MersV1, PubMed, Corpus, bibRecord, 001153

MERS-CoV diagnosis: An update.

Identifieur interne : 001153 ( PubMed/Corpus ); précédent : 001152; suivant : 001154

MERS-CoV diagnosis: An update.

Auteurs : Sameera Al Johani ; Ali H. Hajeer

Source :

Journal of infection and public health [ 1876-035X ]

RBID : pubmed:27106390

English descriptors

KwdEn :
- Coronavirus Infections (diagnosis), Humans, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Molecular Diagnostic Techniques (methods), Real-Time Polymerase Chain Reaction (methods), Sequence Analysis, DNA (methods), Serologic Tests (methods).
MESH :
- diagnosis : Coronavirus Infections.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- methods : Molecular Diagnostic Techniques, Real-Time Polymerase Chain Reaction, Sequence Analysis, DNA, Serologic Tests.
- Humans.

Abstract

Diagnosis of MERS-Cov still a major concern in most of daignostic laboratories. To date the Real-time Polymerase Chain reaction (RT-PCR) is the mainstay for diagnosis of MERS-CoV. RT-PCR has limitations, including a long turnaround time and lack of common measurements and correlations with Viral Load (VL). It is recommended to screen for MERS-CoV using RT-PCR of the upstream of envelope gene (upE) followed by confirmation of the presence of one of the following genes; open reading frame 1A, 1B genes or nucleocapsid (N) gene. Scientists are looking to implement viral sequencing on all negative samples by RT-PCR and they beleive that can be exposed to another level of testing using sequencing of the RNA-dependent RNA polymerase (RdRp) gene or N gene and in this case a positive result is diagnostic. It is also very important to maintain a contineous and random sequencing for MERS-Cov samples to be able to pick early viral mutations. Serological assays still not widely or routinely performed, and a lot of studies looking to implement such method in routine patient's testings.

DOI: 10.1016/j.jiph.2016.04.005
PubMed: 27106390

Links to Exploration step

pubmed:27106390

Le document en format XML

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<author><name sortKey="Al Johani, Sameera" sort="Al Johani, Sameera" uniqKey="Al Johani S" first="Sameera" last="Al Johani">Sameera Al Johani</name>
<affiliation><nlm:affiliation>Department of Pathology and Laboratory Medicine, King Saud University for Health Sciences, Riyadh, Saudi Arabia. Electronic address: johanis@ngha.med.sa.</nlm:affiliation>
</affiliation>
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<author><name sortKey="Hajeer, Ali H" sort="Hajeer, Ali H" uniqKey="Hajeer A" first="Ali H" last="Hajeer">Ali H. Hajeer</name>
<affiliation><nlm:affiliation>Department of Pathology and Laboratory Medicine, King Saud University for Health Sciences, Riyadh, Saudi Arabia.</nlm:affiliation>
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<front><div type="abstract" xml:lang="en">Diagnosis of MERS-Cov still a major concern in most of daignostic laboratories. To date the Real-time Polymerase Chain reaction (RT-PCR) is the mainstay for diagnosis of MERS-CoV. RT-PCR has limitations, including a long turnaround time and lack of common measurements and correlations with Viral Load (VL). It is recommended to screen for MERS-CoV using RT-PCR of the upstream of envelope gene (upE) followed by confirmation of the presence of one of the following genes; open reading frame 1A, 1B genes or nucleocapsid (N) gene. Scientists are looking to implement viral sequencing on all negative samples by RT-PCR and they beleive that can be exposed to another level of testing using sequencing of the RNA-dependent RNA polymerase (RdRp) gene or N gene and in this case a positive result is diagnostic. It is also very important to maintain a contineous and random sequencing for MERS-Cov samples to be able to pick early viral mutations. Serological assays still not widely or routinely performed, and a lot of studies looking to implement such method in routine patient's testings. </div>
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<Abstract><AbstractText>Diagnosis of MERS-Cov still a major concern in most of daignostic laboratories. To date the Real-time Polymerase Chain reaction (RT-PCR) is the mainstay for diagnosis of MERS-CoV. RT-PCR has limitations, including a long turnaround time and lack of common measurements and correlations with Viral Load (VL). It is recommended to screen for MERS-CoV using RT-PCR of the upstream of envelope gene (upE) followed by confirmation of the presence of one of the following genes; open reading frame 1A, 1B genes or nucleocapsid (N) gene. Scientists are looking to implement viral sequencing on all negative samples by RT-PCR and they beleive that can be exposed to another level of testing using sequencing of the RNA-dependent RNA polymerase (RdRp) gene or N gene and in this case a positive result is diagnostic. It is also very important to maintain a contineous and random sequencing for MERS-Cov samples to be able to pick early viral mutations. Serological assays still not widely or routinely performed, and a lot of studies looking to implement such method in routine patient's testings. </AbstractText>
<CopyrightInformation>Copyright © 2016 King Saud Bin Abdulaziz University for Health Sciences. Published by Elsevier Ltd. All rights reserved.</CopyrightInformation>
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MERS-CoV diagnosis: An update.

MERS-CoV diagnosis: An update.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

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