[Inhibition of hepatitis C virus by antisense oligodeoxynucleotide in vitro].
Identifieur interne : 002562 ( PubMed/Checkpoint ); précédent : 002561; suivant : 002563[Inhibition of hepatitis C virus by antisense oligodeoxynucleotide in vitro].
Auteurs : Y. Liu [République populaire de Chine] ; Z. Chen ; N. HeSource :
- Zhonghua yi xue za zhi [ 0376-2491 ] ; 1997.
Descripteurs français
- KwdFr :
- MESH :
- biosynthèse : ARN messager, Antigènes de l'hépatite C.
- cytologie : Lymphocytes T CD4+.
- génétique : Hepacivirus.
- pharmacologie : Oligonucléotides antisens.
- Cellules cultivées, Hepacivirus, Humains, Réplication virale.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : Hepatitis C Antigens, RNA, Messenger.
- cytology : CD4-Positive T-Lymphocytes.
- drug effects : Hepacivirus, Virus Replication.
- genetics : Hepacivirus.
- chemical , pharmacology : Oligonucleotides, Antisense.
- Cells, Cultured, Humans.
Abstract
To study inhibitory effect of antisense oligodeoxynucleotide (asODN) on HCV in vitro.
PubMed: 9772458
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Inhibition of hepatitis C virus by antisense oligodeoxynucleotide in vitro].</title><author><name sortKey="Liu, Y" sort="Liu, Y" uniqKey="Liu Y" first="Y" last="Liu">Y. Liu</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Infectious Disease, Zhejiang Medical University, Hangzhou.</nlm:affiliation><country>République populaire de Chine</country><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName><wicri:orgArea>Institute of Infectious Disease, Zhejiang Medical University</wicri:orgArea></affiliation></author><author><name sortKey="Chen, Z" sort="Chen, Z" uniqKey="Chen Z" first="Z" last="Chen">Z. Chen</name></author><author><name sortKey="He, N" sort="He, N" uniqKey="He N" first="N" last="He">N. He</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1997">1997</date><idno type="RBID">pubmed:9772458</idno><idno type="pmid">9772458</idno><idno type="wicri:Area/PubMed/Corpus">002712</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002712</idno><idno type="wicri:Area/PubMed/Curation">002712</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002712</idno><idno type="wicri:Area/PubMed/Checkpoint">002562</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002562</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Inhibition of hepatitis C virus by antisense oligodeoxynucleotide in vitro].</title><author><name sortKey="Liu, Y" sort="Liu, Y" uniqKey="Liu Y" first="Y" last="Liu">Y. Liu</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Infectious Disease, Zhejiang Medical University, Hangzhou.</nlm:affiliation><country>République populaire de Chine</country><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName><wicri:orgArea>Institute of Infectious Disease, Zhejiang Medical University</wicri:orgArea></affiliation></author><author><name sortKey="Chen, Z" sort="Chen, Z" uniqKey="Chen Z" first="Z" last="Chen">Z. Chen</name></author><author><name sortKey="He, N" sort="He, N" uniqKey="He N" first="N" last="He">N. He</name></author></analytic><series><title level="j">Zhonghua yi xue za zhi</title><idno type="ISSN">0376-2491</idno><imprint><date when="1997" type="published">1997</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>CD4-Positive T-Lymphocytes (cytology)</term><term>Cells, Cultured</term><term>Hepacivirus (drug effects)</term><term>Hepacivirus (genetics)</term><term>Hepatitis C Antigens (biosynthesis)</term><term>Humans</term><term>Oligonucleotides, Antisense (pharmacology)</term><term>RNA, Messenger (biosynthesis)</term><term>Virus Replication (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (biosynthèse)</term><term>Antigènes de l'hépatite C (biosynthèse)</term><term>Cellules cultivées</term><term>Hepacivirus ()</term><term>Hepacivirus (génétique)</term><term>Humains</term><term>Lymphocytes T CD4+ (cytologie)</term><term>Oligonucléotides antisens (pharmacologie)</term><term>Réplication virale ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Hepatitis C Antigens</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>ARN messager</term><term>Antigènes de l'hépatite C</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Lymphocytes T CD4+</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>CD4-Positive T-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Hepacivirus</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Hepacivirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Hepacivirus</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Oligonucléotides antisens</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Oligonucleotides, Antisense</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cells, Cultured</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules cultivées</term><term>Hepacivirus</term><term>Humains</term><term>Réplication virale</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To study inhibitory effect of antisense oligodeoxynucleotide (asODN) on HCV in vitro.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">9772458</PMID><DateCompleted><Year>1998</Year><Month>11</Month><Day>06</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0376-2491</ISSN><JournalIssue CitedMedium="Print"><Volume>77</Volume><Issue>8</Issue><PubDate><Year>1997</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Zhonghua yi xue za zhi</Title><ISOAbbreviation>Zhonghua Yi Xue Za Zhi</ISOAbbreviation></Journal><ArticleTitle>[Inhibition of hepatitis C virus by antisense oligodeoxynucleotide in vitro].</ArticleTitle><Pagination><MedlinePgn>567-70</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To study inhibitory effect of antisense oligodeoxynucleotide (asODN) on HCV in vitro.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">The H9 cells transfected by pCD-HCV, a recombinant HCV containing total HCV structural gene, were treated with two 15-mers phosphorothioate (PS) ODNs complementary (PS-ASON) and homologous (PS-ODN) to HCV core genomic region, which were labeled with digoxin (DIG). Spot blot hybridization was carried out. Treated by the two ODNs, rPS-ODN (a 15-mers PS ODN of random sequence) or PS-ASON were modified with two liposomes (DOTAP and Lipofectin) and calcium phosphate precipitation respectively. With a half-ration, the variation of level of HCV mRNA and HCV antigen expression was observed by RT-PCR and dot ELISA. 3H-TdR adding test was done to observe PS-ASON cytotoxicity.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">PS-ODN and PS-ASON were detected in the H9 cells. The target gene was hybridized to PS-ASON and PS-ODN labeled with DIG. PS-ASON cut down level of HCV mRNA and HCV antigen expression obviously. However, PS-ODN and rPS-ODN did not influence the level of the both. The time-dependent and dose-dependent inhibition of PS-ASON was observed. In contrast to free PS-ASON, both of liposomal PS-ASON showed more highly effective inhibition, but calcium phosphate precipitation-PS-ASON complex did not. The results showed PS-ASON did not influence the H9 cells growth at 10 mumol/L.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">PS-ASON complementary to HCV core gene is asODN and exerts antisense-inhibitory effect on the level of HCV translation obviously, but not on the level of HCV replication and transcription.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Y</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Institute of Infectious Disease, Zhejiang Medical University, Hangzhou.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Z</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>N</ForeName><Initials>N</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Zhonghua Yi Xue Za Zhi</MedlineTA><NlmUniqueID>7511141</NlmUniqueID><ISSNLinking>0376-2491</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018936">Hepatitis C Antigens</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016376">Oligonucleotides, Antisense</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015496" MajorTopicYN="N">CD4-Positive T-Lymphocytes</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016174" MajorTopicYN="N">Hepacivirus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018936" MajorTopicYN="N">Hepatitis C Antigens</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016376" MajorTopicYN="N">Oligonucleotides, Antisense</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014779" MajorTopicYN="N">Virus Replication</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1997</Year><Month>8</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1998</Year><Month>10</Month><Day>17</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1997</Year><Month>8</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">9772458</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Zhejiang</li></region><settlement><li>Hangzhou</li></settlement></list><tree><noCountry><name sortKey="Chen, Z" sort="Chen, Z" uniqKey="Chen Z" first="Z" last="Chen">Z. Chen</name><name sortKey="He, N" sort="He, N" uniqKey="He N" first="N" last="He">N. He</name></noCountry><country name="République populaire de Chine"><region name="Zhejiang"><name sortKey="Liu, Y" sort="Liu, Y" uniqKey="Liu Y" first="Y" last="Liu">Y. Liu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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