MersV1, PubMed, Checkpoint, bibRecord, 002709

Synthetic polyamines stimulate in vitro transcription by T7 RNA polymerase.

Identifieur interne : 002709 ( PubMed/Checkpoint ); précédent : 002708; suivant : 002710

Synthetic polyamines stimulate in vitro transcription by T7 RNA polymerase.

Auteurs : M. Frugier [France] ; C. Florentz ; M W Hosseini ; J M Lehn ; R. Giegé

Source :

Nucleic acids research [ 0305-1048 ] ; 1994.

RBID : pubmed:8052534

Descripteurs français

KwdFr :
- ARN de transfert de la valine (), ARN de transfert de la valine (biosynthèse), Bactériophage T7 (enzymologie), Cinétique, Conformation d'acide nucléique, DNA-directed RNA polymerases (), DNA-directed RNA polymerases (métabolisme), Données de séquences moléculaires, Matrices (génétique), Oligodésoxyribonucléotides, Polyamines (), Polyamines (pharmacologie), Protéines virales, Relation structure-activité, Régions promotrices (génétique), Structure moléculaire, Séquence nucléotidique, Transcription génétique ().
MESH :
- biosynthèse : ARN de transfert de la valine.
- enzymologie : Bactériophage T7.
- métabolisme : DNA-directed RNA polymerases.
- pharmacologie : Polyamines.
- ARN de transfert de la valine, Cinétique, Conformation d'acide nucléique, DNA-directed RNA polymerases, Données de séquences moléculaires, Matrices (génétique), Oligodésoxyribonucléotides, Polyamines, Protéines virales, Relation structure-activité, Régions promotrices (génétique), Structure moléculaire, Séquence nucléotidique, Transcription génétique.

English descriptors

KwdEn :
- Bacteriophage T7 (enzymology), Base Sequence, DNA-Directed RNA Polymerases (drug effects), DNA-Directed RNA Polymerases (metabolism), Kinetics, Molecular Sequence Data, Molecular Structure, Nucleic Acid Conformation, Oligodeoxyribonucleotides, Polyamines (chemistry), Polyamines (pharmacology), Promoter Regions, Genetic, RNA, Transfer, Val (biosynthesis), RNA, Transfer, Val (chemistry), Structure-Activity Relationship, Templates, Genetic, Transcription, Genetic (drug effects), Viral Proteins.
MESH :
- chemical , biosynthesis : RNA, Transfer, Val.
- chemical , chemistry : Polyamines, RNA, Transfer, Val.
- chemical , drug effects : DNA-Directed RNA Polymerases.
- drug effects : Transcription, Genetic.
- enzymology : Bacteriophage T7.
- chemical , metabolism : DNA-Directed RNA Polymerases.
- chemical , pharmacology : Polyamines.
- Base Sequence, Kinetics, Molecular Sequence Data, Molecular Structure, Nucleic Acid Conformation, Oligodeoxyribonucleotides, Promoter Regions, Genetic, Structure-Activity Relationship, Templates, Genetic, Viral Proteins.

Abstract

The influence of nine synthetic polyamines on in vitro transcription with T7 RNA polymerase has been studied. The compounds used were linear or macrocyclic tetra- and hexaamine, varying in their size, shape and number of protonated groups. Their effect was tested on different types of templates, all presenting the T7 RNA promoter in a double-stranded form followed by sequences encoding short transcripts (25 to 35-mers) either on single- or double-stranded synthetic oligodeoxyribonucleotides. All polyamines used stimulate transcription of both types of templates at levels dependent on their size, shape, protonation degree, and concentration. For each compound, an optimal concentration could be defined; above this concentration, transcription inhibition occurred. Highest stimulation (up to 12-fold) was obtained by the largest cyclic compound called [38]N6C10.

DOI: 10.1093/nar/22.14.2784
PubMed: 8052534

Affiliations:

Links toward previous steps (curation, corpus...)

to stream PubMed, to step Corpus: 002872
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Links to Exploration step

pubmed:8052534

Le document en format XML

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<term>ARN de transfert de la valine (biosynthèse)</term>
<term>Bactériophage T7 (enzymologie)</term>
<term>Cinétique</term>
<term>Conformation d'acide nucléique</term>
<term>DNA-directed RNA polymerases ()</term>
<term>DNA-directed RNA polymerases (métabolisme)</term>
<term>Données de séquences moléculaires</term>
<term>Matrices (génétique)</term>
<term>Oligodésoxyribonucléotides</term>
<term>Polyamines ()</term>
<term>Polyamines (pharmacologie)</term>
<term>Protéines virales</term>
<term>Relation structure-activité</term>
<term>Régions promotrices (génétique)</term>
<term>Structure moléculaire</term>
<term>Séquence nucléotidique</term>
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<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Transcription, Genetic</term>
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<front><div type="abstract" xml:lang="en">The influence of nine synthetic polyamines on in vitro transcription with T7 RNA polymerase has been studied. The compounds used were linear or macrocyclic tetra- and hexaamine, varying in their size, shape and number of protonated groups. Their effect was tested on different types of templates, all presenting the T7 RNA promoter in a double-stranded form followed by sequences encoding short transcripts (25 to 35-mers) either on single- or double-stranded synthetic oligodeoxyribonucleotides. All polyamines used stimulate transcription of both types of templates at levels dependent on their size, shape, protonation degree, and concentration. For each compound, an optimal concentration could be defined; above this concentration, transcription inhibition occurred. Highest stimulation (up to 12-fold) was obtained by the largest cyclic compound called [38]N6C10.</div>
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<Abstract><AbstractText>The influence of nine synthetic polyamines on in vitro transcription with T7 RNA polymerase has been studied. The compounds used were linear or macrocyclic tetra- and hexaamine, varying in their size, shape and number of protonated groups. Their effect was tested on different types of templates, all presenting the T7 RNA promoter in a double-stranded form followed by sequences encoding short transcripts (25 to 35-mers) either on single- or double-stranded synthetic oligodeoxyribonucleotides. All polyamines used stimulate transcription of both types of templates at levels dependent on their size, shape, protonation degree, and concentration. For each compound, an optimal concentration could be defined; above this concentration, transcription inhibition occurred. Highest stimulation (up to 12-fold) was obtained by the largest cyclic compound called [38]N6C10.</AbstractText>
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<tree><noCountry><name sortKey="Florentz, C" sort="Florentz, C" uniqKey="Florentz C" first="C" last="Florentz">C. Florentz</name>
<name sortKey="Giege, R" sort="Giege, R" uniqKey="Giege R" first="R" last="Giegé">R. Giegé</name>
<name sortKey="Hosseini, M W" sort="Hosseini, M W" uniqKey="Hosseini M" first="M W" last="Hosseini">M W Hosseini</name>
<name sortKey="Lehn, J M" sort="Lehn, J M" uniqKey="Lehn J" first="J M" last="Lehn">J M Lehn</name>
</noCountry>
<country name="France"><region name="Grand Est"><name sortKey="Frugier, M" sort="Frugier, M" uniqKey="Frugier M" first="M" last="Frugier">M. Frugier</name>
</region>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/PubMed/Checkpoint

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002709 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 002709 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    PubMed
   |étape=   Checkpoint
   |type=    RBID
   |clé=     pubmed:8052534
   |texte=   Synthetic polyamines stimulate in vitro transcription by T7 RNA polymerase.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i   -Sk "pubmed:8052534" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd   \
       | NlmPubMed2Wicri -a MersV1

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration MERS

Synthetic polyamines stimulate in vitro transcription by T7 RNA polymerase.

Synthetic polyamines stimulate in vitro transcription by T7 RNA polymerase.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki