Built-in RNA-mediated Chaperone (Chaperna) for Antigen Folding Tailored to Immunized Hosts.
Identifieur interne : 000013 ( PubMed/Corpus ); précédent : 000012; suivant : 000014Built-in RNA-mediated Chaperone (Chaperna) for Antigen Folding Tailored to Immunized Hosts.
Auteurs : Young-Seok Kim ; Jongkwan Lim ; Jemin Sung ; Yucheol Cheong ; Eun-Young Lee ; Jihoon Kim ; Hana Oh ; Yeon-Sook Kim ; Nam-Hyuk Cho ; Seongil Choi ; Sang-Moo Kang ; Jae-Hwan Nam ; Wonil Chae ; Baik L. SeongSource :
- Biotechnology and bioengineering [ 1097-0290 ] ; 2020.
Abstract
High-quality antibody (Ab) production depends on the availability of immunologically relevant antigens. We present a potentially universal platform for generating soluble antigens from bacterial hosts, tailored to immunized animals for Ab production. A novel RNA-dependent chaperone, in which the target antigen is genetically fused with an RNA-interacting domain (RID) docking tag derived from the immunized host, promotes the solubility and robust folding of the target antigen. We selected the N-terminal tRNA-binding domain of lysyl-tRNA synthetase (LysRS) as the RID for fusion with viral proteins and demonstrated the expression of the RID fusion proteins in their soluble and native conformations; immunization predominantly elicited Ab responses to the target antigen, whereas the "self" RID tag remained non-immunogenic. Differential immunogenicity of the fusion proteins greatly enriched and simplified the screening of hybridoma clones of monoclonal antibodies (mAbs), enabling specific and sensitive serodiagnosis of MERS-CoV infection. Moreover, mAbs against the consensus influenza hemagglutinin stalk domain enabled a novel assay for trivalent seasonal influenza vaccines. The Fc-mediated effector function was demonstrated, which could be harnessed for the design of next-generation "universal" influenza vaccines. The non-immunogenic built-in antigen folding module tailored to a repertoire of immunized animal hosts will drive immunochemical diagnostics, therapeutics and designer vaccines. This article is protected by copyright. All rights reserved.
DOI: 10.1002/bit.27355
PubMed: 32297972
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Built-in RNA-mediated Chaperone (Chaperna) for Antigen Folding Tailored to Immunized Hosts.</title><author><name sortKey="Kim, Young Seok" sort="Kim, Young Seok" uniqKey="Kim Y" first="Young-Seok" last="Kim">Young-Seok Kim</name><affiliation><nlm:affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Lim, Jongkwan" sort="Lim, Jongkwan" uniqKey="Lim J" first="Jongkwan" last="Lim">Jongkwan Lim</name><affiliation><nlm:affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Sung, Jemin" sort="Sung, Jemin" uniqKey="Sung J" first="Jemin" last="Sung">Jemin Sung</name><affiliation><nlm:affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Cheong, Yucheol" sort="Cheong, Yucheol" uniqKey="Cheong Y" first="Yucheol" last="Cheong">Yucheol Cheong</name><affiliation><nlm:affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Lee, Eun Young" sort="Lee, Eun Young" uniqKey="Lee E" first="Eun-Young" last="Lee">Eun-Young Lee</name><affiliation><nlm:affiliation>Department of Biotechnology, The Catholic University of Korea, Bucheon, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Kim, Jihoon" sort="Kim, Jihoon" uniqKey="Kim J" first="Jihoon" last="Kim">Jihoon Kim</name><affiliation><nlm:affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Oh, Hana" sort="Oh, Hana" uniqKey="Oh H" first="Hana" last="Oh">Hana Oh</name><affiliation><nlm:affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Kim, Yeon Sook" sort="Kim, Yeon Sook" uniqKey="Kim Y" first="Yeon-Sook" last="Kim">Yeon-Sook Kim</name><affiliation><nlm:affiliation>Division of Infectious Diseases, Department of Internal Medicine, Chungnam National University School of Medicine, Daejeon, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Cho, Nam Hyuk" sort="Cho, Nam Hyuk" uniqKey="Cho N" first="Nam-Hyuk" last="Cho">Nam-Hyuk Cho</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Choi, Seongil" sort="Choi, Seongil" uniqKey="Choi S" first="Seongil" last="Choi">Seongil Choi</name><affiliation><nlm:affiliation>Department of Biochemistry and Biophysics, Stockholm University, SE-106 91, Stockholm, Sweden.</nlm:affiliation></affiliation></author><author><name sortKey="Kang, Sang Moo" sort="Kang, Sang Moo" uniqKey="Kang S" first="Sang-Moo" last="Kang">Sang-Moo Kang</name><affiliation><nlm:affiliation>Center for Inflammation, Immunity & Infection, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Nam, Jae Hwan" sort="Nam, Jae Hwan" uniqKey="Nam J" first="Jae-Hwan" last="Nam">Jae-Hwan Nam</name><affiliation><nlm:affiliation>Department of Biotechnology, The Catholic University of Korea, Bucheon, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Chae, Wonil" sort="Chae, Wonil" uniqKey="Chae W" first="Wonil" last="Chae">Wonil Chae</name><affiliation><nlm:affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Seong, Baik L" sort="Seong, Baik L" uniqKey="Seong B" first="Baik L" last="Seong">Baik L. Seong</name><affiliation><nlm:affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32297972</idno><idno type="pmid">32297972</idno><idno type="doi">10.1002/bit.27355</idno><idno type="wicri:Area/PubMed/Corpus">000013</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000013</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Built-in RNA-mediated Chaperone (Chaperna) for Antigen Folding Tailored to Immunized Hosts.</title><author><name sortKey="Kim, Young Seok" sort="Kim, Young Seok" uniqKey="Kim Y" first="Young-Seok" last="Kim">Young-Seok Kim</name><affiliation><nlm:affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Lim, Jongkwan" sort="Lim, Jongkwan" uniqKey="Lim J" first="Jongkwan" last="Lim">Jongkwan Lim</name><affiliation><nlm:affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Sung, Jemin" sort="Sung, Jemin" uniqKey="Sung J" first="Jemin" last="Sung">Jemin Sung</name><affiliation><nlm:affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Cheong, Yucheol" sort="Cheong, Yucheol" uniqKey="Cheong Y" first="Yucheol" last="Cheong">Yucheol Cheong</name><affiliation><nlm:affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Lee, Eun Young" sort="Lee, Eun Young" uniqKey="Lee E" first="Eun-Young" last="Lee">Eun-Young Lee</name><affiliation><nlm:affiliation>Department of Biotechnology, The Catholic University of Korea, Bucheon, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Kim, Jihoon" sort="Kim, Jihoon" uniqKey="Kim J" first="Jihoon" last="Kim">Jihoon Kim</name><affiliation><nlm:affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Oh, Hana" sort="Oh, Hana" uniqKey="Oh H" first="Hana" last="Oh">Hana Oh</name><affiliation><nlm:affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Kim, Yeon Sook" sort="Kim, Yeon Sook" uniqKey="Kim Y" first="Yeon-Sook" last="Kim">Yeon-Sook Kim</name><affiliation><nlm:affiliation>Division of Infectious Diseases, Department of Internal Medicine, Chungnam National University School of Medicine, Daejeon, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Cho, Nam Hyuk" sort="Cho, Nam Hyuk" uniqKey="Cho N" first="Nam-Hyuk" last="Cho">Nam-Hyuk Cho</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Choi, Seongil" sort="Choi, Seongil" uniqKey="Choi S" first="Seongil" last="Choi">Seongil Choi</name><affiliation><nlm:affiliation>Department of Biochemistry and Biophysics, Stockholm University, SE-106 91, Stockholm, Sweden.</nlm:affiliation></affiliation></author><author><name sortKey="Kang, Sang Moo" sort="Kang, Sang Moo" uniqKey="Kang S" first="Sang-Moo" last="Kang">Sang-Moo Kang</name><affiliation><nlm:affiliation>Center for Inflammation, Immunity & Infection, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Nam, Jae Hwan" sort="Nam, Jae Hwan" uniqKey="Nam J" first="Jae-Hwan" last="Nam">Jae-Hwan Nam</name><affiliation><nlm:affiliation>Department of Biotechnology, The Catholic University of Korea, Bucheon, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Chae, Wonil" sort="Chae, Wonil" uniqKey="Chae W" first="Wonil" last="Chae">Wonil Chae</name><affiliation><nlm:affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Seong, Baik L" sort="Seong, Baik L" uniqKey="Seong B" first="Baik L" last="Seong">Baik L. Seong</name><affiliation><nlm:affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Biotechnology and bioengineering</title><idno type="eISSN">1097-0290</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">High-quality antibody (Ab) production depends on the availability of immunologically relevant antigens. We present a potentially universal platform for generating soluble antigens from bacterial hosts, tailored to immunized animals for Ab production. A novel RNA-dependent chaperone, in which the target antigen is genetically fused with an RNA-interacting domain (RID) docking tag derived from the immunized host, promotes the solubility and robust folding of the target antigen. We selected the N-terminal tRNA-binding domain of lysyl-tRNA synthetase (LysRS) as the RID for fusion with viral proteins and demonstrated the expression of the RID fusion proteins in their soluble and native conformations; immunization predominantly elicited Ab responses to the target antigen, whereas the "self" RID tag remained non-immunogenic. Differential immunogenicity of the fusion proteins greatly enriched and simplified the screening of hybridoma clones of monoclonal antibodies (mAbs), enabling specific and sensitive serodiagnosis of MERS-CoV infection. Moreover, mAbs against the consensus influenza hemagglutinin stalk domain enabled a novel assay for trivalent seasonal influenza vaccines. The Fc-mediated effector function was demonstrated, which could be harnessed for the design of next-generation "universal" influenza vaccines. The non-immunogenic built-in antigen folding module tailored to a repertoire of immunized animal hosts will drive immunochemical diagnostics, therapeutics and designer vaccines. This article is protected by copyright. All rights reserved.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">32297972</PMID><DateRevised><Year>2020</Year><Month>04</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1097-0290</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Apr</Month><Day>16</Day></PubDate></JournalIssue><Title>Biotechnology and bioengineering</Title><ISOAbbreviation>Biotechnol. Bioeng.</ISOAbbreviation></Journal><ArticleTitle>Built-in RNA-mediated Chaperone (Chaperna) for Antigen Folding Tailored to Immunized Hosts.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1002/bit.27355</ELocationID><Abstract><AbstractText>High-quality antibody (Ab) production depends on the availability of immunologically relevant antigens. We present a potentially universal platform for generating soluble antigens from bacterial hosts, tailored to immunized animals for Ab production. A novel RNA-dependent chaperone, in which the target antigen is genetically fused with an RNA-interacting domain (RID) docking tag derived from the immunized host, promotes the solubility and robust folding of the target antigen. We selected the N-terminal tRNA-binding domain of lysyl-tRNA synthetase (LysRS) as the RID for fusion with viral proteins and demonstrated the expression of the RID fusion proteins in their soluble and native conformations; immunization predominantly elicited Ab responses to the target antigen, whereas the "self" RID tag remained non-immunogenic. Differential immunogenicity of the fusion proteins greatly enriched and simplified the screening of hybridoma clones of monoclonal antibodies (mAbs), enabling specific and sensitive serodiagnosis of MERS-CoV infection. Moreover, mAbs against the consensus influenza hemagglutinin stalk domain enabled a novel assay for trivalent seasonal influenza vaccines. The Fc-mediated effector function was demonstrated, which could be harnessed for the design of next-generation "universal" influenza vaccines. The non-immunogenic built-in antigen folding module tailored to a repertoire of immunized animal hosts will drive immunochemical diagnostics, therapeutics and designer vaccines. This article is protected by copyright. All rights reserved.</AbstractText><CopyrightInformation>This article is protected by copyright. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Young-Seok</ForeName><Initials>YS</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lim</LastName><ForeName>Jongkwan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sung</LastName><ForeName>Jemin</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cheong</LastName><ForeName>Yucheol</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Eun-Young</ForeName><Initials>EY</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, The Catholic University of Korea, Bucheon, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Jihoon</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Oh</LastName><ForeName>Hana</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Yeon-Sook</ForeName><Initials>YS</Initials><AffiliationInfo><Affiliation>Division of Infectious Diseases, Department of Internal Medicine, Chungnam National University School of Medicine, Daejeon, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cho</LastName><ForeName>Nam-Hyuk</ForeName><Initials>NH</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Seongil</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Biochemistry and Biophysics, Stockholm University, SE-106 91, Stockholm, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kang</LastName><ForeName>Sang-Moo</ForeName><Initials>SM</Initials><AffiliationInfo><Affiliation>Center for Inflammation, Immunity & Infection, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nam</LastName><ForeName>Jae-Hwan</ForeName><Initials>JH</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, The Catholic University of Korea, Bucheon, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chae</LastName><ForeName>Wonil</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Seong</LastName><ForeName>Baik L</ForeName><Initials>BL</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-7301-082X</Identifier><AffiliationInfo><Affiliation>Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>04</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biotechnol Bioeng</MedlineTA><NlmUniqueID>7502021</NlmUniqueID><ISSNLinking>0006-3592</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">MERS-CoV</Keyword><Keyword MajorTopicYN="N">chaperna</Keyword><Keyword MajorTopicYN="N">chaperone</Keyword><Keyword MajorTopicYN="N">influenza virus</Keyword><Keyword MajorTopicYN="N">monoclonal antibody</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>4</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>4</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>4</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32297972</ArticleId><ArticleId IdType="doi">10.1002/bit.27355</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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