Multiplex Screening Assay for Identifying Cytotoxic CD8+ T Cell Epitopes.
Identifieur interne : 000126 ( PubMed/Corpus ); précédent : 000125; suivant : 000127Multiplex Screening Assay for Identifying Cytotoxic CD8+ T Cell Epitopes.
Auteurs : Chek Meng Poh ; Jian Zheng ; Rudragouda Channappanavar ; Zi Wei Chang ; Thi H O. Nguyen ; Laurent Rénia ; Katherine Kedzierska ; Stanley Perlman ; Leo L M. PoonSource :
- Frontiers in immunology [ 1664-3224 ] ; 2020.
Abstract
The cytotoxicity of epitope-specific CD8+ T cells is usually measured indirectly through IFNγ production. Existing assays that directly measure this activity are limited mainly to measurements of up to two specificities in a single reaction. Here, we develop a multiplex cytotoxicity assay that allows direct, simultaneous measurement of up to 23 different specificities of CD8+ T cells in a single reaction. This can greatly reduce the amount of starting clinical materials for a systematic screening of CD8+ T cell epitopes. In addition, this greatly enhanced capacity enables the incorporation of irrelevant epitopes for determining the non-specific killing activity of CD8+ T cells, thereby allowing to measure the actual epitope-specific cytotoxicity activities. This technique is shown to be useful to study both human and mouse CD8+ T cells. Besides, our results from human PBMCs and three independent infectious animal models (MERS, influenza and malaria) further reveal that IFNγ expression by epitope-specific CD8+ T cells does not always correlate with their cell-killing potential, highlighting the need for using cytotoxicity assays in specific contexts (e.g., evaluating vaccine candidates). Overall, our approach opens up new possibilities for comprehensive analyses of CD8+ T cell cytotoxicity in a practical manner.
DOI: 10.3389/fimmu.2020.00400
PubMed: 32218786
Links to Exploration step
pubmed:32218786Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Multiplex Screening Assay for Identifying Cytotoxic CD8<sup>+</sup> T Cell Epitopes.</title><author><name sortKey="Poh, Chek Meng" sort="Poh, Chek Meng" uniqKey="Poh C" first="Chek Meng" last="Poh">Chek Meng Poh</name><affiliation><nlm:affiliation>School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zheng, Jian" sort="Zheng, Jian" uniqKey="Zheng J" first="Jian" last="Zheng">Jian Zheng</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, IA, United States.</nlm:affiliation></affiliation></author><author><name sortKey="Channappanavar, Rudragouda" sort="Channappanavar, Rudragouda" uniqKey="Channappanavar R" first="Rudragouda" last="Channappanavar">Rudragouda Channappanavar</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, IA, United States.</nlm:affiliation></affiliation></author><author><name sortKey="Chang, Zi Wei" sort="Chang, Zi Wei" uniqKey="Chang Z" first="Zi Wei" last="Chang">Zi Wei Chang</name><affiliation><nlm:affiliation>Singapore Immunology Network, Agency of Science, Technology and Research, Singapore, Singapore.</nlm:affiliation></affiliation></author><author><name sortKey="Nguyen, Thi H O" sort="Nguyen, Thi H O" uniqKey="Nguyen T" first="Thi H O" last="Nguyen">Thi H O. Nguyen</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Renia, Laurent" sort="Renia, Laurent" uniqKey="Renia L" first="Laurent" last="Rénia">Laurent Rénia</name><affiliation><nlm:affiliation>Singapore Immunology Network, Agency of Science, Technology and Research, Singapore, Singapore.</nlm:affiliation></affiliation></author><author><name sortKey="Kedzierska, Katherine" sort="Kedzierska, Katherine" uniqKey="Kedzierska K" first="Katherine" last="Kedzierska">Katherine Kedzierska</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, IA, United States.</nlm:affiliation></affiliation></author><author><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L M" last="Poon">Leo L M. Poon</name><affiliation><nlm:affiliation>School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32218786</idno><idno type="pmid">32218786</idno><idno type="doi">10.3389/fimmu.2020.00400</idno><idno type="wicri:Area/PubMed/Corpus">000126</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000126</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Multiplex Screening Assay for Identifying Cytotoxic CD8<sup>+</sup> T Cell Epitopes.</title><author><name sortKey="Poh, Chek Meng" sort="Poh, Chek Meng" uniqKey="Poh C" first="Chek Meng" last="Poh">Chek Meng Poh</name><affiliation><nlm:affiliation>School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zheng, Jian" sort="Zheng, Jian" uniqKey="Zheng J" first="Jian" last="Zheng">Jian Zheng</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, IA, United States.</nlm:affiliation></affiliation></author><author><name sortKey="Channappanavar, Rudragouda" sort="Channappanavar, Rudragouda" uniqKey="Channappanavar R" first="Rudragouda" last="Channappanavar">Rudragouda Channappanavar</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, IA, United States.</nlm:affiliation></affiliation></author><author><name sortKey="Chang, Zi Wei" sort="Chang, Zi Wei" uniqKey="Chang Z" first="Zi Wei" last="Chang">Zi Wei Chang</name><affiliation><nlm:affiliation>Singapore Immunology Network, Agency of Science, Technology and Research, Singapore, Singapore.</nlm:affiliation></affiliation></author><author><name sortKey="Nguyen, Thi H O" sort="Nguyen, Thi H O" uniqKey="Nguyen T" first="Thi H O" last="Nguyen">Thi H O. Nguyen</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Renia, Laurent" sort="Renia, Laurent" uniqKey="Renia L" first="Laurent" last="Rénia">Laurent Rénia</name><affiliation><nlm:affiliation>Singapore Immunology Network, Agency of Science, Technology and Research, Singapore, Singapore.</nlm:affiliation></affiliation></author><author><name sortKey="Kedzierska, Katherine" sort="Kedzierska, Katherine" uniqKey="Kedzierska K" first="Katherine" last="Kedzierska">Katherine Kedzierska</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, IA, United States.</nlm:affiliation></affiliation></author><author><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L M" last="Poon">Leo L M. Poon</name><affiliation><nlm:affiliation>School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Frontiers in immunology</title><idno type="eISSN">1664-3224</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The cytotoxicity of epitope-specific CD8<sup>+</sup> T cells is usually measured indirectly through IFNγ production. Existing assays that directly measure this activity are limited mainly to measurements of up to two specificities in a single reaction. Here, we develop a multiplex cytotoxicity assay that allows direct, simultaneous measurement of up to 23 different specificities of CD8<sup>+</sup> T cells in a single reaction. This can greatly reduce the amount of starting clinical materials for a systematic screening of CD8<sup>+</sup> T cell epitopes. In addition, this greatly enhanced capacity enables the incorporation of irrelevant epitopes for determining the non-specific killing activity of CD8<sup>+</sup> T cells, thereby allowing to measure the actual epitope-specific cytotoxicity activities. This technique is shown to be useful to study both human and mouse CD8<sup>+</sup> T cells. Besides, our results from human PBMCs and three independent infectious animal models (MERS, influenza and malaria) further reveal that IFNγ expression by epitope-specific CD8<sup>+</sup> T cells does not always correlate with their cell-killing potential, highlighting the need for using cytotoxicity assays in specific contexts (e.g., evaluating vaccine candidates). Overall, our approach opens up new possibilities for comprehensive analyses of CD8<sup>+</sup> T cell cytotoxicity in a practical manner.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">32218786</PMID><DateRevised><Year>2020</Year><Month>03</Month><Day>31</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1664-3224</ISSN><JournalIssue CitedMedium="Internet"><Volume>11</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Frontiers in immunology</Title><ISOAbbreviation>Front Immunol</ISOAbbreviation></Journal><ArticleTitle>Multiplex Screening Assay for Identifying Cytotoxic CD8<sup>+</sup> T Cell Epitopes.</ArticleTitle><Pagination><MedlinePgn>400</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fimmu.2020.00400</ELocationID><Abstract><AbstractText>The cytotoxicity of epitope-specific CD8<sup>+</sup> T cells is usually measured indirectly through IFNγ production. Existing assays that directly measure this activity are limited mainly to measurements of up to two specificities in a single reaction. Here, we develop a multiplex cytotoxicity assay that allows direct, simultaneous measurement of up to 23 different specificities of CD8<sup>+</sup> T cells in a single reaction. This can greatly reduce the amount of starting clinical materials for a systematic screening of CD8<sup>+</sup> T cell epitopes. In addition, this greatly enhanced capacity enables the incorporation of irrelevant epitopes for determining the non-specific killing activity of CD8<sup>+</sup> T cells, thereby allowing to measure the actual epitope-specific cytotoxicity activities. This technique is shown to be useful to study both human and mouse CD8<sup>+</sup> T cells. Besides, our results from human PBMCs and three independent infectious animal models (MERS, influenza and malaria) further reveal that IFNγ expression by epitope-specific CD8<sup>+</sup> T cells does not always correlate with their cell-killing potential, highlighting the need for using cytotoxicity assays in specific contexts (e.g., evaluating vaccine candidates). Overall, our approach opens up new possibilities for comprehensive analyses of CD8<sup>+</sup> T cell cytotoxicity in a practical manner.</AbstractText><CopyrightInformation>Copyright © 2020 Poh, Zheng, Channappanavar, Chang, Nguyen, Rénia, Kedzierska, Perlman and Poon.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Poh</LastName><ForeName>Chek Meng</ForeName><Initials>CM</Initials><AffiliationInfo><Affiliation>School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Jian</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, IA, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Channappanavar</LastName><ForeName>Rudragouda</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, IA, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chang</LastName><ForeName>Zi Wei</ForeName><Initials>ZW</Initials><AffiliationInfo><Affiliation>Singapore Immunology Network, Agency of Science, Technology and Research, Singapore, Singapore.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nguyen</LastName><ForeName>Thi H O</ForeName><Initials>THO</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rénia</LastName><ForeName>Laurent</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Singapore Immunology Network, Agency of Science, Technology and Research, Singapore, Singapore.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kedzierska</LastName><ForeName>Katherine</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Perlman</LastName><ForeName>Stanley</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, IA, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Poon</LastName><ForeName>Leo L M</ForeName><Initials>LLM</Initials><AffiliationInfo><Affiliation>School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>03</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Immunol</MedlineTA><NlmUniqueID>101560960</NlmUniqueID><ISSNLinking>1664-3224</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">CD8 T cell</Keyword><Keyword MajorTopicYN="N">MERS</Keyword><Keyword MajorTopicYN="N">Plasmodium</Keyword><Keyword MajorTopicYN="N">cytotoxicity</Keyword><Keyword MajorTopicYN="N">influenza</Keyword><Keyword MajorTopicYN="N">multiplex assay</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>11</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>02</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>3</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>3</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>3</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32218786</ArticleId><ArticleId IdType="doi">10.3389/fimmu.2020.00400</ArticleId><ArticleId IdType="pmc">PMC7078160</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Sci Transl Med. 2018 Feb 14;10(428):</Citation><ArticleIdList><ArticleId IdType="pubmed">29444980</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Cell Death Dis. 2017 Jun 1;8(6):e2836</Citation><ArticleIdList><ArticleId IdType="pubmed">28569770</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Immunol. 2004 Jul 1;173(1):456-62</Citation><ArticleIdList><ArticleId IdType="pubmed">15210805</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Cytometry A. 2016 Mar;89(3):246-58</Citation><ArticleIdList><ArticleId IdType="pubmed">26848928</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 1995 Jun 2;268(5215):1358-62</Citation><ArticleIdList><ArticleId IdType="pubmed">7761856</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Trends Immunol. 2017 Jun;38(6):432-443</Citation><ArticleIdList><ArticleId IdType="pubmed">28499492</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2014 Apr 1;111(13):4970-5</Citation><ArticleIdList><ArticleId IdType="pubmed">24599590</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2005 Dec;79(24):15547-55</Citation><ArticleIdList><ArticleId IdType="pubmed">16306625</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Immunity. 2016 Feb 16;44(2):233-45</Citation><ArticleIdList><ArticleId IdType="pubmed">26872694</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Immunol. 2005 Nov 1;175(9):5931-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16237086</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>BMC Genomics. 2018 Jan 19;19(Suppl 1):42</Citation><ArticleIdList><ArticleId IdType="pubmed">29363421</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Immunity. 2011 Aug 26;35(2):161-8</Citation><ArticleIdList><ArticleId IdType="pubmed">21867926</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Methods. 2009 Jul;6(7):520-6</Citation><ArticleIdList><ArticleId IdType="pubmed">19543285</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Sci Rep. 2011;1:118</Citation><ArticleIdList><ArticleId IdType="pubmed">22355635</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Immunol. 2007 Aug 15;179(4):2115-25</Citation><ArticleIdList><ArticleId IdType="pubmed">17675470</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Immunology. 1968 Feb;14(2):181-96</Citation><ArticleIdList><ArticleId IdType="pubmed">4966657</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Immunol. 2001 Nov 15;167(10):5574-82</Citation><ArticleIdList><ArticleId IdType="pubmed">11698428</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Front Immunol. 2018 Jan 22;9:14</Citation><ArticleIdList><ArticleId IdType="pubmed">29403496</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Cell Host Microbe. 2014 Jan 15;15(1):23-35</Citation><ArticleIdList><ArticleId IdType="pubmed">24439895</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Clin Invest. 2011 Nov;121(11):4322-31</Citation><ArticleIdList><ArticleId IdType="pubmed">21965332</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2000 Nov 17;290(5495):1354-8</Citation><ArticleIdList><ArticleId IdType="pubmed">11082062</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Am J Trop Med Hyg. 1965 Sep;14(5):700-14</Citation><ArticleIdList><ArticleId IdType="pubmed">5829127</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Immunol Methods. 1980;33(1):33-44</Citation><ArticleIdList><ArticleId IdType="pubmed">6444653</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Clin Invest. 2010 Jan;120(1):191-202</Citation><ArticleIdList><ArticleId IdType="pubmed">19955656</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2002 May;76(9):4251-9</Citation><ArticleIdList><ArticleId IdType="pubmed">11932390</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Leukoc Biol. 2004 Feb;75(2):163-89</Citation><ArticleIdList><ArticleId IdType="pubmed">14525967</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Diabetes. 2004 Mar;53(3):591-6</Citation><ArticleIdList><ArticleId IdType="pubmed">14988242</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Clin Vaccine Immunol. 2015 Jul;22(7):828-35</Citation><ArticleIdList><ArticleId IdType="pubmed">25994549</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Immunology. 2009 Sep;128(1 Suppl):e589-99</Citation><ArticleIdList><ArticleId IdType="pubmed">19740320</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Clin Cancer Res. 2015 Nov 15;21(22):5047-56</Citation><ArticleIdList><ArticleId IdType="pubmed">26567364</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2014 Apr 15;111(15):5676-81</Citation><ArticleIdList><ArticleId IdType="pubmed">24706798</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Infect Dis. 2004 Nov 1;190(9):1692-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15478077</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Am J Respir Cell Mol Biol. 1999 May;20(5):849-58</Citation><ArticleIdList><ArticleId IdType="pubmed">10226053</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Cell Immunol. 1996 May 1;169(2):288-93</Citation><ArticleIdList><ArticleId IdType="pubmed">8620557</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 1998 Jul;72(7):5948-54</Citation><ArticleIdList><ArticleId IdType="pubmed">9621057</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000126 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000126 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:32218786
|texte= Multiplex Screening Assay for Identifying Cytotoxic CD8+ T Cell Epitopes.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:32218786" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |