Porcine epidemic diarrhea virus papain-like protease 2 can be noncompetitively inhibited by 6-thioguanine.
Identifieur interne : 000802 ( PubMed/Corpus ); précédent : 000801; suivant : 000803Porcine epidemic diarrhea virus papain-like protease 2 can be noncompetitively inhibited by 6-thioguanine.
Auteurs : Hsu-Feng Chu ; Chiao-Che Chen ; David C. Moses ; Yau-Hung Chen ; Chao-Hsiung Lin ; Ying-Chieh Tsai ; Chi-Yuan ChouSource :
- Antiviral research [ 1872-9096 ] ; 2018.
English descriptors
- KwdEn :
- Antiviral Agents (pharmacology), Binding Sites (drug effects), Coronavirus (drug effects), Coronavirus Infections, Kinetics, Molecular Docking Simulation, Papain (antagonists & inhibitors), Papain (chemistry), Papain (genetics), Papain (isolation & purification), Porcine epidemic diarrhea virus (drug effects), Porcine epidemic diarrhea virus (genetics), Protein Conformation (drug effects), Recombinant Proteins, Thioguanine (pharmacology).
- MESH :
- chemical , antagonists & inhibitors : Papain.
- chemical , chemistry : Papain.
- chemical , genetics : Papain.
- chemical , isolation & purification : Papain.
- chemical , pharmacology : Antiviral Agents, Thioguanine.
- drug effects : Binding Sites, Coronavirus, Porcine epidemic diarrhea virus, Protein Conformation.
- genetics : Porcine epidemic diarrhea virus.
- Coronavirus Infections, Kinetics, Molecular Docking Simulation, Recombinant Proteins.
Abstract
Porcine epidemic diarrhea virus (PEDV) is a coronavirus (CoV) discovered in the 1970s that infects the intestinal tract of pigs, resulting in diarrhea and vomiting. It can cause extreme dehydration and death in neonatal piglets. In Asia, modified live attenuated vaccines have been used to control PEDV infection in recent years. However, a new strain of PEDV that belongs to genogroup 2a appeared in the USA in 2013 and then quickly spread to Canada and Mexico as well as Asian and European countries. Due to the less effective protective immunity provided by the vaccines against this new strain, it has caused considerable agricultural and economic loss worldwide. The emergence of this new strain increases the importance of understanding PEDV as well as strategies for inhibiting it. Coronaviral proteases, including main proteases and papain-like proteases, are ideal antiviral targets because of their essential roles in viral maturation. Here we provide a first description of the expression, purification and structural characteristics of recombinant PEDV papain-like protease 2, moreover present our finding that 6-thioguanine, a chemotherapeutic drug, in contrast to its competitive inhibition on SARS- and MERS-CoV papain-like proteases, is a noncompetitive inhibitor of PEDV papain-like protease 2.
DOI: 10.1016/j.antiviral.2018.08.011
PubMed: 30138642
Links to Exploration step
pubmed:30138642Le document en format XML
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Electronic address: cychou@ym.edu.tw.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Antiviral research</title><idno type="eISSN">1872-9096</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antiviral Agents (pharmacology)</term><term>Binding Sites (drug effects)</term><term>Coronavirus (drug effects)</term><term>Coronavirus Infections</term><term>Kinetics</term><term>Molecular Docking Simulation</term><term>Papain (antagonists & inhibitors)</term><term>Papain (chemistry)</term><term>Papain (genetics)</term><term>Papain (isolation & purification)</term><term>Porcine epidemic diarrhea virus (drug effects)</term><term>Porcine epidemic diarrhea virus (genetics)</term><term>Protein Conformation (drug effects)</term><term>Recombinant Proteins</term><term>Thioguanine (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Papain</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Papain</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Papain</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Papain</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term><term>Thioguanine</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Binding Sites</term><term>Coronavirus</term><term>Porcine epidemic diarrhea virus</term><term>Protein Conformation</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Porcine epidemic diarrhea virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Coronavirus Infections</term><term>Kinetics</term><term>Molecular Docking Simulation</term><term>Recombinant Proteins</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Porcine epidemic diarrhea virus (PEDV) is a coronavirus (CoV) discovered in the 1970s that infects the intestinal tract of pigs, resulting in diarrhea and vomiting. It can cause extreme dehydration and death in neonatal piglets. In Asia, modified live attenuated vaccines have been used to control PEDV infection in recent years. However, a new strain of PEDV that belongs to genogroup 2a appeared in the USA in 2013 and then quickly spread to Canada and Mexico as well as Asian and European countries. Due to the less effective protective immunity provided by the vaccines against this new strain, it has caused considerable agricultural and economic loss worldwide. The emergence of this new strain increases the importance of understanding PEDV as well as strategies for inhibiting it. Coronaviral proteases, including main proteases and papain-like proteases, are ideal antiviral targets because of their essential roles in viral maturation. Here we provide a first description of the expression, purification and structural characteristics of recombinant PEDV papain-like protease 2, moreover present our finding that 6-thioguanine, a chemotherapeutic drug, in contrast to its competitive inhibition on SARS- and MERS-CoV papain-like proteases, is a noncompetitive inhibitor of PEDV papain-like protease 2.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30138642</PMID><DateCompleted><Year>2019</Year><Month>10</Month><Day>03</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>07</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1872-9096</ISSN><JournalIssue CitedMedium="Internet"><Volume>158</Volume><PubDate><Year>2018</Year><Month>10</Month></PubDate></JournalIssue><Title>Antiviral research</Title><ISOAbbreviation>Antiviral Res.</ISOAbbreviation></Journal><ArticleTitle>Porcine epidemic diarrhea virus papain-like protease 2 can be noncompetitively inhibited by 6-thioguanine.</ArticleTitle><Pagination><MedlinePgn>199-205</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0166-3542(18)30218-3</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.antiviral.2018.08.011</ELocationID><Abstract><AbstractText>Porcine epidemic diarrhea virus (PEDV) is a coronavirus (CoV) discovered in the 1970s that infects the intestinal tract of pigs, resulting in diarrhea and vomiting. It can cause extreme dehydration and death in neonatal piglets. In Asia, modified live attenuated vaccines have been used to control PEDV infection in recent years. However, a new strain of PEDV that belongs to genogroup 2a appeared in the USA in 2013 and then quickly spread to Canada and Mexico as well as Asian and European countries. Due to the less effective protective immunity provided by the vaccines against this new strain, it has caused considerable agricultural and economic loss worldwide. The emergence of this new strain increases the importance of understanding PEDV as well as strategies for inhibiting it. Coronaviral proteases, including main proteases and papain-like proteases, are ideal antiviral targets because of their essential roles in viral maturation. Here we provide a first description of the expression, purification and structural characteristics of recombinant PEDV papain-like protease 2, moreover present our finding that 6-thioguanine, a chemotherapeutic drug, in contrast to its competitive inhibition on SARS- and MERS-CoV papain-like proteases, is a noncompetitive inhibitor of PEDV papain-like protease 2.</AbstractText><CopyrightInformation>Copyright © 2018 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chu</LastName><ForeName>Hsu-Feng</ForeName><Initials>HF</Initials><AffiliationInfo><Affiliation>Biomedical Industry Ph.D. Program, National Yang-Ming University, Taipei 112, Taiwan; Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Taipei 112, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Chiao-Che</ForeName><Initials>CC</Initials><AffiliationInfo><Affiliation>Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Taipei 112, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Moses</LastName><ForeName>David C</ForeName><Initials>DC</Initials><AffiliationInfo><Affiliation>Department of Chemistry, Tamkang University, Tamsui 251, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Yau-Hung</ForeName><Initials>YH</Initials><AffiliationInfo><Affiliation>Department of Chemistry, Tamkang University, Tamsui 251, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Chao-Hsiung</ForeName><Initials>CH</Initials><AffiliationInfo><Affiliation>Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Taipei 112, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tsai</LastName><ForeName>Ying-Chieh</ForeName><Initials>YC</Initials><AffiliationInfo><Affiliation>Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei 112, Taiwan; Probiotic Research Center, National Yang-Ming University, Taipei 112, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chou</LastName><ForeName>Chi-Yuan</ForeName><Initials>CY</Initials><AffiliationInfo><Affiliation>Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Taipei 112, Taiwan. 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MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D062105" MajorTopicYN="N">Molecular Docking Simulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010206" MajorTopicYN="N">Papain</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="Y">antagonists & inhibitors</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053485" MajorTopicYN="N">Porcine epidemic diarrhea virus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011487" MajorTopicYN="N">Protein Conformation</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug 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