet"><Volume>36</Volume>
<Issue>3</Issue>
<PubDate><Year>2016</Year>
<Month>May</Month>
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<Title>Annals of laboratory medicine</Title>
<ISOAbbreviation>Ann Lab Med</ISOAbbreviation>
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<ArticleTitle>External Quality Assessment of MERS-CoV Molecular Diagnostics During the 2015 Korean Outbreak.</ArticleTitle>
<Pagination><MedlinePgn>230-4</MedlinePgn>
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<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">The largest outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) infection outside Middle East Asia in 2015 has necessitated the rapid expansion of laboratories that conduct MERS-CoV molecular testing in Korea, together with external quality assessment (EQA) to evaluate the assays used.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">The EQA program consisted of two phases; self-validation and blind assessment. For the first EQA phase, in vitro transcribed upstream region of the envelope gene (upE) and the open reading frame (ORF)1a RNAs were used at a concentration of 1,000 copies/μL. The test panel for the second EQA phase consisted of RNA extracts from three samples, which were obtained from two MERS-CoV positive patients and one MERS-CoV negative patient.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">The first EQA phase results for 46 participants showed a linear relationship between the threshold cycle (C(T)) values of RNA materials and the logarithmic concentrations for both upE and ORF1a gene targets (R²=0.73 and 0.75, respectively). The mean C(T) value for each concentration was different depending on which commercial kit was used for the assay. Among the three commonly used kits, PowerChek MERS Real-Time PCR kit (KogeneBiotech, Korea) showed the lowest C(T) values at all concentrations of upE and most concentrations of ORF1a. The second EQA phase results for 47 participants were 100% correct for all tested samples.</AbstractText>
<AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">This EQA survey demonstrates that the MERS-CoV molecular testing performed in Korea during the 2015 outbreak is of robust capability. However, careful establishment and validation of a cut-off value are recommended to ensure good analytical sensitivity.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Seong</LastName>
<ForeName>Moon Woo</ForeName>
<Initials>MW</Initials>
<AffiliationInfo><Affiliation>Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.</Affiliation>
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<ForeName>Sung Sup</ForeName>
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<AffiliationInfo><Affiliation>Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea. sparkle@snu.ac.kr.</Affiliation>
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