Stable storage conditions of Immobiline chemicals for isoelectric focusing.
Identifieur interne : 002B22 ( PubMed/Checkpoint ); précédent : 002B21; suivant : 002B23Stable storage conditions of Immobiline chemicals for isoelectric focusing.
Auteurs : B M G Veby [Suède] ; P. Pettersson ; J. Andrasko ; L. Ineva-Flygare ; U. Johannesson ; A. Görg ; W. Postel ; A. Domscheit ; P L Mauri ; P. PiettaSource :
- Journal of biochemical and biophysical methods [ 0165-022X ]
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Abstract
Most of the problems connected with the use of the Immobiline chemicals (a set of six, non-amphoteric, acrylamido buffers having pK values in the pH 3.5-9.5 interval) can be attributed to the alkaline species (with pK values 6.2, 7.0, 8.5 and 9.3). These compounds, to varying degrees are subjected to two degradation pathways: (a) hydrolysis of the amido bond, producing free acrylic acid and a diamine, the latter unable to be incorporated into the polyacrylamide matrix; (b) spontaneous auto-polymerization, producing a number of oligomers up to n-mers, able to aggregate and precipitate large proteins. Storage of their water solutions as frozen aliquots, a method widely employed, only partially alleviates the problem. Addition of trace-amounts of inhibitors, as lately adopted by the manufacturer, could only reduce the problem of auto-polymerization, but not block the hydrolysis of the amido bond. A new solution has been found, which abolishes both phenomena: storage in n-propanol. As demonstrated by gas chromatography, HPLC analyses and two-dimensional separations of complex samples, storage in organic solvent completely abolishes both hydrolysis and auto-polymerization and allows production of highly reproducible focusing patterns.
DOI: 10.1016/0165-022x(88)90026-7
PubMed: 3411080
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Ineva-Flygare</name></author><author><name sortKey="Johannesson, U" sort="Johannesson, U" uniqKey="Johannesson U" first="U" last="Johannesson">U. Johannesson</name></author><author><name sortKey="Gorg, A" sort="Gorg, A" uniqKey="Gorg A" first="A" last="Görg">A. Görg</name></author><author><name sortKey="Postel, W" sort="Postel, W" uniqKey="Postel W" first="W" last="Postel">W. Postel</name></author><author><name sortKey="Domscheit, A" sort="Domscheit, A" uniqKey="Domscheit A" first="A" last="Domscheit">A. Domscheit</name></author><author><name sortKey="Mauri, P L" sort="Mauri, P L" uniqKey="Mauri P" first="P L" last="Mauri">P L Mauri</name></author><author><name sortKey="Pietta, P" sort="Pietta, P" uniqKey="Pietta P" first="P" last="Pietta">P. 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Pettersson</name></author><author><name sortKey="Andrasko, J" sort="Andrasko, J" uniqKey="Andrasko J" first="J" last="Andrasko">J. Andrasko</name></author><author><name sortKey="Ineva Flygare, L" sort="Ineva Flygare, L" uniqKey="Ineva Flygare L" first="L" last="Ineva-Flygare">L. Ineva-Flygare</name></author><author><name sortKey="Johannesson, U" sort="Johannesson, U" uniqKey="Johannesson U" first="U" last="Johannesson">U. Johannesson</name></author><author><name sortKey="Gorg, A" sort="Gorg, A" uniqKey="Gorg A" first="A" last="Görg">A. Görg</name></author><author><name sortKey="Postel, W" sort="Postel, W" uniqKey="Postel W" first="W" last="Postel">W. Postel</name></author><author><name sortKey="Domscheit, A" sort="Domscheit, A" uniqKey="Domscheit A" first="A" last="Domscheit">A. Domscheit</name></author><author><name sortKey="Mauri, P L" sort="Mauri, P L" uniqKey="Mauri P" first="P L" last="Mauri">P L Mauri</name></author><author><name sortKey="Pietta, P" sort="Pietta, P" uniqKey="Pietta P" first="P" last="Pietta">P. Pietta</name></author></analytic><series><title level="j">Journal of biochemical and biophysical methods</title><idno type="ISSN">0165-022X</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>1-Propanol</term><term>Buffers</term><term>Chromatography, Gas</term><term>Chromatography, Gel</term><term>Chromatography, High Pressure Liquid</term><term>Drug Stability</term><term>Drug Storage</term><term>Hydrolysis</term><term>Isoelectric Focusing</term><term>Polymers</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Chromatographie en phase gazeuse</term><term>Chromatographie en phase liquide à haute performance</term><term>Chromatographie sur gel</term><term>Focalisation isoélectrique</term><term>Hydrolyse</term><term>Polymères</term><term>Propan-1-ol</term><term>Stabilité de médicament</term><term>Stockage de médicament</term><term>Substances tampon</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>1-Propanol</term><term>Buffers</term><term>Polymers</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromatography, Gas</term><term>Chromatography, Gel</term><term>Chromatography, High Pressure Liquid</term><term>Drug Stability</term><term>Drug Storage</term><term>Hydrolysis</term><term>Isoelectric Focusing</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Chromatographie en phase gazeuse</term><term>Chromatographie en phase liquide à haute performance</term><term>Chromatographie sur gel</term><term>Focalisation isoélectrique</term><term>Hydrolyse</term><term>Polymères</term><term>Propan-1-ol</term><term>Stabilité de médicament</term><term>Stockage de médicament</term><term>Substances tampon</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Most of the problems connected with the use of the Immobiline chemicals (a set of six, non-amphoteric, acrylamido buffers having pK values in the pH 3.5-9.5 interval) can be attributed to the alkaline species (with pK values 6.2, 7.0, 8.5 and 9.3). These compounds, to varying degrees are subjected to two degradation pathways: (a) hydrolysis of the amido bond, producing free acrylic acid and a diamine, the latter unable to be incorporated into the polyacrylamide matrix; (b) spontaneous auto-polymerization, producing a number of oligomers up to n-mers, able to aggregate and precipitate large proteins. Storage of their water solutions as frozen aliquots, a method widely employed, only partially alleviates the problem. Addition of trace-amounts of inhibitors, as lately adopted by the manufacturer, could only reduce the problem of auto-polymerization, but not block the hydrolysis of the amido bond. A new solution has been found, which abolishes both phenomena: storage in n-propanol. As demonstrated by gas chromatography, HPLC analyses and two-dimensional separations of complex samples, storage in organic solvent completely abolishes both hydrolysis and auto-polymerization and allows production of highly reproducible focusing patterns.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">3411080</PMID><DateCompleted><Year>1988</Year><Month>09</Month><Day>30</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>12</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0165-022X</ISSN><JournalIssue CitedMedium="Print"><Volume>16</Volume><Issue>2-3</Issue><PubDate><MedlineDate>1988 Jun-Jul</MedlineDate></PubDate></JournalIssue><Title>Journal of biochemical and biophysical methods</Title><ISOAbbreviation>J. Biochem. Biophys. Methods</ISOAbbreviation></Journal><ArticleTitle>Stable storage conditions of Immobiline chemicals for isoelectric focusing.</ArticleTitle><Pagination><MedlinePgn>141-64</MedlinePgn></Pagination><Abstract><AbstractText>Most of the problems connected with the use of the Immobiline chemicals (a set of six, non-amphoteric, acrylamido buffers having pK values in the pH 3.5-9.5 interval) can be attributed to the alkaline species (with pK values 6.2, 7.0, 8.5 and 9.3). These compounds, to varying degrees are subjected to two degradation pathways: (a) hydrolysis of the amido bond, producing free acrylic acid and a diamine, the latter unable to be incorporated into the polyacrylamide matrix; (b) spontaneous auto-polymerization, producing a number of oligomers up to n-mers, able to aggregate and precipitate large proteins. Storage of their water solutions as frozen aliquots, a method widely employed, only partially alleviates the problem. Addition of trace-amounts of inhibitors, as lately adopted by the manufacturer, could only reduce the problem of auto-polymerization, but not block the hydrolysis of the amido bond. A new solution has been found, which abolishes both phenomena: storage in n-propanol. As demonstrated by gas chromatography, HPLC analyses and two-dimensional separations of complex samples, storage in organic solvent completely abolishes both hydrolysis and auto-polymerization and allows production of highly reproducible focusing patterns.</AbstractText></Abstract><AuthorList CompleteYN="N"><Author ValidYN="Y"><LastName>Gåveby</LastName><ForeName>B M</ForeName><Initials>BM</Initials><AffiliationInfo><Affiliation>Pharmacia LKB Biotechnology AB, Bromma, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pettersson</LastName><ForeName>P</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Andrasko</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Ineva-Flygare</LastName><ForeName>L</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Johannesson</LastName><ForeName>U</ForeName><Initials>U</Initials></Author><Author ValidYN="Y"><LastName>Görg</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Postel</LastName><ForeName>W</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Domscheit</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Mauri</LastName><ForeName>P L</ForeName><Initials>PL</Initials></Author><Author ValidYN="Y"><LastName>Pietta</LastName><ForeName>P</ForeName><Initials>P</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Biochem Biophys Methods</MedlineTA><NlmUniqueID>7907378</NlmUniqueID><ISSNLinking>0165-022X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002021">Buffers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011108">Polymers</NameOfSubstance></Chemical><Chemical><RegistryNumber>96F264O9SV</RegistryNumber><NameOfSubstance UI="D000433">1-Propanol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000433" MajorTopicYN="N">1-Propanol</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002021" MajorTopicYN="N">Buffers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002849" MajorTopicYN="N">Chromatography, Gas</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002850" MajorTopicYN="N">Chromatography, Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002851" MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004355" MajorTopicYN="N">Drug Stability</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004356" MajorTopicYN="N">Drug Storage</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007525" MajorTopicYN="Y">Isoelectric Focusing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011108" MajorTopicYN="N">Polymers</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1988</Year><Month>6</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1988</Year><Month>6</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1988</Year><Month>6</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">3411080</ArticleId><ArticleId IdType="pii">0165-022X(88)90026-7</ArticleId><ArticleId IdType="doi">10.1016/0165-022x(88)90026-7</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Suède</li></country></list><tree><noCountry><name sortKey="Andrasko, J" sort="Andrasko, J" uniqKey="Andrasko J" first="J" last="Andrasko">J. Andrasko</name><name sortKey="Domscheit, A" sort="Domscheit, A" uniqKey="Domscheit A" first="A" last="Domscheit">A. Domscheit</name><name sortKey="Gorg, A" sort="Gorg, A" uniqKey="Gorg A" first="A" last="Görg">A. Görg</name><name sortKey="Ineva Flygare, L" sort="Ineva Flygare, L" uniqKey="Ineva Flygare L" first="L" last="Ineva-Flygare">L. Ineva-Flygare</name><name sortKey="Johannesson, U" sort="Johannesson, U" uniqKey="Johannesson U" first="U" last="Johannesson">U. Johannesson</name><name sortKey="Mauri, P L" sort="Mauri, P L" uniqKey="Mauri P" first="P L" last="Mauri">P L Mauri</name><name sortKey="Pettersson, P" sort="Pettersson, P" uniqKey="Pettersson P" first="P" last="Pettersson">P. Pettersson</name><name sortKey="Pietta, P" sort="Pietta, P" uniqKey="Pietta P" first="P" last="Pietta">P. Pietta</name><name sortKey="Postel, W" sort="Postel, W" uniqKey="Postel W" first="W" last="Postel">W. Postel</name></noCountry><country name="Suède"><noRegion><name sortKey="G Veby, B M" sort="G Veby, B M" uniqKey="G Veby B" first="B M" last="G Veby">B M G Veby</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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