A novel neutralizing monoclonal antibody targeting the N-terminal domain of the MERS-CoV spike protein.
Identifieur interne : 000C85 ( PubMed/Corpus ); précédent : 000C84; suivant : 000C86A novel neutralizing monoclonal antibody targeting the N-terminal domain of the MERS-CoV spike protein.
Auteurs : Yingzhu Chen ; Shuai Lu ; Hao Jia ; Yao Deng ; Jianfang Zhou ; Baoying Huang ; Yueyang Yu ; Jiaming Lan ; Wenling Wang ; Yongliang Lou ; Kun Qin ; Wenjie TanSource :
- Emerging microbes & infections [ 2222-1751 ] ; 2017.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (immunology), Antibodies, Neutralizing (immunology), Antibodies, Viral (immunology), Chlorocebus aethiops, Coronavirus Infections (therapy), Humans, Immunization, Mice, Middle East Respiratory Syndrome Coronavirus (chemistry), Middle East Respiratory Syndrome Coronavirus (immunology), Models, Molecular, Protein Binding, Receptors, Virus, Spike Glycoprotein, Coronavirus (administration & dosage), Spike Glycoprotein, Coronavirus (chemistry), Spike Glycoprotein, Coronavirus (genetics), Spike Glycoprotein, Coronavirus (immunology), Vero Cells, Viral Vaccines.
- MESH :
- chemical , administration & dosage : Spike Glycoprotein, Coronavirus.
- chemical , chemistry : Spike Glycoprotein, Coronavirus.
- chemical , genetics : Spike Glycoprotein, Coronavirus.
- chemical , immunology : Antibodies, Monoclonal, Antibodies, Neutralizing, Antibodies, Viral, Spike Glycoprotein, Coronavirus.
- chemistry : Middle East Respiratory Syndrome Coronavirus.
- immunology : Middle East Respiratory Syndrome Coronavirus.
- therapy : Coronavirus Infections.
- Animals, Chlorocebus aethiops, Humans, Immunization, Mice, Models, Molecular, Protein Binding, Receptors, Virus, Vero Cells, Viral Vaccines.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) has caused fatal infections, some through hospital-acquired transmission, in affected regions since its emergence in 2012. Although the virus is not pandemic among humans, it poses a great threat to public health due to its zoonotic origin. Thus, both preventative and therapeutic countermeasures are urgently needed. In this study, we discovered a panel of neutralizing monoclonal antibodies (mAbs) against MERS-CoV, which mapped to a wide range of regions on the spike (S) protein of the virus. In addition to mAbs with neutralizing epitopes located on the receptor-binding domain, one mAb, 5F9, which binds to the N-terminal domain (NTD) of the MERS-CoV S1 subunit, showed efficient neutralizing activity against the wild-type MERS-CoV strain EMC/2012, with a half maximal inhibitory concentration of 0.2 μg/mL. We concluded that a novel neutralizing epitope for MERS-CoV also resides on the NTD of the S protein, indicating that the NTD might be important during the viral infection process. Our findings have significant implications for further vaccine design and for the development of prophylactic and therapeutic monoclonal immunotherapies against MERS-CoV infection.
DOI: 10.1038/emi.2017.18
PubMed: 28536429
Links to Exploration step
pubmed:28536429Le document en format XML
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Life Science and Key Laboratory of Medical Virology, Wenzhou 325035, China.</nlm:affiliation></affiliation></author><author><name sortKey="Deng, Yao" sort="Deng, Yao" uniqKey="Deng Y" first="Yao" last="Deng">Yao Deng</name><affiliation><nlm:affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhou, Jianfang" sort="Zhou, Jianfang" uniqKey="Zhou J" first="Jianfang" last="Zhou">Jianfang Zhou</name><affiliation><nlm:affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</nlm:affiliation></affiliation></author><author><name sortKey="Huang, Baoying" sort="Huang, Baoying" uniqKey="Huang B" first="Baoying" last="Huang">Baoying 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China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Wenling" sort="Wang, Wenling" uniqKey="Wang W" first="Wenling" last="Wang">Wenling Wang</name><affiliation><nlm:affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</nlm:affiliation></affiliation></author><author><name sortKey="Lou, Yongliang" sort="Lou, Yongliang" uniqKey="Lou Y" first="Yongliang" last="Lou">Yongliang Lou</name><affiliation><nlm:affiliation>School of Laboratory Medicine and Life Science and Key Laboratory of Medical Virology, Wenzhou 325035, China.</nlm:affiliation></affiliation></author><author><name sortKey="Qin, Kun" sort="Qin, Kun" uniqKey="Qin K" first="Kun" last="Qin">Kun Qin</name><affiliation><nlm:affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</nlm:affiliation></affiliation></author><author><name sortKey="Tan, Wenjie" sort="Tan, Wenjie" uniqKey="Tan W" first="Wenjie" last="Tan">Wenjie Tan</name><affiliation><nlm:affiliation>School of Laboratory Medicine and Life Science and Key Laboratory of Medical Virology, Wenzhou 325035, China.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28536429</idno><idno type="pmid">28536429</idno><idno type="doi">10.1038/emi.2017.18</idno><idno type="wicri:Area/PubMed/Corpus">000C85</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000C85</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A novel neutralizing monoclonal antibody targeting the N-terminal domain of the MERS-CoV spike protein.</title><author><name sortKey="Chen, Yingzhu" sort="Chen, Yingzhu" uniqKey="Chen Y" first="Yingzhu" last="Chen">Yingzhu Chen</name><affiliation><nlm:affiliation>School of Laboratory Medicine and Life Science and Key Laboratory of Medical Virology, Wenzhou 325035, China.</nlm:affiliation></affiliation></author><author><name sortKey="Lu, Shuai" sort="Lu, Shuai" uniqKey="Lu S" first="Shuai" last="Lu">Shuai Lu</name><affiliation><nlm:affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</nlm:affiliation></affiliation></author><author><name sortKey="Jia, Hao" sort="Jia, Hao" uniqKey="Jia H" first="Hao" last="Jia">Hao Jia</name><affiliation><nlm:affiliation>School of Laboratory Medicine and Life Science and Key Laboratory of Medical Virology, Wenzhou 325035, China.</nlm:affiliation></affiliation></author><author><name sortKey="Deng, Yao" sort="Deng, Yao" uniqKey="Deng Y" first="Yao" last="Deng">Yao Deng</name><affiliation><nlm:affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhou, Jianfang" sort="Zhou, Jianfang" uniqKey="Zhou J" first="Jianfang" last="Zhou">Jianfang Zhou</name><affiliation><nlm:affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</nlm:affiliation></affiliation></author><author><name sortKey="Huang, Baoying" sort="Huang, Baoying" uniqKey="Huang B" first="Baoying" last="Huang">Baoying Huang</name><affiliation><nlm:affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yu, Yueyang" sort="Yu, Yueyang" uniqKey="Yu Y" first="Yueyang" last="Yu">Yueyang Yu</name><affiliation><nlm:affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</nlm:affiliation></affiliation></author><author><name sortKey="Lan, Jiaming" sort="Lan, Jiaming" uniqKey="Lan J" first="Jiaming" last="Lan">Jiaming Lan</name><affiliation><nlm:affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Wenling" sort="Wang, Wenling" uniqKey="Wang W" first="Wenling" last="Wang">Wenling Wang</name><affiliation><nlm:affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</nlm:affiliation></affiliation></author><author><name sortKey="Lou, Yongliang" sort="Lou, Yongliang" uniqKey="Lou Y" first="Yongliang" last="Lou">Yongliang Lou</name><affiliation><nlm:affiliation>School of Laboratory Medicine and Life Science and Key Laboratory of Medical Virology, Wenzhou 325035, China.</nlm:affiliation></affiliation></author><author><name sortKey="Qin, Kun" sort="Qin, Kun" uniqKey="Qin K" first="Kun" last="Qin">Kun Qin</name><affiliation><nlm:affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</nlm:affiliation></affiliation></author><author><name sortKey="Tan, Wenjie" sort="Tan, Wenjie" uniqKey="Tan W" first="Wenjie" last="Tan">Wenjie Tan</name><affiliation><nlm:affiliation>School of Laboratory Medicine and Life Science and Key Laboratory of Medical Virology, Wenzhou 325035, China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Emerging microbes & infections</title><idno type="eISSN">2222-1751</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (immunology)</term><term>Antibodies, Neutralizing (immunology)</term><term>Antibodies, Viral (immunology)</term><term>Chlorocebus aethiops</term><term>Coronavirus Infections (therapy)</term><term>Humans</term><term>Immunization</term><term>Mice</term><term>Middle East Respiratory Syndrome Coronavirus (chemistry)</term><term>Middle East Respiratory Syndrome Coronavirus (immunology)</term><term>Models, Molecular</term><term>Protein Binding</term><term>Receptors, Virus</term><term>Spike Glycoprotein, Coronavirus (administration & dosage)</term><term>Spike Glycoprotein, Coronavirus (chemistry)</term><term>Spike Glycoprotein, Coronavirus (genetics)</term><term>Spike Glycoprotein, Coronavirus (immunology)</term><term>Vero Cells</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Neutralizing</term><term>Antibodies, Viral</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="therapy" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Humans</term><term>Immunization</term><term>Mice</term><term>Models, Molecular</term><term>Protein Binding</term><term>Receptors, Virus</term><term>Vero Cells</term><term>Viral Vaccines</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Middle East respiratory syndrome coronavirus (MERS-CoV) has caused fatal infections, some through hospital-acquired transmission, in affected regions since its emergence in 2012. Although the virus is not pandemic among humans, it poses a great threat to public health due to its zoonotic origin. Thus, both preventative and therapeutic countermeasures are urgently needed. In this study, we discovered a panel of neutralizing monoclonal antibodies (mAbs) against MERS-CoV, which mapped to a wide range of regions on the spike (S) protein of the virus. In addition to mAbs with neutralizing epitopes located on the receptor-binding domain, one mAb, 5F9, which binds to the N-terminal domain (NTD) of the MERS-CoV S1 subunit, showed efficient neutralizing activity against the wild-type MERS-CoV strain EMC/2012, with a half maximal inhibitory concentration of 0.2 μg/mL. We concluded that a novel neutralizing epitope for MERS-CoV also resides on the NTD of the S protein, indicating that the NTD might be important during the viral infection process. Our findings have significant implications for further vaccine design and for the development of prophylactic and therapeutic monoclonal immunotherapies against MERS-CoV infection.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28536429</PMID><DateCompleted><Year>2017</Year><Month>12</Month><Day>18</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2222-1751</ISSN><JournalIssue CitedMedium="Internet"><Volume>6</Volume><Issue>5</Issue><PubDate><Year>2017</Year><Month>May</Month><Day>24</Day></PubDate></JournalIssue><Title>Emerging microbes & infections</Title><ISOAbbreviation>Emerg Microbes Infect</ISOAbbreviation></Journal><ArticleTitle>A novel neutralizing monoclonal antibody targeting the N-terminal domain of the MERS-CoV spike protein.</ArticleTitle><Pagination><MedlinePgn>e37</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/emi.2017.18</ELocationID><Abstract><AbstractText>Middle East respiratory syndrome coronavirus (MERS-CoV) has caused fatal infections, some through hospital-acquired transmission, in affected regions since its emergence in 2012. Although the virus is not pandemic among humans, it poses a great threat to public health due to its zoonotic origin. Thus, both preventative and therapeutic countermeasures are urgently needed. In this study, we discovered a panel of neutralizing monoclonal antibodies (mAbs) against MERS-CoV, which mapped to a wide range of regions on the spike (S) protein of the virus. In addition to mAbs with neutralizing epitopes located on the receptor-binding domain, one mAb, 5F9, which binds to the N-terminal domain (NTD) of the MERS-CoV S1 subunit, showed efficient neutralizing activity against the wild-type MERS-CoV strain EMC/2012, with a half maximal inhibitory concentration of 0.2 μg/mL. We concluded that a novel neutralizing epitope for MERS-CoV also resides on the NTD of the S protein, indicating that the NTD might be important during the viral infection process. Our findings have significant implications for further vaccine design and for the development of prophylactic and therapeutic monoclonal immunotherapies against MERS-CoV infection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Yingzhu</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>School of Laboratory Medicine and Life Science and Key Laboratory of Medical Virology, Wenzhou 325035, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Shuai</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jia</LastName><ForeName>Hao</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>School of Laboratory Medicine and Life Science and Key Laboratory of Medical Virology, Wenzhou 325035, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Deng</LastName><ForeName>Yao</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Jianfang</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Baoying</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Yueyang</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lan</LastName><ForeName>Jiaming</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Wenling</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lou</LastName><ForeName>Yongliang</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>School of Laboratory Medicine and Life Science and Key Laboratory of Medical Virology, Wenzhou 325035, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qin</LastName><ForeName>Kun</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tan</LastName><ForeName>Wenjie</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>School of Laboratory Medicine and Life Science and Key Laboratory of Medical Virology, Wenzhou 325035, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>05</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Emerg Microbes Infect</MedlineTA><NlmUniqueID>101594885</NlmUniqueID><ISSNLinking>2222-1751</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D057134">Antibodies, Neutralizing</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011991">Receptors, Virus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D064370">Spike Glycoprotein, 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Sci U S A. 2011 Jun 28;108(26):10696-701</Citation><ArticleIdList><ArticleId IdType="pubmed">21670291</ArticleId></ArticleIdList></Reference><Reference><Citation>J Formos Med Assoc. 2013 Jul;112(7):372-81</Citation><ArticleIdList><ArticleId IdType="pubmed">23883791</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2014 Jun;88(12):7045-53</Citation><ArticleIdList><ArticleId IdType="pubmed">24719424</ArticleId></ArticleIdList></Reference><Reference><Citation>Vaccine. 2014 Oct 21;32(46):6170-6176</Citation><ArticleIdList><ArticleId IdType="pubmed">25240756</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2013 Sep;87(17):9939-42</Citation><ArticleIdList><ArticleId IdType="pubmed">23824801</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2016 Mar 3;531(7592):118-21</Citation><ArticleIdList><ArticleId IdType="pubmed">26935699</ArticleId></ArticleIdList></Reference><Reference><Citation>Vaccine. 2016 Jun 3;34(26):2982-7</Citation><ArticleIdList><ArticleId IdType="pubmed">27083424</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Res. 2013 Aug;23(8):986-93</Citation><ArticleIdList><ArticleId IdType="pubmed">23835475</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2012 Nov 8;367 (19):1814-20</Citation><ArticleIdList><ArticleId IdType="pubmed">23075143</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1996 Aug;70(8):5634-7</Citation><ArticleIdList><ArticleId IdType="pubmed">8764078</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Commun. 2016 May 10;7:11544</Citation><ArticleIdList><ArticleId IdType="pubmed">27161536</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2013 Oct;87(19):10777-83</Citation><ArticleIdList><ArticleId IdType="pubmed">23903833</ArticleId></ArticleIdList></Reference><Reference><Citation>J Infect. 2013 Aug;67(2):156-8</Citation><ArticleIdList><ArticleId IdType="pubmed">23603488</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Transl Med. 2014 Apr 30;6(234):234ra59</Citation><ArticleIdList><ArticleId IdType="pubmed">24778414</ArticleId></ArticleIdList></Reference><Reference><Citation>EBioMedicine. 2015 Aug 18;2(10):1438-46</Citation><ArticleIdList><ArticleId IdType="pubmed">26629538</ArticleId></ArticleIdList></Reference><Reference><Citation>Vaccine. 2014 Apr 11;32(18):2100-8</Citation><ArticleIdList><ArticleId IdType="pubmed">24560617</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2013 Aug 8;500(7461):227-31</Citation><ArticleIdList><ArticleId IdType="pubmed">23831647</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2014 May 13;111(19):E2018-26</Citation><ArticleIdList><ArticleId IdType="pubmed">24778221</ArticleId></ArticleIdList></Reference><Reference><Citation>Immunity. 2014 May 15;40(5):669-80</Citation><ArticleIdList><ArticleId IdType="pubmed">24768348</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Res. 2017 Jan;27(1):119-129</Citation><ArticleIdList><ArticleId IdType="pubmed">28008928</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Commun. 2015 Jul 28;6:7712</Citation><ArticleIdList><ArticleId IdType="pubmed">26218507</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2014 Jul;88(14):7796-805</Citation><ArticleIdList><ArticleId IdType="pubmed">24789777</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol. 2005 Apr 15;174(8):4908-15</Citation><ArticleIdList><ArticleId IdType="pubmed">15814718</ArticleId></ArticleIdList></Reference><Reference><Citation>Hum Vaccin Immunother. 2015;11(5):1244-50</Citation><ArticleIdList><ArticleId IdType="pubmed">25874632</ArticleId></ArticleIdList></Reference><Reference><Citation>Epidemiol Health. 2015 Jul 21;37:e2015033</Citation><ArticleIdList><ArticleId IdType="pubmed">26212508</ArticleId></ArticleIdList></Reference><Reference><Citation>Expert Opin Biol Ther. 2015 ;15(11):1647-51</Citation><ArticleIdList><ArticleId IdType="pubmed">26414077</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Res. 2015 Nov;25(11):1237-49</Citation><ArticleIdList><ArticleId IdType="pubmed">26391698</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2013 Aug 29;369(9):884-6</Citation><ArticleIdList><ArticleId IdType="pubmed">23923992</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Infect Dis. 2016 Mar 15;62(6):755-60</Citation><ArticleIdList><ArticleId IdType="pubmed">26679623</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2013 Mar 14;495(7440):251-4</Citation><ArticleIdList><ArticleId IdType="pubmed">23486063</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Microbiol Rev. 2015 Apr;28(2):465-522</Citation><ArticleIdList><ArticleId IdType="pubmed">25810418</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 2015 Apr 16;202:151-9</Citation><ArticleIdList><ArticleId IdType="pubmed">25445336</ArticleId></ArticleIdList></Reference><Reference><Citation>Emerg Infect Dis. 2013 May;19(5):736-42B</Citation><ArticleIdList><ArticleId IdType="pubmed">23693015</ArticleId></ArticleIdList></Reference><Reference><Citation>J Infect. 2012 Dec;65(6):477-89</Citation><ArticleIdList><ArticleId IdType="pubmed">23072791</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2013 Aug 1;369(5):407-16</Citation><ArticleIdList><ArticleId IdType="pubmed">23782161</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet Infect Dis. 2014 Feb;14 (2):140-5</Citation><ArticleIdList><ArticleId IdType="pubmed">24355866</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2015 Jul 14;112(28):8738-43</Citation><ArticleIdList><ArticleId IdType="pubmed">26124093</ArticleId></ArticleIdList></Reference><Reference><Citation>Viruses. 2016 Feb 23;8(3):55</Citation><ArticleIdList><ArticleId IdType="pubmed">26907329</ArticleId></ArticleIdList></Reference><Reference><Citation>Euro Surveill. 2013 Mar 14;18(11):20427</Citation><ArticleIdList><ArticleId IdType="pubmed">23517868</ArticleId></ArticleIdList></Reference><Reference><Citation>MBio. 2014 Apr 29;5(3):e01146-14</Citation><ArticleIdList><ArticleId IdType="pubmed">24781747</ArticleId></ArticleIdList></Reference><Reference><Citation>MBio. 2012 Nov 20;3(6):null</Citation><ArticleIdList><ArticleId IdType="pubmed">23170002</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2014 Aug 26;111(34):12516-21</Citation><ArticleIdList><ArticleId IdType="pubmed">25114257</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2014 Oct 21;111(42):15214-9</Citation><ArticleIdList><ArticleId IdType="pubmed">25288733</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2013 Dec 04;8(12):e81587</Citation><ArticleIdList><ArticleId IdType="pubmed">24324708</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Infect Dis. 2015 Sep;38:65-7</Citation><ArticleIdList><ArticleId IdType="pubmed">26216766</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2013 Aug;87(16):9379-83</Citation><ArticleIdList><ArticleId IdType="pubmed">23785207</ArticleId></ArticleIdList></Reference><Reference><Citation>J Infect. 2013 May;66(5):464-6</Citation><ArticleIdList><ArticleId IdType="pubmed">23266463</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2015 Aug 18;112(33):10473-8</Citation><ArticleIdList><ArticleId IdType="pubmed">26216974</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Genes. 2015 Jun;50(3):509-13</Citation><ArticleIdList><ArticleId 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