Multiplex Paper-Based Colorimetric DNA Sensor Using Pyrrolidinyl Peptide Nucleic Acid-Induced AgNPs Aggregation for Detecting MERS-CoV, MTB, and HPV Oligonucleotides.
Identifieur interne : 000D24 ( PubMed/Corpus ); précédent : 000D23; suivant : 000D25Multiplex Paper-Based Colorimetric DNA Sensor Using Pyrrolidinyl Peptide Nucleic Acid-Induced AgNPs Aggregation for Detecting MERS-CoV, MTB, and HPV Oligonucleotides.
Auteurs : Prinjaporn Teengam ; Weena Siangproh ; Adisorn Tuantranont ; Tirayut Vilaivan ; Orawon Chailapakul ; Charles S. HenrySource :
- Analytical chemistry [ 1520-6882 ] ; 2017.
English descriptors
- KwdEn :
- Base Sequence, Colorimetry (methods), DNA (analysis), DNA, Bacterial (analysis), DNA, Viral (analysis), Humans, Image Processing, Computer-Assisted, Limit of Detection, Metal Nanoparticles (chemistry), Middle East Respiratory Syndrome Coronavirus (genetics), Mycobacterium tuberculosis (genetics), Nucleic Acid Hybridization, Oligonucleotides (chemistry), Oligonucleotides (metabolism), Paper, Papillomaviridae (genetics), Peptide Nucleic Acids (chemistry), Silver (chemistry).
- MESH :
- chemical , analysis : DNA, DNA, Bacterial, DNA, Viral.
- chemistry : Metal Nanoparticles, Oligonucleotides, Peptide Nucleic Acids, Silver.
- genetics : Middle East Respiratory Syndrome Coronavirus, Mycobacterium tuberculosis, Papillomaviridae.
- chemical , metabolism : Oligonucleotides.
- methods : Colorimetry.
- Base Sequence, Humans, Image Processing, Computer-Assisted, Limit of Detection, Nucleic Acid Hybridization, Paper.
Abstract
The development of simple fluorescent and colorimetric assays that enable point-of-care DNA and RNA detection has been a topic of significant research because of the utility of such assays in resource limited settings. The most common motifs utilize hybridization to a complementary detection strand coupled with a sensitive reporter molecule. Here, a paper-based colorimetric assay for DNA detection based on pyrrolidinyl peptide nucleic acid (acpcPNA)-induced nanoparticle aggregation is reported as an alternative to traditional colorimetric approaches. PNA probes are an attractive alternative to DNA and RNA probes because they are chemically and biologically stable, easily synthesized, and hybridize efficiently with the complementary DNA strands. The acpcPNA probe contains a single positive charge from the lysine at C-terminus and causes aggregation of citrate anion-stabilized silver nanoparticles (AgNPs) in the absence of complementary DNA. In the presence of target DNA, formation of the anionic DNA-acpcPNA duplex results in dispersion of the AgNPs as a result of electrostatic repulsion, giving rise to a detectable color change. Factors affecting the sensitivity and selectivity of this assay were investigated, including ionic strength, AgNP concentration, PNA concentration, and DNA strand mismatches. The method was used for screening of synthetic Middle East respiratory syndrome coronavirus (MERS-CoV), Mycobacterium tuberculosis (MTB), and human papillomavirus (HPV) DNA based on a colorimetric paper-based analytical device developed using the aforementioned principle. The oligonucleotide targets were detected by measuring the color change of AgNPs, giving detection limits of 1.53 (MERS-CoV), 1.27 (MTB), and 1.03 nM (HPV). The acpcPNA probe exhibited high selectivity for the complementary oligonucleotides over single-base-mismatch, two-base-mismatch, and noncomplementary DNA targets. The proposed paper-based colorimetric DNA sensor has potential to be an alternative approach for simple, rapid, sensitive, and selective DNA detection.
DOI: 10.1021/acs.analchem.7b00255
PubMed: 28394582
Links to Exploration step
pubmed:28394582Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Multiplex Paper-Based Colorimetric DNA Sensor Using Pyrrolidinyl Peptide Nucleic Acid-Induced AgNPs Aggregation for Detecting MERS-CoV, MTB, and HPV Oligonucleotides.</title><author><name sortKey="Teengam, Prinjaporn" sort="Teengam, Prinjaporn" uniqKey="Teengam P" first="Prinjaporn" last="Teengam">Prinjaporn Teengam</name></author><author><name sortKey="Siangproh, Weena" sort="Siangproh, Weena" uniqKey="Siangproh W" first="Weena" last="Siangproh">Weena Siangproh</name><affiliation><nlm:affiliation>Department of Chemistry, Faculty of Science, Srinakharinwirot University , Bangkok, 10110, Thailand.</nlm:affiliation></affiliation></author><author><name sortKey="Tuantranont, Adisorn" sort="Tuantranont, Adisorn" uniqKey="Tuantranont A" first="Adisorn" last="Tuantranont">Adisorn Tuantranont</name><affiliation><nlm:affiliation>Nanoelectronics and MEMS Laboratory, National Electronics and Computer Technology Center , Pathumthani 12120, Thailand.</nlm:affiliation></affiliation></author><author><name sortKey="Vilaivan, Tirayut" sort="Vilaivan, Tirayut" uniqKey="Vilaivan T" first="Tirayut" last="Vilaivan">Tirayut Vilaivan</name></author><author><name sortKey="Chailapakul, Orawon" sort="Chailapakul, Orawon" uniqKey="Chailapakul O" first="Orawon" last="Chailapakul">Orawon Chailapakul</name></author><author><name sortKey="Henry, Charles S" sort="Henry, Charles S" uniqKey="Henry C" first="Charles S" last="Henry">Charles S. Henry</name><affiliation><nlm:affiliation>Departments of Chemistry and Chemical and Biological Engineering, Colorado State University , Fort Collins, Colorado 80523, United States.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28394582</idno><idno type="pmid">28394582</idno><idno type="doi">10.1021/acs.analchem.7b00255</idno><idno type="wicri:Area/PubMed/Corpus">000D24</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000D24</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Multiplex Paper-Based Colorimetric DNA Sensor Using Pyrrolidinyl Peptide Nucleic Acid-Induced AgNPs Aggregation for Detecting MERS-CoV, MTB, and HPV Oligonucleotides.</title><author><name sortKey="Teengam, Prinjaporn" sort="Teengam, Prinjaporn" uniqKey="Teengam P" first="Prinjaporn" last="Teengam">Prinjaporn Teengam</name></author><author><name sortKey="Siangproh, Weena" sort="Siangproh, Weena" uniqKey="Siangproh W" first="Weena" last="Siangproh">Weena Siangproh</name><affiliation><nlm:affiliation>Department of Chemistry, Faculty of Science, Srinakharinwirot University , Bangkok, 10110, Thailand.</nlm:affiliation></affiliation></author><author><name sortKey="Tuantranont, Adisorn" sort="Tuantranont, Adisorn" uniqKey="Tuantranont A" first="Adisorn" last="Tuantranont">Adisorn Tuantranont</name><affiliation><nlm:affiliation>Nanoelectronics and MEMS Laboratory, National Electronics and Computer Technology Center , Pathumthani 12120, Thailand.</nlm:affiliation></affiliation></author><author><name sortKey="Vilaivan, Tirayut" sort="Vilaivan, Tirayut" uniqKey="Vilaivan T" first="Tirayut" last="Vilaivan">Tirayut Vilaivan</name></author><author><name sortKey="Chailapakul, Orawon" sort="Chailapakul, Orawon" uniqKey="Chailapakul O" first="Orawon" last="Chailapakul">Orawon Chailapakul</name></author><author><name sortKey="Henry, Charles S" sort="Henry, Charles S" uniqKey="Henry C" first="Charles S" last="Henry">Charles S. Henry</name><affiliation><nlm:affiliation>Departments of Chemistry and Chemical and Biological Engineering, Colorado State University , Fort Collins, Colorado 80523, United States.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Analytical chemistry</title><idno type="eISSN">1520-6882</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>Colorimetry (methods)</term><term>DNA (analysis)</term><term>DNA, Bacterial (analysis)</term><term>DNA, Viral (analysis)</term><term>Humans</term><term>Image Processing, Computer-Assisted</term><term>Limit of Detection</term><term>Metal Nanoparticles (chemistry)</term><term>Middle East Respiratory Syndrome Coronavirus (genetics)</term><term>Mycobacterium tuberculosis (genetics)</term><term>Nucleic Acid Hybridization</term><term>Oligonucleotides (chemistry)</term><term>Oligonucleotides (metabolism)</term><term>Paper</term><term>Papillomaviridae (genetics)</term><term>Peptide Nucleic Acids (chemistry)</term><term>Silver (chemistry)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA</term><term>DNA, Bacterial</term><term>DNA, Viral</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Metal Nanoparticles</term><term>Oligonucleotides</term><term>Peptide Nucleic Acids</term><term>Silver</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term><term>Mycobacterium tuberculosis</term><term>Papillomaviridae</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Oligonucleotides</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Colorimetry</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Humans</term><term>Image Processing, Computer-Assisted</term><term>Limit of Detection</term><term>Nucleic Acid Hybridization</term><term>Paper</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The development of simple fluorescent and colorimetric assays that enable point-of-care DNA and RNA detection has been a topic of significant research because of the utility of such assays in resource limited settings. The most common motifs utilize hybridization to a complementary detection strand coupled with a sensitive reporter molecule. Here, a paper-based colorimetric assay for DNA detection based on pyrrolidinyl peptide nucleic acid (acpcPNA)-induced nanoparticle aggregation is reported as an alternative to traditional colorimetric approaches. PNA probes are an attractive alternative to DNA and RNA probes because they are chemically and biologically stable, easily synthesized, and hybridize efficiently with the complementary DNA strands. The acpcPNA probe contains a single positive charge from the lysine at C-terminus and causes aggregation of citrate anion-stabilized silver nanoparticles (AgNPs) in the absence of complementary DNA. In the presence of target DNA, formation of the anionic DNA-acpcPNA duplex results in dispersion of the AgNPs as a result of electrostatic repulsion, giving rise to a detectable color change. Factors affecting the sensitivity and selectivity of this assay were investigated, including ionic strength, AgNP concentration, PNA concentration, and DNA strand mismatches. The method was used for screening of synthetic Middle East respiratory syndrome coronavirus (MERS-CoV), Mycobacterium tuberculosis (MTB), and human papillomavirus (HPV) DNA based on a colorimetric paper-based analytical device developed using the aforementioned principle. The oligonucleotide targets were detected by measuring the color change of AgNPs, giving detection limits of 1.53 (MERS-CoV), 1.27 (MTB), and 1.03 nM (HPV). The acpcPNA probe exhibited high selectivity for the complementary oligonucleotides over single-base-mismatch, two-base-mismatch, and noncomplementary DNA targets. The proposed paper-based colorimetric DNA sensor has potential to be an alternative approach for simple, rapid, sensitive, and selective DNA detection.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28394582</PMID><DateCompleted><Year>2019</Year><Month>02</Month><Day>15</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-6882</ISSN><JournalIssue CitedMedium="Internet"><Volume>89</Volume><Issue>10</Issue><PubDate><Year>2017</Year><Month>05</Month><Day>16</Day></PubDate></JournalIssue><Title>Analytical chemistry</Title><ISOAbbreviation>Anal. Chem.</ISOAbbreviation></Journal><ArticleTitle>Multiplex Paper-Based Colorimetric DNA Sensor Using Pyrrolidinyl Peptide Nucleic Acid-Induced AgNPs Aggregation for Detecting MERS-CoV, MTB, and HPV Oligonucleotides.</ArticleTitle><Pagination><MedlinePgn>5428-5435</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/acs.analchem.7b00255</ELocationID><Abstract><AbstractText>The development of simple fluorescent and colorimetric assays that enable point-of-care DNA and RNA detection has been a topic of significant research because of the utility of such assays in resource limited settings. The most common motifs utilize hybridization to a complementary detection strand coupled with a sensitive reporter molecule. Here, a paper-based colorimetric assay for DNA detection based on pyrrolidinyl peptide nucleic acid (acpcPNA)-induced nanoparticle aggregation is reported as an alternative to traditional colorimetric approaches. PNA probes are an attractive alternative to DNA and RNA probes because they are chemically and biologically stable, easily synthesized, and hybridize efficiently with the complementary DNA strands. The acpcPNA probe contains a single positive charge from the lysine at C-terminus and causes aggregation of citrate anion-stabilized silver nanoparticles (AgNPs) in the absence of complementary DNA. In the presence of target DNA, formation of the anionic DNA-acpcPNA duplex results in dispersion of the AgNPs as a result of electrostatic repulsion, giving rise to a detectable color change. Factors affecting the sensitivity and selectivity of this assay were investigated, including ionic strength, AgNP concentration, PNA concentration, and DNA strand mismatches. The method was used for screening of synthetic Middle East respiratory syndrome coronavirus (MERS-CoV), Mycobacterium tuberculosis (MTB), and human papillomavirus (HPV) DNA based on a colorimetric paper-based analytical device developed using the aforementioned principle. The oligonucleotide targets were detected by measuring the color change of AgNPs, giving detection limits of 1.53 (MERS-CoV), 1.27 (MTB), and 1.03 nM (HPV). The acpcPNA probe exhibited high selectivity for the complementary oligonucleotides over single-base-mismatch, two-base-mismatch, and noncomplementary DNA targets. The proposed paper-based colorimetric DNA sensor has potential to be an alternative approach for simple, rapid, sensitive, and selective DNA detection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Teengam</LastName><ForeName>Prinjaporn</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Siangproh</LastName><ForeName>Weena</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Chemistry, Faculty of Science, Srinakharinwirot University , Bangkok, 10110, Thailand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tuantranont</LastName><ForeName>Adisorn</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Nanoelectronics and MEMS Laboratory, National Electronics and Computer Technology Center , Pathumthani 12120, Thailand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vilaivan</LastName><ForeName>Tirayut</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Chailapakul</LastName><ForeName>Orawon</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Henry</LastName><ForeName>Charles S</ForeName><Initials>CS</Initials><Identifier Source="ORCID">0000-0002-8671-7728</Identifier><AffiliationInfo><Affiliation>Departments of Chemistry and Chemical and Biological Engineering, Colorado State University , Fort Collins, Colorado 80523, United States.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>04</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Anal 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Detection</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053768" MajorTopicYN="N">Metal Nanoparticles</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D065207" MajorTopicYN="N">Middle East Respiratory Syndrome Coronavirus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009169" MajorTopicYN="N">Mycobacterium tuberculosis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009693" MajorTopicYN="N">Nucleic Acid Hybridization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009841" MajorTopicYN="N">Oligonucleotides</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName 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