Competition between the replication initiator DnaA and the sequestration factor SeqA for binding to the hemimethylated chromosomal origin of E. coli in vitro.
Identifieur interne : 002452 ( PubMed/Checkpoint ); précédent : 002451; suivant : 002453Competition between the replication initiator DnaA and the sequestration factor SeqA for binding to the hemimethylated chromosomal origin of E. coli in vitro.
Auteurs : A. Taghbalout [France] ; A. Landoulsi ; R. Kern ; M. Yamazoe ; S. Hiraga ; B. Holland ; M. Kohiyama ; A. MalkiSource :
- Genes to cells : devoted to molecular & cellular mechanisms [ 1356-9597 ] ; 2000.
Descripteurs français
- KwdFr :
- Complexe ORC, Escherichia coli, Facteurs de transcription, Fixation compétitive (génétique), Méthylation de l'ADN (), Origine de réplication (génétique), Plasmides (génétique), Protéines Escherichia coli, Protéines bactériennes (génétique), Protéines bactériennes (métabolisme), Protéines bactériennes (pharmacologie), Protéines de fusion recombinantes (génétique), Protéines de fusion recombinantes (métabolisme), Protéines de la membrane externe bactérienne, Protéines de liaison à l'ADN (génétique), Protéines de liaison à l'ADN (métabolisme), Protéines virales (génétique), Régions promotrices (génétique) (génétique), Réplication de l'ADN (), Réplication de l'ADN (génétique), Site-specific DNA-methyltransferase (adenine-specific) (antagonistes et inhibiteurs), Sites de fixation (génétique), Séquence riche en AT (physiologie), Séquences d'acides nucléiques régulatrices (génétique).
- MESH :
- antagonistes et inhibiteurs : Site-specific DNA-methyltransferase (adenine-specific).
- génétique : Fixation compétitive, Origine de réplication, Plasmides, Protéines bactériennes, Protéines de fusion recombinantes, Protéines de liaison à l'ADN, Protéines virales, Régions promotrices (génétique), Réplication de l'ADN, Sites de fixation, Séquences d'acides nucléiques régulatrices.
- métabolisme : Protéines bactériennes, Protéines de fusion recombinantes, Protéines de liaison à l'ADN.
- pharmacologie : Protéines bactériennes.
- physiologie : Séquence riche en AT.
- Complexe ORC, Escherichia coli, Facteurs de transcription, Méthylation de l'ADN, Protéines Escherichia coli, Protéines de la membrane externe bactérienne, Réplication de l'ADN.
English descriptors
- KwdEn :
- AT Rich Sequence (physiology), Bacterial Outer Membrane Proteins, Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Bacterial Proteins (pharmacology), Binding Sites (genetics), Binding, Competitive (genetics), DNA Methylation (drug effects), DNA Replication (drug effects), DNA Replication (genetics), DNA-Binding Proteins (genetics), DNA-Binding Proteins (metabolism), Escherichia coli, Escherichia coli Proteins, Origin Recognition Complex, Plasmids (genetics), Promoter Regions, Genetic (genetics), Recombinant Fusion Proteins (genetics), Recombinant Fusion Proteins (metabolism), Regulatory Sequences, Nucleic Acid (genetics), Replication Origin (genetics), Site-Specific DNA-Methyltransferase (Adenine-Specific) (antagonists & inhibitors), Transcription Factors, Viral Proteins (genetics).
- MESH :
- chemical , antagonists & inhibitors : Site-Specific DNA-Methyltransferase (Adenine-Specific).
- chemical , genetics : Bacterial Proteins, DNA-Binding Proteins, Recombinant Fusion Proteins, Viral Proteins.
- chemical , metabolism : Bacterial Proteins, DNA-Binding Proteins, Recombinant Fusion Proteins.
- chemical , pharmacology : Bacterial Proteins.
- chemical : Bacterial Outer Membrane Proteins, Escherichia coli Proteins, Origin Recognition Complex, Transcription Factors.
- drug effects : DNA Methylation, DNA Replication.
- genetics : Binding Sites, Binding, Competitive, DNA Replication, Plasmids, Promoter Regions, Genetic, Regulatory Sequences, Nucleic Acid, Replication Origin.
- physiology : AT Rich Sequence.
- Escherichia coli.
Abstract
Following replication initiation, the replication origin (oriC) in Escherichia coli enters a hemimethylated state at Dam methylation sites which are recognized by the SeqA protein. SeqA binds preferentially to hemimethylated GATC sequences of DNA in vitro. SeqA is essential for the synchronous initiation of chromosome replication from oriC copies in vivo.
DOI: 10.1046/j.1365-2443.2000.00380.x
PubMed: 11122375
Affiliations:
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pubmed:11122375Le document en format XML
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Regions, Genetic (genetics)</term><term>Recombinant Fusion Proteins (genetics)</term><term>Recombinant Fusion Proteins (metabolism)</term><term>Regulatory Sequences, Nucleic Acid (genetics)</term><term>Replication Origin (genetics)</term><term>Site-Specific DNA-Methyltransferase (Adenine-Specific) (antagonists & inhibitors)</term><term>Transcription Factors</term><term>Viral Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Complexe ORC</term><term>Escherichia coli</term><term>Facteurs de transcription</term><term>Fixation compétitive (génétique)</term><term>Méthylation de l'ADN ()</term><term>Origine de réplication (génétique)</term><term>Plasmides (génétique)</term><term>Protéines Escherichia coli</term><term>Protéines bactériennes (génétique)</term><term>Protéines bactériennes (métabolisme)</term><term>Protéines bactériennes (pharmacologie)</term><term>Protéines de fusion recombinantes (génétique)</term><term>Protéines de fusion recombinantes 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Replication</term><term>Plasmids</term><term>Promoter Regions, Genetic</term><term>Regulatory Sequences, Nucleic Acid</term><term>Replication Origin</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Fixation compétitive</term><term>Origine de réplication</term><term>Plasmides</term><term>Protéines bactériennes</term><term>Protéines de fusion recombinantes</term><term>Protéines de liaison à l'ADN</term><term>Protéines virales</term><term>Régions promotrices (génétique)</term><term>Réplication de l'ADN</term><term>Sites de fixation</term><term>Séquences d'acides nucléiques régulatrices</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines bactériennes</term><term>Protéines de fusion recombinantes</term><term>Protéines de liaison à l'ADN</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Protéines bactériennes</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Séquence riche en AT</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>AT Rich Sequence</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Complexe ORC</term><term>Escherichia coli</term><term>Facteurs de transcription</term><term>Méthylation de l'ADN</term><term>Protéines Escherichia coli</term><term>Protéines de la membrane externe bactérienne</term><term>Réplication de l'ADN</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Following replication initiation, the replication origin (oriC) in Escherichia coli enters a hemimethylated state at Dam methylation sites which are recognized by the SeqA protein. SeqA binds preferentially to hemimethylated GATC sequences of DNA in vitro. SeqA is essential for the synchronous initiation of chromosome replication from oriC copies in vivo.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11122375</PMID><DateCompleted><Year>2001</Year><Month>02</Month><Day>22</Day></DateCompleted><DateRevised><Year>2019</Year><Month>11</Month><Day>04</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1356-9597</ISSN><JournalIssue CitedMedium="Print"><Volume>5</Volume><Issue>11</Issue><PubDate><Year>2000</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Genes to cells : devoted to molecular & cellular mechanisms</Title><ISOAbbreviation>Genes Cells</ISOAbbreviation></Journal><ArticleTitle>Competition between the replication initiator DnaA and the sequestration factor SeqA for binding to the hemimethylated chromosomal origin of E. coli in vitro.</ArticleTitle><Pagination><MedlinePgn>873-884</MedlinePgn></Pagination><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Following replication initiation, the replication origin (oriC) in Escherichia coli enters a hemimethylated state at Dam methylation sites which are recognized by the SeqA protein. SeqA binds preferentially to hemimethylated GATC sequences of DNA in vitro. SeqA is essential for the synchronous initiation of chromosome replication from oriC copies in vivo.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">We show that: (i) purified SeqA binds AT-rich and 13-mers regions and two DnaA boxes, R1 and M, of hemimethylated oriC. (ii) SeqA inhibits the in vitro replication of a hemimethylated oriC plasmid more efficiently than the fully methylated, (iii) SeqA inhibits competitive binding of DnaA protein to the regions of the hemimethylated oriC plasmid, explaining the mechanism of its inhibitory effect. The inhibition of DnaA binding by SeqA also occurs efficiently on a small hemimethylated oriC fragment containing both R1 and M DnaA boxes, but not the 13-mer region.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">SeqA binds strongly the long region from the AT-rich region to the M DnaA box of the hemimethylated oriC DNA and releases DnaA molecules from the long region.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Taghbalout</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Institut Jacques Monod, CNRS, Université Paris 6-7, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Landoulsi</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Kern</LastName><ForeName>R</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Yamazoe</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Hiraga</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Holland</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Kohiyama</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Malki</LastName><ForeName>A</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Genes Cells</MedlineTA><NlmUniqueID>9607379</NlmUniqueID><ISSNLinking>1356-9597</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001425">Bacterial Outer Membrane Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004268">DNA-Binding Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C066312">DnaA protein, Bacteria</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029968">Escherichia coli Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C114650">OriC chromosomal replication origin</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D051738">Origin Recognition Complex</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011993">Recombinant Fusion Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C089746">SeqA protein, E coli</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014157">Transcription Factors</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.1.1.72</RegistryNumber><NameOfSubstance UI="C058693">Dam methyltransferase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.1.1.72</RegistryNumber><NameOfSubstance UI="D015265">Site-Specific DNA-Methyltransferase (Adenine-Specific)</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.1.1.72</RegistryNumber><NameOfSubstance UI="C499294">dam protein, E coli</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020856" MajorTopicYN="N">AT Rich Sequence</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001425" MajorTopicYN="N">Bacterial Outer Membrane Proteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001665" MajorTopicYN="N">Binding Sites</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001667" MajorTopicYN="N">Binding, Competitive</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019175" MajorTopicYN="N">DNA Methylation</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004261" MajorTopicYN="N">DNA Replication</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004268" MajorTopicYN="N">DNA-Binding Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D029968" MajorTopicYN="N">Escherichia coli Proteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051738" MajorTopicYN="N">Origin Recognition Complex</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011993" MajorTopicYN="N">Recombinant Fusion Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012045" MajorTopicYN="N">Regulatory Sequences, Nucleic Acid</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018741" MajorTopicYN="N">Replication Origin</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015265" MajorTopicYN="N">Site-Specific DNA-Methyltransferase (Adenine-Specific)</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="N">antagonists & inhibitors</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014157" MajorTopicYN="Y">Transcription Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2000</Year><Month>12</Month><Day>21</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>3</Month><Day>3</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2000</Year><Month>12</Month><Day>21</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11122375</ArticleId><ArticleId IdType="pii">gtc380</ArticleId><ArticleId IdType="doi">10.1046/j.1365-2443.2000.00380.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country></list><tree><noCountry><name sortKey="Hiraga, S" sort="Hiraga, S" uniqKey="Hiraga S" first="S" last="Hiraga">S. Hiraga</name><name sortKey="Holland, B" sort="Holland, B" uniqKey="Holland B" first="B" last="Holland">B. Holland</name><name sortKey="Kern, R" sort="Kern, R" uniqKey="Kern R" first="R" last="Kern">R. Kern</name><name sortKey="Kohiyama, M" sort="Kohiyama, M" uniqKey="Kohiyama M" first="M" last="Kohiyama">M. Kohiyama</name><name sortKey="Landoulsi, A" sort="Landoulsi, A" uniqKey="Landoulsi A" first="A" last="Landoulsi">A. Landoulsi</name><name sortKey="Malki, A" sort="Malki, A" uniqKey="Malki A" first="A" last="Malki">A. Malki</name><name sortKey="Yamazoe, M" sort="Yamazoe, M" uniqKey="Yamazoe M" first="M" last="Yamazoe">M. Yamazoe</name></noCountry><country name="France"><noRegion><name sortKey="Taghbalout, A" sort="Taghbalout, A" uniqKey="Taghbalout A" first="A" last="Taghbalout">A. Taghbalout</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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