IciA protein, a specific inhibitor of initiation of Escherichia coli chromosomal replication.
Identifieur interne : 002808 ( PubMed/Checkpoint ); précédent : 002807; suivant : 002809IciA protein, a specific inhibitor of initiation of Escherichia coli chromosomal replication.
Auteurs : D S Hwang [États-Unis] ; B. Thöny ; A. KornbergSource :
- The Journal of biological chemistry [ 0021-9258 ] ; 1992.
Descripteurs français
- KwdFr :
- ADN bactérien (biosynthèse), Chromosomes de bactérie, Données de séquences moléculaires, Escherichia coli (génétique), Plasmides, Protéines Escherichia coli, Protéines bactériennes (biosynthèse), Protéines bactériennes (génétique), Protéines bactériennes (métabolisme), Protéines de liaison à l'ADN (biosynthèse), Protéines de liaison à l'ADN (génétique), Protéines de liaison à l'ADN (métabolisme), Réplication de l'ADN, Séquence nucléotidique, Électrophorèse sur gel de polyacrylamide.
- MESH :
- biosynthèse : ADN bactérien, Protéines bactériennes, Protéines de liaison à l'ADN.
- génétique : Escherichia coli, Protéines bactériennes, Protéines de liaison à l'ADN.
- métabolisme : Protéines bactériennes, Protéines de liaison à l'ADN.
- Chromosomes de bactérie, Données de séquences moléculaires, Plasmides, Protéines Escherichia coli, Réplication de l'ADN, Séquence nucléotidique, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Bacterial Proteins (biosynthesis), Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Base Sequence, Chromosomes, Bacterial, DNA Replication, DNA, Bacterial (biosynthesis), DNA-Binding Proteins (biosynthesis), DNA-Binding Proteins (genetics), DNA-Binding Proteins (metabolism), Electrophoresis, Polyacrylamide Gel, Escherichia coli (genetics), Escherichia coli Proteins, Molecular Sequence Data, Plasmids.
- MESH :
- chemical , biosynthesis : Bacterial Proteins, DNA, Bacterial, DNA-Binding Proteins.
- chemical , genetics : Bacterial Proteins, DNA-Binding Proteins.
- chemical , metabolism : Bacterial Proteins, DNA-Binding Proteins.
- genetics : Escherichia coli.
- Base Sequence, Chromosomes, Bacterial, DNA Replication, Electrophoresis, Polyacrylamide Gel, Escherichia coli Proteins, Molecular Sequence Data, Plasmids.
Abstract
Specific binding of IciA protein to the 13-mers in the origin of a minichromosome (oriC) inhibits initiation of replication in vitro by blocking the opening of this region effected by the initiator DnaA protein (Hwang, D.S., and Kornberg, A. (1990) Cell 63, 325-331). Isolation of the iciA gene (Thöny, B., Hwang, D.S., Fradkin, L., and Kornberg, A. (1991) Proc. Natl. Acad. Sci. U.S.A. 88, 4066-4070) has made possible the construction of an IciA-overproducing strain, which in turn has simplified the isolation of a large quantity of the protein, indistinguishable from that of the wild-type strain. Based on sedimentation and gel filtration, the IciA protein is an elongated dimer of a 33.4-kDa subunit. The specific binding of IciA protein to the 13-mers was stable for 2 h at 30 degrees C. The amounts of IciA protein, detected by immunoassays, increased 4-fold compared with levels (about 100 dimers) in log-phase cells whereas levels of DnaA protein decreased upon entry of cells into the stationary phase.
PubMed: 1733927
Affiliations:
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pubmed:1733927Le document en format XML
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<term>Chromosomes, Bacterial</term>
<term>DNA Replication</term>
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<term>Plasmides</term>
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<term>Réplication de l'ADN</term>
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<front><div type="abstract" xml:lang="en">Specific binding of IciA protein to the 13-mers in the origin of a minichromosome (oriC) inhibits initiation of replication in vitro by blocking the opening of this region effected by the initiator DnaA protein (Hwang, D.S., and Kornberg, A. (1990) Cell 63, 325-331). Isolation of the iciA gene (Thöny, B., Hwang, D.S., Fradkin, L., and Kornberg, A. (1991) Proc. Natl. Acad. Sci. U.S.A. 88, 4066-4070) has made possible the construction of an IciA-overproducing strain, which in turn has simplified the isolation of a large quantity of the protein, indistinguishable from that of the wild-type strain. Based on sedimentation and gel filtration, the IciA protein is an elongated dimer of a 33.4-kDa subunit. The specific binding of IciA protein to the 13-mers was stable for 2 h at 30 degrees C. The amounts of IciA protein, detected by immunoassays, increased 4-fold compared with levels (about 100 dimers) in log-phase cells whereas levels of DnaA protein decreased upon entry of cells into the stationary phase.</div>
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<Title>The Journal of biological chemistry</Title>
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<ArticleTitle>IciA protein, a specific inhibitor of initiation of Escherichia coli chromosomal replication.</ArticleTitle>
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<Abstract><AbstractText>Specific binding of IciA protein to the 13-mers in the origin of a minichromosome (oriC) inhibits initiation of replication in vitro by blocking the opening of this region effected by the initiator DnaA protein (Hwang, D.S., and Kornberg, A. (1990) Cell 63, 325-331). Isolation of the iciA gene (Thöny, B., Hwang, D.S., Fradkin, L., and Kornberg, A. (1991) Proc. Natl. Acad. Sci. U.S.A. 88, 4066-4070) has made possible the construction of an IciA-overproducing strain, which in turn has simplified the isolation of a large quantity of the protein, indistinguishable from that of the wild-type strain. Based on sedimentation and gel filtration, the IciA protein is an elongated dimer of a 33.4-kDa subunit. The specific binding of IciA protein to the 13-mers was stable for 2 h at 30 degrees C. The amounts of IciA protein, detected by immunoassays, increased 4-fold compared with levels (about 100 dimers) in log-phase cells whereas levels of DnaA protein decreased upon entry of cells into the stationary phase.</AbstractText>
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