Isolation and growth characterization of novel full length and deletion mutant human MERS-CoV strains from clinical specimens collected during 2015.
Identifieur interne : 000400 ( PubMed/Corpus ); précédent : 000399; suivant : 000401Isolation and growth characterization of novel full length and deletion mutant human MERS-CoV strains from clinical specimens collected during 2015.
Auteurs : Azaibi Tamin ; Krista Queen ; Clinton R. Paden ; Xiaoyan Lu ; Erica Andres ; Senthilkumar K. Sakthivel ; Yan Li ; Ying Tao ; Jing Zhang ; Shifaq Kamili ; Abdullah M. Assiri ; Ali Alshareef ; Taghreed A. Alaifan ; Asmaa M. Altamimi ; Hani Jokhdar ; John T. Watson ; Susan I. Gerber ; Suxiang Tong ; Natalie J. ThornburgSource :
- The Journal of general virology [ 1465-2099 ] ; 2019.
Abstract
Middle East respiratory syndrome (MERS) is a viral respiratory illness first reported in Saudi Arabia in September 2012 caused by the human coronavirus (CoV), MERS-CoV. Using full-genome sequencing and phylogenetic analysis, scientists have identified three clades and multiple lineages of MERS-CoV in humans and the zoonotic host, dromedary camels. In this study, we have characterized eight MERS-CoV isolates collected from patients in Saudi Arabia in 2015. We have performed full-genome sequencing on the viral isolates, and compared them to the corresponding clinical specimens. All isolates were clade B, lineages 4 and 5. Three of the isolates carry deletions located on three independent regions of the genome in the 5'UTR, ORF1a and ORF3. All novel MERS-CoV strains replicated efficiently in Vero and Huh7 cells. Viruses with deletions in the 5'UTR and ORF1a exhibited impaired viral release in Vero cells. These data emphasize the plasticity of the MERS-CoV genome during human infection.
DOI: 10.1099/jgv.0.001334
PubMed: 31592752
Links to Exploration step
pubmed:31592752Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Isolation and growth characterization of novel full length and deletion mutant human MERS-CoV strains from clinical specimens collected during 2015.</title><author><name sortKey="Tamin, Azaibi" sort="Tamin, Azaibi" uniqKey="Tamin A" first="Azaibi" last="Tamin">Azaibi Tamin</name><affiliation><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Queen, Krista" sort="Queen, Krista" uniqKey="Queen K" first="Krista" last="Queen">Krista Queen</name><affiliation><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Paden, Clinton R" sort="Paden, Clinton R" uniqKey="Paden C" first="Clinton R" last="Paden">Clinton R. Paden</name><affiliation><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Lu, Xiaoyan" sort="Lu, Xiaoyan" uniqKey="Lu X" first="Xiaoyan" last="Lu">Xiaoyan Lu</name><affiliation><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Andres, Erica" sort="Andres, Erica" uniqKey="Andres E" first="Erica" last="Andres">Erica Andres</name><affiliation><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Sakthivel, Senthilkumar K" sort="Sakthivel, Senthilkumar K" uniqKey="Sakthivel S" first="Senthilkumar K" last="Sakthivel">Senthilkumar K. Sakthivel</name><affiliation><nlm:affiliation>Batelle, Columbus, OH, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name><affiliation><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Tao, Ying" sort="Tao, Ying" uniqKey="Tao Y" first="Ying" last="Tao">Ying Tao</name><affiliation><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Jing" sort="Zhang, Jing" uniqKey="Zhang J" first="Jing" last="Zhang">Jing Zhang</name><affiliation><nlm:affiliation>IHRC, Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Kamili, Shifaq" sort="Kamili, Shifaq" uniqKey="Kamili S" first="Shifaq" last="Kamili">Shifaq Kamili</name><affiliation><nlm:affiliation>IHRC, Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Assiri, Abdullah M" sort="Assiri, Abdullah M" uniqKey="Assiri A" first="Abdullah M" last="Assiri">Abdullah M. Assiri</name><affiliation><nlm:affiliation>Kingdom of Saudi Arabia Ministry of Health, Riyadh, Saudi Arabia.</nlm:affiliation></affiliation></author><author><name sortKey="Alshareef, Ali" sort="Alshareef, Ali" uniqKey="Alshareef A" first="Ali" last="Alshareef">Ali Alshareef</name><affiliation><nlm:affiliation>Kingdom of Saudi Arabia Ministry of Health, Riyadh, Saudi Arabia.</nlm:affiliation></affiliation></author><author><name sortKey="Alaifan, Taghreed A" sort="Alaifan, Taghreed A" uniqKey="Alaifan T" first="Taghreed A" last="Alaifan">Taghreed A. Alaifan</name><affiliation><nlm:affiliation>Kingdom of Saudi Arabia Ministry of Health, Riyadh, Saudi Arabia.</nlm:affiliation></affiliation></author><author><name sortKey="Altamimi, Asmaa M" sort="Altamimi, Asmaa M" uniqKey="Altamimi A" first="Asmaa M" last="Altamimi">Asmaa M. Altamimi</name><affiliation><nlm:affiliation>Kingdom of Saudi Arabia Ministry of Health, Riyadh, Saudi Arabia.</nlm:affiliation></affiliation></author><author><name sortKey="Jokhdar, Hani" sort="Jokhdar, Hani" uniqKey="Jokhdar H" first="Hani" last="Jokhdar">Hani Jokhdar</name><affiliation><nlm:affiliation>Kingdom of Saudi Arabia Ministry of Health, Riyadh, Saudi Arabia.</nlm:affiliation></affiliation></author><author><name sortKey="Watson, John T" sort="Watson, John T" uniqKey="Watson J" first="John T" last="Watson">John T. Watson</name><affiliation><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Gerber, Susan I" sort="Gerber, Susan I" uniqKey="Gerber S" first="Susan I" last="Gerber">Susan I. Gerber</name><affiliation><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Tong, Suxiang" sort="Tong, Suxiang" uniqKey="Tong S" first="Suxiang" last="Tong">Suxiang Tong</name><affiliation><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Thornburg, Natalie J" sort="Thornburg, Natalie J" uniqKey="Thornburg N" first="Natalie J" last="Thornburg">Natalie J. Thornburg</name><affiliation><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31592752</idno><idno type="pmid">31592752</idno><idno type="doi">10.1099/jgv.0.001334</idno><idno type="wicri:Area/PubMed/Corpus">000400</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000400</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Isolation and growth characterization of novel full length and deletion mutant human MERS-CoV strains from clinical specimens collected during 2015.</title><author><name sortKey="Tamin, Azaibi" sort="Tamin, Azaibi" uniqKey="Tamin A" first="Azaibi" last="Tamin">Azaibi Tamin</name><affiliation><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Queen, Krista" sort="Queen, Krista" uniqKey="Queen K" first="Krista" last="Queen">Krista Queen</name><affiliation><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Paden, Clinton R" sort="Paden, Clinton R" uniqKey="Paden C" first="Clinton R" last="Paden">Clinton R. Paden</name><affiliation><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Lu, Xiaoyan" sort="Lu, Xiaoyan" uniqKey="Lu X" first="Xiaoyan" last="Lu">Xiaoyan Lu</name><affiliation><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Andres, Erica" sort="Andres, Erica" uniqKey="Andres E" first="Erica" last="Andres">Erica Andres</name><affiliation><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Sakthivel, Senthilkumar K" sort="Sakthivel, Senthilkumar K" uniqKey="Sakthivel S" first="Senthilkumar K" last="Sakthivel">Senthilkumar K. Sakthivel</name><affiliation><nlm:affiliation>Batelle, Columbus, OH, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name><affiliation><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Tao, Ying" sort="Tao, Ying" uniqKey="Tao Y" first="Ying" last="Tao">Ying Tao</name><affiliation><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Jing" sort="Zhang, Jing" uniqKey="Zhang J" first="Jing" last="Zhang">Jing Zhang</name><affiliation><nlm:affiliation>IHRC, Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Kamili, Shifaq" sort="Kamili, Shifaq" uniqKey="Kamili S" first="Shifaq" last="Kamili">Shifaq Kamili</name><affiliation><nlm:affiliation>IHRC, Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Assiri, Abdullah M" sort="Assiri, Abdullah M" uniqKey="Assiri A" first="Abdullah M" last="Assiri">Abdullah M. Assiri</name><affiliation><nlm:affiliation>Kingdom of Saudi Arabia Ministry of Health, Riyadh, Saudi Arabia.</nlm:affiliation></affiliation></author><author><name sortKey="Alshareef, Ali" sort="Alshareef, Ali" uniqKey="Alshareef A" first="Ali" last="Alshareef">Ali Alshareef</name><affiliation><nlm:affiliation>Kingdom of Saudi Arabia Ministry of Health, Riyadh, Saudi Arabia.</nlm:affiliation></affiliation></author><author><name sortKey="Alaifan, Taghreed A" sort="Alaifan, Taghreed A" uniqKey="Alaifan T" first="Taghreed A" last="Alaifan">Taghreed A. Alaifan</name><affiliation><nlm:affiliation>Kingdom of Saudi Arabia Ministry of Health, Riyadh, Saudi Arabia.</nlm:affiliation></affiliation></author><author><name sortKey="Altamimi, Asmaa M" sort="Altamimi, Asmaa M" uniqKey="Altamimi A" first="Asmaa M" last="Altamimi">Asmaa M. Altamimi</name><affiliation><nlm:affiliation>Kingdom of Saudi Arabia Ministry of Health, Riyadh, Saudi Arabia.</nlm:affiliation></affiliation></author><author><name sortKey="Jokhdar, Hani" sort="Jokhdar, Hani" uniqKey="Jokhdar H" first="Hani" last="Jokhdar">Hani Jokhdar</name><affiliation><nlm:affiliation>Kingdom of Saudi Arabia Ministry of Health, Riyadh, Saudi Arabia.</nlm:affiliation></affiliation></author><author><name sortKey="Watson, John T" sort="Watson, John T" uniqKey="Watson J" first="John T" last="Watson">John T. Watson</name><affiliation><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Gerber, Susan I" sort="Gerber, Susan I" uniqKey="Gerber S" first="Susan I" last="Gerber">Susan I. Gerber</name><affiliation><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Tong, Suxiang" sort="Tong, Suxiang" uniqKey="Tong S" first="Suxiang" last="Tong">Suxiang Tong</name><affiliation><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Thornburg, Natalie J" sort="Thornburg, Natalie J" uniqKey="Thornburg N" first="Natalie J" last="Thornburg">Natalie J. Thornburg</name><affiliation><nlm:affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</nlm:affiliation></affiliation></author></analytic><series><title level="j">The Journal of general virology</title><idno type="eISSN">1465-2099</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Middle East respiratory syndrome (MERS) is a viral respiratory illness first reported in Saudi Arabia in September 2012 caused by the human coronavirus (CoV), MERS-CoV. Using full-genome sequencing and phylogenetic analysis, scientists have identified three clades and multiple lineages of MERS-CoV in humans and the zoonotic host, dromedary camels. In this study, we have characterized eight MERS-CoV isolates collected from patients in Saudi Arabia in 2015. We have performed full-genome sequencing on the viral isolates, and compared them to the corresponding clinical specimens. All isolates were clade B, lineages 4 and 5. Three of the isolates carry deletions located on three independent regions of the genome in the 5'UTR, ORF1a and ORF3. All novel MERS-CoV strains replicated efficiently in Vero and Huh7 cells. Viruses with deletions in the 5'UTR and ORF1a exhibited impaired viral release in Vero cells. These data emphasize the plasticity of the MERS-CoV genome during human infection.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">31592752</PMID><DateRevised><Year>2020</Year><Month>03</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1465-2099</ISSN><JournalIssue CitedMedium="Internet"><Volume>100</Volume><Issue>11</Issue><PubDate><Year>2019</Year><Month>Nov</Month></PubDate></JournalIssue><Title>The Journal of general virology</Title><ISOAbbreviation>J. Gen. Virol.</ISOAbbreviation></Journal><ArticleTitle>Isolation and growth characterization of novel full length and deletion mutant human MERS-CoV strains from clinical specimens collected during 2015.</ArticleTitle><Pagination><MedlinePgn>1523-1529</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1099/jgv.0.001334</ELocationID><Abstract><AbstractText>Middle East respiratory syndrome (MERS) is a viral respiratory illness first reported in Saudi Arabia in September 2012 caused by the human coronavirus (CoV), MERS-CoV. Using full-genome sequencing and phylogenetic analysis, scientists have identified three clades and multiple lineages of MERS-CoV in humans and the zoonotic host, dromedary camels. In this study, we have characterized eight MERS-CoV isolates collected from patients in Saudi Arabia in 2015. We have performed full-genome sequencing on the viral isolates, and compared them to the corresponding clinical specimens. All isolates were clade B, lineages 4 and 5. Three of the isolates carry deletions located on three independent regions of the genome in the 5'UTR, ORF1a and ORF3. All novel MERS-CoV strains replicated efficiently in Vero and Huh7 cells. Viruses with deletions in the 5'UTR and ORF1a exhibited impaired viral release in Vero cells. These data emphasize the plasticity of the MERS-CoV genome during human infection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tamin</LastName><ForeName>Azaibi</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Queen</LastName><ForeName>Krista</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Paden</LastName><ForeName>Clinton R</ForeName><Initials>CR</Initials><AffiliationInfo><Affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Xiaoyan</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Andres</LastName><ForeName>Erica</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sakthivel</LastName><ForeName>Senthilkumar K</ForeName><Initials>SK</Initials><AffiliationInfo><Affiliation>Batelle, Columbus, OH, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yan</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tao</LastName><ForeName>Ying</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Jing</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>IHRC, Atlanta, GA, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kamili</LastName><ForeName>Shifaq</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>IHRC, Atlanta, GA, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Assiri</LastName><ForeName>Abdullah M</ForeName><Initials>AM</Initials><AffiliationInfo><Affiliation>Kingdom of Saudi Arabia Ministry of Health, Riyadh, Saudi Arabia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Alshareef</LastName><ForeName>Ali</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Kingdom of Saudi Arabia Ministry of Health, Riyadh, Saudi Arabia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Alaifan</LastName><ForeName>Taghreed A</ForeName><Initials>TA</Initials><AffiliationInfo><Affiliation>Kingdom of Saudi Arabia Ministry of Health, Riyadh, Saudi Arabia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Altamimi</LastName><ForeName>Asmaa M</ForeName><Initials>AM</Initials><AffiliationInfo><Affiliation>Kingdom of Saudi Arabia Ministry of Health, Riyadh, Saudi Arabia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jokhdar</LastName><ForeName>Hani</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Kingdom of Saudi Arabia Ministry of Health, Riyadh, Saudi Arabia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Watson</LastName><ForeName>John T</ForeName><Initials>JT</Initials><AffiliationInfo><Affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gerber</LastName><ForeName>Susan I</ForeName><Initials>SI</Initials><AffiliationInfo><Affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tong</LastName><ForeName>Suxiang</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Thornburg</LastName><ForeName>Natalie J</ForeName><Initials>NJ</Initials><AffiliationInfo><Affiliation>National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Gen Virol</MedlineTA><NlmUniqueID>0077340</NlmUniqueID><ISSNLinking>0022-1317</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">MERS-CoV</Keyword><Keyword MajorTopicYN="N">Middle East respiratory syndrome human coronavirus</Keyword><Keyword MajorTopicYN="N">clinical isolates</Keyword><Keyword MajorTopicYN="N">coronavirus</Keyword><Keyword MajorTopicYN="N">genomic sequences</Keyword><Keyword MajorTopicYN="N">growth kinetics</Keyword><Keyword MajorTopicYN="N">phylogeny</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>10</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>10</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>10</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31592752</ArticleId><ArticleId IdType="doi">10.1099/jgv.0.001334</ArticleId><ArticleId IdType="pmc">PMC7079693</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Genome Announc. 2015 Aug 13;3(4):</Citation><ArticleIdList><ArticleId IdType="pubmed">26272560</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>mBio. 2014 Feb 18;5(1):</Citation><ArticleIdList><ArticleId IdType="pubmed">24549846</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Arch Virol. 2017 Aug;162(8):2445-2449</Citation><ArticleIdList><ArticleId IdType="pubmed">28421366</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virus Res. 2015 Aug 3;206:120-33</Citation><ArticleIdList><ArticleId IdType="pubmed">25736566</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Infect Dis. 2016 Sep 1;214(5):712-21</Citation><ArticleIdList><ArticleId IdType="pubmed">27302191</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>mBio. 2017 Aug 22;8(4):</Citation><ArticleIdList><ArticleId IdType="pubmed">28830941</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):16157-62</Citation><ArticleIdList><ArticleId IdType="pubmed">24043791</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Med Virol. 2017 Mar;89(3):542-545</Citation><ArticleIdList><ArticleId IdType="pubmed">27486688</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virol J. 2016 Jun 03;13:87</Citation><ArticleIdList><ArticleId IdType="pubmed">27255185</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>mBio. 2015 Sep 08;6(5):e01280-15</Citation><ArticleIdList><ArticleId IdType="pubmed">26350969</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2018 Mar 20;115(12):3144-3149</Citation><ArticleIdList><ArticleId IdType="pubmed">29507189</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>mBio. 2013 Sep 10;4(5):e00650-13</Citation><ArticleIdList><ArticleId IdType="pubmed">24023385</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Protein Cell. 2013 Dec;4(12):951-61</Citation><ArticleIdList><ArticleId IdType="pubmed">24318862</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Emerg Infect Dis. 2016 Apr;22(4):716-9</Citation><ArticleIdList><ArticleId IdType="pubmed">26981770</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2009 Oct;83(19):10314-8</Citation><ArticleIdList><ArticleId IdType="pubmed">19640993</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Open Forum Infect Dis. 2016 Aug 03;3(3):ofw165</Citation><ArticleIdList><ArticleId IdType="pubmed">27704019</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Emerg Microbes Infect. 2017 Nov 8;6(11):e101</Citation><ArticleIdList><ArticleId IdType="pubmed">29116217</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Open Forum Infect Dis. 2018 Apr 28;5(5):ofy095</Citation><ArticleIdList><ArticleId IdType="pubmed">30294616</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000400 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000400 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:31592752
|texte= Isolation and growth characterization of novel full length and deletion mutant human MERS-CoV strains from clinical specimens collected during 2015.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:31592752" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |