Spike gene deletion quasispecies in serum of patient with acute MERS-CoV infection.
Identifieur interne : 001016 ( PubMed/Corpus ); précédent : 001015; suivant : 001017Spike gene deletion quasispecies in serum of patient with acute MERS-CoV infection.
Auteurs : Xiaoyan Lu ; Lori A. Rowe ; Michael Frace ; James Stevens ; Glen R. Abedi ; Osman Elnile ; Taleb Banassir ; Malak Al-Masri ; John T. Watson ; Abdullah Assiri ; Dean D. ErdmanSource :
- Journal of medical virology [ 1096-9071 ] ; 2017.
English descriptors
- KwdEn :
- Coronavirus Infections (epidemiology), Coronavirus Infections (virology), Disease Outbreaks, Genetic Variation, Humans, Middle East Respiratory Syndrome Coronavirus (classification), Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Saudi Arabia (epidemiology), Sequence Deletion, Serum (virology), Spike Glycoprotein, Coronavirus (genetics).
- MESH :
- chemical , genetics : Spike Glycoprotein, Coronavirus.
- classification : Middle East Respiratory Syndrome Coronavirus.
- epidemiology : Coronavirus Infections, Saudi Arabia.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- virology : Coronavirus Infections, Serum.
- Disease Outbreaks, Genetic Variation, Humans, Sequence Deletion.
Abstract
The spike glycoprotein of the Middle East respiratory coronavirus (MERS-CoV) facilitates receptor binding and cell entry. During investigation of a multi-facility outbreak of MERS-CoV in Taif, Saudi Arabia, we identified a mixed population of wild-type and variant sequences with a large 530 nucleotide deletion in the spike gene from the serum of one patient. The out of frame deletion predicted loss of most of the S2 subunit of the spike protein leaving the S1 subunit with an intact receptor binding domain. This finding documents human infection with a novel genetic variant of MERS-CoV present as a quasispecies. J. Med. Virol. 89:542-545, 2017. © 2016 Wiley Periodicals, Inc.
DOI: 10.1002/jmv.24652
PubMed: 27486688
Links to Exploration step
pubmed:27486688Le document en format XML
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Rowe</name><affiliation><nlm:affiliation>Centers for Disease Control and Prevention, Atlanta, Georgia.</nlm:affiliation></affiliation></author><author><name sortKey="Frace, Michael" sort="Frace, Michael" uniqKey="Frace M" first="Michael" last="Frace">Michael Frace</name><affiliation><nlm:affiliation>Centers for Disease Control and Prevention, Atlanta, Georgia.</nlm:affiliation></affiliation></author><author><name sortKey="Stevens, James" sort="Stevens, James" uniqKey="Stevens J" first="James" last="Stevens">James Stevens</name><affiliation><nlm:affiliation>Centers for Disease Control and Prevention, Atlanta, Georgia.</nlm:affiliation></affiliation></author><author><name sortKey="Abedi, Glen R" sort="Abedi, Glen R" uniqKey="Abedi G" first="Glen R" last="Abedi">Glen R. Abedi</name><affiliation><nlm:affiliation>Centers for Disease Control and Prevention, Atlanta, Georgia.</nlm:affiliation></affiliation></author><author><name sortKey="Elnile, Osman" sort="Elnile, Osman" uniqKey="Elnile O" first="Osman" last="Elnile">Osman Elnile</name><affiliation><nlm:affiliation>Ministry of Health, Riyadh, Saudi Arabia.</nlm:affiliation></affiliation></author><author><name sortKey="Banassir, Taleb" sort="Banassir, Taleb" uniqKey="Banassir T" first="Taleb" last="Banassir">Taleb Banassir</name><affiliation><nlm:affiliation>Ministry of Health, Riyadh, Saudi Arabia.</nlm:affiliation></affiliation></author><author><name sortKey="Al Masri, Malak" sort="Al Masri, Malak" uniqKey="Al Masri M" first="Malak" last="Al-Masri">Malak Al-Masri</name><affiliation><nlm:affiliation>Ministry of Health, Riyadh, Saudi Arabia.</nlm:affiliation></affiliation></author><author><name sortKey="Watson, John T" sort="Watson, John T" uniqKey="Watson J" first="John T" last="Watson">John T. Watson</name><affiliation><nlm:affiliation>Centers for Disease Control and Prevention, Atlanta, Georgia.</nlm:affiliation></affiliation></author><author><name sortKey="Assiri, Abdullah" sort="Assiri, Abdullah" uniqKey="Assiri A" first="Abdullah" last="Assiri">Abdullah Assiri</name><affiliation><nlm:affiliation>Ministry of Health, Riyadh, Saudi Arabia.</nlm:affiliation></affiliation></author><author><name sortKey="Erdman, Dean D" sort="Erdman, Dean D" uniqKey="Erdman D" first="Dean D" last="Erdman">Dean D. Erdman</name><affiliation><nlm:affiliation>Centers for Disease Control and Prevention, Atlanta, Georgia.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:27486688</idno><idno type="pmid">27486688</idno><idno type="doi">10.1002/jmv.24652</idno><idno type="wicri:Area/PubMed/Corpus">001016</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001016</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Spike gene deletion quasispecies in serum of patient with acute MERS-CoV infection.</title><author><name sortKey="Lu, Xiaoyan" sort="Lu, Xiaoyan" uniqKey="Lu X" first="Xiaoyan" last="Lu">Xiaoyan Lu</name><affiliation><nlm:affiliation>Centers for Disease Control and Prevention, Atlanta, Georgia.</nlm:affiliation></affiliation></author><author><name sortKey="Rowe, Lori A" sort="Rowe, Lori A" uniqKey="Rowe L" first="Lori A" last="Rowe">Lori A. Rowe</name><affiliation><nlm:affiliation>Centers for Disease Control and Prevention, Atlanta, Georgia.</nlm:affiliation></affiliation></author><author><name sortKey="Frace, Michael" sort="Frace, Michael" uniqKey="Frace M" first="Michael" last="Frace">Michael Frace</name><affiliation><nlm:affiliation>Centers for Disease Control and Prevention, Atlanta, Georgia.</nlm:affiliation></affiliation></author><author><name sortKey="Stevens, James" sort="Stevens, James" uniqKey="Stevens J" first="James" last="Stevens">James Stevens</name><affiliation><nlm:affiliation>Centers for Disease Control and Prevention, Atlanta, Georgia.</nlm:affiliation></affiliation></author><author><name sortKey="Abedi, Glen R" sort="Abedi, Glen R" uniqKey="Abedi G" first="Glen R" last="Abedi">Glen R. Abedi</name><affiliation><nlm:affiliation>Centers for Disease Control and Prevention, Atlanta, Georgia.</nlm:affiliation></affiliation></author><author><name sortKey="Elnile, Osman" sort="Elnile, Osman" uniqKey="Elnile O" first="Osman" last="Elnile">Osman Elnile</name><affiliation><nlm:affiliation>Ministry of Health, Riyadh, Saudi Arabia.</nlm:affiliation></affiliation></author><author><name sortKey="Banassir, Taleb" sort="Banassir, Taleb" uniqKey="Banassir T" first="Taleb" last="Banassir">Taleb Banassir</name><affiliation><nlm:affiliation>Ministry of Health, Riyadh, Saudi Arabia.</nlm:affiliation></affiliation></author><author><name sortKey="Al Masri, Malak" sort="Al Masri, Malak" uniqKey="Al Masri M" first="Malak" last="Al-Masri">Malak Al-Masri</name><affiliation><nlm:affiliation>Ministry of Health, Riyadh, Saudi Arabia.</nlm:affiliation></affiliation></author><author><name sortKey="Watson, John T" sort="Watson, John T" uniqKey="Watson J" first="John T" last="Watson">John T. Watson</name><affiliation><nlm:affiliation>Centers for Disease Control and Prevention, Atlanta, Georgia.</nlm:affiliation></affiliation></author><author><name sortKey="Assiri, Abdullah" sort="Assiri, Abdullah" uniqKey="Assiri A" first="Abdullah" last="Assiri">Abdullah Assiri</name><affiliation><nlm:affiliation>Ministry of Health, Riyadh, Saudi Arabia.</nlm:affiliation></affiliation></author><author><name sortKey="Erdman, Dean D" sort="Erdman, Dean D" uniqKey="Erdman D" first="Dean D" last="Erdman">Dean D. Erdman</name><affiliation><nlm:affiliation>Centers for Disease Control and Prevention, Atlanta, Georgia.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Journal of medical virology</title><idno type="eISSN">1096-9071</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus Infections (epidemiology)</term><term>Coronavirus Infections (virology)</term><term>Disease Outbreaks</term><term>Genetic Variation</term><term>Humans</term><term>Middle East Respiratory Syndrome Coronavirus (classification)</term><term>Middle East Respiratory Syndrome Coronavirus (genetics)</term><term>Middle East Respiratory Syndrome Coronavirus (isolation & purification)</term><term>Saudi Arabia (epidemiology)</term><term>Sequence Deletion</term><term>Serum (virology)</term><term>Spike Glycoprotein, Coronavirus (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Coronavirus Infections</term><term>Saudi Arabia</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term><term>Serum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Disease Outbreaks</term><term>Genetic Variation</term><term>Humans</term><term>Sequence Deletion</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The spike glycoprotein of the Middle East respiratory coronavirus (MERS-CoV) facilitates receptor binding and cell entry. During investigation of a multi-facility outbreak of MERS-CoV in Taif, Saudi Arabia, we identified a mixed population of wild-type and variant sequences with a large 530 nucleotide deletion in the spike gene from the serum of one patient. The out of frame deletion predicted loss of most of the S2 subunit of the spike protein leaving the S1 subunit with an intact receptor binding domain. This finding documents human infection with a novel genetic variant of MERS-CoV present as a quasispecies. J. Med. Virol. 89:542-545, 2017. © 2016 Wiley Periodicals, Inc.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27486688</PMID><DateCompleted><Year>2017</Year><Month>09</Month><Day>07</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>03</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-9071</ISSN><JournalIssue CitedMedium="Internet"><Volume>89</Volume><Issue>3</Issue><PubDate><Year>2017</Year><Month>03</Month></PubDate></JournalIssue><Title>Journal of medical virology</Title><ISOAbbreviation>J. Med. Virol.</ISOAbbreviation></Journal><ArticleTitle>Spike gene deletion quasispecies in serum of patient with acute MERS-CoV infection.</ArticleTitle><Pagination><MedlinePgn>542-545</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/jmv.24652</ELocationID><Abstract><AbstractText>The spike glycoprotein of the Middle East respiratory coronavirus (MERS-CoV) facilitates receptor binding and cell entry. During investigation of a multi-facility outbreak of MERS-CoV in Taif, Saudi Arabia, we identified a mixed population of wild-type and variant sequences with a large 530 nucleotide deletion in the spike gene from the serum of one patient. The out of frame deletion predicted loss of most of the S2 subunit of the spike protein leaving the S1 subunit with an intact receptor binding domain. This finding documents human infection with a novel genetic variant of MERS-CoV present as a quasispecies. J. Med. Virol. 89:542-545, 2017. © 2016 Wiley Periodicals, Inc.</AbstractText><CopyrightInformation>© 2016 Wiley Periodicals, Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Xiaoyan</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Centers for Disease Control and Prevention, Atlanta, Georgia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rowe</LastName><ForeName>Lori A</ForeName><Initials>LA</Initials><AffiliationInfo><Affiliation>Centers for Disease Control and Prevention, Atlanta, Georgia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Frace</LastName><ForeName>Michael</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Centers for Disease Control and Prevention, Atlanta, Georgia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Stevens</LastName><ForeName>James</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Centers for Disease Control and Prevention, Atlanta, Georgia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Abedi</LastName><ForeName>Glen R</ForeName><Initials>GR</Initials><AffiliationInfo><Affiliation>Centers for Disease Control and Prevention, Atlanta, Georgia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Elnile</LastName><ForeName>Osman</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Ministry of Health, Riyadh, Saudi Arabia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Banassir</LastName><ForeName>Taleb</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Ministry of Health, Riyadh, Saudi Arabia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Al-Masri</LastName><ForeName>Malak</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Ministry of Health, Riyadh, Saudi Arabia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Watson</LastName><ForeName>John T</ForeName><Initials>JT</Initials><AffiliationInfo><Affiliation>Centers for Disease Control and Prevention, Atlanta, Georgia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Assiri</LastName><ForeName>Abdullah</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Ministry of Health, Riyadh, Saudi Arabia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Erdman</LastName><ForeName>Dean D</ForeName><Initials>DD</Initials><AffiliationInfo><Affiliation>Centers for Disease Control and Prevention, Atlanta, Georgia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>08</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Med Virol</MedlineTA><NlmUniqueID>7705876</NlmUniqueID><ISSNLinking>0146-6615</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004196" MajorTopicYN="N">Disease Outbreaks</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014644" MajorTopicYN="Y">Genetic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D065207" MajorTopicYN="N">Middle East Respiratory Syndrome Coronavirus</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012529" MajorTopicYN="N">Saudi Arabia</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017384" MajorTopicYN="Y">Sequence Deletion</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D044967" MajorTopicYN="N">Serum</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D064370" MajorTopicYN="N">Spike Glycoprotein, Coronavirus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">MERS-CoV</Keyword><Keyword MajorTopicYN="Y">coronavirus</Keyword><Keyword MajorTopicYN="Y">genome</Keyword><Keyword MajorTopicYN="Y">spike gene</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>07</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>8</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>9</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>8</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27486688</ArticleId><ArticleId IdType="doi">10.1002/jmv.24652</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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