[Inhibition of hepatitis C virus by antisense oligodeoxynucleotide in vitro].
Identifieur interne : 002562 ( PubMed/Checkpoint ); précédent : 002561; suivant : 002563[Inhibition of hepatitis C virus by antisense oligodeoxynucleotide in vitro].
Auteurs : Y. Liu [République populaire de Chine] ; Z. Chen ; N. HeSource :
- Zhonghua yi xue za zhi [ 0376-2491 ] ; 1997.
Descripteurs français
- KwdFr :
- MESH :
- biosynthèse : ARN messager, Antigènes de l'hépatite C.
- cytologie : Lymphocytes T CD4+.
- génétique : Hepacivirus.
- pharmacologie : Oligonucléotides antisens.
- Cellules cultivées, Hepacivirus, Humains, Réplication virale.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : Hepatitis C Antigens, RNA, Messenger.
- cytology : CD4-Positive T-Lymphocytes.
- drug effects : Hepacivirus, Virus Replication.
- genetics : Hepacivirus.
- chemical , pharmacology : Oligonucleotides, Antisense.
- Cells, Cultured, Humans.
Abstract
To study inhibitory effect of antisense oligodeoxynucleotide (asODN) on HCV in vitro.
PubMed: 9772458
Affiliations:
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pubmed:9772458Le document en format XML
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<author><name sortKey="Liu, Y" sort="Liu, Y" uniqKey="Liu Y" first="Y" last="Liu">Y. Liu</name>
<affiliation wicri:level="3"><nlm:affiliation>Institute of Infectious Disease, Zhejiang Medical University, Hangzhou.</nlm:affiliation>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Hangzhou</settlement>
<region type="province">Zhejiang</region>
</placeName>
<wicri:orgArea>Institute of Infectious Disease, Zhejiang Medical University</wicri:orgArea>
</affiliation>
</author>
<author><name sortKey="Chen, Z" sort="Chen, Z" uniqKey="Chen Z" first="Z" last="Chen">Z. Chen</name>
</author>
<author><name sortKey="He, N" sort="He, N" uniqKey="He N" first="N" last="He">N. He</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[Inhibition of hepatitis C virus by antisense oligodeoxynucleotide in vitro].</title>
<author><name sortKey="Liu, Y" sort="Liu, Y" uniqKey="Liu Y" first="Y" last="Liu">Y. Liu</name>
<affiliation wicri:level="3"><nlm:affiliation>Institute of Infectious Disease, Zhejiang Medical University, Hangzhou.</nlm:affiliation>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Hangzhou</settlement>
<region type="province">Zhejiang</region>
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<wicri:orgArea>Institute of Infectious Disease, Zhejiang Medical University</wicri:orgArea>
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<author><name sortKey="Chen, Z" sort="Chen, Z" uniqKey="Chen Z" first="Z" last="Chen">Z. Chen</name>
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<author><name sortKey="He, N" sort="He, N" uniqKey="He N" first="N" last="He">N. He</name>
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<series><title level="j">Zhonghua yi xue za zhi</title>
<idno type="ISSN">0376-2491</idno>
<imprint><date when="1997" type="published">1997</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>CD4-Positive T-Lymphocytes (cytology)</term>
<term>Cells, Cultured</term>
<term>Hepacivirus (drug effects)</term>
<term>Hepacivirus (genetics)</term>
<term>Hepatitis C Antigens (biosynthesis)</term>
<term>Humans</term>
<term>Oligonucleotides, Antisense (pharmacology)</term>
<term>RNA, Messenger (biosynthesis)</term>
<term>Virus Replication (drug effects)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (biosynthèse)</term>
<term>Antigènes de l'hépatite C (biosynthèse)</term>
<term>Cellules cultivées</term>
<term>Hepacivirus ()</term>
<term>Hepacivirus (génétique)</term>
<term>Humains</term>
<term>Lymphocytes T CD4+ (cytologie)</term>
<term>Oligonucléotides antisens (pharmacologie)</term>
<term>Réplication virale ()</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Hepatitis C Antigens</term>
<term>RNA, Messenger</term>
</keywords>
<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>ARN messager</term>
<term>Antigènes de l'hépatite C</term>
</keywords>
<keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Lymphocytes T CD4+</term>
</keywords>
<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>CD4-Positive T-Lymphocytes</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Hepacivirus</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Hepacivirus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Hepacivirus</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Oligonucléotides antisens</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Oligonucleotides, Antisense</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Cells, Cultured</term>
<term>Humans</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Cellules cultivées</term>
<term>Hepacivirus</term>
<term>Humains</term>
<term>Réplication virale</term>
</keywords>
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<front><div type="abstract" xml:lang="en">To study inhibitory effect of antisense oligodeoxynucleotide (asODN) on HCV in vitro.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">9772458</PMID>
<DateCompleted><Year>1998</Year>
<Month>11</Month>
<Day>06</Day>
</DateCompleted>
<DateRevised><Year>2006</Year>
<Month>11</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0376-2491</ISSN>
<JournalIssue CitedMedium="Print"><Volume>77</Volume>
<Issue>8</Issue>
<PubDate><Year>1997</Year>
<Month>Aug</Month>
</PubDate>
</JournalIssue>
<Title>Zhonghua yi xue za zhi</Title>
<ISOAbbreviation>Zhonghua Yi Xue Za Zhi</ISOAbbreviation>
</Journal>
<ArticleTitle>[Inhibition of hepatitis C virus by antisense oligodeoxynucleotide in vitro].</ArticleTitle>
<Pagination><MedlinePgn>567-70</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To study inhibitory effect of antisense oligodeoxynucleotide (asODN) on HCV in vitro.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">The H9 cells transfected by pCD-HCV, a recombinant HCV containing total HCV structural gene, were treated with two 15-mers phosphorothioate (PS) ODNs complementary (PS-ASON) and homologous (PS-ODN) to HCV core genomic region, which were labeled with digoxin (DIG). Spot blot hybridization was carried out. Treated by the two ODNs, rPS-ODN (a 15-mers PS ODN of random sequence) or PS-ASON were modified with two liposomes (DOTAP and Lipofectin) and calcium phosphate precipitation respectively. With a half-ration, the variation of level of HCV mRNA and HCV antigen expression was observed by RT-PCR and dot ELISA. 3H-TdR adding test was done to observe PS-ASON cytotoxicity.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">PS-ODN and PS-ASON were detected in the H9 cells. The target gene was hybridized to PS-ASON and PS-ODN labeled with DIG. PS-ASON cut down level of HCV mRNA and HCV antigen expression obviously. However, PS-ODN and rPS-ODN did not influence the level of the both. The time-dependent and dose-dependent inhibition of PS-ASON was observed. In contrast to free PS-ASON, both of liposomal PS-ASON showed more highly effective inhibition, but calcium phosphate precipitation-PS-ASON complex did not. The results showed PS-ASON did not influence the H9 cells growth at 10 mumol/L.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">PS-ASON complementary to HCV core gene is asODN and exerts antisense-inhibitory effect on the level of HCV translation obviously, but not on the level of HCV replication and transcription.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName>
<ForeName>Y</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>Institute of Infectious Disease, Zhejiang Medical University, Hangzhou.</Affiliation>
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<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Z</ForeName>
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<Author ValidYN="Y"><LastName>He</LastName>
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<Language>chi</Language>
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<MedlineJournalInfo><Country>China</Country>
<MedlineTA>Zhonghua Yi Xue Za Zhi</MedlineTA>
<NlmUniqueID>7511141</NlmUniqueID>
<ISSNLinking>0376-2491</ISSNLinking>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D018936">Hepatitis C Antigens</NameOfSubstance>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D015496" MajorTopicYN="N">CD4-Positive T-Lymphocytes</DescriptorName>
<QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016174" MajorTopicYN="N">Hepacivirus</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018936" MajorTopicYN="N">Hepatitis C Antigens</DescriptorName>
<QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName>
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<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
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<MeshHeading><DescriptorName UI="D014779" MajorTopicYN="N">Virus Replication</DescriptorName>
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<affiliations><list><country><li>République populaire de Chine</li>
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<region><li>Zhejiang</li>
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<settlement><li>Hangzhou</li>
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