MersV1, PubMed, Corpus, bibRecord, 000665

Development and validation of different indirect ELISAs for MERS-CoV serological testing.

Identifieur interne : 000665 ( PubMed/Corpus ); précédent : 000664; suivant : 000666

Development and validation of different indirect ELISAs for MERS-CoV serological testing.

Auteurs : Anwar M. Hashem ; Sawsan S. Al-Amri ; Tagreed L. Al-Subhi ; Loai A. Siddiq ; Ahmed M. Hassan ; Maha M. Alawi ; Rowa Y. Alhabbab ; Salwa I. Hindawi ; Osama B. Mohammed ; Nabil S. Amor ; Abdulaziz N. Alagaili ; Ahmed A. Mirza ; Esam I. Azhar

Source :

Journal of immunological methods [ 1872-7905 ] ; 2019.

RBID : pubmed:30659836

English descriptors

KwdEn :
- Animals, Antibodies, Viral (blood), Antibodies, Viral (immunology), Chlorocebus aethiops, Enzyme-Linked Immunosorbent Assay, Humans, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (immunology), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Recombinant Proteins (blood), Recombinant Proteins (immunology), Serologic Tests, Vero Cells.
MESH :
- chemical , blood : Antibodies, Viral, Recombinant Proteins.
- chemical , immunology : Antibodies, Viral, Recombinant Proteins.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- immunology : Middle East Respiratory Syndrome Coronavirus.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- Animals, Chlorocebus aethiops, Enzyme-Linked Immunosorbent Assay, Humans, Serologic Tests, Vero Cells.

Abstract

Since 2012, MERS-CoV has caused up to 2220 cases and 790 deaths in 27 countries with Saudi Arabia being the most affected country with ~83.1% of the cases and ~38.8% local death rate. Current serological assays such as microneutralization (MN), plaque reduction neutralization, immunofluorescence, protein microarray or pseudoparticle neutralization assays rely on handling of live MERS-CoV in high containment laboratories or need for expensive and special equipment and reagents and highly trained personnel which represent a technical hurdle for most laboratories in resource-limited MERS-CoV endemic countries. Here, we developed, compared and evaluated three different indirect ELISAs based on MERS-CoV nucleocapsid protein (N), spike (S) ectodomain (amino acids 1-1297) and S1 subunit (amino acids 1-725) and compared them with MN assay. The developed ELISAs were evaluated using large number of confirmed seropositive (79 samples) and seronegative (274 samples) MERS-CoV human serum samples. Both rS1- and rS-ELISAs maintained high sensitivity and specificity (≥90%) across a wider range of OD values compared to rN-ELISA. Moreover, rS1- and rS-based ELISAs showed better agreement and correlation with MN assay in contrast to rN-ELISA. Collectively, our data demonstrate that rS1-ELISA and rS-ELISA are more reliable than rN-ELISA and represent a suitable choice for seroepidemiological testing and surveillance in MERS-CoV endemic regions.

DOI: 10.1016/j.jim.2019.01.005
PubMed: 30659836

Links to Exploration step

pubmed:30659836

Le document en format XML

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<front><div type="abstract" xml:lang="en">Since 2012, MERS-CoV has caused up to 2220 cases and 790 deaths in 27 countries with Saudi Arabia being the most affected country with ~83.1% of the cases and ~38.8% local death rate. Current serological assays such as microneutralization (MN), plaque reduction neutralization, immunofluorescence, protein microarray or pseudoparticle neutralization assays rely on handling of live MERS-CoV in high containment laboratories or need for expensive and special equipment and reagents and highly trained personnel which represent a technical hurdle for most laboratories in resource-limited MERS-CoV endemic countries. Here, we developed, compared and evaluated three different indirect ELISAs based on MERS-CoV nucleocapsid protein (N), spike (S) ectodomain (amino acids 1-1297) and S1 subunit (amino acids 1-725) and compared them with MN assay. The developed ELISAs were evaluated using large number of confirmed seropositive (79 samples) and seronegative (274 samples) MERS-CoV human serum samples. Both rS1- and rS-ELISAs maintained high sensitivity and specificity (≥90%) across a wider range of OD values compared to rN-ELISA. Moreover, rS1- and rS-based ELISAs showed better agreement and correlation with MN assay in contrast to rN-ELISA. Collectively, our data demonstrate that rS1-ELISA and rS-ELISA are more reliable than rN-ELISA and represent a suitable choice for seroepidemiological testing and surveillance in MERS-CoV endemic regions.</div>
</front>
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<Abstract><AbstractText>Since 2012, MERS-CoV has caused up to 2220 cases and 790 deaths in 27 countries with Saudi Arabia being the most affected country with ~83.1% of the cases and ~38.8% local death rate. Current serological assays such as microneutralization (MN), plaque reduction neutralization, immunofluorescence, protein microarray or pseudoparticle neutralization assays rely on handling of live MERS-CoV in high containment laboratories or need for expensive and special equipment and reagents and highly trained personnel which represent a technical hurdle for most laboratories in resource-limited MERS-CoV endemic countries. Here, we developed, compared and evaluated three different indirect ELISAs based on MERS-CoV nucleocapsid protein (N), spike (S) ectodomain (amino acids 1-1297) and S1 subunit (amino acids 1-725) and compared them with MN assay. The developed ELISAs were evaluated using large number of confirmed seropositive (79 samples) and seronegative (274 samples) MERS-CoV human serum samples. Both rS1- and rS-ELISAs maintained high sensitivity and specificity (≥90%) across a wider range of OD values compared to rN-ELISA. Moreover, rS1- and rS-based ELISAs showed better agreement and correlation with MN assay in contrast to rN-ELISA. Collectively, our data demonstrate that rS1-ELISA and rS-ELISA are more reliable than rN-ELISA and represent a suitable choice for seroepidemiological testing and surveillance in MERS-CoV endemic regions.</AbstractText>
<CopyrightInformation>Copyright © 2019 Elsevier B.V. All rights reserved.</CopyrightInformation>
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<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hashem</LastName>
<ForeName>Anwar M</ForeName>
<Initials>AM</Initials>
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<Author ValidYN="Y"><LastName>Al-Amri</LastName>
<ForeName>Sawsan S</ForeName>
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<ForeName>Tagreed L</ForeName>
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<Author ValidYN="Y"><LastName>Hassan</LastName>
<ForeName>Ahmed M</ForeName>
<Initials>AM</Initials>
<AffiliationInfo><Affiliation>Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.</Affiliation>
</AffiliationInfo>
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</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Alhabbab</LastName>
<ForeName>Rowa Y</ForeName>
<Initials>RY</Initials>
<AffiliationInfo><Affiliation>Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.</Affiliation>
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<ForeName>Osama B</ForeName>
<Initials>OB</Initials>
<AffiliationInfo><Affiliation>KSU Mammals Research Chair, Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.</Affiliation>
</AffiliationInfo>
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<ForeName>Nabil S</ForeName>
<Initials>NS</Initials>
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<ForeName>Ahmed A</ForeName>
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<AffiliationInfo><Affiliation>Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Azhar</LastName>
<ForeName>Esam I</ForeName>
<Initials>EI</Initials>
<AffiliationInfo><Affiliation>Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.</Affiliation>
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<Month>01</Month>
<Day>16</Day>
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<MedlineJournalInfo><Country>Netherlands</Country>
<MedlineTA>J Immunol Methods</MedlineTA>
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Development and validation of different indirect ELISAs for MERS-CoV serological testing.

Development and validation of different indirect ELISAs for MERS-CoV serological testing.

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