Novel chimeric virus-like particles vaccine displaying MERS-CoV receptor-binding domain induce specific humoral and cellular immune response in mice.
Identifieur interne : 000E28 ( PubMed/Corpus ); précédent : 000E27; suivant : 000E29Novel chimeric virus-like particles vaccine displaying MERS-CoV receptor-binding domain induce specific humoral and cellular immune response in mice.
Auteurs : Chong Wang ; Xuexing Zheng ; Weiwei Gai ; Gary Wong ; Hualei Wang ; Hongli Jin ; Na Feng ; Yongkun Zhao ; Weijiao Zhang ; Nan Li ; Guoxing Zhao ; Junfu Li ; Jinghua Yan ; Yuwei Gao ; Guixue Hu ; Songtao Yang ; Xianzhu XiaSource :
- Antiviral research [ 1872-9096 ] ; 2017.
English descriptors
- KwdEn :
- Adjuvants, Immunologic, Animals, Antibodies, Neutralizing (blood), Antibodies, Viral (blood), Capsid Proteins (genetics), Capsid Proteins (immunology), Chimera, Coronavirus Infections (prevention & control), Cytokines (immunology), Cytokines (metabolism), Erythrocytes (virology), Immunity, Cellular, Immunity, Humoral, Mice, Middle East Respiratory Syndrome Coronavirus (genetics), Parvovirus, Canine (genetics), Protein Binding, Swine, Th1 Cells (immunology), Th2 Cells (immunology), Vaccines, Virus-Like Particle (genetics), Vaccines, Virus-Like Particle (immunology), Viral Vaccines (administration & dosage), Viral Vaccines (immunology).
- MESH :
- chemical , administration & dosage : Viral Vaccines.
- chemical , blood : Antibodies, Neutralizing, Antibodies, Viral.
- chemical , genetics : Capsid Proteins, Vaccines, Virus-Like Particle.
- chemical , immunology : Capsid Proteins, Cytokines, Vaccines, Virus-Like Particle, Viral Vaccines.
- chemical , metabolism : Cytokines.
- chemical : Adjuvants, Immunologic.
- genetics : Middle East Respiratory Syndrome Coronavirus, Parvovirus, Canine.
- immunology : Th1 Cells, Th2 Cells.
- prevention & control : Coronavirus Infections.
- virology : Erythrocytes.
- Animals, Chimera, Immunity, Cellular, Immunity, Humoral, Mice, Protein Binding, Swine.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) has continued spreading since its emergence in 2012 with a mortality rate of 35.6%, and is a potential pandemic threat. Prophylactics and therapies are urgently needed to address this public health problem. We report here the efficacy of a vaccine consisting of chimeric virus-like particles (VLP) expressing the receptor binding domain (RBD) of MERS-CoV. In this study, a fusion of the canine parvovirus (CPV) VP2 structural protein gene with the RBD of MERS-CoV can self-assemble into chimeric, spherical VLP (sVLP). sVLP retained certain parvovirus characteristics, such as the ability to agglutinate pig erythrocytes, and structural morphology similar to CPV virions. Immunization with sVLP induced RBD-specific humoral and cellular immune responses in mice. sVLP-specific antisera from these animals were able to prevent pseudotyped MERS-CoV entry into susceptible cells, with neutralizing antibody titers reaching 1: 320. IFN-γ, IL-4 and IL-2 secreting cells induced by the RBD were detected in the splenocytes of vaccinated mice by ELISpot. Furthermore, mice inoculated with sVLP or an adjuvanted sVLP vaccine elicited T-helper 1 (Th1) and T-helper 2 (Th2) cell-mediated immunity. Our study demonstrates that sVLP displaying the RBD of MERS-CoV are promising prophylactic candidates against MERS-CoV in a potential outbreak situation.
DOI: 10.1016/j.antiviral.2016.12.019
PubMed: 28040513
Links to Exploration step
pubmed:28040513Le document en format XML
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<author><name sortKey="Gai, Weiwei" sort="Gai, Weiwei" uniqKey="Gai W" first="Weiwei" last="Gai">Weiwei Gai</name>
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<author><name sortKey="Zhao, Guoxing" sort="Zhao, Guoxing" uniqKey="Zhao G" first="Guoxing" last="Zhao">Guoxing Zhao</name>
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<author><name sortKey="Li, Junfu" sort="Li, Junfu" uniqKey="Li J" first="Junfu" last="Li">Junfu Li</name>
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<author><name sortKey="Yan, Jinghua" sort="Yan, Jinghua" uniqKey="Yan J" first="Jinghua" last="Yan">Jinghua Yan</name>
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<author><name sortKey="Gao, Yuwei" sort="Gao, Yuwei" uniqKey="Gao Y" first="Yuwei" last="Gao">Yuwei Gao</name>
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<author><name sortKey="Hu, Guixue" sort="Hu, Guixue" uniqKey="Hu G" first="Guixue" last="Hu">Guixue Hu</name>
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<author><name sortKey="Xia, Xianzhu" sort="Xia, Xianzhu" uniqKey="Xia X" first="Xianzhu" last="Xia">Xianzhu Xia</name>
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<front><div type="abstract" xml:lang="en">Middle East respiratory syndrome coronavirus (MERS-CoV) has continued spreading since its emergence in 2012 with a mortality rate of 35.6%, and is a potential pandemic threat. Prophylactics and therapies are urgently needed to address this public health problem. We report here the efficacy of a vaccine consisting of chimeric virus-like particles (VLP) expressing the receptor binding domain (RBD) of MERS-CoV. In this study, a fusion of the canine parvovirus (CPV) VP2 structural protein gene with the RBD of MERS-CoV can self-assemble into chimeric, spherical VLP (sVLP). sVLP retained certain parvovirus characteristics, such as the ability to agglutinate pig erythrocytes, and structural morphology similar to CPV virions. Immunization with sVLP induced RBD-specific humoral and cellular immune responses in mice. sVLP-specific antisera from these animals were able to prevent pseudotyped MERS-CoV entry into susceptible cells, with neutralizing antibody titers reaching 1: 320. IFN-γ, IL-4 and IL-2 secreting cells induced by the RBD were detected in the splenocytes of vaccinated mice by ELISpot. Furthermore, mice inoculated with sVLP or an adjuvanted sVLP vaccine elicited T-helper 1 (Th1) and T-helper 2 (Th2) cell-mediated immunity. Our study demonstrates that sVLP displaying the RBD of MERS-CoV are promising prophylactic candidates against MERS-CoV in a potential outbreak situation.</div>
</front>
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<DateCompleted><Year>2017</Year>
<Month>12</Month>
<Day>19</Day>
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<DateRevised><Year>2020</Year>
<Month>04</Month>
<Day>07</Day>
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<JournalIssue CitedMedium="Internet"><Volume>140</Volume>
<PubDate><Year>2017</Year>
<Month>04</Month>
</PubDate>
</JournalIssue>
<Title>Antiviral research</Title>
<ISOAbbreviation>Antiviral Res.</ISOAbbreviation>
</Journal>
<ArticleTitle>Novel chimeric virus-like particles vaccine displaying MERS-CoV receptor-binding domain induce specific humoral and cellular immune response in mice.</ArticleTitle>
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<Abstract><AbstractText>Middle East respiratory syndrome coronavirus (MERS-CoV) has continued spreading since its emergence in 2012 with a mortality rate of 35.6%, and is a potential pandemic threat. Prophylactics and therapies are urgently needed to address this public health problem. We report here the efficacy of a vaccine consisting of chimeric virus-like particles (VLP) expressing the receptor binding domain (RBD) of MERS-CoV. In this study, a fusion of the canine parvovirus (CPV) VP2 structural protein gene with the RBD of MERS-CoV can self-assemble into chimeric, spherical VLP (sVLP). sVLP retained certain parvovirus characteristics, such as the ability to agglutinate pig erythrocytes, and structural morphology similar to CPV virions. Immunization with sVLP induced RBD-specific humoral and cellular immune responses in mice. sVLP-specific antisera from these animals were able to prevent pseudotyped MERS-CoV entry into susceptible cells, with neutralizing antibody titers reaching 1: 320. IFN-γ, IL-4 and IL-2 secreting cells induced by the RBD were detected in the splenocytes of vaccinated mice by ELISpot. Furthermore, mice inoculated with sVLP or an adjuvanted sVLP vaccine elicited T-helper 1 (Th1) and T-helper 2 (Th2) cell-mediated immunity. Our study demonstrates that sVLP displaying the RBD of MERS-CoV are promising prophylactic candidates against MERS-CoV in a potential outbreak situation.</AbstractText>
<CopyrightInformation>Copyright © 2017 Elsevier B.V. All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Chong</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Zheng</LastName>
<ForeName>Xuexing</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>School of Public Health, Shandong University, Jinan, China.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Gai</LastName>
<ForeName>Weiwei</ForeName>
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</AffiliationInfo>
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</AffiliationInfo>
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<Initials>H</Initials>
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<Author ValidYN="Y"><LastName>Feng</LastName>
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<Initials>N</Initials>
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