MersV1, PubMed, Corpus, bibRecord, 000B54

Inclusion of MERS-spike protein ELISA in algorithm to determine serologic evidence of MERS-CoV infection.

Identifieur interne : 000B54 ( PubMed/Corpus ); précédent : 000B53; suivant : 000B55

Inclusion of MERS-spike protein ELISA in algorithm to determine serologic evidence of MERS-CoV infection.

Auteurs : Suvang Trivedi ; Congrong Miao ; Mohammad M. Al-Abdallat ; Aktham Haddadin ; Sultan Alqasrawi ; Ibrahim Iblan ; Mohannad A. Nsour ; Tarek Alsanouri ; Sami S. Ali ; Brian Rha ; Susan I. Gerber ; Daniel C. Payne ; Azaibi Tamin ; Natalie J. Thornburg

Source :

Journal of medical virology [ 1096-9071 ] ; 2018.

RBID : pubmed:28906003

English descriptors

KwdEn :
- Algorithms, Antibodies, Viral (blood), Coronavirus Infections (diagnosis), Enzyme-Linked Immunosorbent Assay (methods), Humans, Middle East Respiratory Syndrome Coronavirus (immunology), Serologic Tests (methods), Spike Glycoprotein, Coronavirus (immunology).
MESH :
- chemical , blood : Antibodies, Viral.
- diagnosis : Coronavirus Infections.
- immunology : Middle East Respiratory Syndrome Coronavirus, Spike Glycoprotein, Coronavirus.
- methods : Enzyme-Linked Immunosorbent Assay, Serologic Tests.
- Algorithms, Humans.

Abstract

The Centers for Disease Control and Prevention (CDC) algorithm for detecting presence of serum antibodies against Middle East Respiratory Syndrome coronavirus (MERS-CoV) in subjects with potential infections with the virus has included screening by indirect ELISA against recombinant nucleocapsid (N) protein and confirmation by immunofluorescent staining of infected monolayers and/or microneutralization titration. Other international groups include indirect ELISA assays using the spike (S) protein, as part of their serological determinations. In the current study, we describe development and validation of an indirect MERS-CoV S ELISA to be used as part of our serological determination for evidence of previous exposure to the virus.

DOI: 10.1002/jmv.24948
PubMed: 28906003

Links to Exploration step

pubmed:28906003

Le document en format XML

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<front><div type="abstract" xml:lang="en">The Centers for Disease Control and Prevention (CDC) algorithm for detecting presence of serum antibodies against Middle East Respiratory Syndrome coronavirus (MERS-CoV) in subjects with potential infections with the virus has included screening by indirect ELISA against recombinant nucleocapsid (N) protein and confirmation by immunofluorescent staining of infected monolayers and/or microneutralization titration. Other international groups include indirect ELISA assays using the spike (S) protein, as part of their serological determinations. In the current study, we describe development and validation of an indirect MERS-CoV S ELISA to be used as part of our serological determination for evidence of previous exposure to the virus.</div>
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   |texte=   Inclusion of MERS-spike protein ELISA in algorithm to determine serologic evidence of MERS-CoV infection.
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Inclusion of MERS-spike protein ELISA in algorithm to determine serologic evidence of MERS-CoV infection.

Inclusion of MERS-spike protein ELISA in algorithm to determine serologic evidence of MERS-CoV infection.

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