Rapid generation of a human monoclonal antibody to combat Middle East respiratory syndrome.
Identifieur interne : 002A32 ( PubMed/Checkpoint ); précédent : 002A31; suivant : 002A33Rapid generation of a human monoclonal antibody to combat Middle East respiratory syndrome.
Auteurs : Davide Corti [Suisse] ; Nadia Passini [Suisse] ; Antonio Lanzavecchia [Suisse] ; Maria Zambon [Royaume-Uni]Source :
- Journal of infection and public health [ 1876-035X ]
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (isolement et purification), Anticorps antiviraux (pharmacologie), Anticorps monoclonaux (isolement et purification), Anticorps monoclonaux (pharmacologie), Biotechnologie (), Cellules CHO, Cricetulus, Facteurs immunologiques (isolement et purification), Facteurs immunologiques (pharmacologie), Facteurs temps, Humains, Immunothérapie (), Infections à coronavirus (), Technologie pharmaceutique ().
- MESH :
- isolement et purification : Anticorps antiviraux, Anticorps monoclonaux, Facteurs immunologiques.
- pharmacologie : Anticorps antiviraux, Anticorps monoclonaux, Facteurs immunologiques.
- Animaux, Biotechnologie, Cellules CHO, Cricetulus, Facteurs temps, Humains, Immunothérapie, Infections à coronavirus, Technologie pharmaceutique.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (isolation & purification), Antibodies, Monoclonal (pharmacology), Antibodies, Viral (isolation & purification), Antibodies, Viral (pharmacology), Biotechnology (methods), CHO Cells, Coronavirus Infections (prevention & control), Coronavirus Infections (therapy), Cricetulus, Humans, Immunologic Factors (isolation & purification), Immunologic Factors (pharmacology), Immunotherapy (methods), Technology, Pharmaceutical (methods), Time Factors.
- MESH :
- chemical , isolation & purification : Antibodies, Monoclonal, Antibodies, Viral, Immunologic Factors.
- chemical , pharmacology : Antibodies, Monoclonal, Antibodies, Viral, Immunologic Factors.
- methods : Biotechnology, Immunotherapy, Technology, Pharmaceutical.
- prevention & control : Coronavirus Infections.
- therapy : Coronavirus Infections.
- Animals, CHO Cells, Cricetulus, Humans, Time Factors.
Abstract
The last century has witnessed the emergence of several previously unknown viruses as life-threatening human pathogens. Several examples include HIV, Ebola, Lujo, and, most recently, the Middle East respiratory syndrome (MERS) and Ebola. In this study, we describe a method for the swift generation of a human-derived monoclonal antibody, known as LCA60, as a treatment for MERS infections. LCA60 antibody was generated using the Cellclone Technology from the immortalized B cells of a human donor recovering from MERS. Only four months were required from the initial screening of B cells to the development of a stable CHO cell line suitable for the production of clinical grade antibody, thereby delineating a rapid pathway for the development of antiviral therapies against emerging viruses. Currently, the LCA60 antibody is being considered for clinical development, which includes prophylaxis in individuals at risk and a treatment for severe MERS-CoV infections.
DOI: 10.1016/j.jiph.2016.04.003
PubMed: 27102927
Affiliations:
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pubmed:27102927Le document en format XML
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<front><div type="abstract" xml:lang="en">The last century has witnessed the emergence of several previously unknown viruses as life-threatening human pathogens. Several examples include HIV, Ebola, Lujo, and, most recently, the Middle East respiratory syndrome (MERS) and Ebola. In this study, we describe a method for the swift generation of a human-derived monoclonal antibody, known as LCA60, as a treatment for MERS infections. LCA60 antibody was generated using the Cellclone Technology from the immortalized B cells of a human donor recovering from MERS. Only four months were required from the initial screening of B cells to the development of a stable CHO cell line suitable for the production of clinical grade antibody, thereby delineating a rapid pathway for the development of antiviral therapies against emerging viruses. Currently, the LCA60 antibody is being considered for clinical development, which includes prophylaxis in individuals at risk and a treatment for severe MERS-CoV infections. </div>
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