Duplex stabilities of phosphorothioate, methylphosphonate, and RNA analogs of two DNA 14-mers.
Identifieur interne : 002845 ( PubMed/Checkpoint ); précédent : 002844; suivant : 002846Duplex stabilities of phosphorothioate, methylphosphonate, and RNA analogs of two DNA 14-mers.
Auteurs : L. Kibler-Herzog ; G. Zon ; B. Uznanski ; G. Whittier ; W D WilsonSource :
- Nucleic acids research [ 0305-1048 ] ; 1991.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : DNA, Organophosphorus Compounds, RNA, Thionucleotides.
- Base Sequence, Molecular Sequence Data, Nucleic Acid Heteroduplexes, Spectrophotometry, Ultraviolet, Temperature.
Abstract
The duplex stabilities of various phosphorothioate, methylphosphonate, RNA and 2'-OCH3 RNA analogs of two self-complementary DNA 14-mers are compared. Phosphorothioate and/or methylphosphonate analogs of the two sequences d(TAATTAATTAATTA) [D1] and d(TAGCTAATTAGCTA) [D2] differ in the number, position, or chirality (at the 5' terminal linkage) of the modified phosphates. Phosphorothioate derivatives of D1 are found to be less destabilized when the linkage modified is between adenines rather than between thymines. Surprisingly, no base sequence effect on duplex stabilization is observed for any methylphosphonate derivatives of D1 or D2. Highly modified phosphorothioates or methylphosphonates are less stable than their partially modified counterparts which are less stable than the unmodified parent compounds. The 'normal' (2'-OH) RNA analog of duplex D1 is slightly destabilized, whereas the 2'-OCH3 RNA derivative is significantly stabilized relative to the unmodified DNA. For the D1 sequence, at approximately physiological salt concentration, the order of duplex stability is 2'-OCH3 RNA greater than unmodified DNA greater than 'normal' RNA greater than methylphosphonate DNA greater than phosphorothioate DNA. D2 and the various D2 methylphosphonate analogs investigated all formed hairpin conformations at low salt concentrations.
DOI: 10.1093/nar/19.11.2979
PubMed: 1711677
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Kibler-Herzog</name><affiliation><nlm:affiliation>Department of Chemistry, Georgia State University, Atlanta.</nlm:affiliation><wicri:noCountry code="subField">Atlanta</wicri:noCountry></affiliation></author><author><name sortKey="Zon, G" sort="Zon, G" uniqKey="Zon G" first="G" last="Zon">G. Zon</name></author><author><name sortKey="Uznanski, B" sort="Uznanski, B" uniqKey="Uznanski B" first="B" last="Uznanski">B. Uznanski</name></author><author><name sortKey="Whittier, G" sort="Whittier, G" uniqKey="Whittier G" first="G" last="Whittier">G. Whittier</name></author><author><name sortKey="Wilson, W D" sort="Wilson, W D" uniqKey="Wilson W" first="W D" last="Wilson">W D Wilson</name></author></analytic><series><title level="j">Nucleic acids research</title><idno type="ISSN">0305-1048</idno><imprint><date when="1991" type="published">1991</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>DNA (chemistry)</term><term>Molecular Sequence Data</term><term>Nucleic Acid Heteroduplexes</term><term>Organophosphorus Compounds (chemistry)</term><term>RNA (chemistry)</term><term>Spectrophotometry, Ultraviolet</term><term>Temperature</term><term>Thionucleotides (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN ()</term><term>ARN ()</term><term>Composés organiques du phosphore ()</term><term>Données de séquences moléculaires</term><term>Hétéroduplexes d'acides nucléiques</term><term>Spectrophotométrie UV</term><term>Séquence nucléotidique</term><term>Température</term><term>Thionucléotides ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA</term><term>Organophosphorus Compounds</term><term>RNA</term><term>Thionucleotides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Molecular Sequence Data</term><term>Nucleic Acid Heteroduplexes</term><term>Spectrophotometry, Ultraviolet</term><term>Temperature</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN</term><term>ARN</term><term>Composés organiques du phosphore</term><term>Données de séquences moléculaires</term><term>Hétéroduplexes d'acides nucléiques</term><term>Spectrophotométrie UV</term><term>Séquence nucléotidique</term><term>Température</term><term>Thionucléotides</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The duplex stabilities of various phosphorothioate, methylphosphonate, RNA and 2'-OCH3 RNA analogs of two self-complementary DNA 14-mers are compared. Phosphorothioate and/or methylphosphonate analogs of the two sequences d(TAATTAATTAATTA) [D1] and d(TAGCTAATTAGCTA) [D2] differ in the number, position, or chirality (at the 5' terminal linkage) of the modified phosphates. Phosphorothioate derivatives of D1 are found to be less destabilized when the linkage modified is between adenines rather than between thymines. Surprisingly, no base sequence effect on duplex stabilization is observed for any methylphosphonate derivatives of D1 or D2. Highly modified phosphorothioates or methylphosphonates are less stable than their partially modified counterparts which are less stable than the unmodified parent compounds. The 'normal' (2'-OH) RNA analog of duplex D1 is slightly destabilized, whereas the 2'-OCH3 RNA derivative is significantly stabilized relative to the unmodified DNA. For the D1 sequence, at approximately physiological salt concentration, the order of duplex stability is 2'-OCH3 RNA greater than unmodified DNA greater than 'normal' RNA greater than methylphosphonate DNA greater than phosphorothioate DNA. D2 and the various D2 methylphosphonate analogs investigated all formed hairpin conformations at low salt concentrations.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">1711677</PMID><DateCompleted><Year>1991</Year><Month>07</Month><Day>31</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>01</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0305-1048</ISSN><JournalIssue CitedMedium="Print"><Volume>19</Volume><Issue>11</Issue><PubDate><Year>1991</Year><Month>Jun</Month><Day>11</Day></PubDate></JournalIssue><Title>Nucleic acids research</Title><ISOAbbreviation>Nucleic Acids Res.</ISOAbbreviation></Journal><ArticleTitle>Duplex stabilities of phosphorothioate, methylphosphonate, and RNA analogs of two DNA 14-mers.</ArticleTitle><Pagination><MedlinePgn>2979-86</MedlinePgn></Pagination><Abstract><AbstractText>The duplex stabilities of various phosphorothioate, methylphosphonate, RNA and 2'-OCH3 RNA analogs of two self-complementary DNA 14-mers are compared. Phosphorothioate and/or methylphosphonate analogs of the two sequences d(TAATTAATTAATTA) [D1] and d(TAGCTAATTAGCTA) [D2] differ in the number, position, or chirality (at the 5' terminal linkage) of the modified phosphates. Phosphorothioate derivatives of D1 are found to be less destabilized when the linkage modified is between adenines rather than between thymines. Surprisingly, no base sequence effect on duplex stabilization is observed for any methylphosphonate derivatives of D1 or D2. Highly modified phosphorothioates or methylphosphonates are less stable than their partially modified counterparts which are less stable than the unmodified parent compounds. The 'normal' (2'-OH) RNA analog of duplex D1 is slightly destabilized, whereas the 2'-OCH3 RNA derivative is significantly stabilized relative to the unmodified DNA. For the D1 sequence, at approximately physiological salt concentration, the order of duplex stability is 2'-OCH3 RNA greater than unmodified DNA greater than 'normal' RNA greater than methylphosphonate DNA greater than phosphorothioate DNA. D2 and the various D2 methylphosphonate analogs investigated all formed hairpin conformations at low salt concentrations.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kibler-Herzog</LastName><ForeName>L</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Chemistry, Georgia State University, Atlanta.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zon</LastName><ForeName>G</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Uznanski</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Whittier</LastName><ForeName>G</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Wilson</LastName><ForeName>W D</ForeName><Initials>WD</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>AI-27196</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United 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PubStatus="entrez"><Year>1991</Year><Month>6</Month><Day>11</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">1711677</ArticleId><ArticleId IdType="pmc">PMC328260</ArticleId><ArticleId IdType="doi">10.1093/nar/19.11.2979</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Chromatogr. 1985 Jun 19;326:263-80</Citation><ArticleIdList><ArticleId IdType="pubmed">4030945</ArticleId></ArticleIdList></Reference><Reference><Citation>Biopolymers. 1968 Apr;6(4):491-512</Citation><ArticleIdList><ArticleId IdType="pubmed">5644782</ArticleId></ArticleIdList></Reference><Reference><Citation>Oncogene Res. 1989;4(4):243-52</Citation><ArticleIdList><ArticleId IdType="pubmed">2671865</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 1989 Feb 7;28(3):1040-7</Citation><ArticleIdList><ArticleId IdType="pubmed">2713356</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1989 Feb 25;17(4):1549-61</Citation><ArticleIdList><ArticleId IdType="pubmed">2922286</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Biochem. 1988 Aug 1;172(2):289-95</Citation><ArticleIdList><ArticleId IdType="pubmed">3056098</ArticleId></ArticleIdList></Reference><Reference><Citation>Pharm Res. 1988 Sep;5(9):539-49</Citation><ArticleIdList><ArticleId IdType="pubmed">3073387</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Enzymol. 1989;180:304-25</Citation><ArticleIdList><ArticleId IdType="pubmed">2482421</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1989 Sep 25;17(18):7253-62</Citation><ArticleIdList><ArticleId IdType="pubmed">2477796</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1989 Jun;86(11):4244-8</Citation><ArticleIdList><ArticleId IdType="pubmed">2471199</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Biophys Biophys Chem. 1988;17:167-92</Citation><ArticleIdList><ArticleId IdType="pubmed">2456074</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 1987 May 11;215(2):327-30</Citation><ArticleIdList><ArticleId IdType="pubmed">2438160</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1987 Jun 11;15(11):4403-15</Citation><ArticleIdList><ArticleId IdType="pubmed">2438655</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1989 Sep;86(18):6868-72</Citation><ArticleIdList><ArticleId IdType="pubmed">2550925</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 1990 Sep 18;29(37):8747-53</Citation><ArticleIdList><ArticleId IdType="pubmed">2176833</ArticleId></ArticleIdList></Reference><Reference><Citation>Cancer Res. 1990 Oct 15;50(20):6565-70</Citation><ArticleIdList><ArticleId IdType="pubmed">2208117</ArticleId></ArticleIdList></Reference><Reference><Citation>New Biol. 1990 Dec;2(12):1091-100</Citation><ArticleIdList><ArticleId IdType="pubmed">1708281</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Biochem. 1990 Jul;188(1):11-6</Citation><ArticleIdList><ArticleId IdType="pubmed">1699444</ArticleId></ArticleIdList></Reference><Reference><Citation>Drug Metab Dispos. 1990 Sep-Oct;18(5):815-8</Citation><ArticleIdList><ArticleId IdType="pubmed">1981742</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1988 Oct;85(19):7079-83</Citation><ArticleIdList><ArticleId IdType="pubmed">3174622</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1989 Nov 25;17(22):9193-204</Citation><ArticleIdList><ArticleId IdType="pubmed">2555787</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1989 Jun 12;17(11):4255-73</Citation><ArticleIdList><ArticleId IdType="pubmed">2472605</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1986 Nov 25;14(22):9081-93</Citation><ArticleIdList><ArticleId IdType="pubmed">3786144</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1987 Jun 25;15(12):4915-30</Citation><ArticleIdList><ArticleId IdType="pubmed">3601658</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1985 Aug 12;13(15):5707-16</Citation><ArticleIdList><ArticleId IdType="pubmed">4034395</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 1985 Jul 2;24(14):3630-8</Citation><ArticleIdList><ArticleId IdType="pubmed">4041432</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1987 Nov;84(22):7896-900</Citation><ArticleIdList><ArticleId IdType="pubmed">2825169</ArticleId></ArticleIdList></Reference><Reference><Citation>Ann N Y Acad Sci. 1987;507:220-41</Citation><ArticleIdList><ArticleId IdType="pubmed">3327415</ArticleId></ArticleIdList></Reference><Reference><Citation>Anticancer Drug Des. 1989 Oct;4(3):221-32</Citation><ArticleIdList><ArticleId IdType="pubmed">2679622</ArticleId></ArticleIdList></Reference><Reference><Citation>Anticancer Drug Des. 1988 Aug;3(2):117-27</Citation><ArticleIdList><ArticleId IdType="pubmed">2457379</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Biochem. 1985;54:367-402</Citation><ArticleIdList><ArticleId IdType="pubmed">2411211</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1987 Jul 24;15(14):5749-63</Citation><ArticleIdList><ArticleId IdType="pubmed">3475677</ArticleId></ArticleIdList></Reference><Reference><Citation>Gene. 1988 Dec 10;72(1-2):267-75</Citation><ArticleIdList><ArticleId IdType="pubmed">2468567</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1989 Jan 11;17(1):239-52</Citation><ArticleIdList><ArticleId IdType="pubmed">2911465</ArticleId></ArticleIdList></Reference><Reference><Citation>Cancer Res. 1988 May 15;48(10):2659-68</Citation><ArticleIdList><ArticleId IdType="pubmed">3282648</ArticleId></ArticleIdList></Reference><Reference><Citation>Gene. 1988 Dec 10;72(1-2):343-7</Citation><ArticleIdList><ArticleId IdType="pubmed">3243433</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 1988 Aug 23;27(17):6321-6</Citation><ArticleIdList><ArticleId IdType="pubmed">3219337</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1987 Nov;84(21):7706-10</Citation><ArticleIdList><ArticleId IdType="pubmed">3499613</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1986 Jun;83(11):3746-50</Citation><ArticleIdList><ArticleId IdType="pubmed">3459152</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1987 Aug 11;15(15):6131-48</Citation><ArticleIdList><ArticleId IdType="pubmed">3627981</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 1986 Sep 9;25(18):5092-7</Citation><ArticleIdList><ArticleId IdType="pubmed">3768335</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1990 Apr 25;18(8):2109-15</Citation><ArticleIdList><ArticleId IdType="pubmed">2336391</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1990 May;87(9):3430-4</Citation><ArticleIdList><ArticleId IdType="pubmed">2333292</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1990 Apr 11;18(7):1763-9</Citation><ArticleIdList><ArticleId IdType="pubmed">1692405</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1990 Feb;87(4):1401-5</Citation><ArticleIdList><ArticleId IdType="pubmed">2154746</ArticleId></ArticleIdList></Reference><Reference><Citation>Lymphokine Res. 1990 Spring;9(1):35-42</Citation><ArticleIdList><ArticleId IdType="pubmed">2182947</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1990 Jun 25;18(12):3545-55</Citation><ArticleIdList><ArticleId IdType="pubmed">2362807</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1990 Jun 25;18(12):3537-43</Citation><ArticleIdList><ArticleId IdType="pubmed">2362806</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 1990 May 8;29(18):4446-56</Citation><ArticleIdList><ArticleId IdType="pubmed">2350548</ArticleId></ArticleIdList></Reference><Reference><Citation>Biopolymers. 1990 Jan;29(1):13-27</Citation><ArticleIdList><ArticleId IdType="pubmed">2328281</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1984 Apr 11;12(7):3387-404</Citation><ArticleIdList><ArticleId IdType="pubmed">6718253</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 1982 May 11;21(10):2507-12</Citation><ArticleIdList><ArticleId IdType="pubmed">7046789</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1980 May 24;8(10):2295-9</Citation><ArticleIdList><ArticleId IdType="pubmed">6159577</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 1979 Nov 13;18(23):5134-43</Citation><ArticleIdList><ArticleId IdType="pubmed">497173</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 1971 Jun 22;10(13):2581-7</Citation><ArticleIdList><ArticleId IdType="pubmed">4934115</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 1972 Sep 26;48(6):1392-9</Citation><ArticleIdList><ArticleId IdType="pubmed">5077825</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1970 Oct;42(2):421-8</Citation><ArticleIdList><ArticleId IdType="pubmed">4321302</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 1974 Jul 15;86(4):843-53</Citation><ArticleIdList><ArticleId IdType="pubmed">4427357</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 1966 Sep;20(2):359-89</Citation><ArticleIdList><ArticleId IdType="pubmed">5339332</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 1968 Sep 28;36(3):291-304</Citation><ArticleIdList><ArticleId IdType="pubmed">5760542</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1989 Oct;86(20):7790-4</Citation><ArticleIdList><ArticleId IdType="pubmed">2682627</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Kibler Herzog, L" sort="Kibler Herzog, L" uniqKey="Kibler Herzog L" first="L" last="Kibler-Herzog">L. Kibler-Herzog</name><name sortKey="Uznanski, B" sort="Uznanski, B" uniqKey="Uznanski B" first="B" last="Uznanski">B. Uznanski</name><name sortKey="Whittier, G" sort="Whittier, G" uniqKey="Whittier G" first="G" last="Whittier">G. Whittier</name><name sortKey="Wilson, W D" sort="Wilson, W D" uniqKey="Wilson W" first="W D" last="Wilson">W D Wilson</name><name sortKey="Zon, G" sort="Zon, G" uniqKey="Zon G" first="G" last="Zon">G. Zon</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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