Defining T cell receptors which recognise the immunodominant epitope of the gastric autoantigen, the H/K ATPase beta-subunit.
Identifieur interne : 002450 ( PubMed/Checkpoint ); précédent : 002449; suivant : 002451Defining T cell receptors which recognise the immunodominant epitope of the gastric autoantigen, the H/K ATPase beta-subunit.
Auteurs : H D De Silva ; F. Alderuccio ; B H Toh ; I R Van Driel ; P A GleesoSource :
- Autoimmunity [ 0891-6934 ] ; 2000.
Descripteurs français
- KwdFr :
- Animaux, Autoantigènes (immunologie), Cartographie épitopique, Données de séquences moléculaires, Déterminants antigéniques des lymphocytes T (immunologie), Estomac (enzymologie), Estomac (immunologie), Femelle, H(+)-K(+)-Exchanging ATPase (immunologie), Hybridomes, Mâle, Peptides (immunologie), Récepteur lymphocytaire T antigène, alpha-bêta (génétique), Récepteur lymphocytaire T antigène, alpha-bêta (immunologie), Souris, Souris de lignée BALB C, Souris de lignée C57BL, Souris transgéniques, Séquence d'acides aminés, Séquence nucléotidique, Épitopes immunodominants (immunologie).
- MESH :
- enzymologie : Estomac.
- génétique : Récepteur lymphocytaire T antigène, alpha-bêta.
- immunologie : Autoantigènes, Déterminants antigéniques des lymphocytes T, Estomac, H(+)-K(+)-Exchanging ATPase, Peptides, Récepteur lymphocytaire T antigène, alpha-bêta, Épitopes immunodominants.
- Animaux, Cartographie épitopique, Données de séquences moléculaires, Femelle, Hybridomes, Mâle, Souris, Souris de lignée BALB C, Souris de lignée C57BL, Souris transgéniques, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Autoantigens (immunology), Base Sequence, Epitope Mapping, Epitopes, T-Lymphocyte (immunology), Female, H(+)-K(+)-Exchanging ATPase (immunology), Hybridomas, Immunodominant Epitopes (immunology), Male, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Transgenic, Molecular Sequence Data, Peptides (immunology), Receptors, Antigen, T-Cell, alpha-beta (genetics), Receptors, Antigen, T-Cell, alpha-beta (immunology), Stomach (enzymology), Stomach (immunology).
- MESH :
- chemical , genetics : Receptors, Antigen, T-Cell, alpha-beta.
- chemical , immunology : Autoantigens, Epitopes, T-Lymphocyte, H(+)-K(+)-Exchanging ATPase, Immunodominant Epitopes, Peptides, Receptors, Antigen, T-Cell, alpha-beta.
- enzymology : Stomach.
- immunology : Stomach.
- Amino Acid Sequence, Animals, Base Sequence, Epitope Mapping, Female, Hybridomas, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Transgenic, Molecular Sequence Data.
Abstract
We have previously shown that autoimmune gastritis can be elicited in mice by immunisation with the gastric parietal cell H/K ATPase alphabeta heterodimer, and, furthermore, have identified the H/K ATPase beta-subunit epitope, H/Kbeta253-277 as the dominant epitope of the gastric H/K ATPase. Using gastric H/K ATPase-immunised mice, here we have generated two T cell hybridomas specific for the H/Kbeta253-277 peptide, namely 4B11.F4.5 and 1E4.C1. Hybridoma 4B11.F4.5 uses Valpha8 and Vbeta8.2 TCR chains and 1E4.C1 uses Valpha9 and V1beta8.3 chains. Although both hybridomas are specific for H/Kbeta253-277, T cell assays using overlapping 14-mers of the 25-mer epitope showed that the two autoreactive TCRs recognise different regions of the 25-mer. The TCR from 1E4.C1 has been used to generate a TCR beta-chain transgenic mouse. >80% of peripheral CD4+ T cells utilise the Vbeta8.3 transgene. As expected, 1E4-TCR beta-chain transgenic mice are susceptible to neonatal thymectomy induced autoimmune gastritis. While none of the 1E4-TCR beta chain transgenic mice spontaneously developed a destructive gastritis, a minority (20%) of the transgenic mice developed a non-invasive and non-destructive gastritis. This suggests that the pathogenic T cells are maintained in a tolerant state in the periphery of the transgenic mice.
DOI: 10.3109/08916930108994104
PubMed: 11204248
Affiliations:
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Alderuccio</name></author><author><name sortKey="Toh, B H" sort="Toh, B H" uniqKey="Toh B" first="B H" last="Toh">B H Toh</name></author><author><name sortKey="Van Driel, I R" sort="Van Driel, I R" uniqKey="Van Driel I" first="I R" last="Van Driel">I R Van Driel</name></author><author><name sortKey="Gleeso, P A" sort="Gleeso, P A" uniqKey="Gleeso P" first="P A" last="Gleeso">P A Gleeso</name></author></analytic><series><title level="j">Autoimmunity</title><idno type="ISSN">0891-6934</idno><imprint><date when="2000" type="published">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Autoantigens (immunology)</term><term>Base Sequence</term><term>Epitope Mapping</term><term>Epitopes, T-Lymphocyte (immunology)</term><term>Female</term><term>H(+)-K(+)-Exchanging ATPase (immunology)</term><term>Hybridomas</term><term>Immunodominant Epitopes (immunology)</term><term>Male</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Mice, Inbred C57BL</term><term>Mice, Transgenic</term><term>Molecular Sequence Data</term><term>Peptides (immunology)</term><term>Receptors, Antigen, T-Cell, alpha-beta (genetics)</term><term>Receptors, Antigen, T-Cell, alpha-beta (immunology)</term><term>Stomach (enzymology)</term><term>Stomach (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Autoantigènes (immunologie)</term><term>Cartographie épitopique</term><term>Données de séquences moléculaires</term><term>Déterminants antigéniques des lymphocytes T (immunologie)</term><term>Estomac (enzymologie)</term><term>Estomac (immunologie)</term><term>Femelle</term><term>H(+)-K(+)-Exchanging ATPase (immunologie)</term><term>Hybridomes</term><term>Mâle</term><term>Peptides (immunologie)</term><term>Récepteur lymphocytaire T antigène, alpha-bêta (génétique)</term><term>Récepteur lymphocytaire T antigène, alpha-bêta (immunologie)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Souris de lignée C57BL</term><term>Souris transgéniques</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Épitopes immunodominants (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Receptors, Antigen, T-Cell, alpha-beta</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Autoantigens</term><term>Epitopes, T-Lymphocyte</term><term>H(+)-K(+)-Exchanging ATPase</term><term>Immunodominant Epitopes</term><term>Peptides</term><term>Receptors, Antigen, T-Cell, alpha-beta</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Estomac</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Stomach</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Récepteur lymphocytaire T antigène, alpha-bêta</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Autoantigènes</term><term>Déterminants antigéniques des lymphocytes T</term><term>Estomac</term><term>H(+)-K(+)-Exchanging ATPase</term><term>Peptides</term><term>Récepteur lymphocytaire T antigène, alpha-bêta</term><term>Épitopes immunodominants</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Stomach</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Epitope Mapping</term><term>Female</term><term>Hybridomas</term><term>Male</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Mice, Inbred C57BL</term><term>Mice, Transgenic</term><term>Molecular Sequence Data</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cartographie épitopique</term><term>Données de séquences moléculaires</term><term>Femelle</term><term>Hybridomes</term><term>Mâle</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Souris de lignée C57BL</term><term>Souris transgéniques</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have previously shown that autoimmune gastritis can be elicited in mice by immunisation with the gastric parietal cell H/K ATPase alphabeta heterodimer, and, furthermore, have identified the H/K ATPase beta-subunit epitope, H/Kbeta253-277 as the dominant epitope of the gastric H/K ATPase. Using gastric H/K ATPase-immunised mice, here we have generated two T cell hybridomas specific for the H/Kbeta253-277 peptide, namely 4B11.F4.5 and 1E4.C1. Hybridoma 4B11.F4.5 uses Valpha8 and Vbeta8.2 TCR chains and 1E4.C1 uses Valpha9 and V1beta8.3 chains. Although both hybridomas are specific for H/Kbeta253-277, T cell assays using overlapping 14-mers of the 25-mer epitope showed that the two autoreactive TCRs recognise different regions of the 25-mer. The TCR from 1E4.C1 has been used to generate a TCR beta-chain transgenic mouse. >80% of peripheral CD4+ T cells utilise the Vbeta8.3 transgene. As expected, 1E4-TCR beta-chain transgenic mice are susceptible to neonatal thymectomy induced autoimmune gastritis. While none of the 1E4-TCR beta chain transgenic mice spontaneously developed a destructive gastritis, a minority (20%) of the transgenic mice developed a non-invasive and non-destructive gastritis. This suggests that the pathogenic T cells are maintained in a tolerant state in the periphery of the transgenic mice.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11204248</PMID><DateCompleted><Year>2001</Year><Month>04</Month><Day>26</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0891-6934</ISSN><JournalIssue CitedMedium="Print"><Volume>33</Volume><Issue>1</Issue><PubDate><Year>2000</Year></PubDate></JournalIssue><Title>Autoimmunity</Title><ISOAbbreviation>Autoimmunity</ISOAbbreviation></Journal><ArticleTitle>Defining T cell receptors which recognise the immunodominant epitope of the gastric autoantigen, the H/K ATPase beta-subunit.</ArticleTitle><Pagination><MedlinePgn>1-14</MedlinePgn></Pagination><Abstract><AbstractText>We have previously shown that autoimmune gastritis can be elicited in mice by immunisation with the gastric parietal cell H/K ATPase alphabeta heterodimer, and, furthermore, have identified the H/K ATPase beta-subunit epitope, H/Kbeta253-277 as the dominant epitope of the gastric H/K ATPase. Using gastric H/K ATPase-immunised mice, here we have generated two T cell hybridomas specific for the H/Kbeta253-277 peptide, namely 4B11.F4.5 and 1E4.C1. Hybridoma 4B11.F4.5 uses Valpha8 and Vbeta8.2 TCR chains and 1E4.C1 uses Valpha9 and V1beta8.3 chains. Although both hybridomas are specific for H/Kbeta253-277, T cell assays using overlapping 14-mers of the 25-mer epitope showed that the two autoreactive TCRs recognise different regions of the 25-mer. The TCR from 1E4.C1 has been used to generate a TCR beta-chain transgenic mouse. >80% of peripheral CD4+ T cells utilise the Vbeta8.3 transgene. As expected, 1E4-TCR beta-chain transgenic mice are susceptible to neonatal thymectomy induced autoimmune gastritis. While none of the 1E4-TCR beta chain transgenic mice spontaneously developed a destructive gastritis, a minority (20%) of the transgenic mice developed a non-invasive and non-destructive gastritis. This suggests that the pathogenic T cells are maintained in a tolerant state in the periphery of the transgenic mice.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>De Silva</LastName><ForeName>H D</ForeName><Initials>HD</Initials><AffiliationInfo><Affiliation>Department of Pathology and Immunology Monash University Medical School, Melbourne, Victoria, Austrialia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Alderuccio</LastName><ForeName>F</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Toh</LastName><ForeName>B H</ForeName><Initials>BH</Initials></Author><Author ValidYN="Y"><LastName>van Driel</LastName><ForeName>I R</ForeName><Initials>IR</Initials></Author><Author ValidYN="Y"><LastName>Gleeso</LastName><ForeName>P A</ForeName><Initials>PA</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType 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UI="D017506">H(+)-K(+)-Exchanging ATPase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001324" MajorTopicYN="N">Autoantigens</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018604" MajorTopicYN="N">Epitope Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018984" MajorTopicYN="N">Epitopes, T-Lymphocyte</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017506" MajorTopicYN="N">H(+)-K(+)-Exchanging ATPase</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006825" MajorTopicYN="N">Hybridomas</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016056" MajorTopicYN="N">Immunodominant Epitopes</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008810" MajorTopicYN="N">Mice, Inbred C57BL</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008822" MajorTopicYN="N">Mice, Transgenic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010455" MajorTopicYN="N">Peptides</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016693" MajorTopicYN="N">Receptors, Antigen, T-Cell, alpha-beta</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013270" MajorTopicYN="N">Stomach</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2001</Year><Month>2</Month><Day>24</Day><Hour>12</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>5</Month><Day>1</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2001</Year><Month>2</Month><Day>24</Day><Hour>12</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11204248</ArticleId><ArticleId IdType="doi">10.3109/08916930108994104</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Alderuccio, F" sort="Alderuccio, F" uniqKey="Alderuccio F" first="F" last="Alderuccio">F. Alderuccio</name><name sortKey="De Silva, H D" sort="De Silva, H D" uniqKey="De Silva H" first="H D" last="De Silva">H D De Silva</name><name sortKey="Gleeso, P A" sort="Gleeso, P A" uniqKey="Gleeso P" first="P A" last="Gleeso">P A Gleeso</name><name sortKey="Toh, B H" sort="Toh, B H" uniqKey="Toh B" first="B H" last="Toh">B H Toh</name><name sortKey="Van Driel, I R" sort="Van Driel, I R" uniqKey="Van Driel I" first="I R" last="Van Driel">I R Van Driel</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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